《中华医院感染学杂志》被引分析研究

目的从文献引证的角度,分析1995-2004年《中华医院感染学杂志》连续10年的文献计量指标,动态全面地了解该刊的学术质量和水平。方法利用解放军医学图书馆研制的《中国生物医学期刊引文数据库》,对《中华医院感染学杂志》被引文献进行计量分析和评价。结果各年总被引频次、即年指标和影响因子等指标均呈逐年上升趋势;论文作者分布广泛,但主要集中在北京、广东、上海等经济较发达的省和重点医药院校的附属医院和特大型医院。结论《中华医院感染学杂志》所载论文质量较高,能全面反映我国医院感染学领域的研究成果和最新研究进展,是我国医学期刊乃至整个科技期刊领域重要的核心期刊。     

Effect of taurine on GFAP and TauT expressions in rat retinal Müller cells in high glucose culture

Objective： To detect the expression of glial fibrillary acid protein （GFAP） and taurine transporter （TauT） in the retinal Müller cells in high glucose culture with taurine and to explore the influence of glucose on the taurine transporting, and the possible protective effects of taurine on MUller cells in early diabetic retinopathy. Methods： The Müller cells from the rat retina were cultured in high glucose, and GFAP and Taut expressions were detected in the cells treated with different doses of taurine by immuocytochemical fluorescein staining and Western blotting. Results： High glucose enhanced the expression of GFAP and decreased the expression of TauT in Müller cells. Taurine decreased the up-regulation of GFAP in the cells which was induced by high glucose; 0. 1-10 mmol/L taurine increased the expression of TauT in Müller cells. Conclusion： Taurine can inhibit the changes in Müller cell resulted from high glucose.     

Mandibular Hader bar attachment overdenture wearers: A five-year longitudinal clinical evaluation by electromyographic analysis

IntroductionElectromyography is used to evaluate the muscle activity of temporalis and masseter muscles in a comparative clinical research on effectiveness of masticatory cycle by conventional overdenture and use of overdenture with Hader bar attachment for five years established on a scientific level and is first of its kind.AimThe purpose of this study is to determine the response of co-ordination of stomatognathic system and the functional status of long-term overdenture use, which can be recorded in EMG.Material and MethodsTen patients were treated with maxillary conventional complete denture and mandibular overdenture. Electrical activity of masseter and temporal muscles were obtained in 3 groups. (Gp. I) After insertion of conventional overdenture with copings; (Gp. II) Overdenture with Hader bar attachment and (Gp.III) five years of use of overdenture with Hader bar attachment.Results and conclusionThe mean and standard deviation for all the patients showed an increase in muscular activity of temporalis and masseter muscles after long-term rehabilitation (x = 0.405; 0.407 and s = 0.0668; 0.1344 respectively, p = 0.0042 and 0.0074 which is <0.05). This study concludes that overdenture with Hader bar system, after five years of function provide a sound justification for a viable alternative treatment modality to provide overdenture with attachment service to patients.     

骨髓源性干细胞的异质性及在肠道修复中的作用研究进展

目的 对骨髓源性干细胞(bone marrow derived stem cells,BMDSCs)的异质性及其形成机制和意义进行综述,分析其在肠上皮重建中的作用和可能机制.方法 查阅和分析近年有关BMDSCs异质性和其在肠上皮损伤修复中作用的相关文献,并作进一步综合分析.结果 BMDSCs异质性可以解释其多向分化能力,可能以直接定植于肠道或通过和肠道干细胞融合以及促进损伤微环境的重建等多种机制参与肠上皮功能修复.结论 BMDSCs在炎症性肠病等胃肠道损伤和修复中具有广阔的应用前景.     

高血脂对大鼠血液流变特性和心肌肌钙蛋白的影响

目的 观察高血脂大鼠早期血液流变特性的变化特点,探讨心肌缺血性损伤的发生机制.方法 给予72只SD大鼠高胆固醇+猪油+猪胆盐饲喂,建立高血脂大鼠模型即高脂组(HC),与普通饲料饲喂的36只SD大鼠即正常对照组(NC)相比较,饲喂10-90 d,每间隔10天为一取样时相点,测定9组高血脂大鼠的血脂、心肌肌钙蛋白Ⅰ(cTnI)和血液流变特性指标,并观察心肌及冠脉血管的病理变化.结果 本实验模型主要表现为高胆固醇、高低密度脂蛋白血症(LDL),随高脂饲喂时间延长,血浆粘度逐渐升高,红细胞变形性下降.饲喂50 d后cTnI升高,病理提示大鼠心肌出现局灶性缺血性损伤,相关分析表明高血脂大鼠心肌缺血性损伤的发生与血高胆固醇、高LDL密切相关.结论 高胆固醇血症血液流变特性的异常改变,是早期心肌缺血性损伤发生的原因之一.     

