运脾中药对小儿厌食症大鼠模型β—内啡肽的调节作用

目的 观察运脾中药对小儿厌食症大鼠模型中枢和外周β－内啡肽的调节作用。方法 用临床有效运脾中药儿宝颗粒灌胃３周,然后用放兔法测定大鼠下丘脑、垂体、胃窦部及外周血中β－内啡肽的含量。结果 模型动物外周β－内啡肽含量明显减少（Ｐ〈０．０５）,中枢含量无显著变化。大小剂量治疗组血浆β－内啡肽含量恢复正常,大剂量组下丘脑、垂体和胃窦部β－内啡肽含量均显著增加（Ｐ〈０．０１）。结论 运脾中药能促进厌食大鼠中枢和外周β－内啡肽的合成分泌,这一效应可能是运脾中药增加模型大鼠摄食的作用机理之一。     

儿宝颗粒对小儿厌食症动物模型胃窦和血浆β-内啡肽的调节作用

采用病因模拟法建立小儿厌食症动物模型,放免测定该模型血浆和胃窦部β－内啡肽含量,探讨运脾复方儿宝颗粒治疗小儿厌食症的机制。结果表明;模型动物血浆和胃窦部β－内啡肽的浓度明显降低,运脾复方能显著增加胃窦部和血浆中β－内啡肽的浓度。提示β－内啡肽在小儿厌食症发病中起一定作用,运脾复方可通过促进外周β－内啡肽的分泌,达到改善食欲的治疗效果。     

儿宝颗粒对小儿厌食症动物模型胃窦和血浆β—内啡肽？…

采用病因模拟法建立小儿厌食症动物模型,放免测定该模型血浆和胃窦部β－内啡肽含量,探讨运手复方儿宝颗粒治疗小儿厌食症的机制。结果表明：模型动物血浆和胃窦部β－内啡肽的浓度明显降低,运脾复方能显著啬胃部和血浆中β－内啡肽的浓度。提示β－内啡肽在小儿厌食症发病中起一定作用,运脾复方可通过促进外周β－内啡肽的分泌,达到改善食欲的治疗效果。     

运脾复方对小儿厌食症动物模型下丘脑β-内啡肽的影响

目的 研究运脾复方宝颗粒对小儿厌食症动物模型下丘脑β－内啡肽的影响,探讨运脾复方治疗小儿厌食症的机制。方法 用病因模拟法建立幼龄大鼠厌食模型,用放免检测和免疫组化ＡＢＣ法分析模型动物下丘脑β－内啡肽的含量。结果 模型动物下丘脑β－内啡肽合成减少,分泌活动相对增强;运脾复方能促进下丘脑β－内啡肽的合成和分泌。结论 β－内啡肽在小儿厌食症发病中起一定作用,运脾复方可通过增加下丘脑β－内啡肽的合成和分泌     

儿宝颗粒对小儿厌食症动物模型下丘脑β—内啡肽的影响

目的：研究运脾复方儿宝颗粒对小儿厌食症大鼠模型下丘脑β－内啡肽的影响。方法：模拟小儿厌食症病因建立动物模型。用免疫组化方法观察动物下丘脑β－内啡肽的分布结合图像分析技术测定相对含量。结果：模型动物下丘脑β－内啡肽含量降低（Ｐ〈０．０５）。治疗组β－内啡肽含量增高（Ｐ〈０．０５）。结论：运脾复方能促进模型动物下丘脑β－内啡肽合成,可能是治疗小儿厌食症的重要作用机理之一。     

儿宝颗粒剂对幼鼠厌食动物模型下丘脑、垂体、胃窦及血浆中β-内啡肽含量的影响

模拟小儿厌食症主要病因制作幼龄大鼠动物模型 ,用临床有效运脾复方儿宝颗粒剂治疗 ,测定大鼠下丘脑、垂体、胃窦及血浆中 β -内啡肽含量 ,观察运脾复方对该模型中枢和外周 β -内啡肽的调节作用。结果显示模型动物与正常对照动物相比 ,血浆和胃窦 β -内啡肽含量明显减少 (P<0 .0 5) ,而下丘脑、垂体含量变化不大 ;大小剂量治疗组血浆 β -内啡肽恢复正常 ,大剂量组下丘脑、垂体和胃窦 β -内啡肽含量增加 ,显著高于正常组 (P <0 .0 0 1)。     

