乳腺浸润性微乳头状癌组织中 MMP-9、uPAR 和 VEGF-C 蛋白的表达和意义简

目的探讨乳腺浸润性微乳头状癌中MMP-9、uPAR和VEGF—C蛋白的表达与临床病理特征的关系。方法应用免疫组化SP法检测57例乳腺浸润性微乳头状癌中MMP-9、uPAR和VEGF—C蛋白的表达情况。结果MMP-9、uPAR和VEGF—C蛋白的表达率分别是84．2％（48／57）、77．2％（44／57）和80．7％（46／57）,相互之间表达差异无显著性（P〉0．05）。MMP-9、uPAR和VEGF—C蛋白的表达与年龄、肿瘤所占比例无相关性（P〉0．05）,其在Ⅱ-Ⅲ级IMPC中表达显著高于Ⅰ级（P〈0．05）,在超过3个淋巴结转移的IMPC中的表达显著高于不超过3个淋巴结转移的IMPC（P〈0．05）。结论MMP．9、uPAR和VEGF—C蛋白在IMPC中高表达,组织学级别越高,淋巴结转移数越多,表达越高。     

建立肺血减少型先心病幼猪动物模型评估肺血管发育

目的 采用球囊扩张人工房间隔造口加肺动脉环缩术建立肺血减少型先心病幼猪动物模型,探讨肺血减少对其未成熟肺血管发育病理形态学变化的影响.方法 选用1～2月龄幼猪共20头,随机分为3组:正常对照组(C组,6头),右胸小切口造成一过性肺血减少;轻中度肺动脉狭窄组(T1组,7头),经右心房表面送入球囊扩张器行人工房间隔造口加肺动脉Banding环缩术,术中收缩期肺动脉环缩处压差(Trans-PABP)20～30 mm Hg(1 mm Hg=0.133 kPa);重度肺动脉狭窄组(T2组,7头),术中Trans-PABP≥30～50 mm Hg.术后超声评估、1个月时行64排CT扫描评估、2个月时行二次剖胸手术验证评估,测量各组血管近端直径及Trans-PABP,处死动物切取心、肺组织测量房间隔缺损、肺动脉和Banding环直径.手术结束、术后2个月取右肺中叶外侧段1.0 cm ×0.8 cm×0.8 cm肺组织,Weihgt弹性纤维+Van Gieson染色,光镜下观察其病理形态学变化.结果 T1和T2组存活动物房间隔造口加肺动脉环缩术均获得成功.C组动物因麻醉意外术后10 h死于呼吸衰竭1头;T1组术后21天死于肠梗阻、肠坏死1头;T2组死亡2头,分别因急性和慢性右心功能衰竭,于术后24 h和39天死亡.64排CT扫描示,肺动脉环缩处直径(BD)显著低于其主动脉直径(AOD)(P＜0.01),近端狭窄后扩张、远端肺血管纹理稀少.肺组织病理学示术后2个月肺细小动脉内径T1、T2组明显高于C组(P＜0.05、P＜0.01),单位面积小动脉数量(NAPSC)实验组均显著低于对照组(P＜0.01).结论 构建的幼猪动物模型更接近于临床病理生理状态,64排螺旋CT联合肺组织病理学观察用于肺血管发育不良的评估是一种可靠方法.肺血减少时幼猪肺小动脉中层发育不良伴数量减少,随环缩加重肺动脉发育不良和肺血流的减少的程度逐渐加重.

