白花蛇舌草总黄酮的免疫调节作用

目的　研究白花蛇舌草总黄酮(FOD)的免疫调节作用。方法　通过测定小鼠脾细胞IgM抗体形成,脾淋巴细胞的增殖反应,小鼠血清IL- 2和IFN -γ含量以及观察对小鼠白细胞数量的影响,研究白花蛇舌草总黄酮的免疫调节作用。结果　白花蛇舌草总黄酮(15、30、60mg·kg-1 )促进免疫功能低下小鼠由ConA或LPS诱导的脾淋巴细胞的增殖反应,并提高小鼠血清IL 2和IFN -γ的含量;白花蛇舌草总黄酮(60mg·kg-1 )促进免疫功能低下小鼠脾脏IgM抗体形成,并升高抗肿瘤药物(环磷酰胺或阿糖胞苷)所致的小鼠白细胞减少。结论　白花蛇舌草总黄酮具有增强机体特异性免疫功能和非特异性免疫功能的作用。     

健脾升白合剂升高白细胞作用研究

目的:观察健脾升白合剂对化疗引起的白细胞减少症的预防和治疗作用.方法:以环磷酰胺腹腔注射造成小鼠白细胞减少症模型,用健脾升白合荆进行治疗,对模型小鼠外周血象和骨髓造血功能进行检测.结果:健脾升白合剂可明显对抗模型小鼠的白细胞减少.结论:健脾升白合剂可保护小鼠外周血中白细胞免受环磷酰胺的伤害.同时对白细胞的提高有一定的促进作用.     

白花蛇舌草总黄酮的抗炎及抗菌作用

目的观察白花蛇舌草总黄酮抗炎及抗菌作用。方法采用二甲苯诱导小鼠耳肿胀模型、大鼠松节油气囊肉芽增生模型、新鲜蛋清诱导大鼠足爪肿胀模型、醋酸所致小鼠毛细血管通透性增高试验和体外抗菌试验,观察白花蛇舌草总黄酮的抗炎及抗菌作用。结果白花蛇舌草总黄酮(15、30、60mg·kg-1)对二甲苯诱导的小鼠耳肿胀和醋酸所致小鼠毛细血管通透性增高有一定的抑制作用;白花蛇舌草总黄酮(12、24、48mg·kg-1)对大鼠松节油气囊肉芽增生和新鲜蛋清诱导大鼠足爪肿胀亦有明显的抑制作用;在体外,白花蛇舌草总黄酮对球菌和杆菌均具有不同程度的抑菌和杀菌作用,且对球菌的作用优于杆菌。结论白花蛇舌草总黄酮具有抗炎及抗菌作用。     

白花蛇舌草及其常见伪品的鉴别

目的:对中药材白花蛇舌草及其常见伪品伞房花耳草和纤花耳草进行性状和显微鉴别研究。方法:从外观性状、组织和粉末特征进行显微鉴别。结果:白花蛇舌草与伞房花耳草、纤花耳草在茎、叶、花、果实、形态、组织及粉末显微特征方面都有较大区别。结论:白花蛇舌草与其两个常见伪品的性状和显微特征有较明显的差异,可资鉴别。     

白花蛇舌草对鼠宫颈癌细胞抑制作用研究

目的 探讨白花蛇舌草对宫颈癌细胞的抑制作用.方法 对60只裸鼠建立裸鼠人宫颈癌细胞移植模型,当鼠宫颈癌肿瘤生长到1cm时将60只裸鼠随机分成两组,给药组与对照组各30只.给药组给予白花蛇舌草水煎液胃内灌药.观察比较两组荷瘤小鼠宫颈癌细胞的抑制率、小鼠的生存时间、HeLa细胞Ki-67抗原蛋白的表达率以及肿瘤组织凋亡上的差异.结果 给药组小鼠的生存时间较对照组生存时间长.白花蛇舌草对 HeLa细胞移植瘤生长有明显抑制作用,并可诱导HeLa细胞凋亡,Ki-67蛋白的表达下降( P＜0.05).结论 白花蛇舌草对宫颈癌细胞有抑瘤增殖和诱导、促进肿瘤细胞凋亡的作用.     

