螺旋CT在上颌前部埋伏阻生牙定位中的临床应用

目的：探索三维CT成像和多层面重建技术（MPR）在骨内埋伏阻生牙中的应用价值。方法：对9例埋伏阻生牙患者，应用螺旋CT的图像处理功能，采用表现阴影成像法和多层面重建技术，获得单纯牙体表面图像和任意平面、任意曲面的断层像。结果：能清晰的显示上颌前部埋伏阻生牙的形态、唇侧或腭侧位置、萌出方向及与邻牙的关系。结论：螺旋CT三维成像和多层面重建技术是一种即能立体展示牙齿表面形态，又能精确判断牙齿位置的影像学技术，可作为骨内埋伏阻生牙外科处理和正畸治疗前的检查手段。     

成人骨性Ⅲ类错患者的颅面结构与颈椎姿势的相关性研究

目的　研究成人骨性Ⅲ类错患者的颅面结构与颈椎姿势的相关性。方法　对 4 5例成人骨性Ⅲ类错患者拍摄自然头位时的头颅定位侧位片。结果　成人骨性Ⅲ类错患者的SN -VEL、SN -OPT、SN -CVT与SNA、SNB、SND、SNPo存在显著负相关 ,与SN -GoGn、SN -PP、SN -OP、NSGn等变量存在显著正相关。结论　成人骨性Ⅲ类错患者的颅面结构与颈椎姿势具有相关性     

256层CT下肢静脉成像评价Klippel-Trenaunay综合征

目的探讨256层CT下肢静脉成像在Klippel-Trenaunay综合征的诊断价值。方法回顾性分析经临床和影像学检查确诊的7例KTS患者的临床及影像学资料,观察其CT下肢静脉成像的影像特征。结果本组7例患者CT下肢静脉成像均显示浅静脉曲张及患肢皮下静脉畸形,其中伴有深静脉病变4例（3例深静脉狭窄,1例深静脉闭塞）。结论多层CT下肢静脉成像是KTS有效和可靠的影像检查方法,对于指导临床治疗具有重要意义。     

军医大学本科毕业生住院医师规范化培训初探

军医大学培养的五年制临床医学专业本科毕业生分配到基层部队后,从事全科医师工作,但却未经全面、系统、规范的毕业后培训,难以胜任岗位任职需要。探索基层医师住院医师规范化培训制度,系统规范地培训分配到基层的医学生,有利于推动部队卫生事业的健康发展,是提高基层部队卫勤保障能力的重要举措。     

Secretory expression of human ScFv against keratin in <Emphasis Type="Italic">Pichia pastoris</Emphasis> and its effects on cultured keratinocytes

We have got the humanized antibody with high affinity and specificity against keratin by phage antibody library technology. To improve protein yields and get high affinity and specific anti-keratin antibody, we chose to express it in Pichia pastoris. Anti-keratin ScFv gene from plasmid p3MH/ScFv was subcloned into vector pPIC9K. After confirmed by DNA sequence analysis, the recombinant plasmid pPIC9K/ScFv was transduced into the genome of GS115 P. pastoris. Mut^s multiple insert transformants were screened by G418 and induced by 5 mL/L methanol to express soluble ScFv. After 6 days of methanol induction, anti-keratin ScFv was efficiently secreted into the medium. Western blot and ELISA assay proved the expressed protein had specific keratin-binding activity. After purification, we examined its effect on cultured keratinocytes by Cell cycle analysis, Which indicated that human ScFv against keratin 17 can inhibit the proliferation of keratinocytes by influence the synthesize of keratinocyte DNA. The successful expression of anti-keratin ScFv in P. pastoris laid a solid foundation for its further application.     

Secretory expression of human ScFv against keratin in <Emphasis Type="Italic">Pichia pastoris</Emphasis> and its effects on cultured keratinocytes

We have got the humanized antibody with high affinity and specificity against keratin by phage antibody library technology. To improve protein yields and get high affinity and specific anti-keratin antibody, we chose to express it in Pichia pastoris. Anti-keratin ScFv gene from plasmid p3MH/ScFv was subcloned into vector pPIC9K. After confirmed by DNA sequence analysis, the recombinant plasmid pPIC9K/ScFv was transduced into the genome of GS115 P. pastoris. Mut^s multiple insert transformants were screened by G418 and induced by 5 mL/L methanol to express soluble ScFv. After 6 days of methanol induction, anti-keratin ScFv was efficiently secreted into the medium. Western blot and ELISA assay proved the expressed protein had specific keratin-binding activity. After purification, we examined its effect on cultured keratinocytes by Cell cycle analysis, Which indicated that human ScFv against keratin 17 can inhibit the proliferation of keratinocytes by influence the synthesize of keratinocyte DNA. The successful expression of anti-keratin ScFv in P. pastoris laid a solid foundation for its further application.     

