The protective effect of L-Shikonin on LPS/D-GalN-induced acute liver injury in mice via inhibiting NF-κB inflammatory signaling pathway北大核心CSCD

Aim To investigate the anti-inflammatory effect of L-Shikonin ( SK ) on lipopolysaccharide ( LPS)-induced RAW 264. 7 macrophages in vitro and its protective effect on LPS/D-GalN-induced acute liver injury. Methods The mouse model of acute liver in¬jury was established in vivo experiments by LPS/D- GalN. The survival rate of the mice and the changes of liver and spleen indices in each group were examined. The levels of AST, ALT and AKP in serum and NO, superoxide dismutase ( SOD ) and malondialdehyde (MDA) in liver tissue homogenate were measured, and the histopathological sections of the liver of each group were observed by H&E staining. M I T colorimet- ric assay was used for cell viability in vitro experi¬ments, Griess method for the detection of NO content, RT-PCR assay and Western blot assay for examining the effect of levulinic acid on the expression levels of mRNA and related pathway proteins of pro-inflammato¬ry factors in LPS-induced RAW264. 7 cells. Results The results of in vivo experiments showed that L-SK significantly improved the liver and spleen indices, de¬creased AST, ALT and AKP levels in serum, de¬creased NO and MDA in liver homogenate, and in¬creased SOD activity in mice with acute liver injury. The results of in vitro experiments showed that L-SK significantly inhibited the mRNA expression of INOS, COX2, I FN-(3 and pro-inflammatory factors 1L-6, TNF-a and IL-10 in LPS-induced RAW264. 7 cells, and significantly inhibited the protein expression of IN¬OS, COX2 and the NF-kB signaling pathway. Conclu¬sions L-SK has good anti-inflammatory effects in LPS-induced inflammation in RAW 264. 7 cells in vitro. Il inhibits the protein expression of phosphorylated P65 and IKKaαβ in the NF-kB signaling pathway, thereby suppressing the anti-inflammatory effects in vitro and L- Shikonin has protective effects against acute liver injury in mice. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

Effects of berberine on colon dermal cell apoptosis in mice with ulcerative colitis based on JAK/STAT signaling pathway北大核心CSCD

Aim To analyze the effects of berberine on the apoptosis of colon epithelial cells and polymorpho-nuclear neutrophils ( PMNs) in mice with ulcerative colitis ( UC ) by regulating JAK/STAT signaling pathway. Methods The UC mouse models were established by dextran sulfate sodium ( DSS) method and were randomly divided into control group, UC group, low-dose, middle-dose and high-dose berberine groups and positive drug group ( mesalazine enteric-coated tablet group) . In addition, the mice were randomly di¬vided into UC group, high-dose berberine group, AG490 group, and high-dose berberine + AG490 group. Levels of serum tumor necrosis factor a (TNF-α) and interleukin 6 (IL-6) and colon epithelial cell apoptosis and PMN apoptosis were compared among the groups. Western blot was used to detect the expres¬sions of colon tissue apoptosis-related and JAK/STAT signaling pathway-related proteins. Results The lev¬els of serum TNF-α and IL-6, apoptosis rate of colon epithelial cell and protein expressions of Fas, FasL, Bax, caspase-3, p-JAK2/JAK2 and p-STAT3/STAT3 in each dose berberine group and positive drug group were significantly lower than those in UC group (P < 0.05), and the above indicators in berberine groups were reduced gradually (P <0.05) . The PMN apoptosis rate and Bcl-2 protein expression were significantly higher in each dose berberine group and positive drug group than those in UC group (P <0. 05) , and the two indicators increased gradually in berberine groups ( P < 0.05). AG490 could reverse the above effects of berberine ( P < 0. 05 ). Conclusions Berberine can inhibit the apoptosis of colon epithelial cell and promote the apoptosis of PMN in UC mice by regulating the JAK/STAT signaling pathway, and then play a role in the treatment of UC. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

corilagin protects non-alcoholic fatty liver disease in mice induced by high fat and high sugar diet via regulating AMPK-autophagy signaling北大核心CSCD