白花蛇舌草的化学成分及临床应用

目的为深入研究白花蛇舌草提供参考。方法查阅近几年来相关文献，对白花蛇舌草的研究进展进行综述。结果白花蛇舌草含有黄酮等多种化学成分，具有抗肿瘤、增强免疫功能、抗茵等作用，可用于治疗多种恶性肿瘤。结论白花蛇舌草具有广阔的开发前景。     

HPLC法测定复方白花蛇舌草胶囊中穿心莲内酯的含量

目的建立复方白花蛇舌草胶囊中穿心莲内酯的含量HPLC测定方法。方法用HPCL法对复方白花蛇舌草胶囊中穿心莲内酯进行定量分析。色谱条件采用Kromasil C18柱(250 mm×4.6 mm),甲醇—水(52∶48)为流动相,流速为:1.0 mL.min-1,检测波长为225 nm,柱温:30℃。结果穿心莲内酯浓度在11.40～68.40 mg.L-1范围内与峰面积值成良好的线性关系(r=0.999 2),回收率为96.46%,RSD=0.49%(n=5)。结论该法简便、准确,重复性好,可用于复方白花蛇舌草胶囊的定量分析方法。     

白花蛇舌草水溶性提取物的抗肿瘤作用和对化疗损伤的保护作用的研究

白花蛇舌草Hedyotisdiffusa是一种传统的抗肿瘤中药 ,可广泛应用于治疗各种肿瘤 ,从白花蛇舌草中提取出水溶性提取物 (H1 和H2 ) ,采用接种S 180的荷瘤小鼠灌服其 10天 ,观察H1 和H2 的抗肿瘤活性。H1 (12 5 ,6 2 5mg·kg- 1 )和H2(6 2 5 ,31 2 5mg·kg- 1 )能显著抑制小鼠移植性S 180实体瘤的生长 ,而且H1 和H2 与环磷酰胺合用 ,可以明显改善环磷酰胺所致的免疫器官萎缩和造血系统的损伤。     

白花蛇舌草抗肿瘤作用及机制最新研究进展

目的:综述白花蛇舌草抗肿瘤作用及机制的最新进展。方法:通过整理近年来国内外对白花蛇舌草抗肿瘤作用及机制的最新研究成果进行归纳总结。结果:现有文献表明白花蛇舌草在体内外均有抗肿瘤作用,其主要作用机制是诱导肿瘤细胞凋亡、影响其信号通路、增强免疫功能等。结论:白花蛇舌草的抗肿瘤作用已经得到研究者认可,但其作用机制还不完全明确。     

黄芪多糖对小鼠化疗中白细胞和骨髓有核细胞减少的影响

目的　探讨黄芪多糖对小鼠在化疗中白细胞和骨髓有核细胞减少的影响。方法　以环磷酰胺引起小鼠白细胞和骨髓有核细胞减少造模。常规计数外周血白细胞数量 ;取股骨测定骨髓有核细胞数量 ;同时采用ID50 法 ,观察黄芪多糖对小鼠环磷酰胺毒性的保护作用。结果　黄芪多糖对小鼠环磷酰胺毒性的小鼠存活率分别提高 2 0 % ,30 % ,5 0 % ,其中以 10 0mg·kg-1黄芪多糖保护作用最为明显 ,对小鼠骨髓有核细胞数和外周血白细胞数量分别提高 86 .8± 10 .4和 72 .1± 14 .2。结论　黄芪多糖对环磷酰胺致小鼠骨髓有核细胞数和外周血白细胞减少及其毒性有明显保护作用     

Effect of ZL-004 on raising leukocyte count

This study is to investigate the effect of ZL-004 on normal mouse and mice with leukopenia induced by chemotherapeutic agents. 5-Fluorouracil were administered intraperitoneally to mice to develop leucopenia, and the mice were treated with ZL-004. The number of peripheral leukocytes and the percentage of granulocyte in total WBC were examined. The results are that ZL-004 markedly raise peripheral blood leukocytes in the normal mice and the mice model of leukopenia. So, ZL-004 could protect mice against 5-fluorouracil damage and raise peripheral blood leukocyte. Features of bone marrow smears is myeloproliferative hyperactivity in the mice, particularly the matured granulocytic series were observed. The mechanism of ZL-004 is to act on the mouse bone marrow causing proliferation and differentiation.     

