亚麻籽胶的胶凝性质

采用流变学法测定了亚麻籽胶溶液的胶凝点、熔化点，并采用质构仪、扫描电镜和原子力显微镜等手段研究了影响亚麻籽胶凝胶强度的因素，结果表明亚麻籽胶具有胶凝性，它能形成一种热可逆的冷致凝胶，亚麻籽胶溶液的胶凝点低于其凝胶的熔化点，且亚麻籽胶溶液的胶凝点及其凝胶的熔化点均随冷却的起始温度的升高而升高。亚麻籽胶浓度、溶解温度、pH、NaCl、CaCl2及复合磷酸盐能影响亚麻籽胶的凝胶强度，亚麻籽胶的凝胶强度随着浓度的增加及溶解温度的升高而增强；在pH6～9的范围内，亚麻籽胶的凝胶强度达到最大；NaCl和复合磷酸盐可以降低亚麻籽胶的凝胶强度，低浓度（〈0．3％）的CaCl2可以增强亚麻籽胶的凝胶强度，而高质量分数（〉0．3％）的CaCl2能降低亚麻籽胶的凝胶强度。     

蛙皮素对PC-3细胞骨架及细胞内游离Ca~(2+)的影响

目的:观察蛙皮素(BBS)对前列腺癌PC-3细胞骨架形态及细胞内游离Ca2+([Ca2+]i)浓度的影响。方法:①利用免疫荧光法(IH),结合激光扫描共聚焦显微镜(LSCM)检测10-5mol/L浓度BBS处理的PC-3细胞角蛋白(CK)的表达,以反映其对细胞骨架形态的影响;②应用Fluo-3/AM荧光标记技术和LSCM检测不同浓度BBS(10-9、10-7、10-5mol/L)处理的PC-3细胞[Ca2+]i浓度。结果:①10-5mol/L浓度的BBS可促进PC-3细胞CK表达及伪足形成;②BBS可提高PC-3细胞[Ca2+]i浓度,并具有浓度依赖性。结论:实验证明一定浓度的BBS可明显提高PC-3细胞[Ca2+]i浓度及CK表达,进而影响PC-3细胞骨架形态。本研究为探索BBS应用于肿瘤研究以及BBS作用后细胞内信息传递途径提供了基础。     

Preliminary evaluation of vitiligo using in vivo reflectance confocal microscopy

BACKGROUND: Vitiligo is the most common pigmentary disorder with a global incidence from 0.1% to 2% in different geographical areas. Histopathology and histochemistry have shown the reduction of melanocytes in achromic patches, but microscopic changes of lesional and non-lesional skin are still not completely understood. Reflectance confocal microscopy (RCM), based on the different light reflectance index of cutaneous structures, allowed in vivo, en face microscopic evaluation of superficial skin layers with a resolution similar to skin histology. AIM: The purpose of this study was to evaluate RCM features of lesional and non-lesional skin of vitiligo patients. Moreover, re-pigmented areas were taken into consideration in order to evaluate melanocyte response to ultraviolet B (UVB) radiation. SUBJECTS AND METHODS: Sixteen patients of different phototypes affected by active non-segmental vitiligo and 10 controls were enrolled in the study. In vivo skin imaging was done using a commercially available RCM (Lucid, Vivascope 1500. Re-pigmented areas from 6 to 16 patients (after UVB narrow-band therapy) were also examined. RESULTS: Vitiligo lesions showed the disappearance of the bright rings normally seen at the dermo-epidermal junction. Moreover, non-lesional skin of vitiligo patients showed unexpected changes as the presence of half-rings or scalloped border-like features of the bright papillary rings. In re-pigmented areas after UVB narrow band therapy, the presence of activated, dendritic melanocytes was seen. CONCLUSIONS: Considering our results, and following further studies, RCM clinical applications could be used in the therapeutic monitoring and evaluation of the evolution of vitiligo.     

整合素β1在自身免疫性心肌炎小鼠心肌的表达

目的:观察整合素β1在自身免疫性心肌炎小鼠心肌的表达变化。方法:猪肌凝蛋白皮下注射免疫Balb/c小鼠,建立慢性自身免疫性心肌炎模型,于初次免疫后14、30和60d对心肌组织进行病理学检查,同时用激光共聚焦技术和间接免疫荧光法检测整合素β1在心肌的表达变化。结果:在初次免疫后14d,小鼠心脏有局部炎症出现,整合素β1在心脏血管周围的表达高于正常组（P<0.05）;在第30天,小鼠心脏炎性浸润更加明显,整合素β1在心肌的表达增强,显著高于正常组（P<0.05）;第60天,小鼠心脏炎症细胞减少,而纤维化则开始出现,整合素β1在心肌的表达最强（P<0.05）,且排列紊乱。结论:整合素β1在自身免疫性心肌炎的发生及发展过程中起着不同重要作用,整合素β可能参与自身免疫反应及心肌的重构。     

电镜观察透明质酸酶对人眼晶状体上皮细胞作用

目的：探讨透明质酸酶是否可以溶解晶状体囊膜上皮细胞及其作用机制。方法。取人眼超声乳化白内障摘除术中撕下的前囊膜，对半切开，一半立即置于37℃水浴箱中的2ml平衡液中。另一半置于37℃水浴箱中的2ml平衡液 1500IU透明质酸酶中。分别放置5、1O、15min，然后立即放入4％戊二醛中固定，送透射电镜检查。结果：置于平衡液中5、10、15min的前囊膜，囊膜表面的晶状体上皮细胞之间的细胞连接完整;而置于透明质酸酶中5，10、15min的前囊膜，其表面的晶状体上皮细胞之间以及细胞与囊膜之间的缝隙随时间延长而增大。结论:透明质酸酶可以破坏晶状体上皮细胞之间以及细胞与囊膜之间的连接，从而使晶状体囊膜上的上皮细胞易于被清除。     

吗啡成瘾性大鼠脑内核团神经细胞[Ca 2+]i变化的研究

目的 研究吗啡成瘾对大鼠脑内与成瘾相关核团神经细胞[Ca 2 ]i的影响.方法 将50只SD大鼠随机分成吗啡成瘾组和对照组,观察成瘾后的戒断症状;应用对Ca2 敏感的探针Fluo-3与Ca2 络合后被激光激发发出荧光的特性,利用激光共聚焦显微镜观察伏核、海马、内侧额前皮质神经细胞[Ca 2 ]i变化,并用图像分析软件进行处理.结果 吗啡成瘾大鼠脑内伏核、海马及内侧额前皮质神经细胞[Ca 2 ]i和对照组比较明显增高(P<0.01).结论 长期使用吗啡可明显增高伏核、海马和内侧额前皮质神经细胞[Ca 2 ]i.