氯丙咪嗪、帕罗西汀、万拉法新治疗老年抑郁症的对照研究

目的:比较氯丙咪嗪、帕罗西汀、万拉法新治疗老年抑郁症的疗效和不良反应。方法:对符合CCMD-Ⅲ抑郁发作诊断标准的患者随机分为3组,每组30例,分别给予氯丙咪嗪、帕罗西汀和万拉法新治疗,疗程6周。用Hamilton抑郁量表(HAMD),临床大体印象量表(CGI)、不良反应量表(TESS)进行评分。结果:氯丙咪嗪显效率70%,帕罗西汀显效率65%,万拉法新显效率75%,差异无显著性(x2=0.42,P>0.05)。万拉法新显效快,2周内的HAMD总分低于氯丙咪嗪和帕罗西汀组。结论:万拉法新是一种疗效较好而不良反应较少的治疗老年抑郁症的药物。     

人组织型纤溶酶原激活剂(t-PA)与绿色荧光蛋白(GFP)在转基因烟草中的融合表达

构建人组织型纤溶酶原激活剂(t-PA)与绿色荧光蛋白(GFP)融合表达的植物表达载体pCA1302NSF-tPA。将重组载体转入根瘤农杆菌GV3101中，通过叶圆盘法转化烟草。转化植株经PCR鉴定整合有t-PA基因，通过荧光显微镜可检测到绿色荧光蛋白(GFP)的表达。     

Identification and Determination of Microcystins in Source Water an Waterbloom Sample From Meiliang Bay, Taihu Lake, China

OBJECTIVE: To identify and determine the congener and level of microcystins in the source water of Taihu Lake. METHODS: Improved method of SPE combined with HPLC was employed to detect the concentration and varieties of microcystins in source water and bloom samples collected from Meiliang Bay, Taihu Lake. RESULTS: The contents of two predominant microcystin components, MC-RR, and MC-LR, were relatively high in samples during warm months and correlated with the phase of algae growth. The maximum concentrations of MC-RR and MC-LR in water sample reached 3.09 +/- 0.53 microg/L and 2.39 +/- 0.41 microg/L during the period of water bloom in September 2004, respectively. Even without waterbloom, the concentration of MC-LR in source water sample was still higher than the guideline value. CONCLUSION: The status of microcystin pollution in this region is serious and measures to monitor and control the growth of cyanobacteria are urgently needed.     

Effect of HIF-1α on VEGF-C Induced Lymphangiogenesis and Lymph Nodes Metastases of Pancreatic Cancer

The effect of hypoxia inducible factor-1α(HIF-1α) on vascular endothelial growth factor C (VEGF-C) and the correlation between HIF-1αand lymphangiogenesis and lymph nodes metasta-ses (LNM) in pancreatic cancer were investigated. Immunohistochemical SP method was used to detect the protein expression of HIF-1αand VEGF-C, and Lymphatic vessel density (LVD) was determined by stain of VEGFR-3, collagen type IV in 75 pancreatic head cancers from regional pancre-atectomy (RP) during Dec. 2001 to Dec. 2003. The relationship between HIF-1αand VEGF-C, lymphangiogenesis, LNM was analyzed statistically. The results showed that the positive expression rate of HIF-1αand VEGF-C in pancreatic cancer tissues was 48.00 % (36/75) and 65.33 % (49/75) respectively. In positive group of HIF-1α, the positive rate of VEGF-C and LVD, and LVD rate was 80.56 % (29/36), 13.22±3.76 and 88.89 % (32/36) respectively, and in negative group of HEF-1α, positive rate of VEGF-C and LVD was 51.28 % (20/39), 5.98±2.17 and 66.67 % (26/39) respectively (P<0.01 or P<0.05). It was suggested that HIF-1αcould promote the expression of VEGF-C, lymphangiogenesis and LNM in pancreatic cancer.     

成人肺结核不典型影像学表现分析

目的研究成人肺结核不典型的影像学表现,探讨诊断中存在的问题。方法回顾性研究分析了32例成人肺结核的X线、CT表现,其中6例做CT增强扫描,8例做纤维支气管镜检查,全部经临床或病理确诊。结果肿块型8例,表现为叶、段实变型6例,粟粒结节型5例,支气管内膜结核6例,空洞型4例,下叶结核3例。共误诊14例,其中误诊为肺癌7例,误诊为肺炎6例,1例误诊为转移癌。结论肺结核的发病出现上升趋势,发病率升高,老年结核增多,X线、CT表现各有特点,检查要全面,切勿单纯依赖一种检查,要密切结合临床,才能减少误诊。     

氨基胍对胶原酶诱导脑出血大鼠模型的影响

目的研究氨基胍对脑出血的影响。方法以Ⅶ胶原酶诱导脑出血大鼠模型,观察动物行为学和脑内血肿的变化,用Western blot检测诱导型一氧化氮合酶蛋白表达的变化。结果氨基胍能明显加快脑出血大鼠行为缺陷的恢复,但对于血肿吸收没有明显影响;Western blot显示:氨基胍能明显抑制脑出血大鼠患侧皮质和基底节诱导型一氧化氮合酶蛋白表达。结论通过广泛抑制脑内诱导型一氧化氮合酶的表达,氨基胍能促进脑出血大鼠的运动功能恢复。     

“党义”与“风化”之间——从审查制度看民国时期政治对唱片的影响

中国的唱片审查制度是由南京国民政府创立的,1931年6月颁布的《上海市教育局审查戏曲·唱片规则》是中国历史上第一部针对唱片内容制订的法规。相关法规之所以出台,一方面是由于唱片业自身的发展所致,更重要的则是国民党将其视为改造社会的一环而刻意作为的结果。故终民国之世,不得违背“党义”,不能有伤“风化”,始终是唱片审查制度的核心与底线。本文以民国时期的唱片业中心上海为例,对上海特别市、日伪、抗战胜利后三个历史时期的唱片审查制度逐一进行论述、对比,探讨制度背后的政治与社会背景,并对其与唱片业之间的互动关系进行了分析。     

对医学类高职高专院校计算机基础教育的思考

探讨了目前医学类高职高专院校计算机教育问题,通过分析医学高职高专院校计算机教学的目的和现状,对解决目前存在的一些问题做了初步思考.     

多层螺旋CT在骨外伤中的应用价值

目的:探讨16层螺旋CT图像后处理在骨外伤的应用价值.方法:68例骨外伤患者进行多层螺旋CT扫描,扫描层厚为5mm;螺距为1.375:1;将原始数据重建为层厚1.25mm;间隔为0.625mm(重叠率50%).将重建图像传输至GE公司AW4.2图像后处理工作站,应用表面遮盖显示(SSD)、多平面重建(MPR),对图像进行双盲法分析.结果:68例骨外伤中,SSD与MPR均提供了准确的影像诊断.结论:MSCT检查,特别是三维和MPR重建对骨关节损伤的正确诊断和分型,对于临床治疗方案的制定均有重要价值.     

微量元素硒的研究进展

硒是动物机体必需微量元素之一,在机体内发挥着十分重要的生物学功能。研究发现,有机硒相对于无机硒毒性小、生物利用率高、生物活性强。就硒、有机硒的研究近况展开综述。