天然抗角蛋白单克隆抗体3B4的多反应性鉴定及其分子机制的初步探讨

目的 对天然抗角蛋白单克隆抗体3B4的多反应性进行鉴定,并探讨其分子基础。方法 用间接酶联免疫吸附实验(ELISA)和免疫组化法检测3B4与多种抗原的反应活性,分析其可变区基因与氨基酸序列。结果 3B4除与角蛋白和皮肤发生较强的反应外,尚能选择性与多种具有不同表位的抗原和不同的组织反应;同源性分析表明,其重、轻链可变区基因分别与胚系基因VH1 J558.42及VK1 am4高度同源;HCDR3区为该抗体区别于其他同源性较高的免疫球蛋白的惟一基序,主要由短的侧链氨基酸组成。结论 天然抗角蛋白单克隆抗体3B4具有多反应性,由少量突变的胚系基因编码,HCDR3区的柔韧性可能是多反应性的分子基础之一。     

肝癌患者集束电极射频治疗后T淋巴细胞亚群及sIL—2R的改变

目的：研究原发性肝癌患者集束电极（多弹头）射频治疗前后T细胞亚群及sIL-2R的变化及其临床意义。方法：采用免疫荧光染色法及ELISA双抗体夹心法,检测40例原发性肝癌患者治疗前后的,T细胞亚群及sIL-2R的改变,并与健康对照组比较,结果：肝癌组患者治疗前CD^ 3和CD^ 4降低,CD^ 8和sIL-2R增高,CD^ 4／CD^ 8比值明显下降,与对照组比较,均有显著性差异（P＜0．01）。肝癌组患者治疗后CD^ 3和CD^ 4升高,CD^ 8和sIL-2R降低,CD^ 4／CD^ 8比值增高,治疗前后比较,有非常显著性差异（P＜0．01）。结论：集束电极（多弹头）射频治疗可增强原发性肝癌患者机体免疫功能,提高其抗肿瘤能力。     

Human bone morphogenetic protein-2 gene transfer induces human mesenchymal stem cell proliferation and differentiation in vitro

To identify eukaryotie expression veetor of human bone morphogenetie protein 2 peDNA3/BMP2, verify its expression in transfected hulnan mesenchynlal stem cells(hMSCs) and the effect on hMSCs differentiation. Methods: The BMP2 gene was cloned into a eukaryotic expression veetor pcDNA3. Transfeeted the reeombinant into hMSCs by liposome, Lmmunnohistochemistry and it situ hybridization methods were used to identify the expression of BMP2 mRNA and protein; ALP and Von Kossa stains were performed to identify the BMP2 gene differentiated effect on the hMSCs. Resuits: The pcDNA3/BMP2 fragments were as large as theory. BMP2 mRNA and protein were espressed and synthesized both in 48 h and 4 weeks after transfeetion, the ALP and Ca deposit eshibitiio, which marked the osteogenie lineage of hMSCs, were enhanced and Sped. Conclusion: Transfeetion of pcDNA3/BMP2 is able to provide transient and persistent expressionin hMSCs, and promote the MSCs differentiation to osleogenic lineage.     

EP核定向毁损术对大鼠血压的影响

目的　观察SD大鼠EP核 (相当于人的苍白球Gpi部 )毁损术对大鼠血压的影响 ,明确EP核对自主神经功能的调节作用。方法　选取雄性SD大鼠 2 0只 ,体重 2 0 0～ 2 5 0 g。术前 1d在生理清醒安静状态下 ,无创测量大鼠尾动脉收缩压 ,形成自身对照组。手术采用 1%戊巴比妥腹腔麻醉大鼠后 ,将大鼠固定于大鼠脑立体定向仪上 ,按EP核位置在大鼠颅骨上开一小孔 ,按预定坐标将微量注射器插入EP核团 ,缓慢将鹅膏蕈氨酸注入核团 ,退针。同样的程序毁损对侧同一核团。术后饲养大鼠 1周 ,与术前同样的条件在生理清醒安静状态下 ,无创测量大鼠尾动脉收缩压。处死大鼠后进行脑切片染色 ,确定毁损区域位置是否位于EP核。结果　双侧EP核毁损术中 ,1只因出血死亡。术后成活的 19只大鼠状态良好 ,其中 4只脑片观察 ,EP核破坏不准确而被排除。符合要求的 15只大鼠术前尾动脉收缩压为 12 4± 5mmHg(1mmHg =0 .133kPa) ,手术后 15只大鼠尾动脉收缩压为 116± 6mmHg ,(P <0 .0 5 )。结论　通过对清醒大鼠EP核毁损术前及术后的收缩压观察 .EP核对收缩压具有一定的调节作用 ,说明它对自主神经功能有一定调节作用 ,这种调节的机制有待进一步研究