Aim To explore the effects of corilagin on non-alcoholic fatty liver disease induced by high-fat and high-sugar diet in mice via regulating AMPK-autophagy signaling. Methods Healthy 8-week-old male C57BL/6J mice were randomly divided into control group, model group and corilagin group. The mice of model group and corilagin group were fed with a high-fat and high-sugar diet for four weeks at the age of eight weeks. The corilagin group mice were also intraperitoneally injected with corilagin (20 mg • k g - 1 ), which was given once every 2 days for 4 weeks. The mice of the control group and the model group were given equal dose of normal saline. After modeling and administration, the mice were sacrificed and the liver weight recorded. The liver pathological changes of each group mice were assessed by HE staining, oil red O staining and Masson staining. The biochemical indexes in serum and liver tissue were detected by the ELISA kit. The p-AMPK and autophagy levels were detected by Western blot. Results The results showed that compared to control group, the liver weight of the model group increased, the AST and ALT levels in serum also significantly increased, there were a large number of fat vacuoles and severe lipid deposition and mild collagen fibrosis in liver, while the liver weight and TG level in liver significantly decreased, and the liver pathological changes were significantly improved after treated with corilagin. Western blot results showed the levels of autophagy related proteins such as Atg7 and Atg5 significantly decreased in the model group, and the p-AMPK level also significantly decreased. When treated with corilagin, p-AMPK and the autophagy levels were up-regulated. Conclusion corilagin can protect non-alcoholic fatty liver disease in mice induced by high fat and high sugar diet. The mechanism may involve increasing p-AMPK level and enhancing autophagy level in liver. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

Mechanism study of OVA-induced allergic asthma in mice based on lipid metabolomics北大核心CSCD

Aim To investigate the mechanism and search for potential biomarkers of ovalbumin ( OVA ) -induced asthma in mice base on lipidomics. Methods A BALB/c mouse model of asthma was prepared by OVA. TNF-α, IL-4, IL-10, IFN-γ levels in BALF and IgE level in serum were measured by ELISA. The inflammatory changes in mouse lung tissue were observed using HE staining. Lipid mediators ( LMs) in lung tissue and serum were quantified with UPLC-MS/ MS strategy. Results IgE level in serum and TNF-α, IFN-γ levels in BALF were higher (P <0.05) of asthmatic mice.Typical inflammatory manifestations were seen in lung tissue of asthmatic mice. A total of 57 lipid mediators were quantified with UPLC-MRM. LMs metabolic profiles differed significantly in serum and lung tissue between asthmatic and normal mice, 17 significantly different LMs were found in lung tissue and 6 LMs were found in serum, and the differential metabolites were produced through the cyclooxygenase (COX), lipoxygenase (LOX) and cytochrome P450 oxidase (P450) metabolic pathways. Conclusions OVA-induced allergic asthma can cause disorder of lip-id mediators, LMs and cytokines are involved in the occurrence and development of asthma. The differential LMs have potential research value as biomarkers for the development of allergic asthma. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

The immunosuppressive effect of gossypol in mice is mediated by inhibition of lymphocyte proliferation and by induction of cell apoptosis

Aim： To investigate the immunosuppressive effect of gossypol in mice both in vitro and in vivo. Methods： The in vitro effect of gossypol on the proliferation of lymphocytes isolated from lymph nodes of BALB/c mice was determined by CFSE staining and by an MTS assay. Lymphocyte activation and lymphoblastic transformation were evaluated with immunostaining. Cell apoptosis was detected by Annexin-V and Hoechst 33342 staining. The in vivo immunosuppressive effect of gossypol on the DTH reaction was evaluated using a mouse DTH model induced by 2,4-dinitro-1- fluorobenzene （DNFB）. The thickness of the ears was measured, and the histological changes of the mouse auricles were observed after hematoxylin-eosin staining. The proliferation capacity of lymphocytes from DTH mice was also assayed. Results： In vitro, gossypol could significantly inhibit the proliferation of mouse lymphocytes stimulated with phorbol ester plus ionomycin in a dose-dependent manner. Although the expression of the early activation antigen CD69 was not affected, the lymphoblastic transformation of both T and B lymphocyte subsets was significantly suppressed by gossypol. Moreover, gossypol could induce apoptosis of lymphocytes, and the effect was time- and dose-dependent. In vivo, the DTH reaction in mice was markedly alleviated by gossypol injected intraperitoneally. Lymphocytes from drug-treated DTH mice had a reduced proliferation capacity as compared with lymphocytes from untreated DTH mice. Gossypol treatment also markedly reduced the number of infiltrated lymphocytes in the auricles of DTH mice. Conclusion： Gossypol exhibited immunosuppressive effects in mice, probably by inhibition of lymphocyte proliferation and by induction of cell apoptosis.     

