成人骨髓间充质干细胞对脐血CD34+细胞的体外扩增作用

目的: 探讨骨髓间充质干细胞(MSCS)对脐血CD34 细胞体外扩增作用. 方法: 分离培养成人MSCS作为滋养层,联合SCF, IL-11和GM-CSF分别组成对脐血CD34 细胞的不同扩增体系,培养3 wk后观察脐血有核细胞、CFCs以及CD34 细胞扩增倍数的变化. 结果: MSCS联合细胞因子组较其他组培养体系对有核细胞总数、CFCs含量及CD34 细胞含量均具有明显的扩增作用,分别扩增了114.2±2.4, 40.5±8.6, 11.3±0.4 倍. 结论: 成人MSCS协同其他细胞因子可增强脐血CD34 细胞的体外扩增作用.     

基于UPLC-Q-Exactive Orbitrap-MS联合网络药理学探究双黄连解热抗炎的作用机制

目的:基于UPLC-Q-Exactive Orbitrap-MS方法,利用网络药理学和分子对接技术初步探究双黄连解热抗炎的物质基础及作用机制.方法:利用UPLC-Q-Exactive Orbitrap-MS技术鉴定双黄连口服液中的主要化学成分.基于网络药理学,将已定性化合物录入Swiss Target数据库筛选成分靶点...     

恶性孤立性肺结节患者电视胸腔镜手术治疗的预后情况及其影响因素分析

目的 分析恶性孤立性肺结节(SPN)患者电视胸腔镜手术治疗的预后情况及其影响因素。方法 150例行电视胸腔镜手术治疗的恶性SPN患者术后随访2年,统计生存与死亡情况。收集患者的临床资料,分析预后的影响因素。结果 150例恶性SPN患者随访期间死亡21例(14.00%)。死亡组的年龄、结节直径均显著大于生存组,家族史占比、吸烟史占比、高血压病史占比、 STK1水平及Ki-67阳性表达占比均显著高于生存组(P<0.05)。COX多因素生存分析显示,年龄大、有吸烟史、血清STK1水平高、 Ki-67阳性表达是恶性SPN患者电视胸腔镜手术治疗预后不良的危险因素(P<0.05)。结论 年龄大、有吸烟史、血清STK1水平高、 Ki阳性表达会增加恶性SPN患者电视胸腔镜手术治疗的不良预后发生风险,临床应重点关注此类患者并开展相应的防治措施,以有效改善患者的预后。     

我国首次发生小肠结肠炎耶氏菌病暴发流行

本文报道了兰州市1986年7月因食用被耶氏菌污染的病牛肉后,引起耶氏菌病的暴发流行。调查表明进食病牛肉205人,发病107人,发病率52.20％。平均潜伏期48小时,重病人主要表现腹痛、频繁腹泻,粘液脓血便,伴有发烧、恶心、呕吐。轻病人腹部不适,生身乏力及食欲差为主。发烧占总发病例数56.07％,;腹痛占58.88％;腹泻占41.12％,1/3病人有呕吐和恶心症状。 从病人粪便和食剩牛肉中分离到3株耶氏菌,检出率为42.85％。经鉴定均为O:3血清型和Wauters生物3型。分离菌株对庆大霉素、四环素、新霉素、卡那霉素和红霉素敏感,对磺胺类有抗药性。患者恢复半年后血清抗体GMT 1:52.78,同一人群健康者的抗体GMT 1:13.77。经口腔和腹内感染小鼠可引起腹泻。     

利用J+H噬菌体鉴定小肠结肠炎耶氏菌的评价

使用J+H噬菌体检查192株通过生化反应作出鉴定的小肠结肠炎耶氏菌和类小肠结肠炎耶氏菌,其裂解率为77.1％,比血清定型率(73.5％)为高,属于致病性血清型的裂解率为95.7％,其中O:3和O:9血清型38株的裂解率为100％;属于非致病性血清型的裂解率为59.6 ％。实验证明这株J+H噬菌体用于临床和流行病学调查致病性小肠结肠炎耶氏菌,非常简便和实用。     

小肠结肠炎耶尔森氏菌CNY选择性培养基的研究

<正> 近几年来,随着对Y.e菌研究的不断深入,一些选择性培养基相继问世,如CAL、BABY-4、AIY、YM和CIN等,但应用于从样品中分离Y.e菌,所得结果不能令人满意。为提高Y.e菌的检出率,我们研制了一种含有唑啉头孢菌素钠(Cefazoli-     

屈指肌腱损伤58例术后康复体会

目的：探讨有效治疗屈指肌腱损伤方法。方法：缝合后行主,被动功能锻炼及相应理疗。结果：５８例中４８ 例效果良好,５ 例满意。结论： 屈指肌腱损伤术后,应早期主动—被动活动加之物理疗法,是减轻肌腱粘连的重要措施。     

10种X-CT装置所致病人剂量的研究

Ｘ射线计算机断层摄影（ＸＣＴ）是当今医学影像诊断的重要手段。它在给人类带来巨大的利益同时,也给人们带来较大的剂量负担［１］。目前,我国已引进大量的ＸＣＴ装置,并且在全国范围内得到广泛的应用。为了评价ＸＣＴ检查给病人带来的辐射剂量,作者对国内应用...     

肾功能损伤分级系统在成人心脏术后的应用

Objective The aim of this study was to evaluate of adilty of two acute renal failure-specific scoring systenms (the classification by Bellome et al and the AKIN criteria) for predicting hospital mortality after cardiac surgery in adult patients. Methods Between October 1 st 2006 to Decemjber 31 st 2006, 509 adult patients who ungerwent coronary artery bypass grafting (CABG) and/ or valve operation were enrolled in this study. The medical data collection included gender, age, types of operation, perioperative he- modynamic parameters, urine output, biochemical parameters and outcome. Renal function was assessed daily according to the classi- ficatinn by Bellomo and the AKIN criteria, respectively. As references, Acure Physiology and Chronic Health Evaluation(APACHE) Ⅱ and Sepsis-related Organ Failure Assessment (SOFA) score were also calculated. Resuits Three hundred and forty-one patients were male (67.0%), and 168 were female (33.0%), mean age was (56.2±12.0) years old. Tnree hundred and nine patieats un- derwent CABG, 182 underwent valve operation and 18 underwent CABG plus valve operation, Mean duration of ventilation support was (20.4±17.7) houra, and the ICU stay was (1.4±1.0) days. Postoperative hospital stay was (13.8±9.1) days. According to the classification by Bellomo., the highest in-hospital mortality was 52.9% in ARFS group. Mahiplicatinn of in-hospital morality rate was abserved (X2 for trend, P<0.01) in 0.4% (non-ARF), 1.2% (stage 1), 12.0% (stal～ 2) and 32.4% (stage 3) of pa- tients based on the AKIN criteria. By applying the area under the receiver operating characteristic ourve, the classification by Bellomo and the AKIN criteria had good discriminative power. Furthering, multivariate logistic regression analysis verified that the Odds Ratio of the AKIN criteria was 5.478 (P =0.028, 95% Confidence Interval 1.027- 24.856), after adjusting for gender and age. Con- clusion Analytical data confinned good discriminative power of both the AKIN criteria and the classification by Bellomo for predicting hospital mortality of adult postoperative patient with ARF.