地塞米松复合聚己内酯胶原支架材料的构建及性能评价

BACKGROUND: Tissue engineering scaffold materials have been widely used in all kinds of tissue and nerve repair, but there are many limitations and the effect is not good. OBJECTIVE: To construct a kind of tissue engineering scaffold material for the regeneration and repair of spinal cord injury. METHODS: The dexamethasonemicrospheres were prepared by emulsification-solvent evaporation. The comprehensive scores of encapsulation efficiency, drug-loading rate and yield were taken as the indexes. The effect of dosage of dexamethasone and polylactic acid-glycolic acid copolymer and mass fraction of polyvinyl alcohol on formulation process of dexamethasone sustained-release microsphere was inspected by orthogonal experiment. The characterization of microspheres was observed by scanning electron microscope. The nanofiber scaffold of compound dexamethasone microspheres was prepared by taking collagen protein and polycaprolactone as raw materials using electrospinning technology. The mouse bone marrow mesenchymal stem cells were co-cultured with the scaffold for 3 days. Cell morphology was observed by scanning electron microscope. Composite material was implanted into the defect of spinal cord in rats. RESULTS AND CONCLUSION: The optimal preparation process of dexamethasone sustained-release microspheres: dosage of dexamethasone was 10 mg, dosage of poly lactic acid-glycolic acid copolymer was 80%, mass fraction of polyvinyl alcohol was 0.5%. Appearance of dexamethasone microspheres was smooth, with a round surface. The encapsulation efficiency, drug-loading rate and yield of microspheres were (2.26±0.03)%, (83.62±0.21)% and (90.87±2.45)% respectively. The growth of mouse bone marrow mesenchymal stem cells was good on the surface of compound dexamethasone microspheres. There was no immunological reaction between the implant material and host, and the material was degraded gradually with time. These results demonstrate that the compound dexamethasone microsphere scaffold has good biocompatibility, which is a favorable kind of biological scaffold material.        

三种石斛中微量元素测定及多糖提取工艺研究

目的测定三种石斛中微量元素的含量,并探讨鼓槌石斛多糖水提法的提取工艺,为其开发利用提供依据。方法采用全反射X射线荧光光谱仪对3种石斛中微量元素进行分析测定;探讨水提法提取多糖的工艺参数,用苯酚-硫酸法测定多糖含量。结果 3种石斛中富含对人体有益的Fe、Zn、Cu微量元素;最佳水提多糖的工艺条件为:料液比1:20、提取时间1 h、提取次数3次。结论 3种石斛的微量元素种类基本相同,且均富含Fe、Zn、Cu微量元素;优选得到的鼓槌石斛多糖水提工艺稳定可行。     

自体疣埋植治疗尖锐湿疣及其对机体T细胞亚群的影响

目的：研究自体疣组织埋植治疗尖锐湿疣（CA）疗效及其对机体细胞免疫功能的影响。方法：将98例尖锐湿疣患者随意分成两组，A组采用自体疣组织埋植＋CO2激光治疗，B组单用CO2激光治疗；两组治疗前后均检测外周血T淋巴细胞亚群。结果：A组复发率低，疗效优于B组，A组与B组比较，CD8^ 细胞数量明显下降（P＜0．05），CD4^ 细胞数量及CD4^ /CD8^ 比值明显提高（P＜0．05）。结论：自体疣组织埋植治疗尖锐湿疣疗效好，能降低复发率，其机理可能是自体疣组织埋植能改善CA患者的细胞免疫功能。     

巨大阔韧带肌瘤7例临床分析及文献复习

阔韧带肌瘤是子宫肌瘤的一种特殊类型,因缺乏特异性的临床表现,极易误诊为卵巢肿瘤.在临床上本病虽并不少见,但直径超过10 cm的巨大阔韧带肌瘤则较为罕见.体积过大的阔韧带肌瘤不但加大其手术的难度,而且易造成患者的心理负担.本文收集了2015年12月至2017年11月某院收治的7例巨大阔韧带肌瘤患者资料,结合文献复习,探讨本病的临床特征及诊治要点.     

清热解毒合剂治疗小儿手足口病合并脑炎40例效果观察

手足口病是由多种肠道病毒感染引起的以手、足、口腔等部位发生丘疱疹为主要特征的儿童传染病,主要病源是柯萨奇A16病毒、肠道病毒71型(EV71),其中EV71感染引起重症病例的比例较大,患儿可出现中枢神经损害,引发脑炎。中医药治疗本病有独特疗效,但大多数临床报道为手足口病轻     

精神科护患沟通的技巧

与患者进行良好的心理沟通是做好精神科护理工作的关键。如何掌握患者的心理,沟通就是主要的纽带。如何做好护患沟通,就是要很好地运用沟通技巧、沟通原则以及一些方式方法。     

胎儿频发室性期前收缩1例

孕妇２５岁 ,因妊娠３９周 ,孕１产０入院体检。胎心监测发现胎心音不规则。超声心动描记术检查示胎心率１５０次／ｍｉｎ ,胎心规则。为明确诊断 ,进行胎儿心电图检查 (灵敏度１／２,纸速２５ｍｍ／ｓ ,图１Ａ) ,示胎心率１４３次／ｍｉｎ ,其中ＲＳ型的为母亲ＱＲＳ波群 ;ｒｓ型的为胎儿ＱＲＳ波群 ,振幅较小 ,宽大畸形的Ｆ′为胎儿室性期前收缩。且可见每１、２个正常胎儿ＱＲＳ波群后即出现１个胎儿个室性期前收缩 ,形成二联律、三联律。产妇顺产一男婴 ,体重３２００ｇ,出生时Ａｐｇａｒ评分９分 (反射功能扣１分 ) ,５ｍｉｎ时评分１…     

丹脂消白饮联合窄谱UVB治疗白癜风疗效观察

将90例白癜风患者随机分成3组,治疗组(30例)给予中药丹脂消白饮联合窄谱UVB(NB-UVB)照射治疗;对照1组(30例)单纯NB-UVB照射;对照2组(30例),单用中药丹脂消白饮.3组均治疗3个月后进行临床疗效和不良反应评价.治疗组疗效明显优于对照1组和对照2组(均P<0.05).     

婴儿分支型室性心动过速诊治1例

女,2个半月,因咳嗽2d,发现心电图(ECG)异常2h入院。胸片：右下肺炎。肌酸激酶(CK)、CK-MB,肌钙蛋白、抗链“O”、电解质正常。心脏彩超正常。ECG：P波消失,QRS终末部分增宽、粗钝,时限0．11s,心室率167次／min,心电轴-56度,描记中恢复窦性心律,心房(室)率125次／min。动态心电图：     

脉压与心律失常关系的探讨

有资料表明心肌梗塞后心功能不全者,脉压(PP)越大,死亡率越高[1]。越来越多的资料表明,脉压增大是预测心血管事件独立危险因子[1,2]。但脉压增大是否会引起心律失常情况增加,国内外这方面的报道较少见。本文对180例无心室肥厚冠心病患者的临床资料进行分析、研究,以探讨脉压与心律失常的关系。