细胞角蛋白单克隆抗体免疫组织化学染色方法检测大肠癌淋巴结微转移的研究

目的为研究角蛋白克隆抗体免疫组织化学法在检测淋巴结微转移灶上的意义。方法采用三种角蛋白单克隆抗体AE1,AE3、AE1/AE3,对我院47例大肠癌术后患者331个淋巴结用免疫组织化学法重新检查。结果36个淋巴结存在微转移灶(11.9%),Duke'sC期患者微转移灶检出率高于Duke'B期患者(P<0.05)。随访显示有微转移灶Duke'B期病人肿瘤复发、远处扩散转移均较无微转移病人高(P<0.05)。结论即使是组织学检查结果为阴性的淋巴结,也很有存在微转移的可能性。免疫组织化学法栓测单克隆抗角蛋白抗体,可详细阐明临床分期,并可提供大肠癌治疗的指征。     

中西医结合治疗慢性乙型肝炎病毒携带者65例临床观察

目的:观察温肾解毒协定方联合干扰素治疗肾虚毒伏型HBeAg阳性慢性乙型肝炎病毒携带者的临床疗效。方法:将125例慢性乙型肝炎病毒携带者分为治疗组与对照组,治疗组65例采用温肾解毒协定方合干扰素治疗,对照组60例单用干扰素治疗,疗程均为48周。观察两组中医证候疗效及治疗后外周血白细胞计数、HBV DNA、HBeAg/HBeAb、肝功能的变化情况。结果:中医证候疗效总有效率治疗组为87.7%,对照组为63.3%,组间比较,差异有统计学意义(P0.05);治疗后治疗组HBV DNA阴转率、HBe Ab/HBeAg血清转换率及肝功能ALT、TBiL检测结果与对照组比较,差异均有统计学意义(P0.05)。结论:温肾解毒协定方联合干扰素治疗肾虚毒伏型HBeAg阳性慢性乙型肝炎病毒携带者的疗效明显优于单用干扰素治疗。     

一期前后联合入路治疗重度脊髓型颈椎病的疗效分析

目的探讨前后路联合手术治疗脊髓型颈椎病(CSM)的临床效果。方法回顾性分析2006年9月—2009年2月本院采用一期前后路联合手术治疗42例重度CSM患者,男32例,女10例;年龄41～76岁,平均58.5岁。前路减压涉及1个椎间隙者8例,2个椎间隙者28例,涉及3个椎间隙及以上者6例。本组患者均采用一期颈后路单开门椎管扩大成形和前路间盘摘除减压植骨内固定术。依据日本骨科学会脊髓功能17分法(JOA 17)标准,观察并记录患者的神经功能恢复情况,比较术前、术后3个月及末次随访时的JOA 17评分,评价临床治疗效果。结果手术时间为2.5～4.0 h,平均3 h。术中出血200～800 ml,平均400 ml,无严重手术并发症。所有患者定期随访,随访6～21个月(平均18.2个月),前路植骨及后路门轴全部骨性愈合,术前患者JOA 17评分为(6.42±1.22)分,术后3个月为(12.24±0.55)分,末次随访为(13.17±0.68)分,术前、术后3个月及末次随访时JOA 17评分间差异有统计学意义(F=748.37,P<0.05);前后路联合手术改善率为(72.31±5.33)%。结论对前后受压的重度CSM...     

糖尿病患者血小板功能检测与下肢血管病变的相关研究

本研究采用全血流式细胞仪和超声多谱勒高频探头，检测我院100例2型糖尿病患者血液中血小板功能和双下肢动脉血流参数，并与正常组对照比较，探讨2型糖尿病患者血小板功能与大血管病变的关系。     

局部温热并埋封阿霉素微脂粒治疗乳腺癌的研究

目的　观察局部温热并埋封热敏性微脂粒治疗乳腺癌的作用。方法　采用局部温热并埋封热敏性阿霉素微脂粒 (HT Ts LiP ADM )治疗乳腺癌 40例 ,以环磷酰胺、阿霉素、5 氟尿嘧啶 (5 FU)联合方案治疗乳腺癌 2 0例作对照 (CAF组 )。结果　 1疗程后 ,HT Ts LiP ADM组完全缓解 +部分缓解 (CR +PR) 3 4例 ,好转 (MR)4例 ,稳定 (SD) 2例 ,总有效率 85 % ,未见病变进展。CAF组CR +PR 8例、MR 6例 ,SD 4例 ,总有效率 40 % ,2例病变进展。两组比较HT Ts LiP ADM组疗效明显提高 (P <0 0 1) ,其毒性反应、恶心呕吐、心脏毒性及骨髓抑制明显减少。结论　局部加热并埋封阿霉素微脂粒治疗乳腺癌有协同作用     

