Rootstock effect on serotonin and nutritional quality of tomatoes produced under low temperature and light conditions

In this study, we explored the capacity of different tomato rootstocks to improve the nutritional value of tomatoes grown in a greenhouse during the winter–spring period under low temperature and light conditions. The results showed that relative to nongrafted plants, some grafted rootstocks either did not affect or had detrimental effects on the nutritional parameters measured. In addition, the amount of serotonin was significantly lower in tomatoes from all grafted rootstocks than in tomatoes from the nongrafted plants. Nevertheless, relative to the nongrafted plants, some grafted treatments increased total macroelements (by 1.16 times), total microelements (by 1.43 times), protein content (by 17%), free total and essential amino acids (by respectively 23% and 19%), total phenolic compounds (by 11%), vitamin C (by 13.8%), lycopene (by 48%), and also total hydrocinnamic acids (by 67%) and flavonoids (by 279%). As the grafted treatments did not increase the tomato yield, we hypothesize that these compounds accumulated in the tomatoes due to the lack of increased biomass, which prevented a dilution effect on the compounds of interest in the fruits.     

后极性白内障超声乳化术的临床观察

目的探讨后极性白内障超声乳化术的手术技巧及效果。方法回顾性分析鄂尔多斯市中心医院2015年12月至2018年12月后极性白内障行超声乳化术15例(21眼)的临床资料。探讨对后囊下浑浊的处理方式和维持前房稳定的技巧,观察术中、术后并发症及术后视力。术后随访6个月。结果所有患者均植入折叠人工晶状体,15眼后囊完整,6眼后囊破裂;15眼中环形撕囊者5眼、剪除浑浊者6眼、二期行YAG激光后囊切开者4眼。术后随访6个月,最佳矫正视力>0.5者12眼,0.1~0.5者9眼。结论后极性白内障患者行超声乳化术中选择合理处理后囊、维持前房稳定,可有效提高手术成功率,获得较好的术后视力。     

2009-2018年内蒙古自治区鄂尔多斯市猩红热流行特征分析

目的了解内蒙古自治区鄂尔多斯市猩红热发病趋势及流行特征,为防控工作提供参考依据。方法利用描述流行病学方法分析鄂尔多斯市2009—2018年猩红热疫情资料。结果2009—2018年鄂尔多斯市共报告病例3150例,年平均发病率为16.50/10万,男性发病率为女性的1.58倍。发病率呈现一定的季节性,第一个高峰出现在5—7月,第二个高峰出现在10月至次年1月。病例以3~10岁年龄组高发,占病例总数的94.16%(2966/3150)。幼托儿童、学生、散居儿童报告发病数分别占全部报告发病数的60.32%(1900例)、28.22%(889例)和10.67%(336例)。结论近10年来鄂尔多斯市猩红热报告发病率整体呈上升趋势,以春末夏初和冬季高发,男性发病率高于女性。     

蒙族慢性乙型肝炎患者NK细胞的特征与肝损伤相关性分析

目的 NK细胞在抗病毒免疫过程中发挥了重要作用,本研究观察蒙族慢性HBV感染免疫耐受期和免疫清除期患者外周血NK细胞的表型和功能特点,并密切结合患者的临床指标进行分析,以期阐明抗病毒的作用机制。方法采集免疫耐受期患者24例、免疫清除期患者21例及健康对照者25例,分离外周血单个核细胞,采用流式细胞仪测定外周血NK细胞的表型、功能,及临床指标(ALT和病毒载量)。结果与免疫耐受期患者和健康对照组比较,免疫清除期患者外周血NK细胞亚群发生显著改变,表现为CD56dimNK亚群明显下降,而CD56bright NK亚群明显上升;慢性乙型肝炎免疫清除期患者的外周血NK细胞表面的活化标志物如HLA-DR,CD38和CD69的表达显著上升,差异具有统计学意义(P<0.05)。结论蒙族慢性乙型肝炎免疫清除期患者外周血NK细胞处于高活化状态,进一步表明慢性乙型肝炎患者NK细胞的高度活化与肝损伤密切相关。     

