Survivin FZD10在骨肉瘤中的表达及临床意义

目的探讨骨肉瘤组织中Survivin蛋白和FZD10的表达及其临床意义。方法应用免疫组织化学技术(SP法)检测22例骨肉瘤(实验组)组织及11例骨软骨瘤(对照组)组织中抑凋亡蛋白Survivin及血管内皮生长因子FZD10的表达水平,分析其与骨肉瘤发生的相关性。结果 Survivin蛋白在骨肉瘤、骨软骨瘤组织中的表达为64%(14/22)和0(0/11)(P<0.05);FZD10蛋白在骨肉瘤、骨软骨瘤组织中的表达为82%(18/22)和9%(1/11)(P<0.05);并且蛋白表达和肿瘤恶性程度呈正相关;结论 Survivin蛋白和FZD10蛋白的高表达与骨肉瘤的发生发展密切相关。两者的表达具有相关性可作为诊断骨肉瘤,判断其恶性程度的参考指标。     

PTEN和Gaspase-3在骨肉瘤中的表达

目的探讨抑癌基因PTEN蛋白和Caspase-3在骨肉瘤中的表达及在其发生发展中的可能作用.方法用免疫组织化学方法检测PTEN和Caspase-3在38例骨肉瘤及20例骨软骨瘤中的表达.结果PTEN蛋白在骨肉瘤中,表达阳性率为55.3%.在对照组骨软骨瘤中为90.0%,骨肉瘤与骨软骨瘤相比有显著差异(P＜0.01).Caspase-3在骨肉瘤中表达阳性率为52.6%,在对照组骨软骨瘤中为85.0%,骨肉瘤与骨软骨瘤相比有显著差异(P＜0.05).结论PTEN蛋白和Caspase-3与骨肉瘤的发生有关.     

核因子κB与细胞周期蛋白D1在骨肉瘤中的表达及相关性研究

目的:探讨骨肉瘤细胞中核因子κB(NF-κB)及细胞周期蛋白D1(cyclin D1)的表达及其意义.方法:应用免疫组织化学SABC法,检测46例骨肉瘤、15例骨软骨瘤和5例正常骨组织中NF-κBp65亚单位和cyclin D1表达,并比较NF-κB表达在骨肉瘤组织学分级、分型间的差异及其与cyclin D1表达的关系.结果:NF-κBp65亚单位显著表达于骨肉瘤细胞中(67.4%),而在正常骨组织和骨软骨瘤中未见表达;NF-κBp65表达与骨肉瘤组织学分级无关,在骨内高分化型组表达显著高于普通中心型组(P＜0.05).cyclin D1在骨肉瘤中表达率为73.9%,显著高于骨软骨瘤组(33.3%,P＜0.05);骨肉瘤中NF-κB p65与cyclin D1蛋白表达显著正相关(r=0.463,P＜0.01).结论:NF-κB p65在骨肉瘤细胞中异常活化,并与cyclin D1过表达密切相关,其可能通过上调cyclin D1表达参与骨肉瘤的发生发展.     

XIAP和Caspase-3在骨肉瘤中的表达及其意义

目的研究骨肉瘤中X相关凋亡抑制蛋白(x chromosome linked inhibitor of apoptosis protein,XIAP)和胱天蛋白酶-3(Caspase-3)的表达及其临床意义。方法应用免疫组化方法检测38例骨肉瘤和10例骨软骨瘤中XI-AP和Caspase-3的阳性表达情况。结果10例骨软骨瘤中,Caspase-3表达7例阳性,XIAP表达均阴性。在骨肉瘤组中Caspase-3阳性表达率18.4%;而XIAP阳性表达率68.4%,显著高于骨软骨瘤(P0.05)。XIAP蛋白的表达与患者的年龄、性别、病理分型、临床分期无相关。骨肉瘤组中,Caspase-3阳性表达率为18.4%,其中低度恶性、组织细胞分化中等G1期骨肉瘤表达明显高于高度恶性、组织细胞分化差的G2期(P0.05)。Caspase-3的表达与患者的年龄、性别不相关。骨肉瘤组中Caspase-3和XIAP表达趋势呈负相关性(P0.05)。结论骨肉瘤组中XI-AP呈高表达,Caspase-3呈低表达;骨软骨瘤中Caspase-3呈高表达,XIAP呈低表达;骨肉瘤组中XIAP和Caspase-3表达趋势呈负相关。     

人骨肉瘤组织中cyclinE和ki-67蛋白的表达

目的 :探讨人骨肉瘤组织中cyclinE和ki 6 7蛋白的表达。方法 :采用免疫组化S P法检测 30例骨肉瘤和10例骨软骨瘤组织中cyclinE和ki 6 7蛋白的表达情况。结果 :骨肉瘤组织中cyclinE及ki 6 7蛋白阳性表达率分别为 93.33%和 6 6 .6 7% ,骨软骨瘤中分别为 30 %和 10 % ,2者的阳性表达率在不同组织中差异有统计学意义 (P <0 .0 5 )。cyclinE蛋白表达强度与ki 6 7蛋白表达强度之间呈正相关 (rs=0 .6 4 1,P <0 .0 1)。结论 :骨肉瘤组织中cyclinE和ki 6 7蛋白均为高表达 ,它们与骨肉瘤的发生、发展有相关性。     

