抗真菌药物联合治疗侵袭性真菌感染的研究进展

近些年,侵袭性真菌感染的发病率逐年增加。由于临床上单药治疗侵袭性真菌感染的效果欠佳,特别是耐药真菌的感染,可选择的药物极少,导致相关疾病的发病率和死亡率仍很高,故抗真菌药物的联合治疗得到了广大学者的青睐。总结了抗真菌药物联合治疗侵袭性真菌感染的机制,综述了针对中枢神经系统、肺部、血液系统、其他部位侵袭性真菌感染的联合用药情况,为临床合理使用抗菌药物提供参考依据。     

罗颂平治疗多囊卵巢综合征不孕症经验撷萃

总结罗颂平教授治疗多囊卵巢综合征不孕症的临床经验。罗颂平教授认为多囊卵巢综合征不孕病变的关键为"肾-天癸-冲任-胞宫"生殖轴功能失调,肾虚是主要因素。治疗多囊卵巢综合征不孕症需首重调经,平衡气血阴阳,孕后应重视补肾健脾固胎。此外,罗颂平擅用膏方调治本病,且临证不忘对患者进行生活作息、饮食及情志方面的积极引导,临床疗效颇佳。     

促进康复动机的策略在卒中康复中的应用进展

<正>1动机的概念和意义动机是一个经常使用的概念,并被视为康复结果的一个重要决定因素^[1]。动机是社会认知理论^[2]和自我决定理论^[3],两种常见行为理论的经验衍生因素。世界卫生组织的《国际功能和残疾分类》(international classification of functioning,disability and health,ICF)将动机定义为一种全面心理功能——一种有意识或无意识的驱动力产生激励以促进行为的功能^[4]。ICF框架通过结构与功能水平、活动水平和参与水平来描述一个人的健康情况,     

桂千金子提取物对金黄色葡萄球菌体外抗菌作用及机制的初步研究

目的:探讨桂千金子提取物对金黄色葡萄球菌的体外抗菌活性及作用机制.方法:通过体外抑菌试验,测定桂千金子乙醇提取物及其乙酸乙酯、正丁醇萃取物对金黄色葡萄球菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC),比较标准菌株金黄色葡萄球菌(SA)及临床分离的金黄色葡萄球菌(MSSA)和耐甲氧西林金黄色葡萄球菌(MRSA)的抗菌活性大小.测定桂千金子乙醇提取物作用后MRSA碱性磷酸酶(AKP)活性、外流DNA/RNA大分子含量、胞内蛋白含量的变化,采用电镜观察MRSA细胞形态.结果:桂千金子提取物对3种供试菌株的抑菌圈直径均大于15mm,MIC为1.56～12.50mg/mL,MBC为3.13～25.00 mg/mL,抗菌强度排序为乙醇提取物≈乙酸乙酯萃取物＞正丁醇萃取物;与空白对照组比较,实验组AKP活性升高(P＜0.05),各实验组与空白组外流DNA/RNA大分子含量和胞内蛋白含量比较,差异均无统计学意义(均P＞0.05);电镜下MRSA菌体细胞变形,表面粗糙有颗粒附着.结论:桂千金子提取物对金黄色葡萄球菌及其耐药菌株均具有显著的抗菌活性,其抗菌作用可能与细胞壁结构破损致通透性发生改变有关.     

基于数据挖掘的中医药治疗卵巢早衰用药规律研究

目的:采用数据挖掘的用法研究中医治疗卵巢功能早衰的方药规律。方法:查阅并筛选中国期刊全文数据库、维普、万方等数据库近10年来中医药辨治卵巢早衰的期刊论文,使用中医传承辅助平台V2.5进行录入,分析用药规律及常用处方的药物特点。结果:共筛选出文献134篇,含方剂共157条,使用频次较多的前5味中药依序是:当归、熟地黄、菟丝子、山药、淫羊藿。归纳分析处方中药物出现的频次、常用药对,共挖掘出28条核心用药组合,14条新处方。结论:补肾益精法应贯穿于治疗卵巢早衰的始终,并根据临证的需要,辅以补血养肝、健脾宁心、疏肝调气、活血豁痰。     

罗颂平论治崩漏经验

罗颂平认为,崩漏的发生以阴虚为本,临证需重视望诊以测疾病盛衰变化。治疗上顾护气阴应贯穿始终,在急性出血期不可全然收涩止血,须辅以活血化瘀,且使用活血药时应慎用辛温性燥动血类药物,需选用守而不走之品,常用罗元恺经验方"二稔汤"加减以补气养血、固冲摄血。在出血停缓阶段应着重补肾治本,兼顾调理肝脾气血,常应用罗元恺经验方"滋阴固气汤";并创制调经养血膏方纠偏祛病。还强调对患者进行情志疏导,身心、药食同治。     

Effect of Sea Cucumber Enzymolysis Fermentation Liquid on immunosuppressed mice based on metabonomics北大核心CSCD

Aim To prepare the sea cucumber enzy¬molysis fermentation liquid (SCEFL) by enzymatic hydrolysis of protease and fermentation of probiotics and to investigate the effect of SCEFL on the immunosup-pression induced by cyclophosphamide in mice and to explore its mechanism by metabomic method. Methods The immunosuppressive model was induced by in-traperitoneal injection of cyclophosphamide. C57BL/6J mice were randomly divided into normal group, model group, Levamisole group, SCEFL groups (at low, medium and high doses). The pathological changes of spleen were observed by HE staining. The proportion of CD4+and CD8+T lymphocyte subsets and the pro-portion of CD4 /CD8 T lymphocyte subsets in peripheral blood were detected by flow cytometry. The con¬tents of IL-2 ( interleukin-2 ), IgG ( immunoglobulin G) and IgM (immunoglobulin G) in serum were detected by ELISA. The changes of endogenous metabolites in mouse serum were analyzed by UPLC-Q/TOF-MS metabolomics, and the related metabolic pathways were explored. Results SCEFL could improve the pathological changes of immunosuppressed mice, in¬crease the proportion of CD4 T cells and the proportion of CD4+/CD8+T lymphocyte subsets,and increase the contents of IL-2, IgG and IgM in serum. The immune function of mice was regulated mainly through six related metabolic pathways, including glyc-erophospholipid metabolism, sphingolipid metabolism, linoleic acid metabolism, a-linoleic acid metabolism, glycophosphatidylinositol-anchored biosynthesis and arachidonic acid metabolism. Conclusion SCEFL may ameliorate the immunosuppression of mice by regulating endogenous metabolic disorder. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

近十年中药外治婴儿湿疹研究进展

对现有的中药外治婴儿湿疹的文献进行整理、归纳,分析近十年来中药外治婴儿湿疹的研究进展,发现中药外治婴儿湿疹具有显著优势。研发方便显效的中药外治法是今后科研与临床研究的重点。     

外周联合中枢双靶磁刺激促进脑卒中运动功能障碍康复专家共识

<正>重复外周磁刺激(repetitive peripheral magnetic stimulation,rPMS)作为一种远隔神经肌肉刺激模式正在运用于临床，发挥双向的神经激活作用，其疗效也在临床研究中得到证实^[1]。rPMS常与重复经颅磁刺激(repetitive transcranial magnetic Stimulation,rTMS)联合使用，形成了外周联合中枢双靶点磁刺激^[2]。但目前rPMS及其与rTMS联合临床应用尚无统一认识，缺乏规范化。