Inhibitory effect of combination of tanshinone I,metformin and aspirin on malignant melanoma model mice北大核心CSCD

OBJECTIVE: To explore the antitumor effects of combined tanshinone I (Tan I), metformin (Met) and aspirin (Asp) on malignant melanoma in mice and the possible mechanisms. METHODS: C57BL/6 mice were injected with 0.1 mL B16F10 cells (2.8×109 L-1) to establish the subcutaneous transplantation tumor model at the right forelimbs axillary. Then, the mice were divided into 8 groups according to body mass, including model group, Tan I group (20 mg.kg-1 ip), Asp group (210 mg.kg-1, orally in drinking water), Met group (70 mg.kg-1, orally in drinking water), Asp+Met group, Tan I +Asp group, Tan I +Met group and Tan I +Asp+Met group, 10 mice in each group. Each mouse drank about 7 mL of water every day for a total of 18 d. The mouse body mass was measured every other day and the tumor diameter was calculated every day. The mice were sacrificed after treatment, the tumor mass was measured and the tumor inhibitory rates were counted. The histopathological changes of the liver and spleen were observed with HE staining. The percentage of lymphocytes in the tumor tissue such as CD8T, CD4+T and Treg cells was detected by flow cytometry. Inflammatory factors such as interleukin-6 (IL-6), IL-1β and tumor necrosis factor-α (TNF-α) were detected by ELISA. RESULTS: The body mass (including tumor mass) of mice in different groups increased during the experiment, but that of Tan I + Asp+Met group increased more slower than in model group (P<0.01). At the end of the experiment, no lesions were seen in any liver or spleen tissue by pathological observation, and the number of survivors was 8/10 (model group), 8/10 (Tan I group), 7/10 (Asp group), 7/10 (Met group), 8/10 (Tan I +Asp group), 8/10 (Tan I +Met group), 7/10 (Asp + Met group) and 5/10 (Tan I +Asp+ Met group), respectively. Compared with model group, there were no obvious changes in tumor volume or tumor mass in Tan I, Asp and Met groups and other two-two joint groups, but the tumor volume and tumor mass in Tan I + Asp+ Met group were significantly decreased (P<.01, P<0.05), and the tumor inhibitory rate in this group was 46.2%. Compared with the model group, the percentage of CD8+T cells increased (P<0.05) in Tan I + Asp+ Met group, but there were no significant changes in other groups. The contents of IL-6, IL-1β and TNF-α in tumor tissue of Tan I +Met group were much higher than in model group (P<0.01, P<.05, P<0.05) and the content of IL-6 increased in Tan I +Asp+Met group (P<0.01). CONCLUSION: Combination of Tan I, Asp and Met can effectively inhibit the growth of melanoma in mice, which may be related to the increasing percentage of CD8+T lymphocytes and IL-6 in tumor tissue. However there are possibly some side effects. © 2017 Chinese Journal of Pharmacology and Toxicology. All rights reserved.     

Positive reaction of direct popliteal lymph node assay isn't induced in humanized NPG mice with reconstituted immune system北大核心CSCD

