兔VX2肾移植瘤TACE治疗前后DWI特征与细胞密度的对照研究

目的探讨兔VX2肾移植瘤TACE治疗前后ADC值与细胞密度变化的对应关系,明确DWI在肾脏肿瘤治疗后随访的生物学基础。方法建立兔VX2肾移植瘤动物模型,并随机分为对照组和TACE组。对照组经肾动脉注射生理盐水10ml,TACE组注射超液态碘油2 mg/kg和阿霉素2 mg/kg。TACE术前、术后7 d行常规MRI及扩散加权成像检查,记录治疗前后肿瘤磁共振表现特征及表现扩散系数（ADC值）。细胞密度根据HE染色图片进行分析。结果 TACE组和对照组肿瘤细胞密度分别为（24.05±8.13）%和（36.03±3.13）%（P=0.022）。TACE术后ADC值为（1.321±0.125）×10^-3mm^2/s,高于对照组[（1.146±0.133）×10^-3mm^2/s]（P=0.004）。两组肿瘤细胞密度均与ADC值呈负相关（r对照组=-0.697,P=0.025;rTACE=-0.615,P=0.033）。结论兔肾VX2移植瘤TACE治疗后ADC值变化取决于肿瘤组织细胞密度的变化,DWI能反映肿瘤治疗前后组织微观结构的变化。     

胃癌组织中ASPP1蛋白的不同表达状态及其意义

目的 探讨胃癌组织中ASPP1蛋白表达规律及其与p53表达状态的相关性及与临床病理特征的关系.方法 采用免疫组织化学PV-6000法检测30例胃癌和15例相应癌旁组织ASPP1蛋白和p53的表达,比较ASPP1在胃癌组织与癌旁组织中表达的差异,并分析其与p53表达及临床病理特征的相关性.结果 胃癌组织中ASPP1的表达显著低于癌旁正常胃组织（P〈0.01）,ASPP1与患者的性别、年龄和浸润深度、分化程度、临床分期及淋巴结转移无关（P〉0.05）.而ASPP1的表达在p53阴性状态下,胃癌组织中ASPP1的表达显著高于癌旁正常胃组织（P〈0.05）;在p53阳性状态下,胃癌组织中ASPP1的表达与癌旁正常胃组织无明显差异（P〉0.05）.结论 在p53阴性的状态下,人类胃癌组织中ASPP1低表达,ASPP1的表达降低可能与胃癌的发生密切相关.     

Two polymorphisms in the TIM‐4 gene are associated with asthma in a Chinese Han population

The gene family of the T cell immunoglobulin and mucin domain (TIM) proteins encodes cell surface receptors that are involved in the regulation of Th1‐ and Th2‐cell‐mediated immunity. TIM‐1 gene has been found to be associated with asthma in several populations. TIM‐4, the natural ligand for TIM‐1, may influence the susceptility to asthma.To investigate the association of the TIM‐4 gene polymorphisms with asthma in a Chinese Han population. Three single nucleotide polymorphisms (SNPs) in TIM‐4 gene, rs6882076, rs12658558 and rs4702747, were genotyped in 551 unrelated asthma patients and 549 healthy controls. We found that two SNPs of the TIM‐4 gene, rs6882076 and rs4702747, were associated with asthma susceptibility in our study population (with P‐values = 0.009 and 0.005 respectively). No association was observed between asthma and rs12658558. Our results suggest that TIM‐4 gene polymorphisms are associated with asthma in a Chinese Han population.     

α-2a干扰素对大鼠肝纤维化TGF-β1表达的影响

目的 建立CCl4复合因素诱发大鼠肝纤维化模型,观察α-2a干扰素(IFNα-2a)对大鼠肝纤维化的治疗作用及对转化生长因子β1(TGF-β1)表达的影响,并探讨其治疗作用的机制.方法 49只雄性Wistar大鼠随机分为5组,除正常对照组外,其余4组均采用40% CCl4橄榄油溶液腹腔注射联合20%酒精灌胃造模.造模成...     

G-CSF联合VEGF增加APP转基因小鼠AD模型海马CD133及SYN的表达

目的 观察粒细胞集落刺激因子(G-CSF)联合血管内皮生长因子(VEGF)对APP转基因AD小鼠模型突触可塑性的影响.方法 54只10月龄APP转基因小鼠随机分为G-CSF联合VEGF干预组(联合组)、G-CSF干预组及对照组3组.联合组:腹腔注射VEGF(8 μg/kg·d)连续3 d,之后皮下注射G-CSF(50μ...     

循证护理对临床护理管理的启示

循证护理作为护理学科的新领域,对护理学科的发展起到十分重要的推动作用,可以在包括护理行政管理、查房管理、带教管理、科研管理、健康教育等多个方面为临床护理管理提供启示,作为临床护理管理者要掌握并灵活运用循证护理以促进护理学科的发展.     

