TCRVβ7.1基因修饰T细胞对乳腺癌细胞杀伤作用的研究

目的：观察TCPVβ7．1基因转染前后正常人外周血淋巴细胞对乳腺癌细胞株杀伤活性的影响。方法：脂质体包裹PdWA3.1vβ7.1后转染健康人PBMC，流式细胞仪检测PdWA3.1Vβ7.1基因表达，改良MIT法检测TCRVβ7．1基因转染前后正常人外周血淋巴细胞对乳腺癌细胞株杀伤活性。结果：TCRVβ7．1基因转染可显著增加正常人PBMC该基因表达，转染前后正常人外周血淋巴细胞对乳腺癌细胞株杀伤活性有显著性差异。结论：用TCR基因修饰可明显提高正常人PBMC对乳腺癌细胞杀伤作用。     

Segmentation of Bacteria Image Based on Level Set Method

In biology ferment engineering, accurate statistics of the quantity of bacteria is one of the most important subjects. In this paper, the quantity of bacteria which was observed traditionally manuauy can be detected automatically. Image acquisition and processing system is designed to accomplish image preprocessing, image segmentation and statistics of the quantity of bacteria. Segmentation of bacteria images is successfully realized by means of a region-based level set method and then the quantity of bacteria is computed precisely, which plays an important role in optimizing the growth conditions of bacteria.     

Transcription Factor PU.1 Promotes Conventional Dendritic Cell Identity and Function via Induction of Transcriptional Regulator DC-SCRIPT

1. Download high-res image (168KB)
2. Download full-size image

     

Prevalence and incidence of antibodies to Borrelia burgdorferi and to tick-borne encephalitis virus in agricultural and forestry workers from Tuscany, Italy

The ticks Ixodes persulcatus and Ixodes ricinus are the main vectors of both Borrelia burgdorferi sensu lato and tick-borne encephalitis (TBE) virus in Eurasia. Borrelia burgdorferi is the cause of Lyme borreliosis, and TBE is a biphasic meningoencephalitis induced by an arbovirus belonging to the flavivirus family. The principal aims of the current investigation were (i) to determine the frequency of serological evidence of Borrelia burgdorferi sensu lato and TBE infections in healthy agricultural and forestry workers, (ii) to determine the incidence of seroconversion for antibodies against Borrelia burgdorferi sensu lato and TBE virus in Tuscan workers during a 1-year survey; and (iii) to assess the occupational risk for agricultural and forestry activities in a defined area (Tuscany, Italy). A total of 412 blood samples were taken from agricultural and forestry workers, and information on age, duration of employment, and history of tick bites was collected in a questionnaire to establish the risk factors for the diseases. Three hundred sixty-five blood donors from the same region served as controls. To estimate the rate of seroconversion, 176 of the agricultural and forestry workers were tested 1 year later. IgG and IgM antibodies against Borrelia burgdorferi sensu lato and TBE virus were detected in serum by an enzyme-linked immunosorbent assay and confirmed by Western blot analysis for Borrelia burgdorferi and by a test for inhibition of hemagglutination for TBE. Antibodies against Borrelia burgdorferi were more frequent among the workers than in the control group (7.8% vs. 4.9% in the IgG-IgM enzyme-linked immunosorbent assay and 7.03% vs. 3.56% in the confirmatory test). No seropositivity was observed for TBE virus. Eighteen of 176 subjects who underwent a second blood test developed specific antibodies against Borrelia burgdorferi within 1 year.     

Beeinflußt das geschlecht die zuweisung zur stationären psychiatrischen behandlung?

Zusammenfassung In Mannheim wurde im Jahre 1968 mit dem schrittweisen Aufbau eines gemeindenahen psychiatrischen Versorgungssystems begonnen. Bestandteil dieses Versorgungskonzepts sind Absprachen zwischen den beteiligten stationären Einrichtungen, die die Zuweisung der Patienten zur stationären Behandlung regeln. Eine modellgestützte, multivariate Analyse von Inanspruchnahmedaten einer Kohorte von 126 schizophrenen Patienten weist nach, daß die Ungleichverteilung der Geschlechter auf die Institutionen das Ergebnis dieser Absprache ist; dabei sind Krankheitsdauer und die Art der Zuweisung von entscheidender Bedeutung.Die vorliegende Arbeit entstand im Rahmen eines von der Deutschen Forschungsgemeinschaft geförderten Projekts im Sonderforschungsbereich 116 Psychiatrische Epidemiologie der Universität Heidelberg (Sprecher: Prof. Dr. Dr. M. Schmidt)     

The contribution of European case registers to research on schizophrenia

Psychiatric case registers have become important instruments for epidemiological research and for the evaluation of care provided for persons suffering from schizophrenia. Under the condition of a sufficient provision of care, case registers permit relatively reliable estimates of "treated" incidence and prevalence, and enable the investigation of associations between morbidity risks, disease courses, and variables such as ecological, social, and occupational factors. The linkage with twin, adoption, and birth registers provides new opportunities for the investigation of the impact of genetic versus environmental factors on the probability of becoming ill with schizophrenia. On the basis of long-term utilization figures, case registers enable a valid measure for controlling utilization, effectiveness, quality, and costs of care. Because case registers take demographic and regional factors into account, they enable better planning of mental health services--a task that grows more important with the shift from hospital-based to community-based treatment, especially for the chronic patients.     

