Changes of Immunoreactive β-Endorphin in Plasma, Pituitary and Hypothalamus of Rats during Oxygen-Induced Convulsions

We observed for the first time the differences of immunoreactive β-endorphin(IR -β- EP) content in plasma, pituitary and hypothalamus of rats under various conditionsusing radioimmunoassay (RIA) and the effects of naloxone and β - endorphin (β- EP) antiserumon initial time of convulsions (ITC), severity of convulsions(SOC) and mortality on surface(MOS) of rats to hyperbaric oxygen(HBO). The results suggest thatβ- EP may partici-pate in the course of oxygen - induced convulsions and be one of endogenous convulsion - causingagents.     

Molecular Weight of Cytoplasmic Malate Dehydrogenase,Mitochondrial Malate Dehydrogenase and Lactate Dehydrogenase of a Freshwater Catfish

The multiple molecular forms of cytoplasmic malate dehydrogenase(cMDH),mitochondrial malate dehydrogenase(mMDH)and lactate dehydrogenase(LDH)were studid in the liver and skeletal muscle of the freshwater catfish,Clarias batrachus.There were two electrophoretically distinguishable bands(AAand BB)of cMDH and mMDH which suggests that they are apparently encoded at two gene loci(A and B)in both the tissues.However,the presence of a single band(LDH-1)of LDH in liver and double bands(LDH-1and LDH-2)in skeletal muscle in which LDH-2 was predominant reflects the differentialexpression of LDH genes in different metabolic tissues to meet the requirement of energy production.The AA isoform(74kd)of liver cMDH was smaller than those o the AA form(110kd)of skeletal muscle.In contrast,the BB isoform of liver(42kd)and skeletal muscle(54kd)were more or less similar in size.Unlike the case of cMDH,the molecular weight of AA isoform(115kd)of liver mMDH was higher than those of the AA form(87kd)of skeletal muscle.Whereas the molecular weight of BB isoform(58kd)of liver was in proximity to the weight of BB form(44kd)of skeletal muscle mMDH.The size of AA muscle was larger as compared to AA form of mMDH in the liver(115kd) and skeletal muscle(87kd).But the size of BB isoform of both the isozyme was almost equal in these metabolic tissues.The molecual weight of liver LDH-1(96kd)was close to the weight of LDH-1(82kd)in skeletal muscle.The molecular weight of skeletal muscle LDH-2 was deduced as 37kd which is much more lower than the weight of LDH-1 in liver and skeletal muscle.The smaller size of LDH-2 in skeletal muscle may be of a physiological significance in this anaerobic tissue.     

汉英医药卫生人员学位名称

C产科硕士Master of Obstetrics(MAO)S兽医博士Doctor of Veterinary Medicine(DVM)产科学士Bachelor of Obstetrics(BAO)兽医学士Bachelor of Veterinary Science(BVSc)E耳科硕士Magister Chirurgiae Otologicae(MChOtol)T体育学士Bachelor of Physical Education(BPE)F放射学     

Enhanced oxidant stress in synovial vessels of patients on hemodialysis

Objective To test the hypothesis that the susceptibility of synovial vessels to redox uncoupling which could result in oxidative damage capable of promoting β(2)-microglobulin (β(2)m) deposition.Methods The expression of oxidative markers by microvascular endothelial cells in hemodialysis (HD) patients, non-uremic patients and normal subjects was determined using immunostaining and Northern blotting.Results Synovial vascular endothelial cells of HD patients exhibited evidence of oxidant stress: induction of malondialdehyde (MDA) epitopes and heme oxygenase. These oxidative markers were not detectable in microvascular endothelial cells of other tissues from HD patients, as well as in the synovial vessels of non-uremic or normal controls. Higher levels of heme oxygenase mRNA in synovia were seen in patients with β(2)m deposition compared with patients without β(2)m deposition. Conclusions Oxidative damage of the synovial microvasculature in uremic patients may be one of the factors which can promote deposition of β(2)m in osteo-articular tissue.     

