空肠弯曲菌外膜蛋白SDS-PAGE应用于流行病学调查的研究

用SDS-PAGE分析不同来源41株空肠弯曲菌外膜蛋白特征,根据外膜蛋白图谱蛋白带带到组合情况及主带迁移率可将其各分为7个和9个类型.对人源和鸡等动物源菌株外膜蛋白类型的比较,提示鸡等动物是儿童空肠弯曲菌感染的传染源。对腹泻患儿和健康携带者菌株外膜蛋白类型的比较,提示空肠弯曲菌的致病性可能与其外膜蛋白特征有关.用此方法,作者分析了一起幼儿园空肠弯曲菌感染的流行病学关系,结果表明该感染呈散发多源性;人-人传播对空肠弯曲菌感染具有意义.     

广州地区空肠弯曲菌抗原性初步分析

为了解广州地区鸡来源和腹泻病人来源空腹弯曲菌之间的关系,以及其抗原性情况,我们制备了十株空肠弯曲菌免疫血清,用间接血凝试验法对16株空肠弯曲菌可溶性抗原进行了交叉凝集试验。本结果表明,不同来源菌株有明显特异性,菌株间交叉反应较弱,广州地区人或鸡来源菌株的抗原性与 Lior's 2074株有低度交叉(1:40～1:80),人来源菌株免疫血清与鸡来源菌株间亦有低度交叉。     

深圳市市售家禽空肠弯曲菌带菌状况调查

目的了解深圳市当前三鸟市场鸡、鸭、鹅的空肠弯曲菌带菌情况,为进一步开展食品安全性研究,制定防控食源性空腑弯曲菌病的措施提供科学依据。方法从三鸟批发市场采取三鸟肛拭标本,依据GB/T4789．19—2003方法进行细菌检验。结果50份鸡肛拭子共检出空肠弯曲菌20株,阳性率为40％;20份鸭肛拭子共检出空肠弯曲菌2株,阳性率为10％;30份鹅肛拭子共检出空肠弯曲菌6株,阳性率为20％。检出的空肠弯曲菌均为空肠亚种。结论深圳市活禽空腑弯曲菌感染或带菌率较高,如果在销售或加工过程中卫生质量不良,会对消费者的健康构成潜在威胁。     

空肠/结肠穹曲菌对HeLa细胞粘附作用的研究

利用光镜、电镜和免疫荧光染色等技术,研究了13株空肠/结肠弯曲菌对HeLa细胞的粘附和侵袭作用。结果表明;13株细菌均能粘附到HeLa细胞表面,其中8株从急性腹泻病人粪便中分离出的细菌粘附作用比4 株动物株强。电镜研究证实:细菌能粘附并穿入到HeLa细胞内,引起细胞病变。细菌的鞭毛在粘附中起一定作用。     

20株空肠弯曲菌对8种抗菌药物的敏感性

空肠弯曲菌(Campylobacter fetus subspecies jejuni)作为常见的肠道致病菌,尤其是小儿肠炎的常见病原菌,愈来愈引起重视。为了解空肠弯曲菌对抗菌药物的敏感性,以指导临床对该菌所致肠炎的治疗,我们于1982年对20株空肠弯曲菌分别进行了8种药物的敏感试验,兹将结果报告如下: 试验用菌株系1982年6月～7月由我院的门诊腹泻患儿粪便分离所得。患儿年龄最小67天,最大13岁。所有菌株均经生化试验鉴定为空肠弯曲菌。 试验用药物及方法:试验用药物见附表:     

广州地区不同人群的空肠弯曲菌抗体及其临床意义

为了解广州地区空肠弯曲菌感染情况和健康人群抗体滴度的临界值,我们于1984年5月—10月用间接血凝试验法检测276份各类人群血清。实验结果:健康人群抗体滴度的临界值为1:42.7,若病人血清≥1:80有临床意义。本次检查活鸡中空肠弯曲菌检出率为69.4%,熟鸡为1%。检查广州不同人群鸡来源空肠弯曲菌抗体检出率较高,由12.2—45.6%,尤其腹泻患儿和抗 O 血清中空肠弯曲菌抗体检出率分别为45.6%和44%,与健康人群比较有非常显著性差异(P<0.001),说明鸡来源空肠弯曲菌感染普遍,可能与广州人爱吃白切鸡(未熟透)有关。在抗 O 血清和肝功试验血清检查中,空肠弯曲菌抗体滴度>1:80者分别为14%和7.5%,因此要注意误诊及漏诊问题。本次从腹泻患儿粪便检出空肠弯曲菌者为6/57占10.5%,而腹泻患儿检查空肠弯曲菌抗体滴度>1:80者为5/46占10.8%,说明抗体检查与细菌分离结果一致。     