艾滋病并发皮肤隐球菌病及隐球菌性脑炎一例误诊分析

1 病例资料 男,38岁,未婚.因头痛2个月,加重伴视力下降1周入院.2个月前无明显诱囚出现左颞部间歇性隐痛,时轻时重,发作逐渐频繁.1周前病情明显加重,出现持续性头胀痛,以前额及双眼球后为甚,伴视力下降.患者到某眼科医院就诊,眼底检查示:双视盘水肿,边界不清,表面及边缘有出血灶,视网膜周闱水肿,呈放射条纹状,静脉迂曲.     

艾滋病并发隐球菌病1例

隐球菌病是由新型隐球菌感染引起的深部真菌病.新型隐球菌为条件致病菌,通常不感染正常人,免疫功能低下者易感染榷患.我院于2008年9月收治1例艾滋病并发隐球菌病患者,经中西医结合治疗好转,现报道如下,并对相关文献进行复习和总结.     

早期运动训练联合神经干细胞移植对脊髓损伤模型大鼠后肢运动功能的影响

BACKGROUND: Studies have shown that neural stem cell transplantation combined with exercise training can promote the recovery of hindlimb motor function from spinal cord injury in rats, but its mechanism of action has not been fully elucidated. OBJECTIVE: To investigate the effects of early exercise training combined with neural stem cell transplantation on the recovery of hindlimb motor function in rats with spinal cord injury. METHODS: Sixty Sprague-Dawley rats with spinal cord injury were randomly divided into three groups: control group (n=20, given conventional treatment after injury), cell transplantation group (n=20, given neural stem cell transplantation after injury), experimental group, (n=20, given neural stem cell transplantation combined with early exercise training after injury). Recovery of the hindlimb motor function was assessed by Basso, Beattie and Bresnahan scale and inclined plane test before and at 1, 7, 14, 21 days after injury. Western blot assay was used to detect caspase-3 and myeloperoxidase expression. Hematoxylin-eosin staining was done at 21 days after injury to observe the structure changes of the injured spinal cord. RESULTS AND CONCLUSION: (1) Scores of Basso, Beattie and Bresnahan scale and inclined plane test were significantly better in the experimental group than the cell transplantation group followed by the control group (P < 0.05). (2) In the control group, the expression of caspase-3 and myeloperoxidase was significantly increased at 14 days after injury. In the cell transplantation, the expression of caspase-3 and myeloperoxidase was significantly higher than the experimental group (P < 0.05). (3) Pathological inflammation was reduced most in the experimental group followed by the cell transplantation group. In the experimental group, the structure of injured spinal cord was improved and became relatively clear and intact. These findings indicate that neural stem cell transplantation combined with early exercise training can effectively promote the recovery of hindlimb motor function from spinal cord injury in rats, by reducing the expression of caspase-3 and myeloperoxidase and alleviating secondary lesion of the spinal cord.      

安脑平冲汤联合泮托拉唑治疗脑出血急性期昏迷患者应激性溃疡的临床观察

目的研究脑出血急性期昏迷患者应用安脑平冲汤和泮托拉唑干预的作用。方法选择Glasgow昏迷量表评分（GCS）≤8分脑出血急性期合并应激性渍疡患者302例，随机分为试验组（115例）与对照组（187例），所有患者基础治疗措施相同，对照组和试验组均静脉使用泮托拉唑，试验组并鼻饲安脑平冲汤。结果试验组存活46例，病死率60．00％，存活患者恢复清醒者34例，平均恢复清醒时间126．41h&#177;48．16h，优于对照组（P〈0．05）。试验组治疗前后中医证候积分、胃液0B阳性率、胃液pH值比较均有统计学意义（P〈0．05或P〈0．01）；两组治疗后中医证候积分比较有统计学意义（P〈0．05），胃液OB阳性率及pH值改变呈时效相关性。两组治疗后7d、14d胃液pH值比较无统计学意义（P〉0．05）。结论安脑平冲汤联合泮托拉唑能有效治疗脑出血急性期昏迷患者合并应激性溃疡。