生物活性双梯度羟基磷灰石涂层界面结合稳定性研究

[目的]检验生物活性双梯度羟基磷灰石涂层的界面结合强度和稳定性.[方法]将同一规格的3种不同植入体(Ti,HaTi,rhBmp-2HaTi)植入狗股骨,术后3、6、16周分别处死3组动物,通过顶出试验、扫描电镜等检查手段进行检测、观察.[结果]早期植入体的界面抗剪切强度比较Ti＜HaTi＜rhBmp-2HaTi;6、16周组rhBmp-2HaTi与HaTi植入体界面分离、破坏发生在新生骨内部和骨-涂层两部分,涂层内部无破坏.[结论]该种生物活性双梯度羟基磷灰石涂层具有很强的界面结合强度和界面结合稳定性.     

羟磷灰石涂层钛合金植入材料的实验研究

目的 通过测定一种新的羟磷灰石（HAP）涂层钛合金植入材料的理化参数、与宿主骨的生物相容性观察及骨－HAP界面最大剪切强度的测定，评估该材料作为人工骨或人工关节材料的临床应用价值。方法 （1）测定材料的最佳烧结温度和机械强度，观察材料表面的多孔结构。（2）取24只新西兰种家兔，在两侧股骨外髁上共植入48个HAP表面涂层及非涂层钛合金材料，分别于术后1、2、3、6及12周处死家兔取材。测定骨－HAP界面最大剪切强度并作组织学观察。结果 （1）材料的最佳烧结温度为800℃，抗剪强度为18.5MPa，抗压强度为170MPa，孔隙率为10％－15％，孔径为30－70μm。（2）HAP涂层钛合金组能与宿主骨紧贴，新骨能迅速长入材料的裂隙和被降解的封闭小孔内，而非涂层材料则使新生骨组织死亡并为结缔组织取代。（3）术后任一时间段涂层材料骨－HAP界面最大剪切强度均高于非涂层组。结论 这种新材料能作为人工骨或人工关节材料而应用于临床。     

羟基磷灰石涂层的钛-75合金种植体的犬骨内种植实验

目的：观察评体羟基磷灰石（ＨＡ）烧结涂层的钛０７５（Ｔｉ－７５）合金种植体（ＨＡＴＩ）的生物学性能与生物力学特性。方法：应用穿皮质骨种植模型,以纯钛种植体（ＰＴＩ）作对照,在双侧犬股骨内种植ＨＡＴＩ和ＰＴＩ共３２ 术后３个月和６个月处死动物取村。应用组织学、定量组织学、推出试验、扫描电镜等方法,观察种植体骨界面的结合情况和剪切强度。结果：ＨＡＴＩ与ＰＴＩ均与骨组织形成了骨性结合,ＨＡＴＩ的骨性结合     

钛种植体－羟基磷灰石早期修复犬下颌骨缺损与骨结合的实验研究

本研究对钛种植体－羟基磷灰石（Ｔｉ－ＨＡ）在早期修复下颌骨缺损中与骨结合的界面形态进行了扫描电镜和Ｘ线能谱观察分析。通过对９只健康狗下颌骨缺损区植入Ｔｉ－ＨＡ２、６及１２周后Ｔｉ－ＨＡ／宿主骨界面及Ｔｉ／ＨＡ界面的扫描电镜观察和Ｘ线能谱分析，结果表明Ｔｉ－ＨＡ植入骨缺损区，不引起正常的骨矿化过程改变，与宿主骨形成骨性结合；Ｔｉ／ＨＡ界面有新骨形成；扫描电镜观察和Ｘ线能谱分析在研究骨缺损修复过程中不同阶段的组织成份的变化是一个重要手段。     

钛种植体—羟基磷灰石早期修复犬下颌骨缺损与骨结合的实验研究

本研究对钛种植体－羟基磷灰石（Ｔｉ－ＨＡ）在早期修复下颌骨缺损中与骨结合的界面形态进行了扫描电镜和Ｘ线能谱观察分析。通过对９只健康狗下颌骨缺损区植入Ｔｉ－ＨＡ２、６及１２周后Ｔｉ－ＨＡ／宿主骨界面及Ｔｉ／ＨＡ界面的扫描电镜观察和Ｘ线能谱分析，结果表明Ｔｉ－ＨＡ植入骨缺损区，不引起正常的骨矿化过程改变，与宿主骨形成骨性结合；Ｔｉ／ＨＡ界面有新骨形成；扫描电镜观察和Ｘ线能谱分析在研究骨缺损修复过程中不     

