相思子中相思豆碱的药理研究

本文报道相思子中相思豆碱对豚鼠实验性哮喘反应有抑制作用,对豚鼠过敏性休克有保护作用,能抑制磷酸组胺致大鼠皮肤血管通透性增加,但不能舒张支气管平滑肌。     

Expression of type-1 plasminogen activator inhibitor in the kidney of diabetic rat models

INTRODUCTION: Intrarenal coagulation and fibrinolysis are thought to be involved in the pathogenesis of diabetic nephropathy. However, gene expression of fibrinolytic factors in diabetic nephropathy has not been clearly defined. Therefore we determined the gene expression of fibrinolytic factors in the kidneys of diabetic rats. MATERIALS AND METHODS: As a model of type1 diabetes male Sprague-Dawley rats were used. They were divided into three groups: control, streptozotocin (STZ)-induced diabetic, and insulin-treated diabetic. Otsuka Long-Evans Tokushima Fatty (OLETF) rats were used as a model of type 2 diabetes; and Long-Evans Tokushima Otsuka (LETO) rats, as the control. Renal gene expressions of type-1 plasminogen activator inhibitor (PAI-1), tissue-type PA (tPA), and urokinase-type PA (uPA) were examined by real-time PCR. Localization of PAI-1 mRNA was investigated by in situ hybridization. RESULTS: Renal PAI-1 mRNA levels (versus control) were increased by 60-80% in STZ-induced diabetic rats (10 days or 3 weeks post STZ injection); and insulin treatment reduced this increased expression to the control level. In OLETF rats (38 weeks old), the renal PAI-1 mRNA level was 2.5-fold higher than that in age-matched LETO rats. Both tPA and uPA mRNA levels were significantly lower than those in LETO rats. PAI-1 mRNA was observed in intraglomerular cells and tubular epithelial cells of both models. CONCLUSIONS: Renal PAI-1 gene expression is up-regulated in both type 1 and type 2 diabetic rats, and changes in gene expressions of fibrinolytic factors may play important roles in the development and pathogenesis of diabetic nephropathy.     

高职院校贫困大学生心理健康现状及对策思考

对高职院校贫困大学生心理健康状况进行调查，结果发现：贫困大学生心理健康和一般大学生，即非贫困大学生相比。存在明显差异．贫困大学生患有心理向题的人数比例高于一般大学生。针对实际情况，提出相关对策，以提高贫困大学生的心理健康水平。     

食管癌APC/MCC、DCC基因杂合丢失的研究

目的研究ＡＰＣ（ａｄｅｎｏｍａｔｏｕｓｐｏｌｙｐｏｓｉｓｃｏｌｉ）、ＭＣＣ（ｍｕｔａｔｉｏｎｉｎｃｏｌｏｒｅｃｔａｌｃａｎｃｅｒ）和ＤＣＣ（ｄｅｌｅｔｅｄｉｎｃｏｌｏｒｅｃｔａｌｃａｎｃｒ）基因在人食管癌组织中的变化。方法采用聚合酶链反应（ＰＣＲ）及限制性片段长度多态现象（ＲＦＬＰ）法检测了４６例食管癌组织中ＡＰＣ、ＭＣＣ和ＤＣＣ基因的杂合缺失。结果ＡＰＣ、ＭＣＣ和ＤＣＣ位点的杂合性丢失的发生频率分别为２９．０％（９／３１）、３３．３％（８／２４）和３２．４％（１２／３７）。然而,这些基因位点的杂合性丢失与肿瘤病理学类型、肿瘤的大小、浸润性及转移性无统计学的显著相关性（Ｐ＞０．０５）。结论ＡＰＣ、ＭＣＣ和ＤＣＣ基因位点的杂合性丢失在一定程度上是食管癌基因改变的普遍现象,并可能在肿瘤的发生中起作用。     

