MicroRNA-370 directly targets FOXM1 to inhibit cell growth and metastasis in osteosarcoma cells

MicroRNAs (miRNAs) are endogenous, non-coding, small RNAs, which play a critical role in regulating varieties of the biological and pathologic processes. Several reports have indicated that miR-370 acts as a tumor suppressor in varieties of tumors. However, the roles of miR-370 in osteosarcoma have not been reported. In this study, our objective was to explore the biological functions and its molecular mechanism of miR-370 in osteosarcoma cell lines, finding a therapeutic target of osteosarcoma. Our data demonstrated that miR-370 was evidently reduced in osteosarcoma cell lines, whereas FOXM1 expression was markedly increased. Up-regulation of miR-370 suppressed proliferation, arrested cell cycle and induced apoptosis in osteosarcoma cells. Besides, invasion and EMT of osteosarcoma cells was also inhibited by introduction of miR-370. Next, we found that FOXM1 expression was significantly reduced by up-regulation of miR-370. Bioinformatics analysis predicted that the FOXM1 was a potential target gene of miR-370. Luciferase reporter assay further confirmed that miR-370 could directly target the 3’ UTR of FOXM1. Overexpression of FOXM1 in osteosarcoma cells transfected with miR-370 mimic partially reversed the effects of miR-370. In conclusion, miR-370 inhibited cell growth and metastasis in osteosarcoma cells by down-regulation of FOXM1.     

HPV-16 E6 promotes cell growth of esophageal cancer via downregulation of miR-125b and activation of Wnt/β-catenin signaling pathway

High-risk human papillomavirus (HPV) is a possible cause of esophageal cancer. However, the molecular pathogenesis of HPV-infected esophageal cancer remains unclear. The expression levels of some microRNAs including miR-125b have been negatively correlated with HPV infection, and miR-125b downregulation is associated with tumorigenesis. In addition, Wnt/β-catenin signaling pathway has been suggested to play an important role in esophageal cancer (EC). We examined miR-125b and Wnt/β-catenin signaling pathway in HPV-16 E6 promoted tumor progression in EC. HPV-16 E6 transfection decreased markedly the expression levels of miR-125b and promoted the colony formation in the Eca 109 and Kyse 150 cell lines, and restoration of miR-125b expression level antagonized the increased colony formation in HPV-16 E6 transfected cell lines. We also demonstrated that overexpression of E6 upregulated the Wnt/β-catenin signaling activity via modulating the multiple regulators including TLE1, GSK3β, and sFRP4. Overexpression of miR-125b restored the expression levels of these proteins. Expression of miR-125b was lower in HPV-16 E6 positive esophageal cancer tissues, and was negatively correlated with E6 mRNA levels. Our results indicate that HPV-16 E6 promotes tumorigenesis in EC via down-regulation of miR-125b, and this underlying mechanism may be involved in the activation of the Wnt/β-catenin signaling pathway.     

经内侧入路利用解剖钢板治疗股骨髁间骨折病例报告（英文）

股骨髁间骨折是一种常见的骨科疾病,通常由高能量暴力所致,如何选择合适的治疗方法是临床讨论的热点。经外侧入路利用解剖钢板治疗股骨髁间骨折是最常用的方案,经内侧入路治疗的报道较为罕见。股骨髁间骨折的内固定一般包括:微创内固定系统,动力髁间螺钉固定、克氏针固定、髁间钢板固定。该文报道的病例采用了胫骨近端解剖钢板经内侧入路治疗股骨髁间骨折,成功的固定了骨折,为治疗股骨髁间骨折提供了新思路。     

细胞块技术在恶性浆膜腔积液组织来源及分子病理检测中的应用

目的 探讨细胞块免疫组化技术在恶性浆膜腔积液组织来源及分子病理检测中的应用价值。 方法 抽取 42 例患者 的恶性浆膜腔积液,分层离心获取肿瘤细胞并制作成细胞蜡块,应用 HE 染色、免疫组化染色、基因测序等方法对其进行检测。 结果42 例患者中,利用恶性浆膜腔积液细胞块明确组织分型及组织来源 40 例,其中肺癌 29 例（腺癌 24 例、鳞癌 3 例、小细胞癌 2 例）,恶 性淋巴瘤 2 例,胃肠道癌 4 例,乳腺癌 4 例,卵巢癌 1 例。29 例肺癌样本中 24 例进行了肺腺癌基因检测,其中 9 例表皮生长因子受体 基因突变,包括 E19（del E746-A750）缺失突变 6 例和 E21（L858R）替代突变 3 例。 结论 细胞块技术对于恶性浆膜腔积液的诊断 有重要临床意义,有助于明确诊断并查找组织来源,同时能够进行组织分型及分子病理检测,有效指导肿瘤的分子靶向治疗。     

Correction: A highly selective fluorescent probe for human NAD(P)H:quinone oxidoreductase 1 (hNQO1) detection and imaging in living tumor cells

Correction for ‘A highly selective fluorescent probe for human NAD(P)H:quinone oxidoreductase 1 (hNQO1) detection and imaging in living tumor cells’ by Ya Zhu et al., RSC Adv., 2019, 9, 26729–26733.

