皮肤病摄影的回顾和展望

医学知识最早由文字或绘画记载,皮肤病学亦是如此,已知最早被绘画记载的皮肤病是梅毒.很多著名皮肤病学家如Ferdinand Hebra(1816-1880年,Vienna皮肤病学校创立者)、Moritz Kaposi(1837-1902年,最早研究红斑狼疮、扁平苔藓等疾病,并命名Kaposi肉瘤)等都对皮肤病绘画有较高造诣.摄影是用光线凝固历史的艺术.与文字和绘画相比,摄影因“凝聚”真实影像元素而记载人类视觉,是记录医学资料的可靠方式.皮肤病学高度依赖直观表现,是最早应用摄影术的临床学科,并形成“皮肤病摄影”这一特殊领域.皮肤病摄影对临床诊疗、医学文献、专业教育、学术交流意义重大,成为现代皮肤病学发展的重要推动因素之一.     

汉族人抗核小体抗体与系统性红斑狼疮临床特征及其活动性的相关性

目的分析抗核小体抗体(AnuA)与系统性红斑狼疮(SLE)患者临床特征及其活动性的相关性。方法采用回顾性研究并用调查表的形式收集694例AnuA阳性患者临床资料,并与733例An-uA阴性病人进行比较分析。结果 AnuA与蝶形红斑、光敏感、关节炎、心包炎及狼疮性肾炎的发生相关(P<0.05);AnuA阳性可伴有其他自身抗体及免疫学指标异常出现;AnuA阳性与肾脏损害指标血尿、蛋白尿、管型尿及尿素氮的发生率明显相关(P<0.05);AnuA阳性与系统性红斑狼疮疾病活动性指数(SLEDA I)相关(P<0.05)。结论 AnuA可能是SLE的又一标志性抗体,其检测可用于疾病的早期诊断,有助于SLE疾病活动度、严重程度及狼疮肾炎(lupus nephritis,LN)的判断。     

氧化石墨烯负载多柔比星对多发性骨髓瘤细胞的杀伤作用

BACKGROUND: Cytotoxicity of graphene oxide to normal cells is relatively low, but whether graphene oxide loaded with doxorubicin has some effects on malignant cells is rarely reported. OBJECTIVE:To explore the cytotoxicity of graphene oxide loaded with doxorubicin on multiple myeloma cells. METHODS:Multiple myeloma cell line RPMI8226 in logarithmic phase was selected, cultured and divided into four groups, including graphene oxide loaded with doxorubicin, doxorubicin and graphene oxide groups as well as control group with no intervention. After 24 hours of culture, the cell activity was detected by cell counting kit-8 method, and the cell cycle and apoptosis were detected using flow cytometry. RESULTS AND CONCLUSION: Plump-shaped cells with translucent and clear cytoplasm were found in the control group; cells with relatively translucent cytoplasm, and even a few shrunken cells appeared in the graphene oxide group; cellular morphology was in a heterogeneity, apoptotic bodies appeared in the doxorubicin group; the cells was significantly reduced in size, presenting more obvious shrinkage and apoptotic bodies in the group of graphene oxide loaded with doxorubicin. The cell survival rate in the graphene oxide loaded with doxorubicin, doxorubicin and graphene oxide groups was significantly lower than that in the control group (P < 0.05), and this indicator was significantly lower in the group of graphene oxide loaded with doxorubicin than the graphene oxide group (P < 0.05). The apoptotic rate in the group of graphene oxide loaded with doxorubicin and doxorubicin group was significantly higher than those in the graphene oxide and control groups (P < 0.05), respectively. Additionally, there were no significant differences in the cell cycle among groups. These results show that graphene oxide loaded with doxorubicin has a stronger cytotoxicity, and can induce apoptosis in human multiple myeloma cells.     

MALDI-TOF MS技术在生物芯片检测中的应用

基质辅助激光解吸附电离/飞行时间质谱技术(MALDI-TOF MS)是近年来出现的一种精确分子量测定和结构分析的新型平台。以其高灵敏性、高准确度、分析速度快、测量范围宽、分辨率强、样品用量少等优点被广泛应用于检测多肽、蛋白质、核酸、多糖等生物大分子的分子质量和纯度、了解分子间相互作用及翻译后修饰、进行蛋白质和寡核苷酸测序以及高分子聚合物的分子质量分布等。     

先天性非大疱性鱼鳞病样红皮病分子遗传学研究进展

先天性非大疱性鱼鳞病样红皮病是一种常染色体隐性遗传病,主要由TGM1、ALOX12B和ALOXE3基因突变引起。近几年来先天性非大疱性鱼鳞病样红皮病的分子遗传学取得了很大进展,本文就TGM1、ALOX12B和ALOXE3基因定位、突变位点等作一阐述。     

白癜风遗传学研究进展

白癜风遗传学研究近年来取得了较大进展,应用血清学及分子生物学方法均发现HLA-Ⅰ、Ⅱ、Ⅲ类基因区与白癜风存在相关,其中以DRB4*0101相关性最为明显。另外在易感基因搜寻及易感区域定位方面也取得一定进展。     

EC耳脑胶治疗颅骨粉碎性骨折213例临床分析

分析总结我院1990年1月至2003年4月,采用EC耳脑胶对213例颅骨粉碎性骨折病人进行自体颅骨骨折碎片成形粘合复位术,收到满意疗效,现报告如下。     

Activator矫治安氏Ⅱ类错(牙合)前后髁突和关节盘位置改变的MRI研究

目的分析Activator功能矫治对髁突、关节盘的位置及盘髁关系的影响。方法选择20个AngleⅡ1错[牙合]患者,在斜矢状位闭口MRI影像上测量Activator功能矫治前后髁突和关节盘位置的改变。结果在无症状AngleⅡ1错[牙合]中有40％出现关节盘前移位,功能矫治前后其髁突和关节盘位置没有明显改变。结论Activator治疗不会改变髁突在关节窝中的位置,不会引起正常位置的关节盘前移位,也不会使已经存在的盘前移位复位。     

后入路腹腔镜肾囊肿去顶术20例报告分析

目的探讨后入路腹腔镜下肾囊肿去顶术的方法和术后疗效。方法20例均在全麻下用自制水囊建立后腹膜腔,距肾实质5mm用电钩或电剪切除囊壁。结果所有囊肿均顺利去顶,手术时间30～90min,平均35min,术中出血量10～80ml,平均30ml,术后住院3～10d,所有病理报告均为良性病变,随访1～12个月,20例未见囊肿复发。结论后入路腹腔镜肾囊肿去顶术安全、有效。     

脾区放疗治疗多毛细胞白血病1例并文献复习

0 引言 多毛细胞白血病(HCL)是一种少见的B细胞恶性克隆性疾病,伴有免疫球蛋白基因重排,表达B细胞表面分化抗原(CD19、CD20、CD22)和单克隆免疫球蛋白.该病进展缓慢,全血细胞减少,脾大,并常伴有骨髓纤维化等~([1-2]),其特征性免疫表型为CD11c、CD25和CD103阳性,抗酒石酸酸性磷酸酶染色(TRAP)染色联合骨髓和外周血形态学可以帮助诊断~([3]).