针刺激发感传对颈动脉和椎动脉血流量作用的观察

本文介绍引用美国 Sramek 介绍的头部总血流量的测定方法,以针刺激发感传方法对主要以椎动脉供血不足的椎动脉型颈椎病55例,和颈动脉供血不足的缺血性脑血管疾病66例进行了临床疗效和实验观察,结果提示,气至病所组疗效最明显,有感传组次之,局感组相对最差。实验结果提示针刺激发感传后使血管痉挛得以缓解,降低了血管紧张度和阻塞程度,使颈动脉、椎动脉血流量明显增加,起到了通经脉、调气血、活血化瘀及改善脑循环的作用。     

Experimental study on ototoxicity of cisplatin

The ototoxicity of cisplatin and a comparison of the ototoxicity between cisplatin and kanamycin had been studied by morphological and biochemical methods. The results showed that the ototoxic effects of both drugs were similar, mainly affecting the cochlea, and next the vestibule. Lesions developed from the periphery to the center. The stria vascularis, the spiral ligament and the secretory epithelium of the vestibule were slightly injured by cis-platin. The K+ and Na+ concentrations of cochlear perilymph changed after the administration of both drugs. The ototoxicity of cis-platin appeared to be accumulated. So, while using cisplatin clinically, we stress the need for careful monitoring of the cochlear and vestibular functions.     

Peroxiredoxin Ⅲ在大鼠超负荷心肌肥厚过程中的表达变化

目的:探讨Peroxiredoxin Ⅲ(PrxⅢ)在大鼠超负荷心肌肥厚过程中的表达变化.方法:雄性SD大鼠随机分为假手术对照组和模型组,模型组大鼠实施腹主动脉缩窄术,制备压力超负荷心肌肥厚模型.分别于术后24h, 2、 4、 6周,测定血液动力学变化;称量心体质量;光镜和电子显微镜观察左心室的组织变化; 提取左心室RNA, RT-PCR分析PrxⅢ的表达变化.结果:术后24h两组大鼠的血液动力学和心肌形态无明显变化.术后2、 4、 6周时,模型组大鼠的SBP、 DBP、 LVSP、 LVED及左心室体质量指数逐渐增加,心肌纤维逐渐增粗,线粒体逐渐增多, 肌节增长.术后24h PrxⅢ的表达稍有降低,但无统计学意义,2周时则明显降低,4周有所上升但仍低于对照,6周时明显上升且高于对照组.结论:PrxⅢ mRNA表达呈现先降低后增高的趋势,即在心肌肥厚中早期表达降低,而在心肌肥厚形成后表达明显升高,表明PrxⅢ参与了超负荷心肌肥厚的发生.     

响应面法优化替考拉宁发酵培养基及其扩大培养

摘要：目的 通过响应面法,优化替考拉宁发酵培养基,并对发酵工艺参数进行优化,来提高其发酵产量。方法 以游 动放线菌TC19-3p-103为试验菌株,采用单因素试验确定发酵培养基考察因素的参考范围;利用最陡爬坡试验确定响应面试验 的中心区域;利用Box-Behnken响应面法,确定了发酵培养基中的有机氮源最佳浓度组合;并对起始搅拌转速与通气量这两个 发酵工艺参数进行单因素考察;在发酵过程中,采用流加技术控制碳源浓度。结果 经优化的发酵培养基,其摇瓶产量提高了 31.6%;50L罐发酵工艺参数优化后,发酵水平达到8558 mg/L。 结论 优化后的发酵工艺,显著提高了替考拉宁的产量,为其 工业化生产奠定了基础。     

Diaphragmatic hernia during pregnancy: a case report with a review of the literature from the past 50 years

Diaphragmatic hernia is a rare complication during pregnancy. Only 30 reports have been published on this subject in English between 1959 and 2009. Due to misdiagnoses and management delays, diaphragmatic hernia usually presents itself as a life-threatening emergency. Here, we present a case report of a patient with a traumatic diaphragmatic hernia who became acutely symptomatic during pregnancy. The diaphragmatic hernia was managed successfully, and we describe the presentation, management and outcome of this case. We also present a review of all of the reported cases of diaphragmatic hernias complicating pregnancy that have been published in English during the past 50 years.