噻萘普汀与碳酸锂对慢性应激抑郁模型大鼠海马pCREB表达的影响

Objective To research the effects of tianeptine and lithium on expression of pCREB in hippocampus of chronic stress depression rats. Methods All the experimental rats were divided by random into : Group of depression,Group of tianeptine,Group of lithium and Group of control. The rats of Group of depression, Group of tianeptine and Group of lithium were applied stress for 21 days,and meanwhile Group of control had no stress. The rats of Group of tianeptine were fed with tianeptine (50 mg/kg) , Group of lithium were fed with lithium (60 mg/kg) , while another groups were fed with normal sodium of the same volume. The ethology examination was performed by using method of open-field and experiment of fluid consumption. The expression of pCREB was detected by Western-blotting method. Results After the chronic stress,the horizontal crossing numbers,the erection times,the modification times and the percentage of sacchar-consumption of the rats of Group of depression were 23.2±23.0;8. 1 ±7.2; 3.6 ±3.5 and (55.4 ±11.7)% respectively, which were less than Group of control (46.0±18.9;20.3±11.3;8.4±2.7 and (68.5 ±8.2)% ; P＜0.01). The horizontal crossing numbers(28. 1 ±23.0) ,the erection times(12. 1 ± 9.4) and the modification times(5.5 ±3.2) of Group of tianeptine are less than those of Group of control (P ＜ 0. 05), but no significant difference compared with Group of depression; the percentage of sacchar-consumption(62.7 ± 10.6) % ,Group of tianeptine was more than Group of depression (P＜ 0.05 ) , but no obvious difference with Group of control. The horizontal crossing numbers, the erection times, the modification times and the percentage of sacchar-consumption of Group of lithium were less than those of Group of control (P ＜ 0.05), more than those of Group of depression but no significant difference (P ＞ 0.05). In Westernblotting method,the level of pCREB in the hippocampus of Group of depression was less than that of Group of control (P＜ 0.01); that of Group of tianeptine was more than that of Group of depression (P ＜ 0.01) but no obvious difference with Group of control; that of Group of lithium was less than that of Group of control (P＜0. 01) and more than Group of depression (P＜0.01). Conclusion Tianeptine could reverse the reduction of expression of pCREB in hippocampus of chronic stress depression rats and lithium partly did it.     

Study on insulin resistance and genetic polymorphisms in essential hypertension patients of two different kinds of TCM constitution

Objective:To investigate the relationship of insulin resistance and the polymorphisms of insulin receptor-related genes in essential hypertension patients of two different kinds of TCM constitution.Methods:Oral glucose tolerance test (OGTT) and insulin release test (InRT) were conducted in 217 essential hypertensive patients of either sluggish meticulous (SM) constitution (139 cases) or prosperous impetuous (PI) constitution (78 cases),and the polymorphism of three genes, including insulin-like growth factor-1 receptor (IGF-1R),insulin receptor substrate-1 (IRS-1) and 2 (IRS-2) genes were detected.Results:(1) OGTT,InRT and insulin resistance index (Homa-IR) were higher and insulin sensitive index (ISI) was lower in the patients of SM constitution than those in patients of PI constitution.(2) Significant difference of ISI and Homa-IR was shown in patients of both constitutions with genotype G of the 3 genes.Conclusion:Decrease of insulin sensitivity and increase of insulin resistance are more obvious in hypertensive patients with genotype G of the 3 genes of SM constitution than in those of PI constitution.Therefore,the difference in constitution might be one of the genetic characteristics for insulin resistance in hypertensive patients.     

汉英医药卫生人员学位名称

C产科硕士Master of Obstetrics(MAO)S兽医博士Doctor of Veterinary Medicine(DVM)产科学士Bachelor of Obstetrics(BAO)兽医学士Bachelor of Veterinary Science(BVSc)E耳科硕士Magister Chirurgiae Otologicae(MchOtol)T体育学士Bachelor of Physical Education(BPE)F放射学     

抑郁症患者快动眼睡眠特征及其与抑郁水平的相关性

Objective To explore the characteristics of polysomnography (PSG) of depressed patients and the correlation between rapid eye movement (REM) and severity degree of depression. Methods Polysomnography was used to assess patients'sleep condition and Montgomery-Asberg depression scale (MADRS) was used to assess the severity degree of depression. 90 patients and 30 healthy controls were included. Results Compared to healthy controls,sleep progress of depressed patients changed as follow:prolonged sleep latency((25.2 ±15.25) minutes) ,lowered sleep efficiency(0.853 ±0.11) ;the architecture of sleep also changed:percentage of stage 1 increased( (27.7 ± 16.38) % ),percentage of REM sleep increased( (22. 8 ± 6. 1 ) % ) , percentage of stage 2 decreased ((40.2±11.3)%), percentage of slow wave sleep decreased ((11.8 ±9. 32)%); REM sleep significantly changed; decreased REM latency((79. 27 ±30. 44) minutes) , increased REM activity((129. 0 ±53. 12) u) .increased intensity of REM((36.7 ±14.0)u/min), increased REM density((159.2 ±57.2)u/min) were observed in depressed patients. There was no obvious correlation between the variance of REM and severity degree of depression. Conclusion There are a series of changes in sleep progress, architecture and REM sleep of depression and the change of REM sleep can be specified to diagnose depression. However,there is no causality between REM variance and severity of depression.     