OBJECTIVE: To perform direct popliteal lymph node assay (d-PLNA) induced by D-penicillamine hydrochloride (D-pen) or streptozotocin (STZ) in humanized NPG (hu-NPG) mice with a reconstituted immune system, NPG mice and BALB/c mice respectively, and to compare the responses in these mice to elucidate the mechanism of d-PLNA. METHODS: Female NPG mice were irradiated and transplanted with human hematopoietic stem cells isolated from cord blood to form hu-NPG mice. The number and function of peripheral blood lymphocytes in hu-NPG mice were detected by flow cytometry and lymphocyte proliferation test. The hu-NPG, NPG and BALB/c mice were randomly divided into D-pen group and STZ group (5 mice per group), respectively. Then, the positive chemical D-pen (1 mg per mouse) or STZ (0.75 mg per mouse) was injected subcutaneously into the right hind footpad of these mice using the established d-PLNA protocol. On the 7th day after injection, the mice were sacrificed. The bilateral popliteal lymph nodes (PLNs) were weighed. The difference of d-PLNA response was evaluated at 7 d after the injection of positive drugs. RESULTS: All the NPG, hu-NPG and BALB/c mice behaved normally after the injection of positive drugs. No systemic toxicity was observed. The symptom of local irritation disappeared within 7 d. As in previous studies, the BALB/c mice showed a typical positive response of d-PLNA, as evidenced by the increase in both mass and cell count of the PLNs in the treated lateral (mass index >2 and cellularity index >5), while NPG mice and hu-NPG mice showed a negative resoponse. Pathological examinations demonstrated that the PLNs of NPG mice and hu-NPG mice had developmental defect in lymphoid tissue and were smaller in size than BALB/c mice. Phenotypic analysis of the peripheral blood lymphocytes in hu-NPG mice showed that the majority of human lymphocytes expressed B cell markers CD45+CD19+ (75.5±6.6)%, and only about (17.1±6.6)% of the lymphocytes expressed T cell markers CD45+CD3+. There were few lymphocytes in NPG mice. In addition, these cells did not proliferate with mitogen stimulation in the lymphocyte proliferation test. CONCLUSION: The d-PLNA reaction induced by D-pen or STZ in mice is closely related to the number and normal function of lymphocytes in vivo. Negative d-PLNA reaction results from a lack of normal lymphocytes in NPG mice or from defected human lymphocyte function in hu-NPG mice. Thus hu-NPG mice are currently not suitable for d-PLNA.     

Effect of Tanshitone on prevention and treatment of retinoic acid-induced osteoporosis in mice

Objective To observe the prevention and therapeutic effects of tanshitone(TAN)on retinoic acid induced osteoporosis in mice.Methods The mice osteoporosis was induced by given retinoic acid intragasttrically for two weeks.The histomorphological features of bone were observed and biochemical indexes in serum(Ca,P,ALP,TRAP,E2,BGP)were determined after mice were given TAN at the dose of 40,80,160 mg·kg-1 respectively.Results Tanshitone can induce high conversion of osteoporosis.The levels of P,ALP,TRAP and BGP in the TAN groups were lower than the model group,while the E2 level was higher than the model group.Conclusions Tanshitone can prevent the loss bone in the experimental mice.The mechanism may be that it improves the level of estrogenic hormone and inhibits the high bone turnover.     

Effects of lentinan on dendritic cell metabolism北大核心CSCD

Aim To study the effects of lentinan(LNT)on the metabolism of dendritic cells(DCs)by metabonomics, and uncover the potential mechanism of its regulation of DC function. Methods DC2.4 cells were co-incubated with LNT for 24 h, and the activity of the cells was detected by thiazolyl blue tetrazolium bromide(MTT)assay. The contents of interleukin-6(IL-6), tumor necrosis factorα(TNF-α)and interleukin-12(IL-12)in supernatant were detected by enzyme-linked immunosorbent assay(ELISA). The metabolic general changes of DC2.4 cells were detected by Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF/MS), and the differential metabolites were analyzed by multi-distance covariates and bioinformatics, partial least squares-discriminant analysis(PLS-DA). Finally, metabolic pathway analysis was performed by MetaboAnalyst 5.0. Results LNT did not significantly inhibit the activity of DC2.4 cells at the dose of 25～100 mg·L-1. LNT(100 mg·L-1)could significantly stimulate the secretion of IL-6, TNF-α and IL-12 in DC2.4 cells. 20 differential metabolites were identified in DC2.4 cells after being stimulated by LNT(100 mg·L-1), which involved 25 metabolic pathways including urea cycle, arginine and proline metabolism. Conclusion The regulation of LNT on DC function involves a variety of amino acid metabolism. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

Effect of a novel phosphodiesterase type 5 inhibitor,CPD1, on carbon tetrachloride-induced liver fibrosis in mice北大核心CSCD