The authors regret that some articles reporting probes for detecting human NAD(P)H:quinone oxidoreductase 1 were not cited in the original article. The missing references are listed below as ref. 1–6, and should be cited in the original paper at the end of the following sentence on page 26729:Herein, we designed and synthesized a novel fluorescent probe 1 for detection of hNQO1 based on TCF-OH as a chromophore and quinone propionic acid (QPA) as a recognition group.^1–6The authors sincerely apologise for this oversight.The Royal Society of Chemistry apologises for these errors and any consequent inconvenience to authors and readers.     

结节性多动脉炎累及冠状动脉的临床特点与诊治

<正>结节性多动脉炎(polyarteritisnodosa,PAN)是一种系统性坏死性血管炎,主要累及中小动脉,任何系统脏器的动脉均可受累。对PAN的诊断,目前均采用1990年美国风湿病学会(ACR)的分类标准[1]:(1)体质量下降≥4 kg(无节食或其他原因所致);(2)网状青斑(四肢和躯干);(3)睾丸痛和(或)压痛(并非感染、外伤或其他原因引起);(4)肌痛、乏     

浅析脐针右降四针

[目的]通过临床四则有效案例,探讨与总结右降四针的作用以及在临床上的作用与适应症。[方法]通过研读《脐针疗法》《易医解密》等书籍,指导右降四针在临床上的应用。通过治疗妇女围绝经期综合征、咽喉肿痛、中风后遗症、失眠等疾病的4个案例,总结右降四针在临床上的应用。[结果]齐永教授认为右降四针通过其进针方位的不同,能达到滋阴作用,在调理阴虚症状及治疗发热中能起到很好的作用。所举的以右降四针治疗妇女围绝经期潮热汗出、咽喉肿痛、中风所致的下肢运动不利及失眠的案例也取得显著疗效。[结论]右降四针具有滋阴、补肾、取降的作用,并且取穴、施术方便,给临床治疗提供了新的思路和方法,值得学习和推广应用。     

雷帕霉素对肥胖小鼠卵巢功能的影响

目的：研究雷帕霉素对母源性肥胖导致的卵巢功能异常的干预作用以及药物的安全性评估。方法：建立一种高脂饮食的雌性肥胖小鼠模型,然后随机分为注射雷帕霉素的处理组和注射PBS的对照组,24周后统计其排卵数目,收集卵母细胞进行纺锤体免疫荧光染色,以评估雷帕霉素对肥胖小鼠排卵功能及卵母细胞质量的影响,并通过蛋白免疫印迹分析和RT?qPCR分析检测卵巢相关基因在蛋白和mRNA表达水平的变化。结果：雷帕霉素处理的肥胖小鼠体重下降,其卵巢磷酸化核糖体蛋白（phosphorylated ribosomal protein 6,P?rps6）和磷酸化起始因子4E结合蛋白（phosphorylated 4E binding protein 1,P?4EBP1）的表达明显降低,且超排的卵母细胞数目与对照相比有增加趋势,但卵母细胞纺锤体染色结果显示卵母细胞质量无明显差异;处理后的卵巢肿瘤坏死因子（tumor necrosis factorα,TNF?α）的mRNA表达水平增加;血清中雌激素和促卵泡激素都无明显变化。结论：雷帕霉素有改善排卵功能的趋势,尚未发现对卵母细胞质量有明显影响,为未来研究雷帕霉素对肥胖女性生育力的影响提供了实验基础。     

黄芪甲苷通过促进小胶质细胞/巨噬细胞M2型极化抑制大鼠脑缺血后炎症反应

目的: 研究黄芪甲苷对大鼠脑缺血后小胶质细胞/巨噬细胞M1/M2极化及炎症反应的影响。方法: 将48只大鼠随机分为手术对照组、模型对照组和黄芪甲苷组。采用线栓法建立大鼠大脑中动脉阻塞模型。黄芪甲苷组造模后即刻腹腔注射黄芪甲苷（40 mg/kg）,随后1次/d,连续给药3 d。各组术后第1、3天采用改良的神经损伤严重程度评分（mNSS）和角试验进行神经功能评价;术后第3天采用2,3,5-氯化三苯基四氮唑染料（TTC）染色检测脑梗死体积;实时逆转录PCR检测M1型小胶质细胞/巨噬细胞表面标志物CD86、诱导型一氧化氮合酶（iNOS）和促炎因子TNF-α、IL-1β、IL-6的mRNA表达,以及M2型小胶质细胞/巨噬细胞表面标志物CD206、精氨酸酶1（Arg-1）、类几丁质酶3样分子1/2（YM1/2）及抗炎因子IL-10、TGF-β的mRNA表达;免疫荧光双标记法检测脑缺血周边区CD16/32/Iba1和CD206/Iba1的表达。结果: 与模型对照组比较,黄芪甲苷组mNSS分值降低、右转次数减少（P < 0.05或P < 0.01）,脑梗死体积减小（P < 0.01）,M1型小胶质细胞/巨噬细胞标志物CD86、iNOS、TNF-α、IL-1β和IL-6的mRNA表达下调（均P < 0.01）,M2型小胶质细胞/巨噬细胞标志物CD206、Arg-1、YM1/2、IL-10和TGF-β的mRNA表达上调（均P < 0.01）,脑缺血区CD16/32⁺/Iba1⁺细胞数量减少（P < 0.05）,CD206⁺/Iba1⁺细胞数量增加（P < 0.01）。结论: 黄芪甲苷对大鼠脑缺血损伤有保护作用,可能与促进小胶质细胞/巨噬细胞从M1型向M2型转化、抑制炎症反应有关。