The Improbable Association between the Herbicide 2,4-D and Polyneuropathy

Isolated case reports have circumstantially linked the use of the herbicide 2.4-dichlorophen-oxyacetic acid (2,4-D) to polyneuropathy. However, a critical review of the literature reveals numerous reasons for doubting a relationship of 2.4-D to polyneuropathy: (1) too few cases given the wide use of the chemical: (2) no valid toxicologic or epidemiologic evidence; (3) the diversity of antecedent illness; (4) an unlikely time sequence of antecedent illness to exposure (pharmacokinetics); (5) the lack of polyneuropathy in medical patients given repetitive doses of 2.4-D; (6) the lack of polyneuropathy in heavily exposed military personnel involved in operation Ranch Hand; (7) the biological properties of 2.4-D which minimize penetration of 2.4-D into the nervous system under normal exposure conditions; and (8) the lack of polyneuropathy in a variety of experimental animal species given 2,4-D by several routes of exposure and at dose levels and durations of exposure many times greater than human applicator exposure. Thus, the weight of evidence indicates that 2.4-D is an unlikely cause of polyneuropathy. 1990 Academic Press. Inc.     

Molecular differentiation of ischemic and valvular heart disease by liquid chromatography/fourier transform ion cyclotron resonance mass spectrometry

Proteomic patterns of myocardial tissue in different etiologies of heart failure were investigated using a direct analytical approach with High Performance Liquid Chromatography (HPLC)/Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS). Right atrial appendages from 20 patients, 10 with hemodynamically significant isolated aortic valve disease and 10 with symptomatic coronary artery disease were collected during elective cardiac surgery. After preparation of tissue samples and tryptic digestion of proteins, the peptide mixture was HPLC-separated and on-line analyzed by electrospray FT-ICR MS. Data obtained from HPLC / FT-ICR MS runs were compared for classification. To extract the classification features, the selection of best individual features was applied and the "nearest mean classifier" was used for the classification of test samples and the sample projection onto classification patterns. The pattern distribution characteristics of aortic and coronary diseases were clearly different. No interference between samples of both disease categories was registered, even if the distribution of unsupervised classified test samples were closer. Samples representing aortic valve disease showed a closer accumulation pattern of spots compared to the samples representing coronary disease, which indicated a more specific protein classification. Through selective identification of specific peptides and protein patterns with FTMS, valvular and coronary heart disease is for the first time clearly distinguished at molecular level. The described methodology could also be feasible in search for specific biomarkers in plasma or serum for diagnostic purposes.     

急性主动脉夹层临床与影像学诊断研究

【目的】探讨急性主动脉夹层 (AD)临床特点和诊断。【方法】回顾性分析深圳市 5家医院 1995～ 2 0 0 1年收治的5 0例AD患者的临床资料。【结果】长期高血压是AD的主要原因 ,4 5岁以下发病占 18% ;76 %患者表现有剧烈的胸前疼痛 ,亦有隐痛、无痛和剧痛后缓解 ;双上肢和上下肢血压异常差异占 12 % ;主动脉瓣舒张期杂音占 8% ;以并发症急性主动脉分支急性闭塞造成器官缺血坏死或压迫症状为首发症状出现的有急性心肌梗塞 (AMI)、单瘫、截瘫、急性左心衰等 ,亦有少见的气胸和中等量以上心包积液 ;影像检查电子计算机断层 (CT)、磁共振显像 (MRI)、经胸超声心动图 (TTE)有很高的敏感性 ,AD的分型、定位MRI较TTE优 ,而TTE在确定有无主动脉瓣损害、心包积液及观察内膜片活动等方面占优。【结论】AD的临床表现多样、复杂 ,临床医师必须对AD的临床表现及发病机制有清楚认识 ,及时选择或联合应用CT、MRI、TTE等辅助检查是AD确诊的重要手段     

