^14C—尿素呼气试验诊断HP感染临床价值探讨

为探讨非侵入性＾１４Ｃ－尿素呼气试验（ＵＢＴ）的临床诊断价值，２该法对６１例各种慢性胃病患者进行胃粘膜幽门螺杆菌（ＨＰ）感染状况检测，并与快速尿素酶试验（ＲＵＴ）、组织切片蓝染色（ＭＢ）进行比较，结果发现＾１４ＣＵＢＴ检测ＨＰＢ是性率为６．２％，显著高于ＲＵＴ（５０．１％）和ＭＢ法（４８．７％），＾１４Ｃ－ＵＢＴ检测ＨＰ诊断敏感性９１．７％、特异性７７％、阳性预测值７２．６％，阴性预测值９３．２％     

胶囊微量法^14C—尿素呼气试验诊断幽门螺杆菌感染

目的：建立胶囊微量法＾１４Ｃ－尿素呼气试验（＾１４Ｃ－ＵＢＴ）用于诊断幽直菌（ＨＰ）感染。方法：采用１μＣｉ＾１４Ｃ－尿素，胶囊给药，在收集５，１０，２０，２５，３０，４５，６０ｍｉｎ呼气样本的基础上建立了以＾１４ＣＯ２呼出高峰期为２５ｍｉｎ，直接用１６．６７ｍＢｑ／ｍｍｏｌ表示的检测方法，结果与ＨＰ细菌学培养及病理组织学比较。结果：共检测１３７例病人，ＨＰ阳性８３例，阴性５４例。以ＨＨＰ阴性第２     

^14C—尿素呼吸试验诊断幽门螺杆菌感染

目的分析比较１４Ｃ－尿素呼吸试验（１４Ｃ－ＵＢＴ）诊断幽门螺杆菌的准确性和实用性。方法以低剂量（３７ｋＢｑ）１４Ｃ标记的尿素作示踪剂，应用１４Ｃ－ＵＢＴ检查幽门螺杆菌（Ｈｐ）感染。结果以组织学培养阳性为标准，对４２例患者的试验结果表明：１４Ｃ－ＵＢＴ诊断符合率９５．２４％，假阴性４．７６％，与血清学方法无显著差别。结论１４Ｃ－ＵＢＴ检测Ｈｐ具有简单实用、敏感性和特异性高的特点，可作为临床常规应用的非介入性方法。     

^14C尿素呼气试验检测幽门螺杆菌感染

１４Ｃ尿素呼气试验检测幽门螺杆菌感染陈洁平徐采朴徐辉胡文华程绍钧柳凤轩王振华我们针对传统１４Ｃ－尿素呼气试验（１４Ｃ－ＵＢＴ）存在１４Ｃ－尿素剂量偏大［１８５～３７０ｋＢｑ）（５～１０μＣｉ）］、口腔尿素酶干扰等问题，就１４Ｃ－尿素剂量、给药方式等问...     

残胃对~(14)C-尿素呼气试验影响的研究

目的评估１４Ｃ－尿素呼气试验（１４Ｃ－ＵＢＴ）检测残胃幽门螺杆菌（ＨＰ）感染的准确性。方法残胃３２例及对照组４０例患者先用金标准（胃镜检查时于胃窦和胃体各取两块粘膜活组织，作快速尿素酶试验和常规病理切片５张，ＧＩＥＭＳＡ染色镜检ＨＰ）定性，再用１４Ｃ－ＵＢＴ检测ＨＰ。结果用金标准检测的残胃和对照组的阴性患者（分别为２０例和１４例），再用１４Ｃ－ＵＢＴ检测未出现假阳性，而残胃组的１２例和对照组的２６例阳性患者用１４Ｃ－ＵＢＴ检测各出现一例假阴性。两种方法检测的准确性无显著性差别（χ２＝０．３１５，Ｐ＞０．５）结论残胃对用１４Ｃ－ＵＢＴ检测ＨＰ的准确性无影响。     

