姜黄素对白血病K562细胞增殖抑制作用的研究

目的:探讨姜黄素(curcumin)对白血病K562细胞增殖抑制的作用机制。方法:采用MTT法测定curcumin对K562细胞增殖的抑制作用;分别应用AO/EB和MDC荧光染色观察给药后凋亡和自噬的发生;检测LDH漏出率以观察用自噬特异性抑制剂3-MA后curcumin抑瘤作用的变化。结果:Curcumin可显著抑制K562细胞生长,诱导其发生自噬和凋亡;给药后阻断自噬其细胞毒性作用减弱。结论:curcumin能诱导白血病K562细胞的自噬和凋亡从而提高了抗癌的敏感性。     

颈椎病源性肌萎缩的诊断及治疗

肌萎缩型颈椎病 (ＣＳＡ)的临床特点为较重的上肢肌萎缩而无感觉障碍。我院自 1998年 7月至 2 0 0 0年 4月 ,共诊治 5例 ,取得一定疗效 ,报告如下。1　临床资料本组男 4例 ,女 1例 ,年龄 4 2～ 6 3岁 ,病程 1个月～ 1 5年。均表现为一侧肩胛带肌肉萎缩无力 ,肩外展明显受限而无感觉障碍。肌电图检查提示受损肌肉 (多为Ｃ5、6支配区 )运动电位多相性与巨大电位。Ｘ线片见增生骨棘形成等改变 ,Ｔ2 加权ＭＲＩ可见颈神经前根受压或脊髓内高信号区。 3例行保守治疗 ,包括应用颈部支具、牵引再辅以理疗、激素、Ｂ族维生素等 ,随访 10个月肌力由 2…     

血液透析患者合并感染性心内膜炎一例报道及文献复习

Brescia等[1]首次于1966年报道了血液透析患者合并感染性心内膜炎(IE).血液透析患者IE的发生率高于普通人群.现分析了我院2012年收治的1例血液透析合并IE患者的临床特点,以期提高临床医生对该疾病的认识. 患者 男,49岁.因"糖尿病20年,泡沫尿伴浮肿2年,喘憋1周"于2012年3月首次入院.查体:血压180/120 mm Hg(1 mm Hg=0.133 kPa),贫血貌,右侧肩胛下角线第7肋间以下叩诊浊音,心界向左扩大,未闻及心脏杂音,双下肢重度水肿.     

维持性血液透析患者血清瘦素/脂联素比值与心脏脚踝血管指数的相关性

动脉硬化性心血管疾病是终末期肾脏病(ESRD)患者的主要并发症及死亡原因.瘦素/脂联素比值可作为评估动脉粥样硬化进展的血清学标志[1-2].该指标在维持性血液透析(MHD)患者中的研究目前少有报道.本研究以心脏脚踝血管指数(cardio-ankle vascular index,CAVI)作为评估动脉硬化指标,研究瘦素/脂联素比值与CAVI的相关性.     

先天性马蹄内翻足动物模型胚胎后肢(芽)评分系统的建立

Objective Quantification of the severity of congenital clubfoot is essential for its re-search and treatment. Up to date, no scoring system is available for hind limb bud development. In this study,a scoring system was established based on a murine model Methods Sixty female Wistar rats weighing 220～250 g were randomized into the six control groups and six experimental groups. Vaginal sperm plug detection day was considered to be day 0 of gestation. Hind limb buds from fetuses of E13, 14, 15,16,17 and 18 days were removed and processed. Results Morphogenetic differentiation changes were detected in the experimental groups. According the new scoring system, clubfoot-like deformity was present in 80.9% of the experimental fetuses. We were able to quantify the extent morphogenetic diffcrentiation of the hind limb accordingly. Conclusions Congenital clubfoot deformities begin at early developmental stage and become exaggerated as the fetuses mature. The scoring system can quantify the extent of congenital clubfoot deformity and facilitate its management.     

论教学医院后勤管理创新

教学医院后勤工作是教学医院系统中的支持系统。旨在对医院医疗、科研及教学设备和设施的保障。医院后勤社会化的展开与发展,将使其中相当多的工作纳入社会的经营。然而,后勤作为医院工作的重要内容,仍应是一个有机的系统。教学医院后勤管理,就是围绕医院的任务和工作目标,对这     

先天性马蹄内翻足动物模型胚胎后肢(芽)评分系统的建立

Objective Quantification of the severity of congenital clubfoot is essential for its re-search and treatment. Up to date, no scoring system is available for hind limb bud development. In this study,a scoring system was established based on a murine model Methods Sixty female Wistar rats weighing 220～250 g were randomized into the six control groups and six experimental groups. Vaginal sperm plug detection day was considered to be day 0 of gestation. Hind limb buds from fetuses of E13, 14, 15,16,17 and 18 days were removed and processed. Results Morphogenetic differentiation changes were detected in the experimental groups. According the new scoring system, clubfoot-like deformity was present in 80.9% of the experimental fetuses. We were able to quantify the extent morphogenetic diffcrentiation of the hind limb accordingly. Conclusions Congenital clubfoot deformities begin at early developmental stage and become exaggerated as the fetuses mature. The scoring system can quantify the extent of congenital clubfoot deformity and facilitate its management.     

带蒂上唇黏膜瓣行鼻腔缩窄术治疗萎缩性鼻炎12例分析

目的：探讨治疗萎缩性鼻炎的有效方法。方法：使用带蒂上唇黏膜瓣行鼻腔缩窄术。结果：治疗12例24侧,近期疗效达100％。结论：带蒂上唇黏膜瓣行鼻腔缩窄术治疗萎缩性鼻炎操作简便易行,手术成功率高,效果显著。