3.0T磁共振图像计算机特征鉴别诊断前列腺良恶性病变的价值

目的 探讨3.0T MRI图像计算机特征鉴别诊断前列腺良恶性病变的价值。方法 收集接受3.0T磁共振检查的前列腺患者330例,进行T1加权成像（T2WI）、T2加权成像（T2WI）、多b值的弥散成像（DWI）与动态增强检查。所有病例均经活检或手术病理证实,其中前列腺癌患者198例,前列腺增生患者132例。选取病变最大层面图像,手动勾画病灶感兴趣区（ROI）,进行方向梯度直方图（HOG）特征、局部二值模式（LBP特征）、哈尔特征（Haar特征）提取。通过支持向量机（SVM）分类器进行训练并得到对应的分类模型,通过不同的分类模型对比选取最具有鉴别前列腺良恶性病变价值的图像特征参数,并采用图像特征分类分析统计方法对所选择的图像特征参数进行评估。鉴别诊断结果以绘制受试者工作特征曲线（ROC曲线）表示,并通过计算曲线下面积（AUC）、灵敏度、特异度、准确率来进行效果评估。结果 磁共振T2WI、长b值DWI、ADC图中,在Haar图像特征条件下,ADC图的AUC值最大（0.85）;在HOG图像特征条件下,DWI序列的AUC值最大（0.78）;在LBP图像特征条件下,ADC图的AUC值最大（0.87）;在采用三种特征融合[HOG+LBP+Haar（HLH）]的图像特征条件下,DWI序列的AUC值较大（0.89）,大于ADC图及T2WI序列的AUC值（0.88、0.84）。图像特征分类分析方法中,LBP图像特征区分两种病变的AUC值较大（0.87）,大于Haar图像特征（0.85）和HOG图像特征（0.78）,HLH融合特征方法的AUC值（0.89）大于其中任一特征,具有最优的鉴别诊断结果。结论 3.0T MRI的T2WI、DWI/ADC图像计算机特征分析有助于鉴别诊断前列腺良恶性病变,长b值的DWI和ADC图对鉴别诊断前列腺良恶性结节占有重要地位,而HLH融合的计算机图像特征分析能为前列腺良恶性病变鉴别诊断提供可靠依据。     

50例急性主动脉夹层患者的临床与影像探讨

目的：分析急性主动脉夹层（AD）的临床特点、诊断以及预后。方法：选择急诊科入院的50例AD病例，对临床表现、确诊方法及预后等进行回顾性分析。结果：引起AD的主要原因主高血压（58．0％），磁共振成像（MPI）的确诊率最高（93．8％），超声心动图（UCG）的确诊率（86．5％）与CT的确诊率（84．6％）相近。结论：AD的临床表现复杂，病情凶险，对怀疑为AD的患者给予UCG、CT、MRI等检查，早日确诊。对AD患者应加强随访，用UCG随访AD患者，有利于早期发现潜在的危及生命的并发症。     

CT与MRI在主动脉夹层诊断中的临床应用价值

目的观察CT与MRI在主动脉夹层(AD)诊断中的临床应用价值。方法对62例AD患者的临床资料进行回顾性分析,根据术前检查方式分为CT组(n=32)和MRI组(n=30)。比较CT与MRI在AD诊断分型以及检出真假腔、撕脱内膜片、内膜破口等的应用价值。结果CT与MRI对AD患者DeBakey分型、真假腔、撕脱内膜片、内膜破口诊断与手术结果符合率均较高,差异无统计学意义(P>0.05)。CT组对主动脉分支受累、主动脉钙化检出率大于MRI组,对附壁血栓检出率小于MRI组(P<0.05)。结论CT与MRI在AD分型、真假腔、撕脱内膜片、内膜破口诊断中均具有良好价值,CT在主动脉分支受累、主动脉钙化检出方面优于MRI,MRI在附壁血栓检出方面优于CT,两种方法各有优劣,临床应根据患者情况适当选择。     

Multi-factor combined biomarker screening strategy to rapidly diagnose Alzheimer's disease and evaluate drug effect based on a rat model

Alzheimer's disease (AD) represents the main form of dementia; however, valid diagnosis and treatment measures are lacking. The discovery of valuable biomarkers through omics technologies can help solve this problem. For this reason, metabolomic analysis using ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS) was carried out on plasma, hippocampus, and cortex samples of an AD rat model. Based on the metabolomic data, we report a multi-factor combined biomarker screening strategy to rapidly and accurately identify potential biomarkers. Compared with the usual procedure, our strategy can identify fewer biomarkers with higher diagnostic specificity and sensitivity. In addition to diagnosis, the potential biomarkers identified using our strategy were also beneficial for drug evaluation. Multi-factor combined biomarker screening strategy was used to identify differential metabolites from a rat model of amyloid beta peptide 1–40 (Aβ₁₋₄₀) plus ibotenic acid-induced AD (compared with the controls) for the first time; lysophosphatidylcholine (LysoPC) and intermediates of sphingolipid metabolism were screened as potential biomarkers. Subsequently, the effects of donepezil and pine nut were successfully reflected by regulating the levels of the abovementioned biomarkers and metabolic profile distribution in partial least squares-discriminant analysis (PLS-DA). This novel biomarker screening strategy can be used to analyze other metabolomic data to simultaneously enable disease diagnosis and drug evaluation.     

