支气管肺泡灌洗治疗老年肺部感染疗效观察

目的:探讨支气管肺泡灌洗(BAL)治疗老年肺部感染的临床疗效.方法:将80例老年肺部感染患者随机分为治疗组40例和对照组40例.2组在全身应用抗生素、营养支持、纠正水电解质等治疗基础上,对照组给予常规吸痰治疗及体位引流排痰,治疗组每周行BAL治疗1～2次,比较2组临床表现和影像学变化以评价肺部感染控制情况.结果:治疗后...     

耳甲电针对冷应激大鼠脂蛋白代谢的调节作用

ObjectiveTo explore whether auricular concha eletroacupuncture (ACEA) is effective in regulating lipid-lipoprotein metabolism in rats submitted to cold stress.MethodsThirty-six adult male Sprague–Dawley rats were placed in four groups(9 rats in each group), the rats in three groups of which were submitted to cold stress for fourteen days, the last one of which was a control group. After the cold stress process, in those three groups, the rats of one group were with no treatment (stress only), two were treated with either ACEA or auricular margin eletroacupuncture (AMEA) repeated for fourteen days. On the 14th day, all the rats were sacrificed after all experimental procedure for blood sampling. Blood glucose, triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) levels were tested by using the collected serum. Plasma lecithin-cholesterol acyltransferase (LCAT) was measured with ELISA kit.ResultsACEA down-regulated the TG level (P < 0.05) and LDL-C level (P < 0.05), and up-regulated HDL-C level (P < 0.05) and LACT level (P < 0.05). AMEA did not regulate the bio-markers.ConclusionACEA played an important role in regulating lipid-lipoprotein metabolism in rats submitted to cold stress.     

Psychophysiological Effects of Bamboo Piants on Adults

The present study was conducted to clarify the psychophysiological relaxation effects of viewing bamboo on university students. Forty healthy Chinese participants enrolled in this study to clarify the psychophysiological relaxation effects of viewing bamboo. The effects of visual stimulation using a pot both with and without a bamboo were recorded by measuring the student's blood pressure, EEG and STAI. We observed that viewing bamboo plants resulted in significantly lower systolic(female, P <0.001; male, P < 0.001;P < 0.05) and diastolic (female, P < 0.001; male, P < 0.001;P < 0.05) blood pressures, but no changes in the pulse rate (female, P = 0.09; male, P = 0.07;P > 0.05)were observed. The results of the EEG analysis indicated brainwave variation (all P < 0.05) and lower anxiety scores (P <0.01) after 3 min of viewing bamboo compared with the control. These findings indicate that visual stimulation with bamboo plants induced psychophysiological relaxation effects on adults.     

同位素标记相对与绝对定量技术筛选出Vinculin蛋白在进展期胃癌组织中低表达及其预后意义

 目的 应用同位素标记相对与绝对定量蛋白组学（isobaric tags for relative and absolute quantification,iTRAQ）技术鉴定和筛选进展期胃癌复发转移相关的差异蛋白;检测筛选出的目标蛋白Vinculin表达水平及其预后意义。方法 应用iTRAQ技术在正常胃黏膜组织组、胃癌复发组和胃癌未复发组,检测差异表达的蛋白。然后选取差异性表达显著的Vinculin蛋白,采用免疫组织化学（immunohistochemistry,IHC）技术在110例人胃癌组织标本中检测其表达情况,并统计分析Vinculin蛋白表达量与临床病理学指标的关系;采用Kaplan-Meier生存曲线分析,探究Vinculin蛋白表达与胃癌患者生存期的关系。结果 Vinculin蛋白主要表达于细胞质中近胞膜处;在110例人胃癌组织标本中,Vinculin蛋白阳性表达46例（41.8%）,缺失表达64例（58.2%）。统计结果显示,Vinculin蛋白缺失表达与淋巴结转移（P=0.018）、肿瘤TNM分期（P=0.022）、血管侵袭（P=0.002）显著正相关。Kaplan-Meier生存曲线分析结果显示,Vinculin缺失表达与胃癌患者不良预后正相关（P=0.011）。结论 Vinculin蛋白缺失表达与肿瘤转移和预后不良相关,提示其表达可以作为预测胃癌侵袭转移的潜在指标。     