Abstract：

Objective Artificial atrial septum defect combining pulmonary artery banding to create a model of congenital heart defect with decreased pulmonary blood flow to explore the morphological changes of immature pulmonary vascular. Methods Choose twenty piglets with about one to two-month-old, which are exclusively for experiment used. The piglets were randomly divided into three groups: normal control group (group C, n = 6), Small incisions on the right chest, produced a transient reduction in pulmonary blood; low-medium pulmonary artery stenosis groups ( group T1, n = 7 ) : Did artificial room septostomy creation by self-dilators which were delivered into the surface of the right atrium and controlled Systolic trans pulmonary artery banding pressure (Trans-PABP) at pressure of 20 - 30 mmHg; severe pulmonary artery stenosis groups ( group T2, n = 7): T2 were the same surgical procedures with group T1 ,and controlled Trans-PABP ≥ 30 -50 mmHg. Monitored ultrasound after operation , carried out 64-slice computed tomography scanning after one month, to measure the proximal vessel diameter and TransPABP , after two month surgical exploration on the left chest. When the animals were sacrificed, the heart and lung tissue was cut to measure atrial septal defect, pulmonary artery and the banding diameter. By weihgt elastic fiber and van Cieson staining to observe the morphological pathological changes, three groups took lung tissue with right middle lobe lateral segment about 1.0 cm × 0.8 cm × 0.8cm at the end of surgery and 2-months after operation respectively. Results The models were all successfully in the survival animal of the two test groups. One pig died from tracheal intubation accident in the C group, there was one case died due to bowel obstruction in the T1 group, And there were two cases died result from acute right heart failure and chronic heart failure respectively in T2 group. 64-slice CT angiography showed that BD was significantly lower than the AOD in the two test groups, the proximal pulmonary vascular expansion result from stenosis, distal pulmonary vascular scarce. Histopathology showed that the pulmonary artery inside diameter of T1 and T2 was significantly higher than group C(P ＜0. 05,P ＜ 0.01), and the NAPSC of two experimental groups were significantly lower than group C 2-month after operation( P ＜0.01).Conclusion This type of Piglet model is closer to clinical pathological and physiological ,64-slice spiral CT combined with lung histopathology observed for the evaluation of pulmonary vascular hypoplasia is a reliable method. Tunica media of pulmonary arterioles hypoplasia with the number reducing, with pulmonary artery banding increased,the degree of pulmonary arterioles hypoplasia gradually increased.     

claudin-1在胆囊癌中的表达及意义

目的:检测claudin-1蛋白在原发性胆囊癌中的表达情况,并分析其与临床病理参数的可能关系。方法:收集胆囊癌标本47例、胆囊腺瘤18例、慢性胆囊炎15例。应用免疫组织化学方法检测claudin-1蛋白的表达情况,同时收集胆囊癌患者的临床病理资料,并分析结果。结果:在胆囊癌、胆囊腺瘤及慢性胆囊炎组织中claudin-1的阳性表达率分别为89.4%、27.8%和26.7%,差异有统计学意义(P0.01),胆囊腺瘤组与胆囊炎组差异无统计学意义(P0.05)。胆囊癌患者的年龄、性别、肿瘤的组织学类型间claudin-1表达差异均无明显不同(P0.05)。病理Ⅱ～Ⅲ级标本中claudin-1表达高于Ⅰ级(P0.01),Nevin分期Ⅳ～Ⅴ期中表达高于Ⅰ～Ⅲ期(P0.05),有转移者高于无转移者(P0.05)。结论:claudin-1在胆囊癌的发生、侵袭及转移机制中发挥重要作用。     

软组织骨化性纤维黏液样肿瘤2例报道及文献复习

目的 探讨软组织骨化性纤维黏液样肿瘤(ossifying fibromyxoid tumor of soft tissue,OFT)的临床病理、免疫表型特征及其诊断和鉴别诊断.方法 对2例OFT临床病理资料进行分析、光镜观察及免疫组化标记,并复习相关文献.结果 2例患者临床表现均为皮下缓慢生长的肿块.手术完整切除肿块,大体标本表面均有一层不完整的骨壳.术后病理组织学检查见肿瘤边界清楚,有纤维性假包膜,包膜内有一层薄的不连续的由成熟化生的板层骨构成的骨小梁,肿瘤略呈分叶状,内见有圆形、卵圆形、短梭形瘤细胞呈巢状、索状、网格状、镶嵌状排列,核染色质细致,未见明显核分裂象,有明显纤维黏液样背景的间质.免疫表型:vimentin、S-100蛋白均(+),CK(-).结论 OFT是一种分类尚未确定的罕见肿瘤,属中间型肿瘤.其特征性的骨壳结构、独特的细胞形态及排列方式、免疫表型具有诊断意义.     

乳腺癌pS2、neu癌基因表达与淋巴结转移的关系

目的:探讨女性乳腺癌pS2、neu的表达及其意义. 方法:应用免疫组化SP法对65例乳腺癌组织蜡块进行回顾性检测. 结果:在65例乳腺癌中,pS2阳性表达率为50.8%;neu阳性率为46.2%,且淋巴结转移组和未转移组间差异有显著性(P＜0.01). 结论:neu基因蛋白的过表达与淋巴结转移关系密切,检测neu表达情况可以作为判断乳腺癌患者预后的重要参考指标之一.     

