Quantitative observation by enzyme cytochemistry of human rectal adenocarcinoma cells killed by LAK cells

Activity of succinic dehydrogenase(SDH)and acid phosphatase(AcPase)of effector-target cells during the process of LAK cells killing HR8348 cells was estimated by enzyme cyto-chemistry technique.SHD positive granules and AePase gray level were assayed with MIAS-300image analyser.The results showed:(1)After cocultivation of effector and target cells for vari-ous times,the activity of AcPase of HR8348 cells was apparently higher than that of the controlgroup,and it increased following prolonged coincubation.SDH activity of target cells increasedmarkedly within 30 and 60 rain cocultivation,but became low after 90 min treatment.(2)Ac-Pase content within LAK cells at 60,90,120,180 and 240 rain cocultivation was significantlyhigher than that of control group(P<0.01).The phenomenon of high AcPase and SDH activitywithin effector-target cells indicates that the function of the two types of cells was in an activestate.At the early stage of effector-target combining,the increase of SDH with HR8348 cellsmay be related to defensive function of the target cells.Higher AcPase activity of target cells in-dicates the activation of lysosomal enzyme which serves as the material basis for autolysis of thecells.     

LightCycler实时监测PCR定量分析血清HBV DNA

目的 检验LightCycler实时监测PCR（real-time detection PCR,RTD-PCR)对血清中HBV DNA定量检测的灵敏性和可重复性，探讨HBV血清标志物与HBV DNA定量的关系。方法 HBV定量按深圳匹基公司乙肝PCR荧光检测试剂盒使用说明，对乙肝标志物已明确的773例血清中HBV DNA定量结果进行统计分析。结果 实时监测PCR对血清中HBV DNA定量检测的灵敏性高，可检测低至1000拷贝／ml血清；可重复性好，批间误差＜20％；各种标志物类型的血清HBV DNA含量分布情况表明，HBV血清标志物中HBeAg与HBV DNA含量有明显的关系，一般HBeAg阳性血清HBV DNA含量较高，但也有相当一部分例外。结论 LightCycler实时监测PCR对血清中HBV DNA定量检测灵敏性高可重复性好；仅根据HBV血清标志物往往不能确定乙肝患者HBV DNA复制水平的高低，HBV DNA定量检测具有十分重要的临床意义。     

Comparison of Minimum Alveolar Concentration between Intravenous Isoflurane Lipid Emulsion and Inhaled Isoflurane in Dogs

Background: As in inhaled isoflurane anesthesia, when isoflurane lipid emulsion (ILE; 8%, vol/vol) is intravenously administered, the primary elimination route is through the lungs. This study was designed to determine the minimum alveolar concentration (MAC) and the time course of washout of isoflurane for intravenously infused ILE by monitoring end-tidal isoflurane concentration.

Methods: Twelve healthy adult mongrel dogs were assigned randomly to an intravenous anesthesia group with 8% ILE or to an inhalation anesthesia group with isoflurane vapor. An up-and-down method and stimulation of tail clamping were used to determine MAC of 8% ILE by intravenous injection in the intravenous anesthesia group and MAC by the inhaled approach in the inhalation anesthesia group, respectively. Isoflurane concentration and partial pressure in end-tidal gas, femoral arterial blood, and jugular venous blood were measured simultaneously just before each tail clamping and during washout.

Results: The induction time in the intravenous anesthesia group (105 +/- 24 s) was shorter than that in the inhalation anesthesia group (378 +/- 102 s; P < 0.01). MAC of 8% ILE by intravenous injection (1.12 +/- 0.18%) was significantly less than MAC by the inhaled approach (1.38 +/- 0.16%; P < 0.05). No significant difference was found between the two groups in the time course of washout of isoflurane.     

长沙市小学生焦虑障碍现状调查

目的　初步了解小学儿童焦虑障碍的患病现况。方法　使用焦虑性情绪障碍筛查表 (SCARED)调查长沙市某小学 2～ 6年级的 5 6 5名小学生 ,并对量表总分≥划界分 (2 3分 )的学生进行面谈。结果　在 5 6 5名小学生 (男生2 90名 ,女生 2 75名 )中 ,SCARED筛查阳性的有 14 0名 ,占总人数的 2 4 78%。使用中国精神障碍分类与诊断标准 (第三版 ) (CCMD 3)对筛查阳性者进行面谈 ,发现符合焦虑障碍诊断标准的 32例 (男 15 ,女 17) ,患病率为 5 6 6 % ,其中儿童分离性焦虑症 7例 ,患病率为 1 2 4 % ;儿童广泛焦虑症 11例 (1 95 % ) ;儿童恐惧症 10例 (1 77% ) ;儿童社交恐惧症 14例(2 4 8% ) ;有 8例存在 2～ 3种焦虑障碍共病 ;无 1例诊断为学校恐怖症、选择性缄默症和惊恐发作。结论　在儿童中焦虑情绪存在较普遍 ,这对儿童的学习、行为、自我意识造成不良影响 ,应予以积极干预。     