Aim To investigate the effects of CPD1, a novel phosphodiesterase 5 inhibitor, on liver pathological phenotype and hepatic stellate cells (HSCs) activation in hepatic fibrosis model mice caused by carbon tetrachloride ( CCl4). Methods Male C57BL/6 J mice were divided into four groups randomly ( control group, CCl4group, CCl4+ CPD1 group and CCl4+ tadalafil group) . Hepatic fibrosis model was construc¬ted by intraperitoneal injection of CCl4( twice a week) . Four weeks after CCl4injection, the mice were treated with CPD1 (2 mg kg-1• d-1) , or Tadalafil (10 mg • kg-1• d-1) by intragastric administration, respec¬tively, for four weeks. Hematoxylin-eosin staining and Sirius Red staining were used to observe the distribu¬tion of liver tissue structural lesions and fibrosis. Im-munohistochemical staining was used to detect the ex¬pression of a-smooth muscle actin ( a-SMA) and fi-bronectin. Results Compared with control group, the liver tissue structure was seriously damaged in CCl4group with many hepatocytes necrosis and inflammatory cell infiltration, indicating that liver injury occurred in the CCl4-induced hepatic fibrosis model mice. Moreo¬ver, the expressions of a-SMA increased significantly in CCl4group. Compared to CCl4group, the liver tissue damage was significantly improved in PDE5 inhibitors group,most notably, CPD1 had a better curative effect than tadalafil did. Furthermore, CPD1 inhibited the ex¬pression of a-SMA markedly and reduced the expres-sion of ECM-related proteins induced by transforming growth factor pi ( TGF-f31 ) in Lieming Xu-2 ( LX-2 ) cells. Conclusions Phosphodiesterase 5 inhibitor CPD1 strongly alleviates CCl4-induced hepatic damage by inhibiting the activation of HSCs and expression of collagen fibers. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

Content of the anti-DNA antibody in the serum of BXSB mouse and suppressive effect of BXSB mouse serum on lymphocyte proliferation

The content of the anti-DNA antibody in BXSB mouse serum and the effect of the sere from BXSB mice on lymphocyte proliferation of normal mouse were observed.Enzyme-linked immunosorbent assay (ELISA) and ~3H-thymidine incorporation were used.The content of anti-DNA antibody in male BXSB mouse serum increased from the age of 3 month and reached to the     

Study on effect and mechanism of Celastrus Orbiculatus total terpenoids in regulating lipid metabolism

Objective To investigate the effect and mechanism of Celastrus Orbiculatus total terpenoids on lipid metabolism in hyperlipidemia mice.Methods ICR mice were selected as investigated subject.The hyperlipemia mice models were made with feeding high-fat forage and were randomly divided into six groups:the normal group,the model group,the positive control group(treated with simvastatin)and the three groups treated with Celastrus Orbiculatus total terpenoids with low,medium and high dosage,respectively.Each group included eight mice.The control group was fed normal forage,but other groups were fed high fat forage.All groups were allowed to drink water freely.Since the first day when the models were made,intragastric administration had been adopted.The normal group was fed normal forage without intragastric administration;the model group was received physiological saline 20 mL·kg-1·d-1 with intragastric administration;the positive control group received simvastatin 2.6 mg·kg-1·d-1 with intragastric administration;the three treated groups received Celastrus Orbiculatus total terpenoids 60 mg·kg-1·d-1,120 mg·kg-1·d-1,200 mg·kg-1·d-1 with intragastric administration respectively.Each group was weighed once a week.On the basis of establishing hyperlipidemia mice model,blood lipids,lipid metabolic enzyme,antioxidative capacity were investigated after 21 days feeding of high-fat forage.Results Compared with model group,TC and TG in mice treated with Celastrus Orbiculatus total terpenoids all reduced and HDL-C raised obviously(P<0.01).Celastrus orbiculatus total terpenoids was shown to decreased MDA content in both serum and liver,increased serum SOD activity and inhibited the activity of the cholesteryl ester transfer protein(CETP).Conclusions Celastrus Orbiculatus total terpenoids could remarkably modulate the lipid metabolic disorder in hyperlipidemia mice,and has a certain regulating function on lipoprotein,inferring that it could reduce the occur of atherosclerosis.The mechanism of regulating lipid metabolism might be related with decreasing the activity of CETP and increasing antioxidative capacity.     