黄芪多糖与黄芪甲苷配伍对辐射损伤模型小鼠的保护作用

目的:研究黄芪多糖与黄芪甲苷配伍对辐射损伤模型小鼠的保护作用。方法:100只雌性ICR小鼠随机分为正常对照(等容生理盐水)组、黄芪多糖对照(80 mg/kg)组、黄芪甲苷对照(80 mg/kg)组、模型(等容生理盐水)组、黄芪多糖(80 mg/kg)组、黄芪甲苷(80 mg/kg)组与联合用药①、②、③、④(黄芪多糖80、40、20、80 mg/kg+黄芪甲苷20、40、80、80 mg/kg)组。灌胃给药,每天1次,连续14 d。末次给药后小鼠接受60Coγ射线(剂量:5 cy)一次性全身辐射以复制辐射损伤模型。测定小鼠30 d存活率和死亡小鼠平均存活时间;肝脏HE染色光镜下观察小鼠肝组织形态学;测定小鼠外周血白细胞数、骨髓细胞DNA含量、血清超氧化物歧化酶(SOD)活性。结果:与正常对照组比较,模型组小鼠30 d存活率降低,死亡小鼠平均存活时间缩短;小鼠肝细胞广泛肿胀,出现大滴性脂肪变、气球样变,肝脏细胞点状坏死严重,炎性因子大面积浸润组织;外周白细胞计数减少;骨髓细胞DNA含量减少;血清SOD活性减弱,差异具有统计学意义(P<0.01)。与模型组比较,联合用药①、②、③、④组小鼠30 d存活率升高,死亡小鼠平均存活时间延长,外周白细胞计数增加,骨髓细胞DNA含量增加,小鼠肝细胞形态学明显改善;联合用药①、②、③组小鼠血清中SOD活性增强,差异具有统计学意义(P<0.01)。结论:黄芪多糖与黄芪甲苷配伍是通过多靶点发挥作用,其中黄芪多糖与黄芪甲苷质量比为4∶1时配伍效果最佳。     

黄芪与红芪提取物对衰老模型小鼠脑组织抗氧化能力和海马结构功能的影响

目的 研究黄芪、红芪提取物的抗衰老作用。方法 采用D-半乳糖建立小鼠衰老模型,分别灌服同剂量黄芪与红芪提取物进行抗衰老实验。UV检测脑组织T-AOC、SOD活力和MDA含量,单细胞凝胶电泳检测脑细胞DNA损伤。光镜和电镜观察其脑组织学和海马超微结构的改变。结果 黄芪高剂量组,红芪中、高剂量组能显著提高衰老模型小鼠脑组织T-AOC、SOD活性,显著降低MDA含量,明显减轻DNA损伤,明显减轻海马神经元形态结构衰老。结论 黄芪、红芪提取物具有明显的延缓衰老作用,其作用机制可能与提高机体抗氧化能力,减轻自由基对脑细胞DNA的损伤,维护海马区神经细胞的结构完整性,改善或延迟脑组织和神经元退行性变有关。     

当归多糖与黄芪多糖配伍对辐射损伤模型小鼠的保护作用

目的：研究当归多糖与黄芪多糖配伍对辐射损伤模型小鼠的保护作用。方法采用60Coγ射线对小鼠进行全身照射复制辐射损伤模型。以小鼠30 d存活率和死亡小鼠平均存活时间、小鼠外周血白细胞数、骨髓细胞DNA含量、血清丙二醛（ MDA）含量为指标,观察当归多糖、黄芪多糖及其不同比例配伍对辐射损伤模型小鼠的保护作用。结果在当归多糖、黄芪多糖及其不同比例配伍的药物保护组中,小鼠30 d平均存活率升高,死亡小鼠平均存活时间延长,外周白细胞计数增加,骨髓细胞DNA含量增加,MDA含量降低。结论当归多糖、黄芪多糖及其不同比例配伍对辐射损伤模型小鼠均有一定的保护作用,其中以当归多糖∶黄芪多糖（3∶1）配伍的效果最佳。     

Induction of hemopoiesis by saenghyuldan,a mixture of Ginseng radix,Paeoniae radix alba,and Hominis placenta extracts

AIM：To examine the efficacy of Saenghyuldan and its components,Ginseng Radix ,Paeoniae Radix Alba,and Hominis Placentsa extracts (SHD,GR,PRA,and HP,respectively) on the hempopoiesis in a myelosuppression model system.METHODS:Susceptibility to cyclophosphamide(CP) and S 180 carcinoma was determined in SHD,GR,PRA,and HP-treated mice,Analysis of peripheral blood and bone marrow cells as demonstrated by changes in cell types and histopathologic examination.The expression of cytokine mRNAs involved in hemooiesis was examined by RT-PCR,RESULTS:SHD and its seperated components (GR,HP,and PRA ,respectively)significantly increased the survival in CP-and S 180-treated mice.The hematology data demonstrated that all the agents augmented monocyte and leucocyte counts in the peripheral blood and increased bone marrow density and the ratio of leukocyte to erythrocyte in the bone marrow.These findings were positively correlated with the u-regulation of cytokine mRNA expression such as granulocty colony-stimulating factor(GM-CSF),erythropoietin(EPO),thrombopoietin(TPO),stem cell factor (SCF),and c-Kit.CONCLUSION:SHD is an effective remedy for the bone marrow failure and myelosuppression occurring during chemotherapy.     

