Effect of Radix Paeoniae Rubra on the expression of HO-1 and iHOS in rats with endotoxin-induced acute lung injury

Objective ： To investigate the effect of Radix Paeoniae Rubra （RPR） on the expression of heme oxygenase （ HO- 1 ） and induced nitric oxide synthase （ iNOS ） in endotoxin- induced acute lung injury in rats and its protective mechanism. Methods： Forty Wistar rats were divided randomly into 5 groups with 8 rats in each group： saline control group （NS group ）, lipopolysaccharide group （LPS group）, RPR-treatment group, RPR-prevention group and Hemin group. The effect of RPR on protein content, the ratio of neutrophiles in bronchoalveolar lavage fluid, malondialdehyde （MDA） content in the lung and the activity of serum NO were observed. Arterial blood was drawn for blood-gas analysis. The expression of HO-1 and iNOS in lung tissues was detected by immunohistochemitry and morphometry computer image analysis. The histological changes of the lung were observed under fight microscope. Results： Compared with that in NS group, the expression of HO-1 and iNOS was markedly increased in LPS group （P 〈0.01）. In RPR-treatment, RPR-prevention, and Hemin groups, the expression of iNOS was significantly lower, while the expression of HO-1 was higher than that in LPS group （P〈0.05）. The protein content, the ratio of neutrophiles in bronchouiveolar lavage fluid, the content of MDA and the activity of serum NO in LPS group were significantly higher than those in NS group （ P〈0.01 ）. There was a significant decrease in the level of arterial bicarbonate and partial pressure of oxygen in the LPS group （P 〈0.01）; these parameters of lung injury however, were significantly lower in RPR-treatment, RPR- prevention, and Heroin groups than LPS group （P〈0.05 or P〈0.01 ）. The pathologic changes of lung tissues were substantially attenuated in RPR-treatment, RPR- prevention, and Hemin groups than LPS group. Conclusions ： The high expression of HO-1 reflects an important protective function of the body during lipopolysaccharide-induced acute lung injury. The acute lung injury is related to the inhibition of iNOS expression and the induction of HO-1 expression.     

胃癌组织中细胞FLICE抑制蛋白基因的表达研究

目的　本研究检测细胞FLICE抑制蛋白 (c-FLIP)基因在胃癌中的表达及其与临床病理特征的关系,初步探讨c-FLIP在胃癌发生、发展中的意义。方法　收集 48例胃癌及相应癌旁正常组织标本,以免疫组化、Western印迹法检测c FLIP蛋白表达;半定量RT PCR法检测c FLIPmRNA表达;原位末端标记法检测胃癌细胞凋亡指数。结果　胃癌及癌旁正常组织均表达c FLIPmRNA,平均相对吸光度值分别为(0. 59±0. 16)和 ( 0. 24±0. 13 ),前者显著高于后者 (P<0. 01 )。免疫组化结果表明c FLIP蛋白在胃癌中表达的阳性率为 100% (48 /48),且 68. 8% (33 /48)呈强阳性表达,而在癌旁正常组织中的阳性率为 75%,且未见强阳性表达,癌组织中的表达水平 ( 6. 93±0. 58 )显著高于癌旁正常组织(3. 19±0. 26,P<0. 01)。较低表达c FLIP蛋白的癌组织其平均凋亡指数 [ (2. 96±0. 15)% ]明显高于高表达c FLIP的癌组织[ (1. 36±0. 11)%,P<0. 01]。另外,在有淋巴结转移组中c FLIPmRNA(0. 64±0. 18)和蛋白(7. 15±0. 63)的表达水平明显高于无淋巴结转移组的c FLIPmRNA( 0. 52±0. 13,P<0. 05)和蛋白水平(6. 69±0. 47,P<0. 01)。Western印迹分析表明,胃癌组织中长型和短型c FLIP蛋白的水平分别为癌旁正常组织的 2. 6倍(P<0. 01)和 2. 8倍 (P<0. 01     

Experimental Study on the Antibacterial Effect of Origanum Volatile Oil on Dysentery Bacilli In Vivo and In Vitro

To observe the germistatic and germicidal effects of origanum volatile oil (OVI) on the dysentery bacteria, the abdominal cavity of mice was infected with Shigella sonne (Sh. sonnei) and Shigella flexneri (Sh. flexneri) F2a. After OVI was given to the mice via gastric lavage, the effects of OVI on the infected mice were observed. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) for dysentery bacteria were determined in vitro. The resuits showed that origanum volatile oil showed obvious protective effect on mice infected with Sh. sonnei and Sh. flexneri F2a. and it had germistatic and germicidal effects on dysentry bacteria. We are led to conclude that origanum volatile oil is an effective medicine against the infection of dysentery bacteria.     

