激光照射家兔眼前部组织的实验研究

用YAG、CO_2激光按不同功率照射家兔眼,观察对角膜、虹膜、晶状体的损伤和修复过程,探索两种激光对角膜的最小损伤和使其穿孔的有效功率。结果发现:YAG激光用230～250mW(1～2秒)时,可使角膜上皮呈灰白色混浊,1～2W(5～6秒)时角膜穿孔;CO_2激光用340～380mW(0.2～2秒)时,同样使角膜上皮灰白色混浊,1.3～1.4W(1秒)时角膜穿孔,在功率、照射时间、光斑相同的条件下,CO_2激光对角膜损伤重,YAG激光则对虹膜、晶状体损伤重。     

正常喉区的CT解剖研究

喉的解剖较复杂,从CT图象上分辨更为困难。木文通过比较尸体喉区的断层解剖标本,从4个不同的平面,描述了喉区各结构形态大小的变化,并讨论其分布特征,这将有助于临床CT的诊断。     

牙髓根管感染厌氧菌培养结果分析

从３４例牙髓根管感染中共分离出厌氧菌１１８株，经菌属分类依次为消化链球菌属，拟杆菌属，梭杆菌属，放线菌属，韦荣氏球菌属和珠网菌属，其中前３属为７７株，分离率为６５．３％，这一结果显示该３属细菌与极管感染有密切关系，是牙髓根管感染的优势菌。     

国产人α＿1型基因工程干扰素治疗慢性肝炎Ⅲ期临床试验

３９例慢性肝炎患者，其中乙型肝炎１２例，丙型肝炎２７例，接受人α＿１型基因工程干扰素治疗（ｒＨＩＦＮα＿１）。１０例丙型肝炎病人ｒＨＩＦＮα＿１每日肌注１×１０￣６Ｕ，连用９０天，１７例丙型肝炎病人和乙型肝炎ｒＨＩＦＮα＿１每日肌注３×１０￣６Ｕ，连用９０天。治疗后乙型肝炎组ＨＢｅＡｇ和ＨＢＶＤＮＡ的阴转率均为４１．６７％；丙型肝炎用１×１０￣６Ｕ／ｄ和３×１０￣６Ｕ／ｄ组ＨＣＶＲＮＡ阴转率分别为５０％和６４．７％，两个剂量组阴转率比较差异无显著性（Ｐ＞０．０５），治疗期间不良反应发生率较低，远期疗效尚待观察。     

胃癌患者胃液中5-羟色胺含量的检测及临床意义

本文应用高效液相色谱与荧光分光光度计联机法检测55例胃液内5-羟色胺的含量,共分为正常、溃疡病、萎缩性胃炎、胃癌等4个组。其结果正常组含量为48.70±49.3ng/ml,溃疡病组含量为76.26±127.06ng/ml,慢性萎缩性胃炎组含量为197.29±128.4ng/ml,胃癌组含量为191.80±124.2ng/ml。4组之间进行对比分析,正常组和溃疡病组之间差异不明显(P>0.05),而萎缩性胃炎和胃癌两组与前两组之间差异均显著(P<0.01),但萎缩性胃炎和胃癌之间未见差异(P>0.05)。这充分证明5-羟色胺(5-HT)在萎缩性胃炎和胃癌的胃液中含量是很高的。对于胃癌和慢性萎缩性胃炎的诊断及与其它慢性胃病的鉴别诊断中可能起到很重要的作用。     

交链孢酚诱发人胚食管上皮癌的研究

将体外培养的水囊引产的人胎儿食管用交链孢酚(Alternariol,AOH)处理24h,再培养两周,分别接种于裸小鼠皮下和腹腔内。皮下接种的3个食管组织块中之一形成鳞状细胞癌,其余2个和接种于腹腔的2个食管组织块上皮细胞呈明显增生或乳头状增生。空白对照组的食管组织块未见增生。溶剂对照组者仅呈轻度增生。结果提示互隔交链孢霉在人食管癌病因学中起着重要作用。     

银耳制剂对小鼠移植性肿瘤预防及其机理的实验研究

小鼠在移植肿瘤细胞前,注射银耳制剂,显示出银耳对荷腹水型或荷实体瘤小鼠肿瘤的生长有明显的抑制作用.正常小鼠给银耳制剂后,其腹腔巨噬细胞(M￠)数量与功能、形态有明显变化,此M￠可吞噬和杀伤肿瘤细胞.     

A study of tachykinin-immunoreactivity in the cat visual cortex

The localization of tachykinin-immunoreactivity in the cat visual cortex (area 17) was investigated using immunohistochemical methods. Strong laminar specificity was observed, with immunoreactivity highest in layer V, followed by layers I, VI, II and III, and the lowest density in layer IV. Most of the immunoreactive product was localized in neuronal processes. A few immunopositive cell bodies were also present. The immunopositive neurons were non-pyramidal, multipolar, or bipolar in shape, and mostly found in layer V. There were particularly dense immunopositive fibers and varicosities around somata in layer V. These may represent tachykinin-containing presynaptic terminals (boutons). The results provide anatomical evidence that tachykinin may primarily affect layer V neurons in the cat visual cortex.     

The relationship of serum IL-6 levels to acute graft-versus-host disease and hepatorenal disease after human bone marrow transplantation.

The potential involvement of cytokines in acute graft-versus-host disease led us to analyze interleukin-6 in serial serum sets from 22 allogeneic marrow recipients who developed either grade 3 or 4 GVHD (n = 10), grade 2 GVHD (n = 6), or grade 1 or no diagnosed GVHD (n = 6). A total of 279 serial serum samples taken three times weekly before day 35 were analyzed. Maximum IL-6 levels were greater than 40 U/ml (range, 40-1536 U/ml), 11-40 U/ml, and less than or equal to 10 U/ml for six, eleven, and five patients, respectively. Serum IL-6 peaks were temporally related to onset of GVHD, onset of a syndrome of hepatorenal dysfunction (HRD), or bilateral lung infiltration. Eight of ten patients who developed grade 3 or 4 GVHD overall had IL-6 maxima of greater than 10 U/ml an average of 1.5 +/- 1.8 days before the clinical onset. Fifteen of 17 patients with peak IL-6 levels greater than 10 U/ml developed symptoms of hepatic and renal dysfunction within three days of the peak, while none of five patients with less than or equal to 10 U/ml of Il-6 developed HRD. Regression analysis demonstrated a linkage between the log magnitudes of the serum IL-6 peaks and onset of either GVHD or HRD within three days (P = 0.001). Furthermore, IL-6 peaks tended to precede GVHD onset for the 10 patients whose GVHD onset and IL-6 peak were within three days of each other (P = 0.02). These results, confirmed by both specific bioassay and by IL-6 ELISA, support the idea that acute GVHD in humans involves a cytokine cascade that includes production of IL-6 in addition to the previously reported involvement of tumor necrosis factor alpha and interferon-gamma.