^14C—尿素呼吸试验与组织学检查诊断幽门螺杆菌感染的对比研究

【目的】评估＾１４Ｃ尿素呼吸试验（＾１４Ｃ－ＵＢＴ）对幽门螺杆菌（ＨＰ）感染的诊断价值,并探讨胃粘膜组织学变化对实验结果的影响。【方法】取９６例因上腹症状行胃镜检查的患者胃窦粘膜活检,分别行改良Ｇｉｅｍｓａ染色和快速尿素酶（ＲＵＴ）试验,＾１４Ｃ－ＵＢＴ检查;将三项检查中两项（或以上）阳性者定为ＨＰ感染,分别对三种方法进行评价,并且观察其在不同胃粘膜病变中ＨＰ检出情况。【结果】＾１４Ｃ－ＵＢＴ的敏     

血清学与^13C—尿素呼吸试验检测反复腹痛儿童Hp感染比较

目的 探讨血清学及＾１３Ｃ尿素呼吸试验（＾１３Ｃ－ｕｒｅａ ｂｒｅａｔｈ ｔｅｓｔ,＾１３Ｃ－ＵＢＴ）在检测小儿反复腹痛（ｒｅｃｕｒｒｅｎｔ ａｂｄｏｍｉｎａｌ ｐａｉｎ,ＲＡＰ）幽门螺杆菌（Ｈｐ）感染中的应用及ＲＡＰ与Ｈｐ的关系。方法 采用＾１３Ｃ－ＵＢＴ及血清ＥＬＩＳＡ汉检测抗Ｈｐ－ＩｇＧ。结果 ２００例经＾１３Ｃ－ＵＢＴ检测有５８例检出Ｈｐ感染,检出率２９．４％（１５／５１）,２９．５％（３     

^14C—尿素呼吸试验在幽门螺杆菌根除治疗前后的检测价值

目的：观察短程瓣三联抗菌药物在十二指肠溃疡患者中的根除效果及＾１４Ｃ－尿素呼吸试验（＾１４Ｃ－ＵＢＴ）在治疗前后的检测价值。方法：５６例十二指肠溃疡患者，经内镜活检组织快速尿素酶试验，Ｗａｒｔｈｉｎ－Ｓｔａｒｒｙ染色及＾１４Ｃ－ＵＢＴ检查，二项阳性者诊断为ＨＰ感染。阳性患者给予奥美拉唑、克拉霉素及替哨唑治疗１周，疗程一１月及３月分别进行＾１４Ｃ－ＵＢＴ检测ＨＰ。结果：十二指肠溃疡患者ＨＰ感染率为９     

13C-尿素呼气试验诊断幽门螺杆菌感染的研究

目前国内诊断幽门螺杆菌（ＨＰ）的方法主要有组织切片染色、细菌培养和快速尿素酶试验等。这些方法虽然原理不尽相同，但都须经过胃镜获取胃粘膜组织标本后才能进行。关于１３Ｃ－尿素呼气试验（１３Ｃ－ＵＢＴ）与组织学比较的文献已有一些报道，但结果相差较大，一般认为其敏感性为７３％～９８％，特异性为８５％～１００％。本实验将１３Ｃ－ＵＢＴ结果与上述方法比较，评价用１３Ｃ－ＵＢＴ诊断患者感染的可靠性，现将结果报告如下。１对象和方法研究对象选取２０００年９月～２００１年４月具有上消化道症状患者４００例，女２４２例…     

苍南地区部分上消化道疾病患者幽门螺杆菌感染临床分析

幽门螺杆菌 (Ｈｐ)是慢性胃炎的主要致病因子、胃癌的重要致病因子 ,与消化性溃疡的发病密切相关。笔者通过对苍南沿海地区部分上消化道疾病患者Ｈｐ感染率的分析 ,以期了解该地区Ｈｐ感染的特点 ,为根除Ｈｐ作参考。１．１对象对１９９９年７月１日～２００１年４月１日因上消化道症状在我院接受胃镜检查的胃病患者 (１月内未服用过抗生素、铋剂和抑酸剂者 )１５５９例进行了Ｈｐ检测。１．２检查方法患者作胃镜检查当天空腹接受１４Ｃ -尿素呼气试验 (１４Ｃ -ＵＢＴ) ,并同时取胃窦粘膜做快速尿素酶试验。两项同时阳性者为阳性。１４Ｃ -…     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…