癌源exosomes的制备及其对胃癌细胞生长的作用

目的:分离癌细胞源exosomes,对其超微结构进行鉴定,探讨exosomes对胃癌细胞增值活性的影响。方法:采用超速梯度离心法从MGC 803胃癌细胞培养上清液中提取exosomes,通过透射电镜技术对其超微结构进行鉴定,并利用MTT法探讨exosomes对胃癌细胞增殖活性的作用。结果:胃癌MGC 803细胞来源的exosomess具有特征性的盘状结构,由双层膜构成,直径约50~130 nm。Exosomes以剂量依赖性的方式促进MGC 803细胞的增殖。结论:癌细胞来源的exosomes能促进胃癌细胞增殖,其表面所负载肿瘤抗原可能在肿瘤细胞增殖过程中起重要的信息传递作用。     

胶质瘤中CXCL12 CXCR4和CXCR7的表达及意义

目的检测胶质瘤中趋化因子配体12(CXCL12)、趋化生长因子受体4(CXCR4)和趋化生长因子受体7(CXCR7)的表达,探讨三者在不同临床特征中的表达差异。方法选择105例胶质瘤手术后存档的蜡块及临床资料为研究组,另选择80例脑出血术后边缘经病理证实为正常脑组织蜡块为对照组。应用免疫组化方法检测两组中CXCL12、CXCR4和CXCR7的表达。结果研究组中CXCL12、CXCR4和CXCR7的阳性率明显高于对照组,研究组中3种蛋白表达的阳性率与肿瘤的最大径、Ki67表达及分级密切相关。经线性相关分析显示,研究组中CXCL12和CXCR4、CXCL12与CXCR7呈正相关。结论胶质瘤中CXCL12、CXCR4与CXCR7均高表达,CXCL12分别与CXCR4、CXCR7具有协同作用,3种蛋白对肿瘤进展有重要促进作用。     

蒙药剂型改革及制剂现代化的几点思考

蒙药研究基础比较薄弱,特别是药效物质基础、药理作用机制、配伍理论等方面的研究目前仍处在起步阶段。其次,人才队伍建设滞后,尚未形成一支联合攻关与科技创新能力较强的蒙药新制剂研发团队。此外,国家和自治区在蒙药研发方面的经费投入仍相对不足,政策支持力度也不够有效。目前,蒙药剂型改革与制剂现代化研究工作面临一定的发展机遇。具体表现在,国家和自治区政府对此项工作高度重视,先后出台振兴发展蒙医药产业的一系列政策措施,研发经费投入也在逐年增加。此外,近年来自治区的一些高校先后建立了专门从事蒙医药新制剂研究的重点实验室,部分蒙药生产企业也组建了蒙药制剂研究开发中心等机构,由此为今后蒙药剂型改革与制剂现代化研究奠定了坚实基础。     

临床信息多媒体导航系统构建

介绍内蒙古医科大学临床信息多媒体导航系统的设计方法及主要功能,总结其应用效果。该系统可以有效防止护理疏忽和差错的出现,简化工作程序,提高工作效率,降低沟通成本。     

神经外科术后感染脑脊液病原菌分布及耐药变化特点

目的:调查近3年北京天坛医院神经外科术后颅内感染的脑脊液细菌分布特点和耐药变化,为神经外科合理选用抗菌药物治疗颅内感染提供依据。方法:利用医院信息系统查询2010~2012年神经外科术后颅内感染患者的脑脊液培养病原菌的种类、数量、抗菌药物药敏结果等数据,并分类分析近3年内病原菌的流行特点和药敏情况。结果:近3年脑脊液共分离培养出病原菌844株,其中革兰阳性菌639株(75.7%),主要为耐甲氧西林的葡萄球菌,对青霉素类抗生素基本耐药,只对万古霉素和利奈唑胺敏感。万古霉素耐药肠球菌由2010年的0株增长到2012年的5株;革兰阴性菌205株(24.3%),主要包括肺炎克雷伯菌,鲍曼不动杆菌,铜绿假单胞菌等。其中鲍曼不动杆菌泛耐药现象十分严重。结论:引起神经外科术后颅内感染细菌耐药越来越严重,必须严密监测细菌耐药和控制预防用药疗程和抗感染药物的滥用。     

药用植物蓝刺头的研究进展

摘 要 蓝刺头（Echinops latifolius Tausch）为菊科蓝刺头属植物,其干燥根作为中药禹州漏芦用,其干燥花絮作为蒙药蓝刺头用。本文对近几年来中药禹州漏芦和蒙药蓝刺头的研究成果进行了整理,并从化学成分、工艺研究、质量控制和药理作用四个方面对两种药材进行了比较和综述,以期为药用植物蓝刺头的进一步开发和利用提供科学依据。     

正交试验优选蒽酮 硫酸法测定蒙药“那仁满都拉”水溶性糖含量条件

摘 要 目的： 优选蒽酮 硫酸法测定蒙药“那仁满都拉”水溶性糖含量试验条件,并对蒙药“那仁满都拉”水溶性糖进行含量测定。方法: 在单因素试验基础上,对影响水溶性糖含量测定的四个因素：反应温度、反应时间、硫酸浓度,蒽酮浓度进行正交试验设计,优选最佳测定条件。结果: 蒽酮 硫酸法测定蒙药“那仁满都拉”水溶性糖含量的最佳条件为：反应温度80℃、反应时间45 min、硫酸浓度75%、蒽酮浓度0.2%。在此条件下,葡萄糖在2.040 0～32.580 0 mg·mL^-1范围内线性关系良好,r=0.999 6,平均回收率为101.88%,RSD为0.69%。三批样品含量测定的RSD均小于0.95%。结论: 用蒽酮 硫酸法测定“那仁满都拉”中的水溶性糖含量时,显色条件对定量有不容忽视的影响。     

