茶多酚对人γδT淋巴细胞和结肠癌细胞株SW-480的影响

目的:观察茶多酚对人γδT细胞杀伤肿瘤细胞活性的影响和诱导人结肠癌细胞株(SW-480)凋亡和死亡的作用。方法:在体外用不同浓度的茶多酚诱导人γδT细胞和SW-480细胞株,然后测定人γδT细胞杀瘤活性和对肿瘤细胞的作用。用不同浓度的茶多酚分别诱导SW-480细胞4 h后,弃含有茶多酚的培养液再继续培养24 h,然后测定其死亡和凋亡数,用γδT细胞作对照组。结果:茶多酚浓度350 mg/L,作用γδT细胞4 h后能明显增强γδT细胞的杀伤肿瘤细胞活性,与未诱导组和其他浓度组相比,P<0.01;γδT细胞对经茶多酚处理后的SW-480细胞杀伤活性也明显高于对照组(P<0.01)。各种浓度的茶多酚分别与γδT和SW-480细胞作用4 h,弃诱导液再继续培养24 h后,均能诱导其死亡和凋亡,在茶多酚浓度350 mg/L、诱导时间4 h时最明显;同一浓度的茶多酚诱导SW-480细胞死亡和凋亡率明显高于γδT细胞组。结论:茶多酚在一定浓度时能明显提高γδT细胞对肿瘤的杀伤活性;经茶多酚处理的SW-480细胞更易被γδT细胞杀伤。茶多酚浓度在350 mg/L时能诱导SW-480细胞凋亡而对人γδT细胞无影响。     

体外扩增CIK细胞对结肠癌SW480细胞株杀伤作用的研究

目的：观察细胞因子诱导的杀伤细胞（CIK细胞）体外增值情况,了解其扩增后杀伤结肠癌SW480细胞株最佳比例及最有效的维持时间.方法：分离健康人外周血单个核细胞在体外诱导制备成CIK细胞,计数不同培养时间的CIK细胞,采用流式细胞仪检测其表型特征.倒置显微镜下观察CIK细胞作用与SW480结肠癌细胞的形态学变化,采用HE染色、AO/EO荧光染色和MTT法检测CIK细胞对SW480结肠癌细胞株的杀伤活性.结果：CIK细胞在体外培养14 d细胞增殖倍数为（100.22±8.68）倍,培养21 d细胞增殖倍数为（300.86±10.13）倍,两者比较差异有统计学意义（P＜0.05）;培养28 d细胞增殖倍数为（305.06±5.13）倍,与培养21 d的细胞增殖倍数比较差异无统计学意义（P＞0.05）.培养21 d的CIK细胞中CD3＋、CD56＋双阳性细胞的百分比为（45.3±5.1）％,与28 d相比较差异无统计学意义（P＞0.05）,第35天细胞增殖（212.50±4.25）倍,呈下降趋势.CIK细胞对结肠癌SW480细胞株有明显的杀伤作用,最高杀伤率为（68.21±1.31）％.结论：CIK细胞具有较强的抗结肠癌细胞活性,体外培养14～21 d具有较强的抗瘤活性,此时应用较为合适.CIK细胞作用于肿瘤细胞应达到一定数量并维持一定时间,可达到较好的抗瘤活性.     

蒸馏水与稀释碘伏对结肠癌细胞SW480体外增殖的比较研究

目的研究蒸馏水与稀释碘伏对人结肠癌细胞SW480体外增殖的抑制作用。方法体外培养人结肠癌细胞株SW480,分别用蒸馏水与不同浓度稀释碘伏（0.05%,0.025%,0.01%）处理,MTT（噻唑蓝）法检测蒸馏水与稀释碘伏对SW480细胞的生长影响作用,并进行分析评价。结果 0.01%碘伏处理细胞1 min即可表现出明显的细胞毒性作用,效果远远高于单纯使用蒸馏水的杀伤作用。结论稀释碘伏在体外对人结肠癌细胞SW480的杀伤作用明显高于单纯使用蒸馏水,可望为临床手术中控制腹腔结肠癌细胞种植转移提供临床依据。     