膝关节外伤的低场磁共振检查的临床价值

目的探讨膝关节外伤的低场磁共振成像(MRI)表现,以评价膝关节外伤的MRI检查的临床价值。方法回顾性分析我院2003年8月～2009年5月经关节镜、手术证实的63例膝关节外伤患者的MRI资料。结果经MRI证实,63例膝关节外伤患者中半月板损伤38例,与关节镜及手术对比,MRI的诊断准确符合率为94.7%(36/38),并根据Mink和Fischer提出的半月板损伤的MRI信号分级不同,MRI的表现不同。韧带损伤45例,与关节镜及手术对比,MRI的诊断准确符合率为91.1%(41/45)。韧带损伤MRI表现主要为韧带纤维部分纵行撕裂,韧带局部不同程度增生,边缘模糊,出现不同程度纵形高信号。结论 MRI可以清晰显示膝关节损伤部位及程度,是膝关节外伤后较理想的一种非创伤性检查方法。     

Earlier detection of Alzheimer disease using N-fold cross validation approach

According to the recent study, world-wide 40 million patients are affected by Alzheimer disease (AD) because it is one of the dangerous neurodegenerative disorders. This AD disease has less symptoms such as short term memory loss, mood swings, problem with language understanding and behavioral issues. Due to these low symptoms, AD disease is difficult to recognize in the early stage. So, the automated computer aided system need to be developed for recognizing the AD disease for minimizing the mortality rate. Initially, brain MRI image is collected from patients which are processed by applying different processing steps such as noise removal, segmentation, feature extraction, feature selection and classification. The captured MRI image has noise that is eliminated by applying the Lucy–Richardson approach which examines the each pixel in the image and removes the Gaussian noise which also eliminates the blur image. After eliminating the noise pixel from the image, affected region is segmented by Prolong adaptive exclusive analytical Atlas approach. From the segmented region, different GLCM statistical features are extracted and optimal features subset is selected by applying the hybrid wrapper filtering approach. This selected features are analyzed by N-fold cross validation approach which recognizes the AD related features successfully. Then the efficiency of the system is evaluated with the help of MATLAB based experimental results, in which Alzheimer’s Disease Neuroimaging Initiative (ADNI) dataset images are utilized for examining the efficiency in terms of sensitivity, specificity, ROC curve and accuracy.     

MRI诊断血管性痴呆和阿尔茨海默病的价值观察

目的对比分析血管性痴呆(VD)与阿尔茨海默病(AD)磁共振成像(MRI)影像结果。方法回顾性分析2017年4月~2019年4月本院收治的59例VD、42例AD患者临床资料,两组均接受MRI检查,比较两组MRI海马高度、颞角宽度、外侧裂宽度,脑白质疏松(LA)严重度,额叶、颞叶、顶叶、枕叶体积以及各脑叶脑血流参数。结果VD组MRI以脑内梗死灶为主要特征,AD组以海马萎缩为主要特征;VD组海马高度显著高于AD组(P<0.05),颞角宽度、外侧裂宽度均显著低于AD组(P<0.05),LA严重程度大于AD组(P<0.05),额叶、颞叶体积占比显著低于AD组(P<0.05),额叶、颞叶脑血流参数均显著低于AD组(P<0.05)。结论VD与AD患者MRI图像存在较大差异,MRI对VD与AD有较高的鉴别诊断价值。     

多模态MRI评估多灶脑胶质瘤影像学特点及播散形态分布的应用研究

目的分析多模态MRI评估多灶脑胶质瘤影像学特点及播散形态分布。方法选取医院2017年1月至2019年10月收治的多灶脑胶质瘤患者80例患者,采用多模态MRI诊断,比较多模态MRI诊断多灶脑胶质瘤分级、类型、播散形态分布与病理结果符合情况。结果多模态MRI诊断多灶脑胶质瘤低级别、高级别诊断符合率,分类诊断符合率,形态诊断符合率,同侧播散形态符合率均高于误诊率(P<0.05)结论多模态MRI在诊断多灶脑胶质瘤影像学特点、播散形态分布、分类及级别上有价值。