CD3 AK细胞与IL－2联用治疗癌性胸腹腔积液的临床研究

目的探讨CD3AK细胞与IL－2联用对癌性胸腹腔积液的治疗效果。方法将24例癌性胸腹腔积液患者分为两组，研究组12例，应用CD3AK细胞与IL－2直接胸腹腔灌注，每周3次，连续2～3周；对照组12例，单用IL-2灌注治疗，疗程同研究组。结果研究组与对照组的有效率分别为66.7%和25%，两者比较有显著性差异(P<0.05)；研究组的细胞免疫功能较对照组明显提高(P<0.05)。结论CD3AK细胞与IL－2联用能有效地促进癌性胸腹腔积液的吸收，提高机体免疫功能，是治疗癌性胸腹腔积液的有效手段。     

结核、癌性胸水中可溶性白细胞介素2受体的测定及其意义

目的 :了解结核和癌性胸水中可溶性白细胞介素 2受体 (SIL - 2R)水平及其临床意义。方法 :用双抗夹心ELISA法测定 2 0例结核性和 15例癌性胸膜炎患者的胸水和血清SIL - 2R水平 ,同时用间接免疫荧光法检测其胸水T细胞亚群。结果 :结核和癌性胸水中SIL - 2R水平高于自身和对照组血清 (P <0 .0 5 ) ,结核性胸水SIL - 2R水平高于癌症组 (P <0 .0 5 ) ;结核性胸水中CD3 + 、CD4 + 、CD8+ 细胞的百分数高于癌性胸水 (P<0 .0 1) ;结核性胸水SIL - 2R水平与CD4 + 细胞的百分数呈直线正相关 (r =0 .6 2 87P <0 .0 5 )。结论 :结核性和癌性胸水中SIL - 2R水平差别具有显著性 ,胸水中SIL - 2R的测定对两组疾病的鉴别诊断具有参考价值 ;结核组胸水中SIL - 2R的升高反映了胸腔局部T细胞的活化     

OK-432胸腔给药治疗癌性胸腔积液的临床研究

目的 :观察OK 4 32治疗癌性胸腔积液的临床疗效及调节免疫功能的作用。方法 :癌症伴胸腔积液病人 32例随机分为 2组 :实验组为OK 4 32治疗组 16例 ;对照组为IL 2治疗组 16例。所有患者均经胸腔穿刺并埋置软管尽量放尽胸水 ,实验组OK 4 32总量 2 5KE ,分 3次注入胸腔 ;对照组IL 2总量 4 0 0万U ,分 4次注入胸腔。检测实验组患者治疗前后外周血CD3 、CD4 、CD8 、CD4 /CD8 、NK细胞水平 ,同时检测外周血血浆TNF α、IL 1β、IL 6。 结果 :按Miller标准 ,OK 4 32治疗癌性胸水总有效率为 81 2 5% ,IL 2治疗总有效率为 75% ,两组疗效差异无显著性 (Fisher精确概率P >0 .0 5) ;OK 4 32治疗后 ,患者外周血CD4 、CD4 /CD8 、NK细胞水平有显著性提高 (P <0 0 5) ,TNF α、IL 1β、IL 6均有显著性提高 (P <0 0 1或P <0 0 5)。结论 :OK 4 32治疗癌性胸水具有很好的疗效 ,OK 4 32可以改善肿瘤患者细胞免疫功能和增强细胞因子的表达。     

胸腹腔内灌注顺铂序贯微波热疗治疗癌性胸腹腔积液的临床观察

目的 观察腔内灌注化疗药物顺铂（DDP）联合热疗治疗癌性胸腹腔积液的疗效。方法 60例癌性胸腹腔积液病人随机分为两组，治疗组27例DDP胸腹腔内注入加热疗，对照组33例单用DDP胸腹腔内注入。结果 胸腹腔积液控制有效率治疗组为88．9％，对照组为60．6％，治疗组明显优于对照组（P＜0．05）。毒副作用无显差异（P＞0．05）。结论 DDP胸腹腔灌注化疗＋热疗有协同作用，能有效提高胸腹腔积液控制率，提高病人生存质量，值得临床推广使用。     

CIK细胞治疗恶性腹腔积液的临床疗效

目的:探讨细胞因子诱导杀伤(eytokine induced killer,CIK)细胞联合白细胞介素-2治疗恶性腹腔积液的临床疗效.方法:对65例患有恶性腹腔积液的患者实施引流术,研究组实施CIK细胞20ml配白细胞介素-2 100万U灌注,检测3天;对照组实施顺铂35 mg/m2和白细胞介素-2 120万U灌注,坚持四周,每周灌注一次.据影像分析四次灌注后的效果.结果:研究组30例患者,有效26例,痊愈15例;对照组35例患者,有效24例,痊愈14例.研究组的毒副作用显著小于对照组,P＜ 0.05.研究组的KPS评分显著高于对照组,P＜0.05.且病种不同,显效也有差别,以腹腔积液合并肺癌、乳腺癌、卵巢癌和结直肠癌患者的治疗效果比较好,治疗后KPS评分也较对照组明显升高,中等分组上升也较多,低分组很少,毒副作用以低热和皮疹为主.结论:腹腔灌注CIK细胞配白细胞介素-2治疗恶性腹腔积液的效果显著,毒副作用小,安全可靠,在提高患者生活质量方面效果明显.     