羟基磷灰石涂层种植体植入生物体内结合过程的动物实验观察

目的了解羟基磷灰石（ＨＡ）涂层种植体植入生物骨组织后的愈合情况,为义齿修复时机提供依据。方法将表面涂ＨＡ的钛芯种植体植入狗颌骨内,分别在术后３、６、１２个月于全麻下切取标本,在光镜和电镜下观察其结合情况。结果ＨＡ涂层种植体植入狗颌骨后３、６个月即形成骨性结合界面,超过六个月其愈合程度与时间无关。结论ＨＡ涂层种植入生物体后３～６个月即形成骨性结合界面,可进行上部修复。     

HA涂层种植体即刻种植的临床观察

观察ＨＡ涂层种植体即刻种植的治疗效果,探讨ＨＡ涂层种植体在即刻种植术中临床应用的前景。方法对２６例因外伤,残酹,牙周病等原因需拔除患牙的思考,在拔除患牙的同时即刻植入３６枚国产ＨＡ涂层种植体,种植体与拔牙创间较大的骨间隙用ＨＡ颗粒充填或胶原膜覆盖以引导骨再生,４－６个月后二次手术,连接上部结构并完成修复。修复后对种植体稳固性,牙槽嵴丰满度、骨吸收、骨融合情况等进行临床和Ｘ线的连续观察。结果２６例３     

功能性梯度纳米HA复合涂层促进早期骨生成

采用犬股骨髁植入功能性纳米羟基磷灰石（nanophase hydroxyapatite，n-HA）功能性梯度材料涂层栓和传统材料等离子喷涂涂层（plasma sprayinyhy droxyapatite,PS-HA）栓，并使用四环素荧光双标记。分别在4、8和12周，取材植入体-骨界面组织，制备硬组织切片，通过光学显微镜、荧光显微镜以及图像分析软件，比较两种材料涂层植人体-骨界面成骨细胞生成、植入体界面骨结合情况等组织学情况，通过测量计算植入体界面骨矿化沉积率等骨动力学参数以及涂层材料降解情况，对功能性n-HA复合梯度材料的生物学特性进行了研究。结果表明功能性n-HA复合梯度材料植入体界面较早出现大时成骨细胞，新骨生成速度较快，与植入体紧密结合，材料降解速度慢于传统羟基磷灰石组，骨动力学参数优于对照组。功能性n-HA复合梯度材料具有良好的生物学特性，能促进植入体界面的早期结合，并具有改善植入体远期固定宦效果的潜力，是一种理想的生活学植入材料，具有一定的临床应用前景。     

羟基磷灰石涂层骨内牙种植体即刻与延期种植的动物实验研究

目的 观察国产羟基磷灰石涂层钛基种植体（ＨＡＣＩ）在即刻与延期种植后植入区骨组织反应。方法 模拟临床延期和有或无羟基磷灰石颗粒（ＨＡＧ）充填即刻种植术，在狗股骨植入ＨＡＣＩ，种植后１，２，３，４个月时制作植入区骨组织不脱钙标本切片，进行组织学观察，显微Ｘ线摄影及计算机辅助种植体－新生骨接触率测量。结果 新生骨组织在种植后１月时即已覆盖了种植体的大部分表面；３种种植方法在种植后的同一阶段新生骨与种植     

骨形成蛋白复合钛心我孔羟基磷灰石涂层种植体的临床疗效研究

用骨形成蛋白（ＢＭＰ）与钛心多孔羟基磷灰石（ＨＡ）涂层种植体复合，制成ＢＭＰ－ＨＡ复合种植体，在１０例病人１５个缺牙区用１５枚该复合种植体进行种植；同时对条件相似的１１例病人的缺牙植入１５枚ＨＡ涂层种植体作为对照。追踪观察每枚种植体的不同期Ｘ线牙片、种植体的松动度、判定种植周围的新骨修复情况，结果发现：ＢＭＰ－ＨＡ复合种植体周转的新骨形成速度和成骨量高于ＨＡ涂层种植体。同时发现，种植４周后，两组患     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…