抗人生长激素免疫血清的制备和鉴定

Using small dosage of human growth hormone to immunize rabbit or guinea pig, it is able to induce anti-hGH antibody formation with high titre and high affinity that could be applied to hGH RIA. In the present study five rabbits and three guinea pigs were immunized with 125-200 micrograms and 250-285 micrograms per animal of hGH respectively, followed by boosters of 10-20 or 160-250 micrograms of hGH at 2-4 week intervals for 6 or 3 months. Blood was drown 1-2 weeks before each booster for determination of antibody formation. Antibody titre and affinity were successively observed and specificity of antibody was determined for the final bleeding. It was shown that titres of immune sera from guinea pigs were much higher than those of rabbit immune sera, but vice versa for antibody affinity. This might be due to larger immunogen dose used for guinea pigs than for rabbits. Fourteen different peptide hormones were tested in reference to cross-immunoreactivity to anti-hGH antibody. It could be demonstrated that the major cross-reactive hormones are hFSH and hLH, and hTSH also reacts to rabbit anti-hGH immune sera at a lesser degree. These cross reactivities are obviously owing to the molecular homogeneities between hGH and these hormones especially of their alpha-subunits.     

手法整复治疗肱骨外科颈骨折并肩关节脱位10例

<正> 肱骨外科颈骨折并肩关节脱位,是一种复杂而严重的创伤,临床上并不多见。其手法整复难度大,目前大多数人主张手术治疗,但术后易并发肩关节功能障碍,如处理不当,可造成严重致残。我科自1985～1990年共收治该病10例,均经闭合手法整复,小夹板外固定治疗,疗效满意。1 一般资料本组共10例,男3例,女7例。年龄最小7岁,最大70岁,平均51岁。病史最短3小时,最长15天,平均6.5天。左侧7例,右侧3例。     

带血管蒂岛状筋膜瓣移植治疗晚期类风湿性髋关节炎

目的：阐明带血管蒂岛状筋膜瓣移植治疗晚期类风湿性髋关节炎的疗效。材料和方法：对４２例４８髋晚期类风湿性髋关节炎病人采用以旋股外侧血管降支为蒂的股前外侧筋膜瓣移植行患髋成形术。结果：术后随访４～１０．５年，平均４．５年，髋关节活动范围术前平均１６．５°，术后２２８．６°，按照Ｃｈａｒｎｌｅｙ和吴之康人工髋关节置换术后的疗效评定标准，优良２９髋、很好１３髋、好６髋，尚可、差、很差无。结论：患者年龄在１５～４０岁之间为合适的治疗年龄。与人工髋关节置换术相比，本方法对髋关节正常结构破坏小；一旦手术失败仍可选用其它治疗方法弥补；且并发症少、费用低、远期效果好。     

SPECT对儿童癫痫的临床意义

1994年以来我院儿科用单光子发射计算机断层（SPECT）脑血流灌注显像对73例癫病患儿的结果进行了统计，并作出临床评价。临床资料一、病例资料73例中男42例，女31例。年龄2～6a26例，0～12a47例。大发作56例，小发作8例，头痛性癫痛、腹型癫病各2例，情景性癫病4例，眩晕性癫痛     

Epidemiological and pathogenic study on the outbreak of toxic shock syndrome and meningocephalitis caused by swine streptococcus

Objective: To reveal the relationship between the biological characteristics of pathogen and the pig-to-human spread of the epidemic and infectious disease in 1998 in East China. Methods: Epidemiological survey, pathological examination of pigs and patients, and pathogen isolation were performed. Results: The disease had a character of quick onset, serious symptoms, short course and high mortality. The clinical manifestations and pathological changes of the disease were high fever, sometimes with nausea, vomiting and diarrhea, then might develop to myositis, fascitis, DIC, multiple organ failure, shock and usually died in 2-3 d. Among 25 patients, 16 manifested clinically as streptococcal toxic shock syndromes and 9 streptococcal meningiocephalitis syndrome. The mortality was 81.25% and 11.11% respectively. Pathogenic bacteria isolated from diseased pigs and patients were found to have some common characteristics in morphology, staining and biological characters. Conclusion: The pathogen isolated from the blood of patients and pigs were identified as streptococci.     

硬膜外腔注射脉络宁对脊神经根炎大鼠血清中PRA和AT-Ⅱ的影响

目的:通过大鼠脊神经根炎模型,观察硬膜外腔注射脉络宁对血浆肾素活性(PRA)和血管紧张素(AT-Ⅱ)的影响.方法:用0.5%福尔马林浸泡的直径为0.5 cm的定量滤纸片作为致炎物,复制大鼠脊神经根炎模型.将动模随机分为5组,A组(正常组);B组(假手术组);C组(模型组)、M组(脉络宁组)和L组(利多卡因组).每只大鼠从硬膜外注药0.6 mL/kg,每4 d给药1次,一共给药4次.术后第18 d取血2 mL,然后用放射免疫分析法(RIA)进行PRA和AT-Ⅱ的测定.结果:C组PRA和AT-Ⅱ含量显著高于A组、B组、M组和L组(P＜0.01);硬膜外注入脉络宁和利多卡因可使血清PRA和AT-Ⅱ含量下降,两组比较无显著性差异(P＞0.05).结论:硬膜外腔注射脉络宁使药物能够高效快速地发挥作用,促进了炎性神经根的修复.     

髌骨鹰嘴化结合张力带钢丝治疗髌骨下极骨折

目的探讨应用髌骨鹰嘴化结合张力带钢丝治疗髌骨下极骨折的临床疗效。方法2001年6月～2004年11月应用髌骨鹰嘴化结合张力带钢丝治疗髌骨下极骨折34例,术后2～3周进行屈膝功能锻炼。结果28例随访9～30个月,平均21个月,按陆裕朴疗效评价方法:优21例,良5例,可2例,优良率92.9%。结论髌骨鹰嘴化结合张力带钢丝治疗髌骨下极可以恢复关节面的解剖关系,有利于膝关节早期运动,避免关节僵硬及创伤性关节炎的发生,是治疗髌骨下极骨折较好的方法。