吡喹酮治疗脑囊虫病患者血清中Th1/Th2细胞因子的变化及临床意义

目的探讨脑囊虫病患者经吡喹酮治疗后,机体免疫应答的状态和对病程转归影响。方法35例脑囊虫病患者经吡喹酮治疗6个月后,对血清中IL-2、INF-γ、IL-5、IL-10、TNF-α、IL-12进行检测。结果治疗6个月后,Th1型细胞因子IL-2、IFN-γ、IL-12明显升高(P<0.01),Th2型细胞因子IL-5和IL-10在患者中检出率明显下降(P<0.01),TNF-α水平较治疗前显著下降(P<0.05)。治疗组对药物反应良好的患者,IL-2、IFN-γ、IL-12明显高于对药物反应不良者。结论脑囊虫病患者治疗后Th2型细胞因子反应减弱,而Th1型细胞因子反应增强,机体的保护性免疫应答已经开始恢复。     

系膜增生性肾小球肾炎患者血清Th1/Th2细胞因子表达的变化

目的 观察系膜增生性肾小球肾炎患者血清Th1和Th2类细胞因子(INF-γ、IL-4、IL-10)表达变化.方法 采用酶联免疫吸附实验(ELISA)测定10例正常人和25例慢性肾小球疾病患者(轻中度系膜增生性非IgA肾小球肾炎)外周静脉血血清INF-γ、IL-4、IL-10细胞因子水平.结果 系膜增生性肾小球肾炎患者血清INF-γ分泌水平较正常人明显增多(P<0.01),而IL-10分泌水平较正常人明显减少(P<0.05),IL-4分泌水平则与正常人无显著差异(P>0.05),系膜增生性肾小球肾炎患者INF-γ/IL-4、INF-γ/IL-10值均显著高于正常人(P<0.05).结论 系膜增生性肾小球肾炎患者在外周血细胞因子水平存在Th1/Th2免疫失衡,可能参与了系膜增生性肾小球肾炎的免疫发病机制.     

脑梗塞患者T辅助细胞的免疫应答方式

目的探讨脑梗塞患者外周血T辅助淋巴细胞亚群功能失衡及意义。方法取观察组(脑梗塞组)及正常对照组(健康者)静脉血,分离单个核细胞并于37℃、5%CO2培养箱中培养72h,分离上清,ELISA法测定上清液中INF-γ/IL10含量,计算INF-γ/IL10的比值。结果观察组INF-γ/IL10的比值明显高于正常对照组。结论脑梗塞患者存在T细胞亚群功能失衡,主要表现为Th1细胞功能亢进,其可能是脑梗塞患者免疫异常所致。     

保胎灵Ⅰ号治疗反复自然流产对母体免疫平衡及内分泌功能的影响

目的:探讨中药治疗对反复自然流产(RSA)患者异常母胎免疫及内分泌的影响.方法:用自制保胎方剂保胎灵Ⅰ号治疗RSA患者90例,采用酶联免疫吸附法检测90例RSA患者(A组)、30例正常非妊娠妇女(B组)和30例正常妊娠妇女(C组)中药治疗前后由清Th1型细胞因子和Th2型细胞因予水平以及妊娠后血泌乳素PRL、孕酮P、雌激素E的动态变化.结果:中药冶疗RSA后,Th1型细胞因子IFN-γ、TNF-α浓度降低,而IL4、IL10明显升高,差异均有显著性意义,母胎界面Th1/Th2型细胞因子平衡恢复正常.治疗后妊娠68例,保胎成功62例,占91.2%,保胎失败6例,占8.8%.保胎成功组外周血PRL、P水平随孕期增加显著升高,而保胎失败者无明显变化.结论:中药可能通过调节母胎内分泌和免疫网络的棚互应答达到保胎治疗作用.     

2型和3型辅助性T(Th)细胞对滋养层细胞的免疫是体外受精-胚胎移植成功的标志

目的:本研究旨在探讨体外受精-胚胎移植(IVF-ET)中,由外周血单核细胞(PBM C)分泌的1、2、3型Th细胞因子是否分别与治疗成败或成功相关。设计:队列研究。机构:医疗学术中心。患者:101例行IVF-ET的患者为研究组,19例有生育力的正常妇女为对照组。干预:分别抽取取卵前研究组患者及19例未孕状态对照人群的外周血。从滋养层细胞系或精子细胞膜中提取蛋白,将PBM C分别培养在含或不含上述提取蛋白的培养基中。主要观察指标:用ELISA法检测培养基中PBM C分泌的1型Th细胞因子如肿瘤坏死因子α(TNF-α)及干扰素γ(IFN-γ),2型Th细胞因子…     