中小学校学生流感疫苗接种效果评估和卫生经济学评价

目的评估中小学校学生流感疫苗接种效果,开展疫苗接种的卫生经济学评价,为制定流感疫苗接种策略提供依据。方法采用易感者-潜伏者-显性感染者/隐性感染者-移出者/恢复者(susceptible-exposed-infectious/asymptomatic-removed/recovered,SEIARR)动力学模型对既往疫情数据进行模拟,计算累计罹患率评估疫苗接种效果,计算成本-效果和成本-效益进行卫生经济学评价。结果当流感疫苗接种率为0%、50%、70%和90%时,疫情平均累积罹患率为99.79%(99.75%,99.82%)、70.84%(70.58%,71.09%)、56.81%(56.17%,57.45%)和34.29%(32.46%,36.20%),流感疫苗接种率越高,累计罹患率越低;当疫苗接种率为50%、70%和90%时,疫苗的成本-效果比为:141.03(139.86,142.19)元、133.16(131.34,135.08)元和112.90(110.03,116.11)元,疫苗的成本-效益比为:6.27(6.22,6.32)元、6.65(6.56,6.74)元和7.88(7.66,8.09)元;流感疫苗接种率越高,疫苗的成本-效果越好,成本-效益越高。结论接种流感疫苗能有效降低疫情的累计罹患率,提高接种率能有效增加疫苗接种的卫生经济学效应。     

墨玉县维吾尔族2型糖尿病合并冠心病患病现状及影响因素分析

目的 了解新疆墨玉县维吾尔族2型糖尿病合并冠心病的患病现状,为当地人群慢性病的危险因素控制和健康管理提供参考依据。 方法 选取2019年新疆墨玉县维吾尔族成人全民健康体检数据中的2型糖尿病患者为调查对象。采用多因素Logistic回归模型分析2型糖尿病合并冠心病的影响因素。 结果 2019年墨玉县维吾尔族2型糖尿病患者共14 659例,患病率为4.96%;2型糖尿病患者中,合并冠心病患者共767例,患病率为5.23%。经多因素logistic回归分析显示,女性(OR=1.218,95%CI:1.042~1.424)、年龄[40~59岁(OR=3.254,95%CI:2.151~4.922);≥60岁(OR=4.382,95%CI:2.860~6.712)]、婚姻状况[已婚(OR=12.798,95%CI:1.772~92.415);离婚/其他(OR=16.659,95%CI:2.276~121.948)]、有冠心病家族史(OR=3.878,95%CI:2.773~5.423)、高血压(OR=3.295,95%CI:2.698~4.024)、肥胖(OR=1.264,95%CI:1.084~1.475)、嗜糖(OR=1.995,95%CI:1.490~2.670)、嗜油(OR=2.406,95%CI:2.025~2.860)、嗜盐(OR=1.447,95%CI:1.151~1.820)是2型糖尿病合并冠心病的影响因素。 结论 维吾尔族2型糖尿病合并冠心病的危险因素较多,建议当地医疗卫生部门应重视2型糖尿病、冠心病的防治,提高居民健康意识,改变居民不良饮食习惯和生活方式,降低2型糖尿病合并冠心病的患病风险。     

饮酒与慢性阻塞性肺疾病风险关系的meta分析

目的 探讨饮酒与慢性阻塞性肺疾病风险(chronic obstructive pulmonary disease, COPD)的关系。 方法 计算机检索PubMed、Web of Science、Embase、中国知网、万方数据库和维普数据库,搜集各数据库建库至2021年11月公开发表的有关饮酒与COPD风险的观察性研究。由2位研究者根据文献纳入及排除标准独立筛选文献并提取数据。采用Stata 12.0软件进行统计学分析。 结果 共纳入8篇文献(2篇队列研究文献和6篇横断面研究文献),共纳入209 489例研究对象。meta分析结果显示,与最低饮酒量组相比,最高饮酒量组发生COPD的风险无显著变化(RR=0.97, 95%CI:0.87~1.08);与不饮酒组相比,少量或中度饮酒组发生COPD的风险降低(RR=0.87, 95%CI:0.81~0.94);与少量或中度饮酒组相比,过量饮酒组发生COPD的风险无显著变化(RR=1.26, 95%CI:0.98~1.63)。 结论 meta分析结果提示少量或中度饮酒可降低COPD的风险。过量饮酒是否会增加COPD的风险尚需进一步研究。     

2005-2019年理塘县法定传染病疫情分析

目的了解理塘县近15年法定传染病疫情形势及流行特征,为政府制定防控措施提供科学依据。方法对2005—2019年理塘县法定传染病疫情进行描述性流行病学分析。采用ArcGIS 10.3软件绘制各乡镇发病情况分布图,SPSS 21.0软件进行χ²检验、趋势性χ²检验。结果2005—2019年理塘县共计报告甲乙丙类传染病21种6154例,年均发病率为644.33/10万,发病呈上升趋势。死亡37例,年均死亡率3.87/10万,病死率0.60%。呼吸道传染病发病最高(341.12/10万);发病前3位的病种为肺结核(271.91/10万)、乙肝(103.24/10万)及包虫病(67.22/10万);肺结核、其他感染性腹泻病、艾滋病/HIV、梅毒发病呈上升趋势。3月、9月分别出现1次发病高峰;20~29岁、30~39岁和10~19岁组发病居前3位;男女性别比为1.24∶1;发病以农民、牧民及学生为主。结论2005-2019年理塘县法定传染病发病率较高且呈上升趋势,应针对高发传染病、上升趋势明显的传染病、重点人群进行分析研究,采取针对性措施控制疫情。