NK细胞联合西妥昔单抗对大肠癌细胞ADCC作用的研究

目的比较3株肠癌细胞株(LOVO、SW480、SW620)与自然杀伤(natural killer,NK)细胞、西妥昔单抗(Cetuximab)混合作用之后,NK细胞抗体依赖的细胞介导的细胞毒作用(antibody dependent cell cytotoxicity,ADCC),探讨Cetuximab抗肿瘤过程中ADCC的作用。方法免疫组织化学方法检测3种不同肠癌细胞系(LOVO、SW480、SW620)的EGFR表达水平;MTT法检测6种不同浓度Cetuximab作用48h后肿瘤细胞的生长抑制情况;NK细胞与Cetuximab包被的肿瘤细胞共孵育,LDH法检测NK细胞的ADCC功能变化。结果免疫组织化学结果显示,3株细胞EGFR表达:LOVO(),SW480(+),SW620(-);Cetuximab对LOVO细胞和SW480细胞的生长抑制呈剂量依赖,对SW620细胞无抑制作用;LOVO细胞和SW480细胞经Cetuximab处理后,NK细胞的ADCC作用均增强且有统计学意义(P<0.05)。结论单独的Cetuximab可抑制EGFR阳性的LOVO细胞和SW480细胞,而对EGFR阴性的SW620细胞生长抑制作用不明显,所选的2株EGFR阳性肿瘤细胞都可被Cetuximab所介导ADCC作用所抑制,高EGFR表达的肿瘤细胞更加敏感。     

阿司匹林对结肠癌细胞株SW480生长增殖和诱导凋亡作用及其机制研究

目的：研究阿司匹林对人结肠癌细胞株SW480生长增殖的影响和诱导凋亡作用及其机制。方法：以不同浓度（2.5、5.0、10.0mmol／L）的阿司匹林干预体外培养人结肠癌细胞株SW480，MTT比色法观察生长增殖，流式细胞仪检测细胞周期及凋亡率，透射电镜扫描观察细胞形态变化，免疫组化染色法检测凋亡相关基因。结果：阿司匹林对结肠癌细胞株SW480生长增殖具有抑制作用，阻滞细胞周期于G0／G1期，诱导细胞凋亡，降低Bcl-2基因表达，提高Bax基因表达，阿司匹林上述作用呈浓度和时间依赖关系。此外，高浓度阿司匹林可导致细胞坏死。结论：阿司匹林具有拮抗结肠癌细胞株的生长增殖作用，通过调控Bcl-2、Bax等基因表达而诱导细胞凋亡或细胞坏死，阻滞细胞周期可能是其抗肿瘤作用机制之一。     

紫花牡荆素对人结肠癌SW480细胞生长和凋亡的影响

目的观察紫花牡荆素（CAS）对人结肠癌SW480细胞生长和凋亡的影响。方法体外培养人结肠癌SW480细胞,平皿克隆形成法测定细胞锚定依赖性生长能力,PI染色流式细胞术（FCM）分析细胞凋亡率,Western Blotting分析CAS对上调死亡受体-5（DR5）、抑制核因子κB（NF-κB）（p65）蛋白表达的影响。结果平皿克隆形成法检测显示CAS呈浓度依赖性抑制SW480细胞生长。PI染色FCM结果表明CAS呈时间和浓度依赖性诱导SW480细胞凋亡。Western Blotting检测显示CAS呈时间和浓度依赖性上调死亡受体-5（DR5）表达和抑制核因子κB（NF-κB）活性。结论 CAS具有抑制人结肠癌SW480细胞增殖和诱导凋亡的作用,其诱发结肠癌细胞凋亡作用与DR5表达和NF-κB活性有关。     

姜黄素对人结肠癌细胞SW480增殖周期及凋亡的影响

目的探讨姜黄素对人结肠癌细胞SW480细胞生长的抑制作用及其对细胞增殖周期和凋亡的影响。方法采用甲基噻唑（MTT）比色法和生长曲线测定不同浓度姜黄素在不同作用时间内对在体外培养SW480细胞增殖的影响，同时应用流式细胞术检测细胞增殖周期及凋亡率的变化。结果姜黄素可在G0／G1期阻滞人结肠癌细胞SW480的增殖，使S期细胞比率降低；在一定范围内，姜黄素的浓度越高、作用时间越长，对肿瘤细胞生长的抑制作用越强，凋亡率也越高。结论姜黄素能抑制人结肠癌细胞SW480细胞生长，并诱导细胞凋亡而阻止细胞周期。     