Protective effects of hypovolemic hypotension preconditioning on cardiopulmonary function after myocardium ischemia／reperfusion injury

RDepartmentofAnesthesiology ,RenminHospital,WuhanUniversity,Wuhan 4 30 0 6 0 ,China (ChenXJ,WangX ,XiaZYandLuoT)DepartmentofCardiacSurgery ,RenminHospital,WuhanUniversity ,Wuhan 4 30 0 6 0 ,China (TuZF) Correspondingauthor:Tel:86 2 7 880 82 4 78,E mail:xue0 30 8@mail.tom .com .cnecentlyischemiapreconditioninghasprovedtoamelioratethesubsequentsustainedmyocardiumischemia reperfusioninjury .1,2 Someexperimentsreportedthatremoteorgansischemiapreconditioning(RPC) ,suchasinkidney ,can…     

缺血预处理对兔主动脉阻断后脊髓一氧化氮、后肢神经功能和肌电图的影响

目的　探讨缺血预处理 (IPC)对缺血预处理对兔主动脉阻断后脊髓功能和一氧化氮(NO)的影响。方法　 2 4只日本大白兔随机分为假手术组 (A组 )、缺血再灌注组 (B组 )和IPC保护组 (C组 ) ,每组 8只。分别于首次预处理即刻 (C 40 )、缺血即刻 (I0 )、缺血 45min(I45)、再灌注后 60min(R60 )和术后 7d处死动物前即刻 (R7d)采血检测血清和R7d脊髓组织NO的浓度。术后观察后肢神经功能的评分、后肢针电极肌电图 (EMG)和脊髓组织病理学的改变。结果　缺血再灌注损伤后B组血清NO浓度较缺血前和A、C组对应时点值显著升高 (P <0 .0 1)。C组R7d血清NO浓度明显低于其他时点及A组R7d测定值 (P <0 .0 5或 0 .0 1)。B组脊髓组织NO浓度显著高于A、C组(P <0 .0 1)。B组后肢神经功能和脊髓病理学评分均显著性低于A、C组 (P <0 .0 5或 0 .0 1) ,其后肢EMG亦较C组有显著性病理改变 (P <0 .0 1)。结论　IPC对家兔主动脉阻断后脊髓缺血再灌注损伤有良好的保护作用 ,其保护作用机制与抑制NO的生成有关。     

小剂量茶碱对哮喘患者诱导痰中嗜酸粒细胞 Bcl-2、Bax蛋白表达的影响

目的 :研究小剂量茶碱对哮喘患者气道嗜酸粒细胞 (eosinophils ,Eos)Bcl 2、Bax蛋白表达的影响。方法 :选择 2 2名哮喘患者口服茶碱缓释片 (舒弗美 ,2 0 0mg·12h-1,平均血浆药物浓度 7.5mg·L-1)治疗 4周 ,观察治疗前后各项指标的变化。结果 :使用茶碱治疗后 ,Eos%明显低于治疗前 (2 9.0 5± 8.30vs 2 2 .5 4± 10 .32 ,P <0 .0 1) ;痰涂片Bcl 2 (+)Eos%治疗后显著低于治疗前 (4 .82± 2 .82vs 7.14± 3.5 1,P <0 .0 1) ;Bax(+)Eos%治疗后显著高于治疗前 (6 .0 9± 3.2 3vs 1.5 9± 1.4 0 ,P <0 .0 1) ;哮喘患者的症状明显好转 ,即哮喘症状评分好转 (5 .12± 1.4 7vs 7.17± 1.4 3,P <0 .0 5 ) ;肺功能明显改善。结论 :小剂量茶碱可使哮喘患者诱导痰中Eos的Bcl 2蛋白表达水平降低 ,Bax蛋白表达水平上升。提示哮喘患者诱导痰中Eos的Bcl 2蛋白表达水平可能作为衡量哮喘严重程度的一项指标 ;茶碱可能通过影响气道Eos的凋亡而发挥抗炎作用 ,其机制可能是通过在Eos中凋亡蛋白的表达水平来实现的。     