Investigation on the marker substances of crude drugs in formulations. I. Marker substances for the identification of astragali radix in kampo and drinkable preparations]

There are few reports about marker substances for the identification of Astragali Radix in formulations. First, constituents analysis was performed by HPLC for the screening of a marker substance, using several lots of Astragali Radix and its fluid extracts. As a result of the analysis, one of the main components was clearly detected even in the fluid extracts, and thought to be a good marker substance. This component was identified to be calycosin (7,3'-dihydroxy-4'-methoxyisoflavone) by the structural analysis. Then, the identification methods of Astragali Radix and its fluid extracts in formulations were investigated with an Astragali Radix extract as a reference standard solution and calycosin as a marker substance. This method was applied to three different kinds of Kampo formulations and three different kinds of drinkable preparations. Consequently, calycosin was clearly detected by a HPLC-multi wavelength detector system, in all investigated formulations. Calycosin will be successfully used as a marker substance for the identification of Astragali Radix and its fluid extracts in formulations.     

混合真菌多糖制剂对小鼠耐力及免疫功能的影响

研究混合真菌多糖制剂对小鼠耐力及免疫功能的影响。方法：混合真菌多糖制剂按４．０、８．０ｇ／ｋｇ连续灌胃给药 ７ ｄ,分别测定小鼠耐缺氧能力、抗疲劳作用、免疫器官称重以及网状内皮系统吞噬功能等。结果：混合真菌多数能明显提高小鼠的耐缺氧及抗疲劳能力,促进正常小鼠免疫器官重量增加,增加网状内皮系统的吞噬功能,并能使环磷酸胺所致免疫功能低下小鼠外周血白细胞增加,促进骨髓有核细胞增殖及增加脾指数。结论：混合真菌多精制剂具有增强机体免疫功能及升高白细胞等作用。     

黄芪口服液制备工艺的优选

目的优选黄芪口服液的制备工艺。方法采用正交设计法,考察提取时间、次数、加水量及壳聚糖在药液中的浓度等4个因素。以黄芪甲苷含量为指标成分。结果壳聚糖絮凝法可代替醇沉法用于黄芪口服液的澄清工艺。最佳制备工艺:选用壳聚糖在药液中浓度为0.07,澄清,加药材8倍量水提取3次,每次3h。结论本制剂稳定。测定方法重复性好。     

Change of the content of chemical constituents and anti-oxidative action of the decoction of radix ginseng combined with Flos Lonicerae, radix Polygoni Multiflori and Radix Astragali

Ginsenosides in the decoction of Radix Ginseng, Radix Ginseng with Flos Lonicerae, Radix Polygoni Multiflori or Radix Astragali have been investigated by high performance liquid chromatography (HPLC) and electrospray ionization mass spectrometric method (ESI-MS). Change of the content of ginsenosides was nonlinear in diverse combinative proportion of Radix Ginseng with Flos Lonicerae, while the stripping of ginsenosides was promoted by a small amount of Radix Polygoni Multiflori. In the combinative decoction of Radix Ginseng with Radix Astragali, ginsenosides contents were increased compared to single decoction of Radix Ginseng. Besides, ferric reducing antioxidant power (FRAP) method was developed for determination of the total antioxidative activity of n-butanol and water-soluble extracts from the decoction. The experimental results showed that antioxidative activity was better in the combinative decoction than that in single decoction, and the FRAP values of n-butanol extract were also greater compared with that of water extract.     

SELENATE- AND SELENOMETHIONINE-INDUCED LEUKOPENIA IN ICR FEMALE MICE

The investigations reported here were conducted to gain a better understanding of the comparative toxicity of selenium. Sodium selenate and selenomethionine were administered to young adult ICR female mice. Peripheral leukocyte counts and ratios of agranulocyte (Ag) to granulocyte (G) leukocytes were determined from blood that was collected at regularly scheduled intervals. Data indicated that both forms of selenium induced a transient yet marked decrease in the number of circulating leukocytes (leukopenia) following serial injections ( 2 mg Se/ kg/injection) . The leukopenia was more extensive and of a greater duration for selenomethionine-treated mice. For selenate-injected anim als the Ag/ G ratio increased sharply while the ratio shift for selenomethionine-injected mice was significantly less. Data are interpreted in regard to the different possible modes of action of selenate and selenomethionine on peripheral leukocytes causing leukopenia and the variable Ag/G ratio values.