滴虫性阴道炎患者阴道局部Th1/Th2细胞因子与pH值、菌群密集度的相关性分析

目的：滴虫性阴道炎(TV)患者阴道局部Th1/Th2细胞因子与pH值、菌群密集度的相关性分析。方法：选取2019年6月—2021年6月我院收治的104例TV患者，根据阴道分泌物Nugent评分分为过渡态TV组(Nugent评分4～6分，33例)、中度TV组(Nugent评分7～8分，54例)、重度TV组(Nugent评分9～10分，17例),另以1∶3比例选取同期312例健康体检者为对照组，均采用酶联免疫吸附法检测Th1型细胞因子[白介素-2(IL-2)、干扰素-γ(INF-γ)]、Th2型细胞因子[白介素-4(IL-4)、白介素-10(IL-10)],计算Th1/Th2细胞因子比值，检测阴道局部pH值及菌群密集度，以Spearman相关性分析Th1/Th2细胞因子与pH值、菌群密集度的关系。结果：过渡态TV组、中度TV组、重度TV组IL-2/IL-4、IFN-γ/IL-4、IL-2/IL-10、IFN-γ/IL-10水平均低于对照组(P<0.05);中度TV组IL-2/IL-10、IFN-γ/IL-10低于过渡态TV组(P<0.05),重度TV组IFN-γ/IL-4、IL...     

急性髓性白血病患者斯奇康治疗前后Th1/Th2细胞因子变化及临床意义

目的:观察急性髓性白血病(AML)患者Th1/Th2细胞因子变化及使用斯奇康后的变化。方法:用酶联免疫吸附试验(ELISA)测定AML患者血中Th1细胞因子IL-2和INF-γ及Th2细胞因子IL-4和IL-10水平,并与健康对照人群的比较;AML患者在常规治疗的同时,用斯奇康肌肉注射0.3mg/次,2次/周,共18次,同法测定上述细胞因子水平,与治疗前比较。结果:AML患者与健康人群比较,Th1细胞因子IL-2和INF-γ均显著降低(P<0.01),而Th2细胞因子IL-4和IL-10显著增加(P<0.01);与斯奇康治疗前比较,治疗后Th1细胞因子IL-2和INF-γ均增加(P<0.05),而Th2细胞因子IL-4显著降低(P<0.01),IL-10亦有降低,但与治疗前比较差异无显著性(P=0.076)。结论:AML患者存在Th1功能低下而Th2功能相对亢进的免疫失衡状态,斯奇康有改善AML患者上述免疫失衡的功能。     

苦参胶囊对湿热蕴肤型慢性湿疹患者的血清Th1、Th2细胞因子的水平影响

目的:探讨湿热蕴肤型慢性湿疹患者采用苦参胶囊治疗后的血清Th1、Th2细胞因子水平的变化。方法:选取湿热蕴肤型慢性湿疹患者63例,按随机数字表法分为对照组和研究组,其中对照组32例患者采用盐酸西替利嗪治疗;研究组31例患者则联合苦参胶囊治疗,比较两组患者治疗后的Th1、Th2型细胞因子:干扰素-γ(INF-γ)、白细胞介素2(IL-2)、白细胞介素4(IL-4)的水平变化,同时分析患者的临床治疗有效率。结果:(1)治疗后,研究组患者的Th1细胞因子INF-γ、IL-2水平均明显低于对照组患者;研究组患者Th2细胞因子IL-4明显高于对照组患者(P<0.05)。(2)研究组患者的临床治疗有效率为93.55%,明显高于对照组患者的71.88%(P<0.05)。结论:湿热蕴肤型湿疹患者采用苦参胶囊进行治疗,可调节患者的INF-γ、IL-2、IL-4的水平,恢复Th1、Th2细胞因子平衡,疗效突出。     

Th17细胞与肝脏疾病的研究进展

目前,根据细胞所产生细胞因子及功能的不同,辅助性CD4+T细胞可分为Thl、Th2、Thl7和调节性T细胞(Treg)四个亚群。在以往的研究中发现Th1细胞大多参与机体细胞免疫反应的发生,主要通过分泌肿瘤坏死因子α(TNF-α)、γ干扰素(IFN-γ)、白介素(IL)-2等细胞因子发挥生物效应;而Th2型细胞则主要通过分泌IL-4、IL-10、IL-13等多种细胞因子,促进机体体液免疫反应的发生;Treg细胞通过抑制过度的免疫反应,对自身免疫发挥保护作用,并参与机体的免疫平衡;     

Th1/Th2平衡与胃癌前病变危险因素及病理积分相关性分析

目的探讨Th1/Th2免疫平衡与胃癌前病变危险因素及病理积分的关系。方法采用酶联免疫法(ELISA)测定100例胃癌前病变患者血清Th1(IL-2、INF-γ)、Th2(IL-4、IL-6)含量,采集、检测患者年龄、性别、HP感染、遗传、饮食结构及病理积分,并逐一进行相关性分析。结果 HP感染患者细胞因子INF-γ、INF-γ/IL-4明显降低(P0.05),IL-4明显升高(P0.05),不良饮食结构患者IL-4的表达明显升高,INF-γ/IL-4明显降低(P均0.05)。胃癌前病变胃镜病理积分与INF-γ/IL-4比值存在负相关关系。结论 HP感染、不良的饮食习惯等危险因素能使人体Th1/Th2免疫发生漂移,从而导致异常增生甚至癌变。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…