丁酸钠对结肠癌细胞SW480凋亡及Survivin基因和VEGF表达水平的影响

目的:观察丁酸钠对结肠癌细胞株SW480的生长抑制、凋亡情况以及对生成素(Survivin)表达水平的影响,探讨其预防结肠癌的作用机制。方法:人传代结肠癌细胞株SW480经不同浓度丁酸钠处理后,在不同时段收集细胞,分别用四唑蓝法检测肿瘤细胞生长增殖率,流式细胞仪检测细胞周期和凋亡变化,免疫细胞化学技术观察对Survivin基因和血管内皮生长因子(VEGF)表达水平。结果:随着培养液中丁酸浓度的不断升高和培养时间的延长,SW480细胞的生长逐渐受到抑制。G1期细胞明显增多,S期细胞明显减少,细胞凋亡率逐渐增高。Sur-vivin基因和VEGF随着丁酸浓度的不断升高而表达下降。结论:丁酸钠能抑制SW480细胞株增生,诱导凋亡,并抑制Survivin基因和VEGF表达。     

辣椒素对人结肠癌SW480细胞株增殖作用的影响

目的 了解辣椒素对人结肠癌 SW480 细胞株增殖作用的影响,并探讨其可能的作用机制.方法 采用MTT检测法,观察终浓度100～400 μmol/L的辣椒素作用24、48 h对人结肠癌SW480 细胞株生长作用的影响;采用流式细胞仪检测400 μmol/L辣椒素作用人结肠癌 SW480 细胞株后其细胞周期变化和凋亡率.结果 辣椒素能抑制结肠癌 SW480 细胞株增殖,呈浓度、时间依赖性.在400μmol/L辣椒素作用下,结肠癌SW480细胞株停滞于G0/G1期.辣椒素作用24、48 h,SW480 细胞凋亡率升高(F=8.73、8.10,q=4.86～7.30,P<0.01).结论 辣椒素抑制 SW480 细胞增殖,并诱导其凋亡,其机制可能是阻滞细胞周期于G0/G1,期.     

吡罗昔康对人γδT细胞和消化系统肿瘤细胞的作用比较

目的:探讨吡罗昔康对人γδT细胞杀伤消化系统肿瘤细胞株的影响。方法:用异戊烯焦磷酸法体外扩增人外周血γδT细胞。用不同浓度的吡罗昔康诱导γδT细胞和消化系统肿瘤SGC-7901、SW-1990、SW-480、SW-1116、LOVO细胞株,用MTT法检测吡罗昔康对这些细胞的抑制率和用乳酸脱氢酶法测定γδT细胞的杀伤活性,用流式细胞术检测诱导前后的γδT细胞和SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞凋亡百分率。结果:γδT细胞培养10d时从扩增前4.21%增加到70.35%。吡罗昔康各种浓度对SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞株抑制率明显高于γδT细胞。0.01~0.04mmol/L吡罗昔康诱导24h后的γδT细胞对5种肿瘤细胞的杀伤活性最高,浓度超过0.04mmol/L时杀伤活性呈下降趋势;SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞经不同浓度的吡罗昔康诱导24h后γδT细胞对其的杀伤活性与对照组比较无明显变化。0.16mmol/L吡罗昔康诱导24h对SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞株的凋亡率分别为12.26%、13.46%、14.59%、25.64%和39.36%,显著高于γδT细胞(8.16%)。结论:吡罗昔康在临床常规使用的药物浓度时可增强γδT细胞杀伤肿瘤细胞作用,超过这一浓度可明显抑制γδT细胞和肿瘤细胞的增殖能力和杀伤活性及增加细胞的凋亡率;吡罗昔康对肿瘤细胞株的抑制率明显高于γδT细胞。这一结果为临床吡罗昔康预防消化道肿瘤的用量提供了实验依据。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.