鄂尔多斯市中心医院医院感染调查

目的调查鄂尔多斯市中心医院医院感染状况,并对感染病原菌的种类分布进行分析,为合理使用抗生素,有效地预防和控制医院感染的发生提供依据。方法采用回顾性调查方法,分别计算出医院感染率、科室和部位、医院感染分布及其科室的抗生素使用情况的统计,根据临床微生物实验室的资料,按菌株数量计算出主要病原菌的构成比。结果医院感染发生率为2.92%,医院感染例次发病率为3.18%。各科室均有医院感染发生,其中烧伤科、神经外科、ICU感染率最高,分别是15.61%、10.11%、9.38%;医院感染部位以呼吸道感染为首,其次是皮肤软组织、泌尿道,构成比分别为41.07%、25.68%、18.12%;60岁以上年龄组和<10岁的年龄组医院感染率相对较高,为6.39%和4.02%。抗生素使用率为71.3%,其中妇产科使用率最高,为96.0%;感染病原菌以革兰阴性杆菌为主,但凝固酶阴性葡萄球菌居第一位。结论医院感染率在不同科室的分布不同,以呼吸道感染最常见,抗生素使用率均高于卫生部的要求,而且感染病原菌以革兰阴性菌为主。     

十二指肠肿瘤50例外科诊断与治疗

目的探讨十二指肠肿瘤的临床诊断及外科治疗效果。方法回顾性分析2008年3月—2009年5月期间于该院就诊的十二指肠肿瘤患者50例相关资料。结果胰十二指肠切除40例,术后1、2、5年生存率分别为77.5%(31/40)、60.0%(24/40)、50.0%(20/40)。十二指肠乳头癌局部切除,胰胆管引流术5例。术后16个月死亡1例,其余均生存至今(超过3年)。十二指肠节段性切除患者2例分别于术后12个月、14个月复发死亡。结论十二指肠肿瘤患者临床症状会因部位的变化而不同,但缺乏特异性。治疗方法应当依据诊断结果进行选择,以胰头十二指肠切除为主,但术后死亡率仍较高,应当结合患者的基本情况。     

Treatment of rectovaginal fistula by magnetic compression

Introduction and hypothesis

Rectovaginal fistula (RVF) is an abnormal epithelium-lined connection between the rectum and vagina. The primary effective treatment is surgical repair, but recurrence remains a challenge. Magnetic compression anastomosis (MCA), an alternative to suturing, has been developed to generate an anastomosis between various hollow viscera. We hypothesized that the MCA approach could be used to treat RVF.

Method

We designed a novel MCA device for RVF treatment and evaluated the magnetic compression procedure in a RVF pig model in comparison with the traditional suturing procedure. Following satisfactory outcomes, we also applied the MCA procedure to a human patient with recurrent RVF. The MCA device was designed based on the anatomical characteristics of the pig vagina and previous literature. The pig RVF model were established surgically (n?=?12), and compression and control groups were each treated. The data were analyzed by one-way analysis of variance.

Results

qqExcept in one animal in each group, the RVF site was smooth and healing was complete. Histological analysis confirmed complete healing of the RVF with high histological continuity to neighboring tissues. The compression procedure applied to our patient with RVF was successful. The patient recovered quickly without complications, and RVF did not recur during a 15-month follow-up.

Conclusions

From this preliminary investigation, MCA using our novel device appears to be a safe, simple, and effective nonsurgical procedure for the treatment of RVF.

     

不同分离方法及培养条件对人脐带间充质干细胞生物活性的影响

目的:观察不同分离方法及培养条件对人脐带间充质干细胞(Human umbilical cord-mesenchymal stem cells,hUCMSCs)生物活性的影响。方法:分别用组织块贴壁法、酶消化法获取hUCMSCs,进行原代培养,记录各组首次传代时间,相差显微镜观察细胞的形态,流式细胞仪鉴定其CD29、CD44、HLA-ABC、CD34、CD45、HLA-DR抗原表达。分别用DMEM-LG、DMEM-HG和DMEM-F123种培养基培养第3代、第7代细胞,MTT法比较不同时间点3种培养基培养细胞的生长情况。结果:两种分离方法均能得到较均一的梭形或者多角形贴壁细胞,胞核大胞浆丰富,CD29、CD44、HLA-ABC抗原表达阳性,CD34、CD45、HLA-DR抗原表达阴性。用DMEM-F12培养的细胞活力更好,细胞生长更迅速。结论:两种分离方法均能得到hUCMSCs,DMEM-F12培养基更能够促进细胞的增殖,较适合于培养hUCMSCs。     

应用Orem自护理论对人工全髋关节置换术患者的护理指导

目的：观察Orem自护理论在人工全髋关节置换术患者中的应用效果。方法：将79例人工全髋关节置换术患者随机分为两组,观察组39例（实施自我护理）,对照组40例（实施常规护理）,比较两组并发症、自理能力。结果：两组患者并发症差异无统计学意义（P〉0.05）,而自理能力观察组明显高于对照组（P〈0.01）。结论：应用Orem自护理论对人工全髋关节置换术患者实施护理可提高患者自理能力,促进患者早日康复,提高了患者的生活质量。