儿茶酚胺依赖性多形室速的心率变异性及其β-受体阻滞剂干预后的变化

目的:探讨心率变异在儿茶酚胺依赖性多形室速(catecholaminergic polymorphic ventricular tachycardia,CPVT)危险分层中的应用价值,及其β-受体阻滞剂干预后的变化。方法:根据不同预后对22例(未发猝死组18例,猝死组4例)CPVT患者进行HRV时域指标分析,与正常对照组比较;并比较β-受体阻滞剂干预前、后HRV时域指标的变化。结果:22例CPVT患者HRV各项时域指标均显著低于正常对照组(P<0.001),猝死组显著低于未发猝死组(P<0.001);β-受体阻滞剂可以使其HRV时域指标显著升高(P<0.05)。结论:HRV可以作为CPVT患者发生心脏性猝死的独立预测指标。     

卵泡穿刺结合人工授精治疗多囊卵巢综合征不育

目的探讨一种有效治疗多囊卵巢综合征(PCOS)合并不育的方法。方法对12例PCOS患者15个周期采用促性腺激素刺激卵泡发育,配合经阴道B超下卵泡穿刺术释放卵母卵泡,再行人工授精。结果15个周期中妊娠4例,妊娠率为27%;治疗后第2次月经周期中1例自然妊娠,其他病例在3个月内月经恢复正常,但之后5例月经周期延长,2例复发闭经;患者血黄体生成素(LH)和睾酮(T)值治疗后明显低于治疗前(P<0.05)。结论卵泡穿刺结合人工授精,能迅速阻断PCOS的病理环节,具有创伤较小、疗效确切、简便等优点,可作为治疗PCOS不育的一种选择。     

自体原位移植肾缺血再灌注损伤模型的建立与环孢素A对移植肾的保护作用

目的建立一种新的移植肾缺血再灌注损伤(IRI)动物实验模型并寻找一种新的移植肾保护方法。方法40只兔随机分为A组(生理盐水对照组)、B组(单纯灌注液组)、C组[环孢素A(CsA)+灌注液组,CsA浓度为0.01‰,W/V]、D组(CsA+灌注液组,CsA浓度为0.03‰,W/V)。用套管针穿刺左肾动、静脉,灌注肾脏。原位低温保存2h后,切除右肾并开放左肾血流使左肾复灌。于术后6、24h分别取血标本,24h后取左肾标本,测定血尿素氮(BUN)、血肌酐(Cr),TUNEL法检测分析左肾小管上皮细胞凋亡率,免疫组织化学法测定左肾组织HSP70的表达。结果D组6、24hBUN为(8.60±0.25)mmol/L、(7.99±0.14)mmol/L;Cr为(79.90±1.37)μmol/L、(78.98±1.66)μmol/L,细胞凋亡指数为0.075±0.035,明显低于其他各组;HSP70表达为(0.211±0.025)μm2,明显高于其他各组。结论本模型能客观反映移植肾缺血再灌注损伤状态,0.03‰CsA复合肾灌注液能明显降低移植肾缺血再灌注损伤。     

尼美舒利对肝癌SMMC-7721细胞增殖、凋亡的干预作用

目的 :研究尼美舒利对肝癌SMMC 772 1细胞增殖、凋亡的干预作用。方法 :研究对象为SMMC 772 1肝癌细胞系 ,细胞增殖采用MTT比色法检测 ,细胞凋亡采用电镜观察、流式细胞仪和TUNEL法检测。结果 :尼美舒利显著抑制体外培养的SMMC 772 1细胞增殖 ,随着药物剂量的增加和时间的延长 ,SMMC 772 1的生长、增殖明显抑制 ,呈剂量和时间依赖性。流式细胞术、TUNEL法检测示 ,使用不同浓度尼美舒利作用于SMMC 772 1细胞 72h后 ,可诱导SMMC 772 1细胞凋亡 ,随着药物浓度增加 ,凋亡率和凋亡指数均显著增加 ,差异有显著性。结论 :选择性COX 2抑制剂尼美舒利可以显著抑制SMMC 772 1细胞增殖 ,且呈明显的时间、剂量依赖关系 ,同时诱导SMMC 772 1细胞凋亡 ,且具有剂量依赖性