不同持续时间的周期性压力对构建组织工程软骨的影响

Objective To determine the best duration for exerting the cyclic pressure under which the tissue-engineered cartilage is constructed. Methods Free chondrocytes isolated from rabbit articular cartilage were seeded into polylactic acid-co-glycolic acid(PLGA) scaffolds after expansion in vitro, and ran-domized into 4 groups. In Groups 1 to 3, chondrocytes were cultured under daily cyclic pressure (0 ～ 200 kPa, 0.1Hz) for 4 hours, 8 hours, 12 hours respectively; Group 4 was a control in which no pressure was exerted. In each group, after 2 weeks of culture, the tissue engineered cartilages were observed in vitro and assessed by his-tological staining of liE. Next, the content of DNA and the secretion of type Ⅱ collagen and GAG in cartilages were detected quantitatively. Results Under the daily cyclic pressure (0 ～200 kPa, 0.1 Hz), the scaf-fold-chondrocytes complex in the group of 8 hours got the largest volume, smooth, lucidus, and elastic surface, the most queuing chondrocytes, and the highest content of type Ⅱ collagen and GAG (P < 0.01). Conclusions Since chondrecytes are baro-senstive, the metabolism of chondrocytes can be affected by the time of cyclic pressure. Under the effect of 0 ～200 kPa, 0.1Hz, the daily cyclic pressure of 8 hours may be optimal for chondrocytes to multiply and synthesize extracellular matrixes such as type Ⅱ collagen and GAG.     

股骨头缺血坏死的CT分区及临床意义

选择Ficat分期为Ⅱ、Ⅲ期股骨头缺血性坏死患者30例，依据X线片及CT片将坏死灶分／为前区、后区、全区、中区。30例患者均选择死骨刮除及带血运骨瓣植入术治疗．其中后区8例及中区3例选择死骨刮除植骨并股方肌肌肉血管蒂大粗隆骨瓣植入术治疗。结果平均获随访3．5a，依据王岩氏疗效标准：优25例，良5例。证实CT能明确诊断、显示坏死灶部位及量化分析病变范围．为临床分区提供可靠依据；指导准确选择手术入路及方法。分区手术是Ficat Ⅱ、Ⅲ期患者客观、理想的治疗方法。     

内镜下胸锁乳突肌切断松解术治疗先天性肌性斜颈4例报告

目的探讨内镜下胸锁乳突肌切断松解术治疗先天性肌性斜颈的方法和疗效。方法2005年1~8月,我院应用内镜下胸锁乳突肌切断松解术治疗先天性肌性斜颈4例,年龄5~11岁,平均8.5岁。患侧腋窝前缘置入10 mm trocar,在颈阔肌筋膜下、胸锁乳突肌胸骨头与锁骨头浅面钝性分离,注入CO2气体(压力6 mm Hg),建立颈前皮下间隙,置入30°内镜。分别在患侧颈后近锁骨上缘处及对侧胸壁近锁骨下缘处置入5 mm trocar至颈前皮下间隙,置入分离钳与电凝钩,距胸锁骨附着处1 cm电凝横断胸锁乳突肌胸骨头与锁骨头的肌纤维束,并松解胸锁乳突肌周围紧张的纤维组织。结果4例手术均获成功,手术时间分别为90、75、70、45 m in。术中出血均<1 m l。术后第1天开始功能锻炼,第2天出院。第1例颈部皮肤轻微电灼伤,2周后痊愈。1例术后出现面部皮下气肿,次日自行吸收。随访2、4、6、10个月,斜颈均矫正,切口小且隐蔽,瘢痕不明显,颈部皮肤弹性良好,对面部表情活动无影响。结论内镜下胸锁乳突肌切断松解术疗效确切,且具有微创的特点,值得临床推广。     

供者表达活化性杀伤细胞免疫球蛋白样受体对造血干细胞移植预后的影响

目的 观察供者表达活化性杀伤细胞免疫球蛋白样受体（aKIR）对受者造血干细胞移植（HSCT）预后的影响。方法 1996年至2001年共行亲缘性人类白细胞抗原（HLA）全相合骨髓移植59例，以序列特异性引物多聚酶链反应法（SSP-PCR）检测供者aKIR的表型。分析供者表达aKIR对受者移植后病毒、细菌和真菌感染及出血、复发、存活情况的影响。结果 供者表达aKIR与受者移植后出血、病毒及细菌感染发生的概率无明显相关性；当供者表达KIR3DS1表型时，发生真菌感染概率增高（X^4．804，P=0．028）。供者表达aKIR对受者HSCT后存活率和白血病复发率均无明显影响。结论 亲缘性HLA全相合HSCT中，供者表达aKIR并不能改善受者的移植效果。     