针灸治疗慢性萎缩性胃炎临床随机对照试验研究综述＊

目的 评述近20年来针灸治疗慢性萎缩性胃炎临床随机对照试验研究的现状。方法 检索Medline、Embase、Cochrane图书馆、中国知网数据库、重庆维普中文科技期刊数据库、万方知识服务平台数据库、中国生物医学文献数据库。检索时间从1997年1月1日截止至2017年12月31日。纳入以针灸疗法为主要干预手段治疗慢性萎缩性胃炎的随机对照试验。对检索到的文献进行筛选、改良的Jadad质量评分、数据信息提取与数据库的建立,提取的数据信息主要包括文献的发表数量、诊断标准、治疗方法与结果、腧穴选择与归经、治疗时间与疗程、疗效判定标准、结局指标等。结果 针灸治疗慢性萎缩性胃炎的随机对照试验的文章呈现逐年增多的趋势,诊断标准和疗效标准的使用缺乏统一和规范。治疗方法主要有两类,一类为针灸疗法配合药物治疗,一类为各种针灸疗法的单独或组合运用。单独作为干预措施使用的针灸疗法以针刺配合灸法最多见。取穴组方具有远近配穴、俞募配穴及循经配穴的特点,使用频率高的腧穴依次为足三里、中脘、胃俞、脾俞、内关等。选取的经脉以足阳明胃经、任脉、足太阴脾经及足太阳膀胱经居多;治疗疗程的各临床试验差异较大,结局指标以有效率为最多。临床试验的方法学质量普遍偏低。结论 目前针灸治疗慢性萎缩性胃炎疗效显著,但临床方案设计的诸多环节尚有欠缺,影响临床研究的可重复性和临床结论的准确性,有待在今后的临床研究中加以改进和完善。     

性病/艾滋病图片展览参观前后知识、态度、行为变化分析

目的：了解本地区艾滋病知识（Ｋ）、态度（Ａ）,行为（Ｐ）以及性病／艾滋病图片展览的效果。方法：采用自己设计的调查问卷对参观者进行调查。结果：本地区艾滋病知识知晓率为６３．４％。知识掌握情况;机关人员〉本市居民〉高中学生〉外省市人员。     

Observation of the Clinical Effects of DING＇s Rolling Technique for Acute Lumbar Sprains

目的：观察丁氏（扌衮）法为主的推拿手法治疗急性腰扭伤的临床疗效。方法：将243例急性腰扭伤患者随机分为2组,治疗组131例,采用丁氏（扌衮）法进行推拿治疗;对照组112例,采用针刺后溪穴结合常规推拿手法治疗。结果：治疗组治愈98例,好转33例,无效0例;对照组治愈80例,好转29例,无效3例,两组有效率无统计学差异。结论：以丁氏（扌衮）法为主的推拿手法对急性腰扭伤疗效显著,且操作方便。     

PD-1/PD-L1 antagonists in gastric cancer: Current studies and perspectives

Immune checkpoints release suppressive signals for T cells, which enable the tumors to escape from immune destruction and provide a new concept that uses the capabilities of the immune system as a therapeutic target for tumors. At present, programmed death receptor 1 (PD-1)/programmed death ligand-1 (PD-L1) has become the most promising therapeutic target. PD-1/PD-L1 blockades exhibit long-lasting antitumor efficacy and safety in patients with various cancers, such as melanoma and non-small-cell lung cancer. Moreover, PD-L1 is highly expressed in the peripheral blood and tumor specimens of patients with cancer, and the expression of PD-L1 is positively correlated with various pathological features and may serve as a predictor of poor prognosis or a diagnostic tool. Clinical trials have verified that PD-1/PD-L1 blockade therapy benefits patients with advanced gastric cancer or gastroesophageal junction cancer. Furthermore, there are many molecules involved in the regulation of PD-1/PD-L1 expression, and the modification of these molecules via drugs and combinations with PD-1/PD-L1 inhibitors may further improve the efficacy of immunotherapy for gastric cancer. In this review, the efficacy, safety, and possible combination treatment options of PD-1/PD-L1 in gastric cancer are reviewed in experimental and clinical settings.     

过表达miR-520d 通过抑制自噬蛋白Beclin1 逆转三阴性乳腺癌细胞化疗耐药性

[摘要] 目的：探讨miR-520d 通过调控自噬逆转三阴性乳腺癌（TNBC）细胞化疗耐药的作用及分子机制。方法：以人TNBC细胞系MDA-MB-231 和MDA-MB-468 为亲本株细胞构建多西他赛（Doc）耐药细胞株MDA-MB-231/Doc 和MDA-MB-468/Doc,实验分为空白组（亲本细胞）、对照组（耐药细胞组）和过表达miR-520d 组。用qPCR检测空白组和耐药细胞组细胞中miR-520d 的表达水平,MTT实验检测过表达miR-520d 的耐药细胞对Doc 的敏感性,MDC染色后荧光显微镜观察细胞中自噬小体的发生情况、共聚焦显微镜观察过表达miR-520d 的耐药细胞中自噬相关蛋白LC3 阳性的细胞数。用荧光素酶报告基因实验验证miR-520d 与Beclin1 的靶向关系。用WB实验检测过表达miR-520 对细胞中自噬相关蛋白Beclin1 和LC3Ⅰ、LC3Ⅱ表达的影响。结果：TNBC耐药细胞中miR-520d 的表达水平明显低于空白组细胞（P<0.01）。过表达miR-520d 的耐药细胞对Doc的敏感性显著提高（P<0.01）、细胞的自噬活性明显降低（P<0.01）。荧光素酶报告基因实验证明Beclin1 是miR-520d 可能的靶分子。Doc 与miR-520d mimics 联合使用可降低TNBC耐药细胞中LC3-Ⅱ/Ⅰ比值和自噬蛋白Beclin1 的表达水平（均P<0.05）。结论：通过调控miR-520d 水平可能改变自噬蛋白Beclin1 表达,从而逆转TNBC细胞Doc化疗耐药性。