早期康复训练联合行为学疗法治疗老年脑卒中偏瘫患者的效果分析

目的：探讨早期康复训练联合行为学疗法治疗老年脑卒中偏瘫患者的效果。方法依据随机数字表将75例老年脑卒中偏瘫患者随机分为对照组（38例）和观察组（37例）,两组均给予早期康复训练,仅观察组在此基础上加用行为学疗法;分析治疗前和治疗3、6个月后的步态能力（功能性运动量表 FAC）,下肢运动功能和平衡功能（简式 Fugl-Meyer 运动功能评定量表FMA）,步行相关指标（步速和跨步长度）,日常生活活动能力（Barthel 指数）及偏侧空间忽略情况（直线二等分、线段划消及画钟试验）。结果观察组治疗3 个月后的 FMA 评分中的运动功能、Barthel 指数均优于对照组（P ＜0．05）,且治疗 6个月后的FAC 评分、FMA 评分、步行相关指标、Barthel 指数及偏侧空间忽略情况均优于对照组（P ＜0．05）。结论早期康复训练联合行为学疗法治疗老年脑卒中偏瘫患者的效果较好,不仅可改善偏瘫步态和下肢功能,而且可改善偏侧空间忽略,可在脑卒中偏瘫患者患侧功能训练中实施。     

Analysis of clinical prognosis in patients with non-hepatic hyperammonemia

The aim of this study was to evaluate the association of non-hepatic hyperammonemia (NHH) with the prognosis of critically ill patients with NHH.According to the serum ammonia level, the patients with NHH (n = 498) were retrieved by us. The risk factors of the mortality with NHH patients were investigated by conducting univariate and multivariate logistic regression analyses. A nomogram to predict the risk of hospital mortality was constructed. Receiver operating characteristic curve (ROC) analysis was conducted to compare nomogram (ammonia into a prognostic model, P1) with the simplified acute physiology II (SAPSII) and quick sequential organ failure assessment (qSOFA).Five independent factors for the mortality in patients with NHH were identified, including age, platelets, bun, hemoglobin, and ammonia. Models P1 using ammonia showed good prediction power. The AUROC of P1 (AUROC, 0.755 [95% CI, 0.713–0.796]) was higher than that of qSOFA (AUROC, 0.500 [95% CI, 0.449–0.551]), and SAPS II (AUROC, 0.703[95% CI, 0.658–0.748]).Ammonia was an independent prognostic predictor of mortality for NHH patients. We developed a nomogram that can predict hospital mortality with patients. Nomogram had superior discriminative power to qSOFA and SAPS II, indicating that the nomogram may have clinical utility.     

The study on the clinical effectiveness and safety of traditional Chinese medicine acupoint catgut embedding guided by musculoskeletal ultrasound in the treatment of nerve root sciatica: A protocol for systematic review and meta-analysis of randomized controlled trials

Background:Nerve root sciatica (NRS) is a common orthopedic disease, which usually occurs between 20 and 40 years of age, and the incidence rate is increasing year by year and is being younger. The disease has no special effect of treatment, clinically generally taking the symptomatic treatment, such as taking short-term glucocorticoids, sedatives, analgesics, and so on. Long-term use of drugs will adversely affect the patient''s gastrointestinal tract, liver, and kidney function. The surgical treatment has a high risk of surgery, high cost, side effects, and other problems, so the choice of treatment method has always been a difficult problem in clinical and scientific research. The study shows that 90% of patients with sciatica can be cured by non-surgical treatment, so conservative therapy is often used in the treatment of sciatica, traditional Chinese medicine treatment methods in the treatment of NRS has been widely used, which has achieved good results, but there is no evidence of evidence-based medicine. Therefore, this study uses systematic evaluation to conduct the scientific evaluation of the clinical effectiveness and safety of traditional Chinese medicine acupoint catgut embedding guided by musculoskeletal ultrasound in the treatment of NRS, and provide evidence-based medical evidence support for the treatment of NRS.Methods:Using the computer to retrieve the PubMed, ScienceDirect, Web of Science, Embase, Cochrane Library, CNKI, VIP, WANFANG Database, and CBM. Using the subject words and terminology words to retrieve the Chinese-English database and retrieve a randomized controlled study on the clinical effectiveness and safety of traditional Chinese medicine acupoint catgut embedding guided by musculoskeletal ultrasound in the treatment of NRS, and the range of search time is January 1990 to January 2021. The searched literature is screened and evaluated by two researchers respectively according to the inclusion and exclusion criteria. If there is disagreement, discussing it with the third researcher to determine the final inclusion of the literature. Using the RevMan 5.3 software to conduct the meta-analysis.Results:This study will compare the effectiveness and safety of traditional Chinese medicine acupoint catgut embedding guided by musculoskeletal ultrasound in the treatment of NRS.Conclusion:The results of this study will be published in internationally influential academic journals to provide evidence-based medical evidence for the clinical effectiveness and safety of traditional Chinese medicine acupoint catgut embedding in the treatment of NRS.Ethics and dissemination:This study does not involve specific patients, and all research data comes from publicly available professional literature, so an ethics committee is not required to conduct an ethical review and approval of the study.OSF registration number:DOI 10.17605/OSF.IO/Q492E.