深圳市腹泻患者、禽源和牛源空肠弯曲菌的脉冲场凝胶电泳分析

目的 对深圳市禽源、牛源空肠弯曲菌进行脉冲场凝胶电泳,与相同时期和地区腹泻患者分离的空肠弯曲菌比较,寻找空肠弯曲菌传播的证据。方法 对深圳市2016年3—11月分离的不同来源空肠弯曲菌进行复苏、鉴定和脉冲场凝胶电泳,应用BioNumerics软件进行聚类分析。结果 共收集到空肠弯曲菌126株,其中腹泻患者来源22株、禽源44株、牛源60株。除3株不能分型外,其他123株菌成功获得PFGE图谱。不同来源空肠弯曲菌呈现不同的基因特征：人源菌株PFGE带型高度多态化,未发现集中型别;P1是禽源空肠弯曲菌的主要型别;牛源菌株呈现多型别分布,主要型别有C1~C6。综合分析发现,有3个型别（T1、T2和T3）同时包含患者和禽牛来源的菌株。T1型别有12株菌,分别来自患者（3株）、禽类（6株）和牛（3株）,T2型别包含5株菌,来自患者（1株）、禽类（2株）和牛（2株）;T3型别包含7株菌,仅来自患者（1株）和牛（6株）。结论 本研究发现3个空肠弯曲菌型别（T1,T2和T3）同时包含腹泻患者和禽牛来源菌株,为空肠弯曲菌的溯源提供证据。T1可能是深圳市空肠弯曲菌的主要流行型别,在腹泻患者、禽源、牛源菌株中均有分布。     

榆林市2010年食品中食源性致病菌检测结果分析

目的了解榆林市食品中食源性致病菌污染情况。方法随机采集各类食品165份,分别进行沙门氏菌、大肠杆菌O157、单核细胞增生李斯特菌、创伤弧菌、金黄色葡萄球菌、副溶血性弧菌、空肠弯曲菌7项检测,依据中国疾病预防控制中心营养与食品安全所提供的《2010年食源性致病菌监测工作手册》(内部资料)和国家标准《食品卫生微生物学检验》进行检测。结果此次共检出食源性致病菌33株,总检出率20.00%,其中沙门氏菌15株、单核细胞增生李斯特菌9株、金黄色葡萄球菌7株、副溶血性弧菌2株,未检出大肠杆菌O157、空肠弯曲菌、创伤弧菌。结论榆林市居民主要消费的食品中肉类及豆制品食源性致病菌受污染较严重,应加大食品加工行业卫生监控力度,有效预防食源性疾病的暴发流行。     

腹泻患者空肠弯曲菌检出结果和药敏试验的分析

胎儿弯曲菌空肠亚种(Campylobacter fetus subsp jejuni,简称空肠弯曲菌),目前已被认为是人类肠道感染的一种常见和重要的病原体,在急性腹泻患者中与其他检出的肠道致病菌相比占首位。为了解空肠弯曲菌对不同年龄、性别腹泻患者的致病力以及对抗生素的敏感情况,现将398例急性腹泻患者新鲜粪便分离出的41株空肠弯曲菌分析如下。     

亲水气单胞菌肠炎的临床与实验研究——附26例分析(摘要)

本文报告1983年3月至1984年2月26例亲水气单胞菌肠炎的临床与实验研究结果。800例成人急性腹泻患者粪便细菌培养结果:痢疾杆菌170株、空肠弯曲菌60株、致病性大肠杆菌28株、亲水气单胞菌26株、副溶血弧菌16株、沙门菌12株;检出率分别为21.25％、7.5％、3.5％、3.25％、2％和1.5％。从中看出急性感染性腹泻的致病菌种类繁多,且亲水气单胞菌亦可成为急性感染性腹泻的一种致病菌。添加氨苄青霉素的培养基为分离本菌的较理想培养基。本菌均经形态学、生长特性和生化反应等进行鉴定,且作肠毒素试验。药敏检测结果显示本菌对氨基甙类抗生素等呈敏感,与国外报告相     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.