番茄红素对寰枢椎失稳老龄小鼠记忆的影响

Objective To observe the effect of Lycopene on the improvement of memory abilities by cumulating lactic acid for long time in atlas dentata vertebrae senile mice. Methods Totally 30% lactic acid 30μL one time every week was injected into the place between the first and the second cervical vertebrae for 3 weeks. Forty mice were randomized into five groups:young control group, model group, VitE group(50 mg/kg), control group,and two therapeutic groups of lycopene which was intragastric at the doses of 5, 2.5 mg/kg once everyday. The changes of memory in mice were observed with water-maze test and step-down avoidance test. The activities of acetylcholine esterase(AchE), and the content of acetylcholine, (Ach) in encephalon were tested. HE staining in mice brain, including the cortex and hippocampus was demonstrated. Results Compared with model group. Lycopene high dose could significantly shortened the latency period and wrong times in water-maze test (P ＜0.01 ), In stepdown avoidance test, the reaction period was shortened significantly ( P ＜ 0.01 ) and the latency period was shortened significantly. The activities of ChE decreased (P ＜0.01 ) and the content of ACh increased in the Lycopene high doses group(P＜0.01 ). The therapeutic groups of lycopene had less pathological change of cellular necrosis,neuron loss in hippocampal CAI than the model group. Conclusion Lycopene has a certain protective and improving effect on the decline of memory ability and cervical lesion induced by lactic acid in atlanto-axial joint cumulating.     

番茄红素对寰枢椎失稳老龄小鼠记忆的影响

Objective To observe the effect of Lycopene on the improvement of memory abilities by cumulating lactic acid for long time in atlas dentata vertebrae senile mice. Methods Totally 30% lactic acid 30μL one time every week was injected into the place between the first and the second cervical vertebrae for 3 weeks. Forty mice were randomized into five groups:young control group, model group, VitE group(50 mg/kg), control group,and two therapeutic groups of lycopene which was intragastric at the doses of 5, 2.5 mg/kg once everyday. The changes of memory in mice were observed with water-maze test and step-down avoidance test. The activities of acetylcholine esterase(AchE), and the content of acetylcholine, (Ach) in encephalon were tested. HE staining in mice brain, including the cortex and hippocampus was demonstrated. Results Compared with model group. Lycopene high dose could significantly shortened the latency period and wrong times in water-maze test (P ＜0.01 ), In stepdown avoidance test, the reaction period was shortened significantly ( P ＜ 0.01 ) and the latency period was shortened significantly. The activities of ChE decreased (P ＜0.01 ) and the content of ACh increased in the Lycopene high doses group(P＜0.01 ). The therapeutic groups of lycopene had less pathological change of cellular necrosis,neuron loss in hippocampal CAI than the model group. Conclusion Lycopene has a certain protective and improving effect on the decline of memory ability and cervical lesion induced by lactic acid in atlanto-axial joint cumulating.     

Effect of Lulong Zaisheng Decoction II on chemotherapy-induced bone marrow suppression and its mechanism北大核心CSCD

Aim To investigate the effect of Lulong Zaisheng Decoction II on chemotherapy-induced bone marrow suppression in nude mice bearing colorectal cancer. Methods Male BALB/C nude mice were inoculated with human colon cancer cell HT-29 under the armpit. The tumor bearing nude mice were randomly divided into five groups: control group, chemotherapy group, positive drug group, Lulong Zaisheng Decoction II groups with high and low doses. The mice were given drugs by gavage once a day for 10 consecutive days. From the fourth day of the experiment, except for the control group, the nude mice were intraperitoneally injected with 5-FU at dose of 25 mg • kg-1 for 7 consecutive days. The mice in control group were injected with the same volume of normal saline. On the 11th day, blood was collecled from the orbit to measure peripheral hemogram. The mice were killed after cervical dislocation, then the morphology of bone marrow cells was observed - Bone marrow cell cycle, apoplosis rate and CD34+ were detected by flow cytometiy, mRNA expressions of granulocyle-macrophage colony stimulating factor (GM-CSF), granulocyte-macrophage colony stimulating factor receptor (GM-CSFR), inlerleukin-1 p (IL-1 (3) and inlerleukin-3 (IL-3) were tested by quantitative real-lime PCR, and the protein expressions of vascular endothelial growth factor (VEGF) and intercellular cell adhesion molecule-1 (VCAM-1) were tested by Western blot. Results Lulong Zaisheng Decoction II significantly elevated the number of white cells, increased the proportion of CD34 positive cells and the percentage of S cells, and reduced the apoplosis rate of bone marrow cells. Furthermore, it obviously up-regulated the mRNA expressions of GM-CSF, GM-CSFR, IL-1 (3 and IL-3, and promoted the protein expressions of VEGF and VCAM-1 in bones. Conclusions Lulong Zaisheng Decoction II can significantly alleviate bone marrow suppression caused by chemotherapy. Its mechanism is closely related to regulating cell cycle, preventing bone marrow cell apoptosis, promoting the secretion and expression of hematopoietic growth factors, and improving bone marrow hematopoietic microenvironment. © Food and Fermentation Industries. All rights reserved.     