用酶联免疫吸附术观察向附睾注射鱼肝油酸钠后抗精子抗体的变化

本研究用酶联免疫吸附术观察8只家兔在附睾注射鱼肝油酸钠后血清及精浆中的抗精子抗体。血清抗精子抗体阳性率为37.5～50.0%,最早检出率为术后2个月,6～7月达高峰。精浆抗精子抗体阳性率为25%,最早检出率为术后4个月,且此时己达高峰。与输精管结扎资料相比,发现血清抗精子抗体检出率偏低,而精浆相应抗体偏高,表明向附睾注射鱼肝油酸钠引起的免疫反应有别于结扎,以生殖道局部为主。     

大剂量地塞米松及SOD在实验性脑损伤中的治疗作用

大剂量地塞米松和ＳＯＤ是外伤性脑继发性损害药物治疗的新方案。本实验在豚鼠局灶性脑损伤模型上试用该两种药物，通过脑含水量、灶周伊文斯兰渗出、血ＣＫ、Ｃａ和ＬＤＨ含室及光镜和电镜病理检查等指标。     

热休克反应对大鼠油酸肺保护作用的机制研究

未研究发现，经热休克处理的大鼠注油酸后，肺组织损伤明显减轻，Ｗｅｓｔｅｒｎ印迹分析显示热休克使大鼠肺组织中ＨＳＰ７０含量明显增多；Ｎｏｒｔｈｅｒｎ印迹分析证实此乃由于ＨＳＰ７０ｍＲＮＡ转录增加所致。说明热休克处理使肺组织ＨＳＰ７０基因表达增强；提示热休克反应对大鼠油酸肺的保护作用与肺组织细胞中ＨＳＰ７０基因表达增强及与ＨＳＰ７０增多有关。     

末梢血管收缩反应预测气管插管血液动力学变化

目的通过末梢血管收缩反应预测气管插管引起的血液动力学变化,提供个体化气管插管所需的麻醉深度。方法末梢血管收缩反应通过强直电刺激(50 Hz、50 mA持续5 s)诱发的末梢灌注指数(tip perfusion index,TPI)变化进行监测。24例ASAⅠ~Ⅱ级颅脑外科择期手术患者,麻醉诱导:咪唑安定0.06 mg/kg、芬太尼4μg/kg,丙泊酚0.25 mg.kg-1.min-1恒速输注。意识消失后每间隔1分钟电刺激一次,记录每次电刺激前后TPI的减少值(△TPI)和插管前后SBP、DBP和HR的增加值(△SBP、△DBP和△HR)。观察组(n=12),△TPI<10%刺激前值时行气管插管;对照组(n=11),不考虑△TPI的变化而根据操作者的经验决定插管时机。记录诱导前、插管前、插管后即刻、1、2和3 min时BIS、SBP、DBP和HR的值。结果插管即刻,观察组比对照组丙泊酚输注时间长(P<0.01)、用量大(P<0.01);插管后每一时间点,对照组SBP、DBP和HR都比观察组高(P<0.01)。组内比较:对照组插管前后SBP、DBP和HR明显增高(P<0.01);观察组插管前后各指标无明显变化。电刺激诱发的△TPI分别与插管引起的△SBP、△DBP和△HR成正相关(r=0.672、0.434和0.236,P<0.01)。结论电刺激诱发的末梢血管收缩反应性可有效预测气管插管引起的血液动力学变化幅度。     

高密度cDNA微阵列技术检测乳腺癌差异表达基因

目的 描绘乳腺癌的基因差异表达谱，以寻找乳腺癌新的肿瘤相关候选基因和分子标志。方法 用含14000个cDNA克隆的表达微阵列检测乳腺癌组织、乳腺癌旁组织及乳腺非癌组织的mRNA表达，并分析其表达的差异。结果 乳腺癌组织与乳腺非癌组织比较有80个差异表达基因，乳腺癌组织与乳腺癌旁组织比较有57个差异表达基因，乳腺癌旁组织与乳腺非癌组织比较有29个差异表达基因，在乳腺癌组织与乳腺非癌组织和乳腺癌旁组织比较中有14个差异表达基因是一致的。结论 用微阵列技术对乳腺癌的肿瘤相关基因和分子标志作初步探索，为了解中国女性乳腺癌发生发展的分子机制、发展新的诊断和治疗手段提供了信息。