儿宝颗粒对幼龄厌食大鼠下丘脑中β-内啡肽含量的影响

目的：研究β－内啡肽在幼龄厌食大鼠治疗前后下丘脑的含量变化及其与小儿厌食症的发病关系。方法：模拟小儿厌食症病因建立幼龄厌食大鼠模型,并用运脾方药治疗,用免疫组化方法观察正常组、模型组、治疗组下丘脑β－内啡肽的含量变化,结合图像分析技术测定其结果。结果：模型动物β－内啡肽低于对照组（Ｐ〈０．０５）,儿宝颗粒治疗组下丘脑中β－内啡肽的含量较模型组显著增高（Ｐ〈０．０１）。结论：下丘脑β－内啡肽的含量降     

慢性酒精刺激对大鼠下丘脑及伏核内β-内啡肽含量的影响

目的：观察在长期代浓度酒精处理后及撤除酒精给药的过程中,大鼠下丘脑及伏核中β－内啡肽含量的变,探讨酒精对β－内啡肽系统的影响及其在酒精依赖过程中的作用,方法：以6%（v/v)的酒精作为大鼠饮水的唯一来源,分别取大鼠在饮酒和撤除酒精不同时程的下丘脑及伏核标本,用放免法测定每克组织中β－内啡肽的含量,同时用顶空气相色谱法测定大鼠在饮酒过程中不同时段血中的酒精浓度,结果：下丘脑及伏核的β－内啡肽含量在生长期（4周）饮用酒精后无明显变化,在撤除酒精后的2－4天明显升高,1周后恢复正常,动物血中酒精浓度在摄入酒精的不同时段无明显差异,结论：酒精影响下丘脑及伏核中β－内肽的含量是低浓度反复多次刺激的结果,撤药的伏核内β－内啡肽的高含量是动物产生依赖的重要原因。     

开胃消食糖浆对幼龄厌食大鼠模型CCK-8和β-Ep的影响

目的研究开胃消食糖浆对幼龄厌食大鼠模型摄食及八肽胆囊收缩素(CCK-8)和β内啡肽(β-Ep)含量的影响。方法用特制饲料喂养大鼠,制作幼龄厌食大鼠模型,采用开胃消食糖浆治疗,观察各组大鼠摄食量的变化,并运用放免检测技术测定各组动物下丘脑及外周血中CCK-8和-βEp的含量。结果模型组摄食量明显下降,其下丘脑和血浆CCK-8浓度显著高于正常组(P<0.05),下丘脑β-Ep浓度与正常组差异无统计学意义(P>0.05),而胃窦黏膜和血浆-βEp浓度显著低于正常组(P<0.01,P<0.05)。开胃消食糖浆治疗后,动物摄食量在第1周末明显增加(P<0.05),其下丘脑和血浆CCK-8浓度较模型组降低(P<0.05),下丘脑、胃窦黏膜和血浆-βEp浓度也较模型组显著增加(P<0.05)。结论幼龄厌食大鼠模型中枢和外周CCK-8浓度增高,外周-βEp的浓度降低,对中枢-βEp的浓度无明显的影响,开胃消食糖浆能明显促进中枢和外周血-βEp分泌,抑制中枢和外周血CCK-8的分泌,从而增加食量。     

二甲肼诱发大鼠大肠癌时下丘脑、垂体及脑桥ir－β－EP含量的变化

应用放射免疫法测定了二甲肼（ＤＭＨ）诱发大鼠大肠癌过程中下丘脑、垂体及脑桥组织中β－内啡肽样免疫活性物质（ｉｒ－β－Ｅｎ含量变化。结果显示：肿瘤组动物下丘脑ｉｒ－β－ＥＰ含量显著高于对照组；增生组下丘脑、垂体ｉｒ－β－ＥＰ含量亦显著升高；各组脑桥组织中ｉｒ－β－ＥＰ含量无明显变化。提示中枢神经系统中下丘脑－垂体轴分泌的β－内啡肽（β－ＥＰ）可能参与了大肠癌的发生、发展过程。     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.     

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