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1.
布鲁生对小鼠非特异免疫功能的影响 总被引:1,自引:0,他引:1
目的:观察镇痛有效剂量的布鲁生(RU)对正常小鼠及Cyc所致免疫低下小鼠非特异免疫功能的影响。方法:测定小鼠碳粒廓清能力、免疫器官重量及外周血WBC,小鼠PMΦ吞饮中性红及产生IL-1能力。结果:测定小鼠碳粒廓清能力、免疫器官重量及外周血WBC,小鼠PMΦ吞饮中性红及产生IL-1能力。结果:Bru对正常鼠碳粒廓清能力、免疫器官重量、外周血WBC、PMΦ吞噬功能及产生IL-1能力仅有轻微促进作用(P 相似文献
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马钱子碱对小鼠淋巴细胞功能的影响 总被引:20,自引:1,他引:19
目的 观察镇痛剂量的马钱子碱( Bru) 对小鼠淋巴细胞功能的影响。方法 测定绵羊红细胞( S R B C) 致敏小鼠血清溶血素及脾抗体分泌细胞( A F C) 水平, 二硝基氯苯( D N C B) 所致小鼠迟发性超敏反应( D T H) ,以及丝裂原诱导小鼠脾淋巴细胞增殖和产生 I L 2 能力。结果 Bru(10 及20 mg·kg - 1 ,ip 显著增强小鼠对 D N C B 的特异性细胞免疫反应,但5 ,10 及20 mg·kg - 1 不影响对 S R B C 的特异性体液免疫反应。对环磷酰胺( Cyc) 降低的上述反应,3 个剂量的 Bru均有显著恢复作用( P< 005 ~001) 。 Bru 10 mg·kg- 1 ip增强 Con A 及 L P S 诱导的脾淋巴细胞增殖( P< 005) ,对 Cyc 所降低的脾淋巴细胞尤其 T 细胞增殖反应, Bru 5 ~20mg·kg - 1 范围内均使其恢复( P< 005 ~001) 。 Bru 01 ~100 mg· L- 1 在体外对丝裂原诱导小鼠脾淋巴细胞产生 I L 2能力无明显影响。结论 镇痛有效剂量的 Bru 对小鼠淋巴细胞具有功能依赖性的免疫调节作用 相似文献
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目的:研究β胡萝卜素(βCar)对电离辐射诱导的突变的影响.方法:T淋巴细胞克隆检测法测定大鼠T淋巴细胞次黄嘌呤鸟嘌呤磷酸核糖转移酶(HGPRT)位点的突变率和硫代巴比妥酸(TBA)法测定大鼠血丙二醛(MDA).结果:60Coγ375Gy照射大鼠显著提高HGPRT位点突变频率,igβCar10和20mg·kg-1,突变频率明显降低(P<005),应用βCar5mg·kg-1变化不甚显著,表现一定的剂量依赖性,同时βCar也明显降低60Coγ诱发的大鼠血中MDA水平(P<005).MDA下降水平与HGPRT位点突变频率降低呈明显正相关性(r=09978,P<005).结论:βCar具有抗电离辐射诱导的突变作用,且这一作用与其抗氧化作用有关. 相似文献
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目的:研究重组人内皮细胞衍生的白细胞介素-8(IL8)对失血性休克的作用.方法:大鼠股动脉放血至MABP532kPa,维持90min,复制晚期失血性休克模型.输血后,静脉注射IL8250μg·kg-1.放免法测定血浆ET1和6KPGF1α含量.结果:给予IL8后,MABP显著提高,休克状态改善,2h存活率相应提高;休克晚期血浆ET1水平比正常明显升高(21±4vs82±18ng·L-1,P<001),血浆6KPGF1α含量明显降低(107±12vs157±11ng·L-1,P<001).IL8显著降低血浆ET1水平(10±4ng·L-1,P<001),提高血浆6KPGF1α含量(368±16ng·L-1,P<001).结论:IL8具有较好的抗休克作用. 相似文献
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白三烯类化合物(Leukotrienes)对小鼠腹腔巨噬细胞生成白细胞介素6的影响 总被引:1,自引:0,他引:1
探讨了白三烯B4(LTB4)、白三烯C4(LTC4)及白三烯D4(LTD4)对小鼠腹腔巨噬细胞分泌白细胞介素6(interleukin6,IL6)的影响。用IL6依赖细胞株B9的MTT方法测定样品中IL6的含量。结果显示,LTB4能剂量依赖性地增加巨噬细胞培养上清液中IL6的含量。LTC4及LTD4促进巨噬细胞培养上清液中IL6含量的最适浓度分别为:69×10-8和805×10-8mol·L-1。结果提示:LTB4与LTC4及LTD4在某些生物学功能方面有一致性。与LTC4及LTD4相比,LTB4促进巨噬细胞培养上清液中IL6的含量的最适浓度则要大1~2个数量级,提示在炎症反应中,肽白三烯与IL6的相关性较强。 相似文献
6.
目的:检测过氧化氢(H2O2)、甲磺酸乙酯(EMS)、丝裂霉素C(MMC)、二甲基亚硝胺(DMNA)、苯并(a)芘(BaP)、2氨基芴(2AF)和环磷酰胺(CP)诱发小鼠、大鼠及人外周血淋巴细胞DNA单链断裂.方法:体外单细胞微量凝胶碱性电泳试验(慧星试验).结果:除EMS097mmol·L-1在小鼠淋巴细胞,MMC30μmol·L-1在小鼠、人淋巴细胞中呈阴性外,其余均为阳性.最低可检测浓度分别为H2O21μmol·L-1,EMS048mmol·L-1,BaP50μmol·L-1,CP20mmol·L-1,MMC10μmol·L-1,DMNA273mmol·L-1,2AF625μmol·L-1.CP、BaP、2AF需经S9Mix代谢活化才显示毒性.结论:彗星试验检测出MMC诱导大鼠,EMS诱导大鼠和人,以及H2O2、DMNA、BaP、CP和2AF诱导小鼠、大鼠和人外周血淋巴细胞DNA单链断裂损伤. 相似文献
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目的:研究转换酶抑制剂卡托普利(Cap)和依那普利拉(Ena)对SHR和WKY大鼠心肌细胞内游离Ca2+浓度的影响.方法:用荧光探针Fura2AM结合计算机图象处理技术测定分离心肌细胞内游离Ca2+浓度.结果:SHR心肌细胞内游离Ca2+浓度(174±5nmol·L-1)较WKY大鼠(148±15nmol·L-1)高(P<001).Cap和Ena能明显降低SHR心肌细胞内游离Ca2+浓度(分别为161±11和166±7nmol·L-1,P分别<005),但对WKY的无影响(P>005).两药均能明显降低NE和AngI引起的SHR和WKY大鼠心肌细胞内Ca2+升高,同时也能明显降低KCl引起的SHR细胞内Ca2+升高(P<005),但对WKY大鼠的无明显影响(P>005).结论:Cap和Ena对病理性电压依赖性Ca2+通道有直接抑制作用. 相似文献
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目的:研究阿司匹林铜(CuAsp)对血小板聚集性的影响及其机制.方法:用Born氏法测定CuAsp对兔血小板聚集性的影响.用荧光光度法和放射免疫法观察CuAsp对兔血小板5羟色胺的释放和TXB2的产生及血浆中TXB2和6ketoPGF1α水平的影响.结果:CuAsp体外呈浓度依赖性抑制花生四烯酸(AA)诱导的血小板聚集和5羟色胺的释放(IC50分别为17和19μmol·L-1,95%可信限为9-33和10-30μmol·L-1),且抑制TXB2的产生(P<005).CuAsp10mg·kg-1灌胃选择性抑制AA诱导聚集.降低血浆TXB2,同时升高6ketoPGF1α的水平(P<005).结论:CuAsp体内外均有比Asp更强的抗血小板聚集作用. 相似文献
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Drug dependence and abuse potential of tramadol 1 总被引:3,自引:0,他引:3
目的:在219例有曲马朵滥用史的阿片成瘾者中评价曲马朵的药物依赖性和滥用潜力.方法:采用阿片戒断症状量表(OWS)评价曲马朵身体依赖性;采用中文成瘾研究中心问卷(ARCICV)评价曲马朵精神依赖性;采用视觉类比量表(VAS)评价曲马朵的渴求程度.结果:曲马朵OWS各项戒断症状分值为005-107;ARCI测量药物欣快效应的MBG分量表,测量镇静效应的PCAG分量表和测量拟精神病效应的LSD分量表的平均值分别是73,61和34(F=3812,P<001);571%的曲马朵滥用者对曲马朵有不同程度的渴求(χ2=7586,P<001).结论:曲马朵在阿片成瘾者中具有较高滥用潜力. 相似文献
10.
目的:建立测定生长激素(GH)在体生物活性的方法.方法:以去垂体大鼠体重增长(BWG)和胫骨骺软骨板宽度(TEW)为指标,观察动物性别、给药途径、次数和周期不同对效应的影响;同时进行4dBWG,6dBWG和6dTEW法,测定GH的效价(平行线3×3设计).结果:♀和♂sc和im给药以及每日给药1次和2次的BWG和TEW差异无显著意义.给药6d比给药4d引起较大的BWG和TEW(P<005).4dBWG法和6dBWG法在0020-0500IU·d-1有较好的λ值(00660和01747)和r值(09000和09237);4dBWG,6dBWG和6dTEW法测得rhGH的效价为46132,39829和48023IU/amp.6dBWG法有较小的λ值和较低的ARFL值.结论:可在同一组去垂体大鼠体内同时用4dBWG,6dBWG和6dTEW法测GH活性,以6dBWG法较好. 相似文献
11.
Elaine S. Coimbra Rafael Carvalhaes Richard M. Grazul Patricia A. Machado Marcos V. N. De Souza Adilson D. Da Silva 《Chemical biology & drug design》2010,75(6):628-631
We report herein the condensation of 4,7-dichloroquinoline (1) with tryptamine (2) and D-tryptophan methyl ester (3) . Hydrolysis of the methyl ester adduct (5) yielded the free acid (6) . The compounds were evaluated in vitro for activity against four different species of Leishmania promastigote forms and for cytotoxic activity against Kb and Vero cells. Compound (5) showed good activity against the Leishmania species tested, while all three compounds displayed moderate activity in both Kb and Vero cells. 相似文献
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Zusammenfassung Mittels Gaschromatographie und Dünschichtchromatographie wiesen die Autoren 11 Substanzen nach, welche durch Injektion oder nach Verabreichung per os in die Kniegelenksynovialflüssigkeit eindrangen. In ihrer Aufstellung konnten sie eine direkte Beziehung zwischen Struktur sowie chemischphysikalischen Eigenschaften der Substanz und ihrer Fähigkeit, aus dem Blut in die Kniegelenksynovialflüssigkeit einzudringen, nicht nachweisen, außer der Tatsache, daß Substanzen mit starker Affinität zu Eiweißstoffen erst in höheren Dosen nachweisbar waren. 相似文献
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Lung disease and PKCs 总被引:1,自引:0,他引:1
The lung offers a rich opportunity for development of therapeutic strategies focused on isozymes of protein kinase C (PKCs). PKCs are important in many cellular responses in the lung, and existing therapies for pulmonary disorders are inadequate. The lung poses unique challenges as it interfaces with air and blood, contains a pulmonary and systemic circulation, and consists of many cell types. Key structures are bronchial and pulmonary vessels, branching airways, and distal air sacs defined by alveolar walls containing capillaries and interstitial space. The cellular composition of each vessel, airway, and alveolar wall is heterogeneous. Injurious environmental stimuli signal through PKCs and cause a variety of disorders. Edema formation and pulmonary hypertension (PHTN) result from derangements in endothelial, smooth muscle (SM), and/or adventitial fibroblast cell phenotype. Asthma, chronic obstructive pulmonary disease (COPD), and lung cancer are characterized by distinctive pathological changes in airway epithelial, SM, and mucous-generating cells. Acute and chronic pneumonitis and fibrosis occur in the alveolar space and interstitium with type 2 pneumocytes and interstitial fibroblasts/myofibroblasts playing a prominent role. At each site, inflammatory, immune, and vascular progenitor cells contribute to the injury and repair process. Many strategies have been used to investigate PKCs in lung injury. Isolated organ preparations and whole animal studies are powerful approaches especially when genetically engineered mice are used. More analysis of PKC isozymes in normal and diseased human lung tissue and cells is needed to complement this work. Since opposing or counter-regulatory effects of selected PKCs in the same cell or tissue have been found, it may be desirable to target more than one PKC isozyme and potentially in different directions. Because multiple signaling pathways contribute to the key cellular responses important in lung biology, therapeutic strategies targeting PKCs may be more effective if combined with inhibitors of other pathways for additive or synergistic effect. Mechanisms that regulate PKC activity, including phosphorylation and interaction with isozyme-specific binding proteins, are also potential therapeutic targets. Key isotypes of PKC involved in lung pathophysiology are summarized and current and evolving therapeutic approaches to target them are identified. 相似文献
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This study explored gender-related symptoms and correlates of alcohol dependence in a crosssectional study of 150 men and 150 women with a lifetime diagnosis of alcohol use disorders (AUD). Participants were recruited in equal numbers from treatment settings, correctional centres and the general community. Standardized measures were used to determine participants' use of substances, history of psychiatric disorders and psychosocial stress, their sensation seeking and family history of substance use and mental health disorders. Multivariate analyses were used to detect patterns of variables associated with gender and the lifetime severity of AUD. Men had a longer history of severe AUD than women. Women had similar levels of alcohol dependence and medical and psychological sequelae as men, despite 6 fewer years of AUD. More women than men had a history of severe psychosocial stress, severe dependence on other substances and antecedent mental health problems, especially mood and anxiety disorders. There were differences in family history of alcohol-related problems approximating same-gender aggregation. The severity of a lifetime AUD was predicted by its earlier age at onset and the occurrence of other disorders, especially anxiety, among both men and women. The limitations in the generalizability of these findings due to sample idiosyncrasies are discussed. 相似文献
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Class Cubozoa includes several species of box jellyfish that are harmful to humans. The venoms of box jellyfish are stored and discharged by nematocysts and contain a variety of bioactive proteins that are cytolytic, cytotoxic, inflammatory or lethal. Although cubozoan venoms generally share similar biological activities, the diverse range and severity of effects caused by different species indicate that their venoms vary in protein composition, activity and potency. To date, few individual venom proteins have been thoroughly characterised, however, accumulating evidence suggests that cubozoan jellyfish produce at least one group of homologous bioactive proteins that are labile, basic, haemolytic and similar in molecular mass (42-46 kDa). The novel box jellyfish toxins are also potentially lethal and the cause of cutaneous pain, inflammation and necrosis, similar to that observed in envenomed humans. Secondary structure analysis and remote protein homology predictions suggest that the box jellyfish toxins may act as α-pore-forming toxins. However, more research is required to elucidate their structures and investigate their mechanism(s) of action. The biological, biochemical and molecular characteristics of cubozoan venoms and their bioactive protein components are reviewed, with particular focus on cubozoan cytolysins and the newly emerging family of box jellyfish toxins. 相似文献
19.
Justin A. Tolman Nicole A. Nelson Stephanie Bosselmann Jay I. Peters Jacqueline J. Coalson Nathan P. Wiederhold Robert O. Williams III 《International journal of pharmaceutics》2009,379(1):25-31
Invasive pulmonary aspergillosis (IPA) is a fungal disease of the lung associated with high mortality rates in immunosuppressed patients despite treatment. Targeted drug delivery of aqueous voriconazole solutions has been shown in previous studies to produce high tissue and plasma drug concentrations as well as improved survival in a murine model of IPA. In the present study, rats were exposed to 20 min nebulizations of normal saline (control group) or aerosolized aqueous solutions of voriconazole at 15.625 mg (low dose group) or 31.25 mg (high dose group). Peak voriconazole concentrations in rat lung tissue and plasma after 3 days of twice daily dosing in the high dose group were 0.85 ± 0.63 μg/g wet lung weight and 0.58 ± 0.30 μg/mL, with low dose group lung and plasma concentrations of 0.38 ± 0.01 μg/g wet lung weight and 0.09 ± 0.06 μg/mL, respectively. Trough plasma concentrations were low but demonstrated some drug accumulation over 21 days of inhaled voriconazole administered twice daily. Following multiple inhaled doses, statistically significant but clinically irrelevant abnormalities in laboratory values were observed. Histopathology also revealed an increase in the number of alveolar macrophages but without inflammation or ulceration of the airway, interstitial changes, or edema. Inhaled voriconazole was well tolerated in a rat model of drug inhalation. 相似文献