Aurora-B在骨肉瘤中的表达及与远处转移的关系

目的 通过对Aurora-B在骨肉瘤中的表达及其与远处转移关系的研究,探讨其是否参与肿瘤转移.方法 收集60例经外科手术切除的骨肉瘤组织,10%甲醛固定,常规石蜡包埋.采用免疫组织化学方法检测Aurora-B在骨肉瘤组织中的表达,另取21例骨软骨瘤作为对照组.同时采用Western blot法检测Aurora-B蛋白在骨肉瘤细胞株U2-OS的表达情况.结果 免疫组织化学法检测显示Aurora-B表达在细胞核.骨肉瘤组织标本中53.3%阳性表达,骨软骨瘤组织中14.3%阳性表达.骨肉瘤组织和骨软骨瘤组织中Aurora-B的表达比较差异有显著性(P＜0.05),但与组织类型无相关性.在14例发生远处转移的骨肉瘤标本组织中11例Aurora-B阳性表达;阳性表达率为78.6%明显高于未发生远处转移的标本组织中Aurora-B阳性表达率45.7%(21/46),而且有丝分裂期的骨肉瘤细胞Aurora-B染色较深.Western blot法检测同样证实Aurora-B在人骨肉瘤细胞株U2-OS中阳性表达.结论 Aurora-B极有可能参与了骨肉瘤的发生、发展和远处转移,有可能成为骨肉瘤新的诊断及分子治疗靶点.     

PTEN在骨肉瘤组织中的表达及意义

目的:研究骨肉瘤组织中抑癌基因PTEN的表达情况,探讨其在骨肉瘤发生、发展中的可能作用.方法:用免疫组化S-P法检测PTEN蛋白在38例骨肉瘤及20例骨软骨瘤石蜡标本中的表达情况.结果:PTEN蛋白在骨肉瘤中表达阳性率为55.3%,在骨软骨瘤中为90.0%,骨肉瘤与骨软骨瘤相比有显著差异(P＜0.01).结论:抑癌基因PTEN蛋白在骨肉瘤组织中表达下调,可能与骨肉瘤的恶性进展有关.     

GRIM-19和STAT3在骨肉瘤中的表达及意义

目的：观察骨肉瘤中GRIM-19与STAT3的表达情况,探讨二者与骨肉瘤临床病理特征的关系及骨肉瘤二者表达的相关性。方法：采用免疫组织化学染色SP法检测35例骨肉瘤和14例骨软骨瘤GRIM-19与STAT3的表达情况。结果：骨肉瘤中STAT3的阳性表达率明显高于骨软骨瘤,GRIM-19的阳性表达率明显低于骨软骨瘤,差异均具有统计学意义(P＜0.01);骨肉瘤中STAT3表达与Enneking分期、肺转移有关,GRIM-19表达与Enneking分期有关(P＜0.05);骨肉瘤中STAT3表达与GRIM-19呈负相关(P＜0.05)。结论：骨肉瘤组织中存在STAT3高表达和GRIM-19低表达共存现象,二者共同作用可能与骨肉瘤的发生、发展密切相关。     

人骨肉瘤中P27、cyclinE和ki-67的表达

目的 :探讨P2 7、cyclinE和ki 67蛋白在骨肉瘤发生、发展中的作用 ,了解其在人骨肉瘤和骨软骨瘤中的表达。方法 :采用免疫组化S P法检测了 3 0例骨肉瘤和 10例骨软骨瘤中P2 7、cyclinE和ki_67蛋白的表达情况。结果 :P2 7蛋白在骨肉瘤和骨软骨瘤中阳性表达率分别为 3 3 3 3 %和 80 0 0 % ,二者差异有显著性 (P<0 0 5 )。P2 7蛋白在骨肉瘤和骨软骨瘤中表达强度的差异有显著性 (P <0 0 5 )。cyclinE蛋白在骨肉瘤和骨软骨瘤中阳性表达率分别为 93 3 3 %和 3 0 0 0 % ,二者相比差异有显著性 (P <0 0 5 )。cyclinE蛋白在骨肉瘤和骨软骨瘤中表达强度的差异有显著性 (P <0 0 5 )。ki 67蛋白在骨肉瘤和骨软骨瘤中阳性表达率分别为66 67%和 10 0 0 % ,二者差异有显著性 (P <0 0 5 )。ki 67蛋白在骨肉瘤和骨软骨瘤中表达强度的差异有显著性 (P <0 0 5 )。P2 7和cyclinE蛋白在骨肉瘤中的表达强度呈显著负相关 (rs=-0 43 2 ,P <0 0 5 )。P2 7蛋白表达强度与ki 67表达强度之间无相关性 (rs=-0 0 3 4,P >0 0 5 )。cyclinE蛋白表达强度与ki_67蛋白表达强度之间呈显著正相关 (rs=0 64l,P <0 0 1)。结论 :P2 7蛋白在骨肉瘤中呈低表达 ,cyclinE和ki 67蛋白在骨肉瘤中均为高表达 ,三者与骨肉瘤发生、发展密     

细胞周期调控因子p27在骨肉瘤中的表达及其临床意义

目的:探讨细胞周期负性调控因子p27在骨肉瘤组织中的表达及其临床意义。方法:应用S-P免疫组织化学方法检测46例骨肉瘤和l6例骨软骨瘤组织中p27的表达,并观察p27表达在骨肉瘤组织学分型、分化程度等临床指标之间的差异。结果:46例骨肉瘤组织中p27的阳性表达率分别为43.5%,而在骨软骨瘤的阳性表达率为81.5%,与骨肉瘤组比较有统计学意义(P<0.01);骨肉瘤中,低分化组织、临床分期为Ⅱb~Ⅲa及肿瘤转移患者p27的阳性率分别低于高分化组织、临床分期为Ⅰb~Ⅱa及肿瘤无转移患者,差异有统计学意义(P<0.05);p27在骨肉瘤中的表达在不同性别、年龄和组织类型间差异无统计学意义(P>0.05)。结论:在骨肉瘤中,p27的表达与临床分期、分化程度及肿瘤的转移有关,提示p27的检测可作为骨肉瘤的发生、发展及不良预后的判断指标之一。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…