Oct4在多种人腺癌组织中表达水平的研究

目的：研究5种人腺癌中Oct4的表达情况,为进一步探索其在肿瘤发生中的机制提供基础。方法：应用乳腺癌、胃癌、胰腺癌、肠癌、子宫内膜癌5种腺癌的组织芯片,通过免疫组织化学染色研究Oct4蛋白在组织中的表达情况,并与癌旁正常组织进行比较。结果：Oct4在5种腺癌肿瘤组织内均有表达,胃癌组织内Oct4的表达高于癌旁正常组织（P〈0．05）,其他腺癌组织巾Oct4的表达与癌旁组织差异无统计学意义;在乳腺癌和肠癌中,无淋巴结转移患者的肿瘤组织中Oct4表达高于淋巴结转移患者的肿瘤组织（P〈O．05）,而在胃癌、胰腺癌及子宫内膜癌患者中未观察到这种差异;5种腺癌的肿瘤组织内,未发现Oct4的表达与临床分期有关。Oct4在癌旁正常组织中（子宫内膜癌除外）主要定位于细胞浆,而在腺癌组织中既表达于细胞核,也表达于细胞浆。结论：Oct4的表达可能是肿瘤发生过程中的早期事件,不同腺癌组织中Oct4的表达情况不完全相同,Oct4的核／浆表达模式可能与其在肿瘤发生中的作用有关。     

不同剂量骨髓间充质干细胞对大鼠急性肺损伤的影响

目的探讨骨髓间充质干细胞(MSC)对大鼠急性肺损伤的作用及机制。方法雄性Wister大鼠4只,用于骨髓间充质干细胞采集;分离培养大鼠骨髓间充质干细胞,传致第Ⅳ代备用。雌性Wister大鼠64只,随机分为对照组(C组)、内毒素肺损伤组(LPS组)、骨髓间充质干细胞治疗大剂量组(MSCH组)和小剂量组(MSCL组)。C组和肺损伤模型经气管内分别注入生理盐水和内毒素,1小时后,C组和LPS组大鼠经尾静脉注入生理盐水,MSC组(MSCH组和MSCL组)大鼠经尾静脉注入骨髓间充质干细胞混悬液2 mL(1×106和1×105两个剂量)。分别在24 h和72 h留取标本,检测肺组织形态学、肺组织湿干重比、支气管肺泡灌洗液(BALF)白细胞总数、蛋白含量、肿瘤坏死因子-α(TNF-α)和白细胞介素-8(IL-8)水平。结果 LPS组和C组比较,肺组织病理评分、湿干重比、BALF中白细胞总数、蛋白含量、TNF-α及IL-8水平显著升高;MSC两组较LPS组各炎症指标均有明显改善,且MSCH组较MSCL组效果显著。结论 MSC移植可显著减轻内毒素诱导的急性肺损伤,且这种作用强度是剂量相关的。     

Expression of peptide fragments from proADM and involvement of mitogen‐activated protein kinase signaling pathways in pulmonary remodeling induced by high pulmonary blood flow

Pulmonary arterial hypertension (PAH) is a life‐threatening disease characterized by progressive pulmonary arterial remodeling and right ventricular failure. Despite recent advances in pathophysiological mechanism exploration and new therapeutic approaches, PAH remains a challenging condition. In this study, we investigated the roles of the peptide fragments from proadrenomedullin (proADM) such as adrenomedullin (ADM), adrenotensin (ADT), and proadrenomedullin N‐terminal 20 peptide (PAMP) during pulmonary remodeling caused by high pulmonary blood flow, and probed the possible involvement of mitogen‐activated protein kinase (MAPK) signal transduction pathways. Sixteen rat models of PAH were artificially established by surgically connecting the left common carotid artery to the external jugular vein. We subcutaneously injected an extracellular signal‐regulated protein kinase (ERK1/2) inhibitor, PD98059, in eight rats, treated another eight rats with an equal volume of saline. Eight rats without connections served as the control group. We observed that mRNA expression levels of ADM, stress‐activated protein kinase (SAPK), and ERK1/2 were significantly elevated in the shunted rats; furthermore, ERK1/2 levels were significantly inhibited by PD98059. Protein levels of ADM, PAMP, p‐SAPK, and p‐ERK1/2 were significantly higher ADT was lower, and p‐p38 remained unchanged in the rat models compared with the controls. However, the protein expression of both ADM and p‐ERK1/2 was significantly inhibited by PD98059. Our results suggest that levels of ADM, ADT, and PAMP respond to pulmonary remodeling, and that activation of the SAPK and ERK1/2 signaling pathways is involved in pulmonary hypertension and artery remodeling caused by high pulmonary blood flow.     

中药清热利湿饮对HaCaT细胞周期的影响

目的 探讨中药清热利湿饮水提液对经肿瘤坏死因子α（TNF-α）诱导活化的人永生化角质形成细胞（HaCaT）细胞周期的影响.方法 采用MTT法检测TNF-α对HaCaT细胞增殖的影响,流式细胞仪技术检测清热利湿饮提取液对HaCaT细胞周期的影响.结果 经2.5×10^5 u/L的TNF-α诱导的HaCaT细胞增殖率比对照组增加了（14.11±2.97）％,12.5g/L和15 g/L清热利湿饮可使HaCaT细胞阻滞于S期,与TNF-α对照组相比,S期比率分别增加了10.63％和21.55％.结论 TNF-α可使HaCaT细胞活化及过度增殖,一定浓度的清热利湿饮以浓度依赖的方式干扰正常的HaCaT细胞周期,通过阻滞细胞的周期时相而抑制HaCaT细胞的分裂和过度增殖.