过期妊娠外周血T淋巴细胞亚群和NK细胞的观察

Objective To study the expressions of T cell subsets and NK cells in peripheral blood of postterm pregnancy without symptoms of labor.And to explore the relationship between postterm pregnancy and maternal cell immune function.Methods The plasma levels of t cell subsets and NK cells were detected by using flow cytometry in 100 patients with postterm pregnancy and 100 patients with threatened labor from 37 weeks to 41 weeks.And their propo~ions were calculled with the WBC of blood routine.Results The proportion of NK cell,CD3+,CD8+in the experimental group were lower than those of the control group.There is no significant difference(P＞0.05)between them;CD4+,CD4+/CD8+are significantly lower than those of the control group(P＜0.05).Conclusion The immune function of the patients with postterm pregnancy is significantly lower than that of the normal labor.     

过期妊娠外周血T淋巴细胞亚群和NK细胞的观察

Objective To study the expressions of T cell subsets and NK cells in peripheral blood of postterm pregnancy without symptoms of labor.And to explore the relationship between postterm pregnancy and maternal cell immune function.Methods The plasma levels of t cell subsets and NK cells were detected by using flow cytometry in 100 patients with postterm pregnancy and 100 patients with threatened labor from 37 weeks to 41 weeks.And their propo~ions were calculled with the WBC of blood routine.Results The proportion of NK cell,CD3+,CD8+in the experimental group were lower than those of the control group.There is no significant difference(P＞0.05)between them;CD4+,CD4+/CD8+are significantly lower than those of the control group(P＜0.05).Conclusion The immune function of the patients with postterm pregnancy is significantly lower than that of the normal labor.     

Effect of γ-ray on metabolic enzyme CYP3A1 in rat liver on multiple levels北大核心CSCD

Aim To explore the effect of γ-ray on the mRNA,protein expression levels and metabolic activity level of the key drug metabolic enzyme CYP3A1 in rat liver. Methods Wistar rats were randomly divided into control group, 24 h post-radiation group and 72 h post-radiation group. The experimental group was exposed to total body irradiation of single 6 Gy γ-ray. Blood was collected from the orbital venous plexus for blood routine examination and biochemical analysis 24 h and 72 h after irradiation, and liver tissue was prepared for quantifying expression of CYP3A1 mRNA and liver-specific microRNA (miR-122-5p) through RT-PCR. The expression level of CYP3A1 protein was analyzed by Western blot, and the metabolic activity level of CYP3A1 detected by the specific substrate midazolam combined with LC-MS method. Results Com¬pared with the control group, the weights of the rats in the radiation group significantly decreased, and the number of white blood cells was markedly reduced. Simultaneously, the activities of alanine aminotrans-ferase and alkaline phosphatase continuously descended, as well as the levels of total bilirubin and bile acid significantly increased, which indicated that the liver may be damaged after radiation. The relative expression of CYP3A1 mRNA continued to increase significantly 24 h and 72 h after irradiation. CYP3A1 protein expression and metabolic activity levels showed an obvious increasing trend 24 h after irradiation, and rose significantly 72 h after irradiation compared with the control group. At the same time, the expression of miR-122-5p in liver of rats in the 24 h and 72 h post-radiation group continued to decrease rapidly compared with the control group. Conclusions γ-ray radiation may arouse damage effect on liver, which leads to the continuous up-regulation of the mRNA and protein expression levels of the capital metabolic enzyme CYP3A1 in liver tissue, as well as the elevation of the metabolic activity level. The regulatory mechanism might be related to miR-122-5p. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