舒林酸抑制胃癌BGC-823细胞生长的实验观察

目的:观察舒林酸对胃癌BGC-823细胞生长的抑制作用,探讨其作用机制.方法:将舒林酸设置不同的作用浓度和作用时间于人胃癌BGC-823细胞共培养.采用MTT比色法检测胃癌细胞抑制率,流式细胞术检测胃癌细胞周期分布,TUNEL法检测细胞凋亡率,免疫组化检测细胞增殖(ki-67)、凋亡抑制基因(survivin)及还氧合酶(COX-2)蛋白的表达.结果:舒林酸使胃癌BGC-823细胞的生长受抑制,出现G0/G1期比例增高,S期比例降低,同时使细胞凋亡率显著上升;而ki-67、survivin及COX-2蛋白表达阳性率显著降低,上述作用均呈时间和剂量依赖性(P＜0.01).结论:舒林酸可抑制胃癌BGC-823细胞生长,其机制涉及影响细胞周期分布、诱导细胞凋亡及抑制COX-2、ki-67及survivin蛋白的表达.     

以VEGF、MMP-2检测验证幽门螺杆菌L型感染与胃癌的关系

目的研究幽门螺杆菌L型(Helicobacter pyloriL-form,Hp-L型)感染对胃癌的发生、发展和侵袭、转移等生物学行为的影响,并探讨其机制。方法应用革兰染色和免疫组化SP法检测130例胃癌和50例对照组的Hp-L型感染,应用免疫组化SP法检测上述组织的血管内皮生长因子(vascular endothelial growth factor,VEGF)、基质金属蛋白酶-2(matrixmet-alloproteinase-2,MMP-2)蛋白的表达。结果130例胃癌中免疫组化及革兰染色同时Hp-L型阳性的病例有88例。胃癌组与对照组的Hp-L型阳性率相比具有统计学意义(67.69%vs24%,P<0.05)。胃癌组中Hp-L型阳性组的MMP-2、VEGF表达阳性率高于其Hp-L型阴性组(P<0.05);胃癌组中Hp-L型阳性MMP-2和VEGF的表达阳性呈正相关(r=0.45,r=0.30,P<0.001)。结论Hp-L型感染是影响胃癌侵袭和转移的重要因素之一。其机制与胃癌细胞的VEGF、MMP-2的表达上调有关。     

绝经后子宫出血与细菌L型感染的关系

绝经后子宫出血是老年妇女常见病症,有关绝经后子宫出血与细菌Ｌ型感染的关系报道甚少。本文应用组织病理学、细菌学和免疫组织化学染色等方法,对８３例绝经后子宫出血的内膜组织进行回顾性研究,就细菌Ｌ型感染与出血的关系等问题进行探讨。１资料与方法１．１资料收集近３年蚌埠医学院病理教研室外检档案中绝经后子宫出血患者的刮宫内膜组织共８３例,年龄最小４９岁,最大７１岁,平均６１岁。１．２方法所有标本均用１０％甲醛固定,石蜡包埋,连续切片,厚４ｕｍ,分别进行ＨＥ染色．革兰染色,并对其中部分病例进行免疫组织化学染色…     

鼻咽癌,喉癌中细菌L型感染及P^53,CerbB—2基因突变的对比研究

用革兰染色和免疫组化染色技术，对１２９例鼻咽癌，８５例喉癌，２０例鼻咽慢性炎，２０例声带息肉进行细菌Ｌ型检测，同时对Ｌ型阳笥和阴性组织的Ｐ＾５３，ＣｅｒｂＢ－２表达进行对比分析，结果显示，鼻咽癌，喉癌的革兰染色Ｌ型检出经分别为７８．３％和７６．５％，肿瘤组的Ｐ＾５３，ＣｅｒｂＢ－２表达率明显高于炎症组。鼻咽癌，喉癌中Ｌ型阳性组的Ｐ＾５３，ＣｅｒｂＢ－２的表达率也高于其Ｌ型阴性组。表明Ｌ型感染与鼻咽