放烧复合伤大鼠血清对小鼠骨髓造血祖细胞的影响

以单纯烧伤、放射损伤为对照 ,观察放烧复合伤大鼠血清对骨髓造血祖细胞的影响及细胞因子的变化。用6 0 Coγ射线全身照射 12Gy ,5kW溴钨灯致大鼠背部 30 %全身体表面积Ⅲ度烧伤。伤后 3、12、2 4、4 8、72和 96小时无菌抽取大鼠血清。按 10 μg m1蛋白加入到骨髓红系或粒系培养体系中培养。伤后 2 4小时 ,应用ELISA法检测血清TNFα水平 ,应用放射免疫法检测IL 6含量。结果显示 ,无论烧伤或放烧复合伤血清组 ,伤后 3、12、2 4、4 8、72和 96小时的CFU E、BFU E和CFU GM培养集落数 ,均显著高于正常对照组 (P <0 .0 1) ,且以伤后 2 4小时最高 ,烧伤组高达 342 .8%、2 6 1.6 %和 2 2 8.4 % ;复合伤组为 2 5 2 .4 %、2 0 5 .1%和 174 .2 %。但当加入放射损伤大鼠血清时 ,CFU E、BFU -E和CFU GM集落数生长较少 ,最高为 12 .7%。同时 ,烧伤组和复合伤组血清TNFα和IL 6水平显著高于正常对照组 ,更高于放射损伤组。结论 :烧伤或放烧复合伤后的血清对骨髓红系和粒系造血均有明显的促进作用 ,放射损伤后的血清则有明显的抑制作用 ;该作用与血清细胞因子变化有关。     

Evaluation of periodontitis in hospital outpatients with major depressive disorder. A focus on gingival and circulating cytokines

An imbalance in stimulated cytokine production is associated with the etiopathogenesis of numerous diseases such as major depressive disorder (MDD) and periodontal disease. Increased cytokine levels have been reported in the gingival crevicular fluid (GCF) of patients with MDD. Thirty-six outpatients with MDD participated in this study. Each outpatient was age-matched (±3 years) with a healthy control (n = 36). The patients were controlled for race and smoking habits. Unstimulated and stimulated interleukin 6 (IL-6), interleukin 1β (IL-1β), and interferon-γ (INF-γ) production in whole blood culture (WBC) and IL-6 and IL-1β levels in the GCF were evaluated. Circulating levels of IL-6 and IL-1β (unstimulated) as well as GCF IL-1β were modestly lower in MDD patients, compared to the levels in age-matched controls (Mann–Whitney, p = 0.002, 0.0075, ANCOVA, p = 0.025, respectively). In the unstimulated group, there was no correlation between the levels of circulating IL-6 and GCF IL-6 (r = 0.07, p = 0.67), and between the levels of circulating IL-1β and the IL-1β level in the CGF (r = −0.08, p = 0.63). In the LPS stimulation group, there was no correlation between the levels of circulating levels of IL-6 and GCF IL-6 (r = 0. 02, p = 0.91) or between the circulating IL-1β and GCF IL-1β (r = 0.13, p = 0.42). We observed modest immunosuppression in MDD patients (evaluated by no stimulation whole blood culture [WBC]), especially in patients with melancholic depression, chronic depression, and severe depression.     

Müller细胞与糖尿病视网膜病变

视网膜中含有两种主要的大胶质细胞，即Müller细胞和星形胶质细胞，而Müller细胞是视网膜中的主要胶质细胞。近年来研究发现，糖尿病时视网膜发生微血管病变之前就出现了视网膜Müller细胞功能的改变，包括谷氨酸代谢异常、胶质细胞反应性增加、Müller细胞凋亡等，这些改变统称视网膜神经变性。目前越来越多的研究集中在Müller细胞上，不仅因为Müller细胞的改变发生在糖尿病早期，而且Müller细胞的改变可能是引起随后视网膜微血管病变的一个主要原因。本文主要综述糖尿病早期Müller细胞的改变及研究Müller细胞的意义。     

Sedentary Behavior,Physical Activity,and All-Cause Mortality: Dose-Response and Intensity Weighted Time-Use Meta-analysis

ObjectivesPrevious studies have placed those with excessive sedentary behavior at increased risk of all-cause mortality. There is evidence of interdependency of sedentary behavior with physical activity, and its elucidation will have implications for guidelines and practice. This study investigated if sedentary behavior–related mortality risk can be offset by moderate- to vigorous-intensity physical activity (MVPA) considered in a time-use fashion.DesignPubMed was searched (from its inception till May 2018) for studies or meta-analyses that used data harmonized for MVPA. Of the 17 data-custodians located, 7 provided data on sitting time or TV viewing time, or both. A dose-response meta-analysis modeling log relative risks of all-cause mortality against uncompensated sedentary behavior metabolic equivalent hours (USMh) was run using the robust error meta-regression method. (Registration: CRD42017062439)SettingIndividual subject data held by data custodians on this topic.ParticipantsGeneral adults.MeasurementsSedentary time, MVPA.ResultsFive harmonized cohorts of sitting time (258,688 participants) and 4 of TV viewing time (156,593 participants) demonstrated that sedentary behavior was significantly associated with mortality, but this risk was attenuated with increasing energy expenditure through MVPA modeled in a time-use fashion. The average increment in mortality per USMh spent on sitting was 1% [relative risk (RR) 1.01, 95% confidence interval (CI) 1.00, 1.02; P = .01] and that per USMh spent on TV viewing was 7% (RR 1.07, 95% CI 1.04, 1.10; P < .001). The thresholds for risk started at 7 USMh for sitting and 3 USMh for TV viewing.Conclusions/ImplicationsOur findings suggest that overall daily sitting time energy expenditure of 7 MET-hours (or TV viewing of 3 MET-hours) in excess of that expended on MVPA is independently related to all-cause mortality. These findings support the view that sitting is strongly influenced by consideration of concurrent MVPA in its impact on adverse health consequences and that the USMh is a more practical metric of sedentary behavior.