血管内栓塞外伤性颈动脉海绵窦瘘126例随访结果

目的探讨血管内栓塞治疗外伤性颈动脉海绵窦瘘（TCCF）的近期及远期效果。方法139例TCCF患者行血管内栓塞术后3个月开始通过再次入院、邮寄调查表或电话随访．其中再次入院完成及收回调查表共64例，电话随访62例，获随访126例（90．6％）。结果126例中26例术后行MRA检查，20例行颅脑CT检查，均未见异常；10例术后半年行DSA复查，未见复发。11例复发，经再次栓塞后治愈。结论血管内栓塞治疗TCCF，术后复发率不高，临床随访即可。     

恢复后期脑卒中综合康复治疗与单纯药物治疗的对比研究

目的 :研究综合康复治疗对恢复后期脑卒中患者运动功能及日常生活能力 (ADL)的影响。方法 :将 92例恢复后期脑卒中患者 ,随机分为康复组 5 0例 ,对照组 4 2例 ,两组一般治疗相同 ,康复组同时配合康复护理、运动疗法、作业疗法和电疗等综合康复治疗 8周。结果 :采用Fugl-Meyer运动功能评分 (FMA)和修订的Barthel指数 (MBI)评分 ,康复组各项指标评分明显优于对照组 (P <0 .0 5 )。结论 :综合康复治疗对于恢复后期脑卒中患者运动功能的恢复有明显效果 ,提高了ADL水平。     

下肢静脉交通支结扎加浅静脉硬化疗法治疗原发性下肢静脉瓣膜功能不全26例报告

目的：探讨下肢深浅静脉交通支结扎加浅静脉硬化剂法治疗原发性下肢静脉瓣膜功能不全的治疗效果。方法：采用腔镜下行下肢深浅静脉交通支结扎 浅静脉硬化剂法治疗260例原发性下肢静脉瓣膜功能不全。结果：26例病例均恢复良好，所有病例浅静脉曲张消失。结论：下肢深浅静脉交通支结扎加浅静脉硬化剂法治疗是下肢静脉曲张治疗方法的良好选择。     

不同持续时间的周期性压力对构建组织工程软骨的影响

Objective To determine the best duration for exerting the cyclic pressure under which the tissue-engineered cartilage is constructed. Methods Free chondrocytes isolated from rabbit articular cartilage were seeded into polylactic acid-co-glycolic acid(PLGA) scaffolds after expansion in vitro, and ran-domized into 4 groups. In Groups 1 to 3, chondrocytes were cultured under daily cyclic pressure (0 ～ 200 kPa, 0.1Hz) for 4 hours, 8 hours, 12 hours respectively; Group 4 was a control in which no pressure was exerted. In each group, after 2 weeks of culture, the tissue engineered cartilages were observed in vitro and assessed by his-tological staining of liE. Next, the content of DNA and the secretion of type Ⅱ collagen and GAG in cartilages were detected quantitatively. Results Under the daily cyclic pressure (0 ～200 kPa, 0.1 Hz), the scaf-fold-chondrocytes complex in the group of 8 hours got the largest volume, smooth, lucidus, and elastic surface, the most queuing chondrocytes, and the highest content of type Ⅱ collagen and GAG (P < 0.01). Conclusions Since chondrecytes are baro-senstive, the metabolism of chondrocytes can be affected by the time of cyclic pressure. Under the effect of 0 ～200 kPa, 0.1Hz, the daily cyclic pressure of 8 hours may be optimal for chondrocytes to multiply and synthesize extracellular matrixes such as type Ⅱ collagen and GAG.     

原发性食管小细胞癌治愈1例

原发性食管小细胞癌(DESCC)又叫雀麦细胞癌,是一种非常罕见的疾病。自1952年Mckeow首次报道第1例食管雀麦细胞癌以来,国内外陆续有人报道。我院采用综合化疗治愈1例食管小细胞癌.现报道如下。患者女.60岁.因吞咽不畅半个月于1990年7月24日入院。半月前无明显诱因出现吞咽不畅.尤其是进食半固体及固体饮食时梗阻感明显.伴有间断性胸骨后钝痛.逐渐发展到只能进食流质饮食。体检: