微针阵列经皮促透NGF对家兔皮肤感觉神经再生的影响

目的探索NGF（Nerve growth factor,NGF）促进移植皮肤感觉恢复的微创给药方法。方法亚甲蓝染色经微针阵列处理后的离体兔皮,观察微孔的分布和排列,冰冻切片HE染色观察微孔的深度。兔皮片移植模型分别采用直接涂布、微针促透和皮内注射给药,ELISA法检测皮肤匀浆液中NGF的含量。皮肤组织切片免疫组化染色观察NGF的分布和神经纤维的再生情况。结果微针阵列处理后离体兔皮微孔排列均匀,深度可达真皮浅层。动物模型给药6h后,微针促透组和皮内注射组皮肤组织匀浆液中NGF含量分别高于直接涂布组5.48倍和6.45倍,差异均有统计学意义（P〈0.05）;NGF免疫组化染色显示,微针促透组真皮组织中有大量NGF存在,且分布均匀;动物模型给药30d后,皮肤组织S100免疫组化染色显示,微针促透组和皮内注射组真皮中可见再生的神经纤维,两组差异无统计学意义（q=2.36,P〉0.05）,并均显著高于直接涂布组（q1=7.74,q2=6.15,P〈0.05）。结论通过微针阵列可使NGF透过兔皮肤角质层到达真皮,促进兔移植皮肤感觉重建。     

醋酸曲安奈德利多卡因局部封闭注射治疗瘢痕疙瘩效果评价

目的探讨醋酸曲安奈德利多卡因局部封闭注射治疗瘢痕疙瘩的临床效果。方法随机提取经醋酸曲安奈德利多卡因局部的封闭注射治疗的175例瘢痕疙瘩患者临床资料,对其治疗效果、不良反应进行统计分析。结果醋酸曲安奈德局部封闭注射治疗瘢痕疙瘩3个疗程后,总有效率96％,随访6个月,有2例瘢痕体质者复发。结论醋酸曲安奈德治疗瘢痕疙瘩治疗次数少．操作简便。疗效好,不良反应轻微,具有临床应用价值。     

醋酸曲安奈德益康唑乳膏体外透皮吸收的评价试验

目的建立高效液相色谱(HPLC)法测定醋酸曲安奈德益康唑乳膏体外透皮接收液中药物的含量,验证自制与上市产品体外透皮吸收的一致性。方法采用色谱柱:辛烷基硅烷键合硅胶为填充剂(250mm×4.6mm,5μm);流动相:以0.094%无水己烷磺酸钠(水-乙腈-异丙醇)溶液为流动相A,以0.094%己烷磺酸钠(水-甲醇)溶液为流动相B,梯度洗脱;柱温40℃;检测波长227 nm;流速1.0 mL/min;进样量20μL。体外透皮实验采用改进的Franz立式扩散池,以实验用离体乳猪皮为透皮屏障,对比自制醋酸曲安奈德益康唑乳膏与市售乳膏的透皮效果,分别在一定时间点取样测定透皮接收液中醋酸曲安奈德和硝酸益康唑的浓度,计算其累积透皮吸收量、皮肤残留量及皮肤表面剩余量。结果自制乳膏和市售乳膏12h平均累计渗透量分别为11.1085μg/cm~2、11.7150μg/cm~2;透皮速率分别为1.3733μg/(cm~2·h)、1.4441μg/(cm~2·h);醋酸曲安奈德总体平均回收率分别为100.9%、99.9%;硝酸益康唑总体平均回收率分别为88.8%、86.8%,两者平均累计渗透量和总体平均回收率均无显著性差异(P0.05)。结论自制和市售乳膏在透皮吸收上具有一致性。     

高效液相色谱法测定醋酸曲安奈德尿素乳膏中醋酸曲安奈德的含量

目的:建立醋酸曲安奈德尿素乳膏中醋酸曲安奈德含量的HPLC测定方法.方法:用Suntek Kromasil-C8(4.6 mm×200 mm,5μm)色谱柱;流动相:甲醇-水(75:25);流速:1.0 ml/min;检测波长:240 nm;柱温:30℃.结果:醋酸曲安奈德在6.25～50.00μg范围内与峰面积呈良好的线性关系(r=0.999 9),平均回收率为99.60％.RSD=0.40％(n=9).结论:该法简便、准确、重现性好,可用于醋酸曲安奈德尿素乳膏中醋酸曲安奈德的质量控制.     

滚轮微针处理对复方利多卡因乳膏表面麻醉桡动脉穿刺疼痛的影响

目的观察滚轮微针处理对复方利多卡因乳膏表面麻醉后行桡动脉穿刺疼痛的影响。方法选取2012年1月至2012年12月上海市第七人民医院择期手术的老年患者60例,美国麻醉师协会分级Ⅰ或Ⅱ级。按照实施方法不同分为常规组(复方利多卡因乳膏表面麻醉1 h后桡动脉穿刺)和滚轮微针组(复方利多卡因乳膏涂抹再行滚轮微针处理后15 min桡动脉穿刺)各30例。采用疼痛视觉模拟(VAS)评分评估穿刺疼痛情况,统计穿刺总成功率和穿刺满意率,穿刺后24 h皮肤不良反应发生情况。结果滚轮微针组VAS评分低于常规组[(1.1±0.4)分vs(1.9±0.5)分,P<0.05],穿刺满意率显著高于常规组(93.3%vs 70%,P<0.05);两组穿刺总成功率和皮肤不良反应(发红,淤青)比较,差异无统计学意义(P>0.05)。结论复方利多卡因乳膏涂抹后行滚轮微针处理安全性好,可减轻桡动脉穿刺疼痛,提高患者的满意度。     

醋酸曲安奈德利多卡因局部封闭注射治疗瘢痕疙瘩效果评价

目的探讨醋酸曲安奈德利多卡因局部注射治疗瘢痕疙瘩的临床效果。方法选取2016年6月至2017年8月我院收治的瘢痕疙瘩患者100例,按随机数字表法将其分成实验组50例,对照组50例,实验组应用醋酸曲安奈德+利多卡因局部注射治疗,对照组行冷冻治疗。对比两组疗效。结果实验组瘢痕疙瘩治疗有效率为96.0%,高于对照组的56.0%,差异有统计学意义(P0.05)。结论醋酸曲安奈德利多卡因局部封闭注射治疗瘢痕疙瘩,效果满意,值得临床进一步借鉴、应用。     

醋酸曲安奈德益康唑乳膏透皮吸收试验

目的 建立醋酸曲安奈德益康唑乳膏体外透皮接收液中药物的HPLC测定方法,研究醋酸曲安奈德益康唑乳膏的体外透皮能力.方法 采用色谱柱:辛烷基硅烷键合硅胶为填充剂(5μm)(SP -120 -5 -C8 -AP);流动相:以0.094％无水己烷磺酸钠[水-乙腈-异丙醇(720:40:40)]溶液(85％磷酸调pH =3)为...     

醋酸曲安奈德在兔眼玻璃体腔不同部位注射后药物浓度比较

目的在结膜下、球后和玻璃体内3个不同部位注射醋酸曲安奈德悬浊液,比较其在兔玻璃体腔内药物浓度的变化。方法将醋酸曲安奈德悬浊液以玻璃体内、球后和结膜下3个部位注射入兔眼,分别于给药后1、2、4、8、24 h取出玻璃体样本,以紫外分光法测定醋酸曲安奈德浓度。结果三组中球后注射组与玻璃体内注射组在注射后1、2、4、8、24 h,两组的玻璃体内醋酸曲安奈德浓度比较差异均无统计学意义(P>0.05)。结膜下注射组与玻璃体内注射组在注射后1 h药物浓度比较差异无统计学意义(P>0.05),而在注射后2、4、8、24 h两组的药物浓度比较差异均有统计学意义(P<0.01)。结论球后和玻璃体内注射曲安奈德悬浊液在治疗眼后段疾病上是较为合适的给药方式。球后给药在剂量加倍后24 h内能维持相当的治疗效果,操作更简便,并发症风险更小。     

醋酸曲安奈德在兔眼玻璃体腔不同部位注射后药物浓度比较

目的 在结膜下、球后和玻璃体内3个不同部位注射醋酸曲安奈德悬浊液,比较其在兔玻璃体腔内药物浓度的变化.方法 将醋酸曲安奈德悬浊液以玻璃体内、球后和结膜下3个部位注射入兔眼,分别于给药后1、2、4、8、24 h取出玻璃体样本,以紫外分光法测定醋酸曲安奈德浓度.结果 三组中球后注射组与玻璃体内注射组在注射后1、2、4、8、24 h,两组的玻璃体内醋酸曲安奈德浓度比较差异均无统计学意义(P>0.05).结膜下注射组与玻璃体内注射组在注射后1 h药物浓度比较差异无统计学意义(P>0.05),而在注射后2、4、8、24 h两组的药物浓度比较差异均有统计学意义(P<0.01).结论 球后和玻璃体内注射曲安奈德悬浊液在治疗眼后段疾病上是较为合适的给药方式.球后给药在剂量加倍后24 h内能维持相当的治疗效果,操作更简便,并发症风险更小.     

丹皮酚泡囊乳膏的制备及其体外透皮吸收考察

目的 以泡囊为载体制备丹皮酚乳膏,优化丹皮酚乳膏的制备工艺及制备处方,并考察体外透皮吸收情况。方法 用乙醇注入法制备丹皮酚泡囊;用乳化法制备丹皮酚乳膏;应用控制变量法优化制备工艺;采用正交设计优化制备处方;并采用TP-6型透皮扩散实验仪,以大鼠腹部离体皮肤作为渗透屏障,通过高效液相色谱法测定药物的累计渗透量来考察丹皮酚乳膏体外透皮吸收情况。结果 基于丹皮酚泡囊制得的乳膏在12 h和24 h的累积渗透量分别是丹皮酚原料药的1.1倍和1.2倍,在24 h时皮内药物滞留量是丹皮酚原料药乳膏的1.26倍。结论 基于丹皮酚泡囊制得的乳膏在有效期内各时间段的体外透皮吸收高于原料药乳膏,且丹皮酚泡囊乳膏的皮内滞留量明显高于丹皮酚原料药乳膏。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.