自身CD3AK细胞与拉米夫定联合治疗乙型肝炎的临床观察

目的 :探讨拉米夫定与CD3AK细胞联合治疗慢性乙型肝炎的临床治疗效果。方法 :将 73例慢性乙型肝炎患者随机分为两组。 5 8例患者接受CD3AK细胞治疗 (1疗程回输细胞总数为 1.1- 8× 10 10 )和每天服用拉米夫定 10 0mg ,15例每天单纯服用拉米夫定 10 0mg。结果 :73例患者经 12个月治疗后 ,拉米夫定和CD3AK细胞联合治疗组与单用拉米夫定治疗组HBVDNA转阴率分别为 86 .2 %和 80 .0 % ,无显著差异。但联合治疗组HBeAg阴转率、eAb转换率、ALT复常率显著高于单拉米夫定治疗组 (分别为 5 2 .6 %vs 14 .3% ,2 0 .7%vs7.1%和 97.2 %vs 84 .6 % )。联合治疗组CD3和CDST淋巴细胞绝对值在CD3AK细胞治疗后均增加在 85 %以上。结论 :拉米夫定联合自身CD3AK细胞过继免疫治疗乙型肝炎可有效提高患者HBeAg阴转率和ALT的复常率 ;能明显增强患者的细胞免疫功能 ;缩短拉米夫定对慢性乙型肝炎治疗疗程。     

CD3AK细胞治疗头颈部恶性肿瘤的近期疗效观察*

目的:观察CD3AK在头颈部恶性肿瘤的综合治疗中的效果.方法:81例头颈部恶性肿瘤分成CD3AK治疗组(n＝42)和对照组(n＝39),治疗组有术前、术后单用CD3AK细胞治疗,亦有术前化疗加用CD3AK细胞治疗;对照组有术前、术后单用LAK细胞治疗,有术前单用化疗.治疗前后检查肿瘤缩小情况,并检测外周血T细胞亚群和血清可溶性白细胞介素2受体水平.结果:CD3AK对舌癌的治疗效果最好,治疗组与对照组比较有显著性差异(P＜0.01);其次为喉癌与甲状腺癌,与对照组相比,有显著性差异(P＜0.05);再次为腮腺腺癌.各项免疫指标:CD3、CD4、CD8及CD4/CD8均有不同程度的提高;sIL-2R水平有所下降.结论:CD3AK细胞治疗作为新的肿瘤过继免疫治疗,在头颈部恶性肿瘤的综合治疗中,毒副反应较少,疗效满意,有很好的推广前景.     

细胞因子调节脐血CD3AK细胞诱导HL-60细胞凋亡

目的 :探讨重组肿瘤坏死因子α(rhTNF α,TNF α)、重组粒细胞集落刺激因子 (rhG CSF ,G CSF)对脐血CD3AK细胞诱导HL 6 0细胞凋亡的影响。方法 :用细胞形态学观察 ,DNA琼脂糖电泳 ,原位末端标记法检测HL 6 0 ,CD3AK细胞诱导的HL 6 0 ,TNF α诱导的HL 6 0 ,CD3AK与TNF α共同诱导的HL 6 0 ,CD3AK与G CSF共同诱导的HL 6 0进行检查分析。结果 :HL 6 0自然凋亡率 (4.6 0± 1.71) % ,;CD3AK细胞诱导的HL 6 0凋亡率 (15 .6 0±3.2 1) % ;TNF α(5ng/ml)诱导的HL 6 0的凋亡率 (5 .2 0± 2 .0 7) % ;CD3AK与TNF α共同诱导的HL 6 0凋亡率 (36 .70± 4 .4 0 ) % (F =94 ,P <0 .0 1)。CD3AK与G CSF共同诱导的HL 6 0凋亡率 (4.88± 2 .2 3) % (F =5 8,P <0 .0 1)。结论 :TNF α协同CD3AK细胞诱导HL 6 0凋亡 ;G CSF抑制CD3AK细胞诱导HL 6 0凋亡。     

CD3AK细胞液配合中药治疗原发性肝癌35例临床观察

目的 :探讨CD3AK细胞液配合中药治疗原发性肝癌的临床疗效及治疗应用价值。方法 :6 4例患者随机分两组 ,治疗组 35例应用CD3AK细胞液配合中药治疗观察 ,对照组 2 9例应用抗肝癌免疫核糖酸针剂配合中药治疗。结果 :治疗组与对照组总缓解率分别为 6 0 %、44 8% (P <0 0 5 )。治疗组与对照组生存质量变化总改善率分别为 82 9%、5 8 7% (P <0 0 1)。治疗组与对照组证候变化总改善率分别为 82 8%、6 2 % (P <0 0 1)。治疗后治疗组CD3 、CD4 、CD4 CD8 改善均比对照组显著 (P分别 <0 0 5、0 0 1)。结论 :CD3AK细胞液配合中药治疗中晚期肝癌能明显改善临床证候 ,具有良好的免疫调节作用 ,延长患者生存期     

癌性胸水肿瘤浸润淋巴细胞的生物学特性研究

目的 :探讨癌性胸水肿瘤浸润淋巴细胞 (TIL)的生物学特性 ,以便更好地应用于临床。方法 :用低浓度白细胞介素 2 (IL - 2 )诱导培养癌性胸水TIL ,按常规法计数TIL细胞增殖量 ,采用流式细胞仪分析TIL细胞表型及MTT法检测其杀伤细胞活性。结果 :经低浓度IL - 2 (10 0IU/ml)诱导培养TIL 2 1d后 :TIL增殖倍数为2 130± 1140 ,大于 10 0 0倍占 72 %。CD3 无显著性变化 (P >0 .0 5 ) ,CD4 、CD8有显著性差异 (P <0 .0 1) ,而CD4 /CD8比值高达 3.5 3± 0 .82 ,并且在 2 1d时TIL具有较高的细胞毒性。结论 :癌性胸水TIL体外诱导培养3周时TIL具有较强的生物活性 ,此时进行胸水治疗可取得可靠的疗效。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.