Effect of acid sphingomyelinase on nonalcoholic fatty liver disease via PPARα- PGC-1α pathway北大核心CSCD

Aim To investigate the effects of acid sphingomyelinase(ASMase)on high-fat induced nonalcoholic fatty liver disease in mice and its regulation of PPARα- PGC-1α pathway. Methods ASMase knockout mice based on C57BL/6 background were constructed. Closed group heterozygotes were obtained through hybridized with wild-type mice(ASMase+/-),together with the littermate WT mice were prepared for NAFLD model in this study. The experiment was divided into four groups:WT+Chow:the WT mice were fed with normal diet for 12 weeks; WT+HFD:the WT mice were fed with high-fat diet for 12 weeks; ASMase+/-+Chow:the ASMase+/- mice were fed with normal diet for 12 weeks; ASMase+/- +HFD:the ASMase+/- mice were fed with high fat diet for 12 weeks. Biochemical method was used to detect serum TC,TG and liver TC,TG contents and liver function such as ALT and AST. Oil red staining,HE staining,Masson staining and Sirius red staining were performed to detect liver lipid accumulation,hepatocyte morphology and liver fibrosis. AmplexTM red sphingomyelinase kit was applied to detect ASMase activity. Western blot was performed to detect protein expressions of ASMase,PPARα,PGC-1α and CPT1. Results WT+HFD group displayed hypercholesterolemia and liver dysfunction. Levels of liver triglyceride(TG)were significantly higher than those in WT+Chow group(P<0.05 or P<0.01). Meanwhile,the hepatocytes showed marked steatosis,balloon-like changes,and fibrosis. Protein expression and activity of ASMase in liver increased significantly(P<0.01 or P<0.001),whereas CPT1,PPARα and PGC-1α expressions were not statistically significant compared with matched control group. Heterozygously ASMase-deficient mice reduced the elevated liver TG induced by HFD,as well as improving balloon-like changes and liver fibrosis. Furthermore,the expressions of PPARα,PGC-1α and CPT1 were up-regulated in ASMase+/- +HFD mice compared with WT+Chow group.Conclusions ASMase promotes hepatic steatosis and fibrosis,which may be related to its inhibition of PPARα-PGC-1α pathway. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

Effect of matrine hydrochloride on liver injury

Objective Searching the function that the Injection of the matrine hydrochloride prevents and cures acute chemical liver injury of mice、immunity liver injury of mice and chronic liver injury of rats.Methods Acute hepatic injury models of mice induced by Chemical poison carbon tetrachloride(CCl4),thioacetamide(TAA),D-galactosamine(D-GalN),immunity hepatic injury model of mice induced by BCG and fat polysaccharide(LPS),chronic liver injury model of rats induced by CCl4 were introduced in the experiment.The serum ALT and AST were measured in acute hepatic injury experiments.Serum ALT,AST,AKP,ALB,TP,BiL-T,creatinine,triglyceride,sialic acid,laminin,hyaluronic acid,type Ⅲ procollagen and type Ⅳ collagen,hepatic hydroxyproline(HyP)of rats in chronic liver injury animals were determined after Injection of the matrine hydrochloride.Results The Injection of the matrine hydrochloride reduced serum ALT and AST level of acute chemical liver injury of mice induced by CCl4,TAA and D-GalN.The index of the liver and the spleen of immunity liver injury of mice induced by BCG and LPS were decreased after the injection of matrine hydrochloride treatment.Compared with the model group,the injection may obviously inhibited serum ALT,AST,TP,AKP,TRI,BiL-T,creatinine,triglyceride,sialic acid,laminin,hyaluronic acid,type Ⅲ procollagen and type Ⅳ collagen activity of chronic liver injury of rats induced by CCl4,elevated ALB、A/G,reduced the liver HyP,decreased the index of the liver and the spleen.The liver visual observation,the pathology inspection and the HAI grading result showed the injection may reduce the inflammatory activity in liver tissue,restrain the liver cell damage,reduce the pseudolobuli formation.Conclusions The Injection of matrine hydrochloride had the protective function to acute chemical hepatic injury of mice induced by CCl4、TAA、D-GalN、immunity hepatic injury of mice induced by the BCG and LPS and chronic liver injury of rats induced by CCl4.