甜茶素对小鼠糖异生作用和血脂代谢的影响1

目的研究甜茶素对小鼠糖异生作用和血脂代谢的影响.方法雄性昆明种小鼠22只,随机分为对照组和给药组.给药组小鼠每天灌胃甜茶素,对照组小鼠灌胃生理盐水.灌胃3周后,测小鼠的血清葡萄糖、血清甘油三酯、血清胆固醇及肝糖原等生化指标.结果甜茶素能降低正常小鼠血糖水平,降糖率为18.47%;其对小鼠糖异生具有明显的抑制作用,抑制率为17.32%.同时,甜茶素能明显降低血清甘油三酯水平,降低幅度为30.08%;对胆固醇含量也有降低作用.结论甜茶素对小鼠血糖代谢的影响与控制糖异生途径有关.     

沙棘籽渣和果渣中黄酮对小鼠糖代谢的影响

目的：研究沙棘籽渣和果渣黄酮对小鼠糖代谢的影响。方法：健康雄性昆明种小鼠随机分为对照组，沙棘籽渣黄酮(FSH)低、中、高剂量组和沙棘果渣黄酮(FFH)低、中、高剂量组，对照组小鼠灌胃生理盐水，其余各组小鼠每天灌胃相应药物。灌胃7d、14d、16d时，测小鼠的血糖、血脂水平；16d后，作糖异生试验，同时作肝糖原分析。结果：正常小鼠灌胃FSH或FFH后，血糖和血脂水平低于对照组；同时对小鼠糖异生具明显的抑制作用。结论:FSH和FFH能降低正常小鼠的血糖和血脂水平,且对血糖代谢的影响与控制糖异生有关。     

鬼针草提取物对高脂高糖模型小鼠的影响

目的:研究鬼针草提取物对小鼠高脂血糖的影响。方法:健康SPF级小鼠随机分为6组:正常对照组、模型对照组、阳性对照组、鬼针草醇提物组(高、中、低);正常对照组与模型组每天灌胃蒸馏水;高、中、低浓度组分别按160 mg/kg、80 mg/kg、40 mg/kg的剂量灌胃给药,阳性对照组给予二甲双胍加脂必妥。连续喂养4周,在最后一次给药后,禁食12 h,断尾采集血液、然后处死取肝脏。制备肝脏病理切片,HE染色观察形态学变化。结果:研究发现,与模型组相比,阳性对照组和鬼针草醇提物(TFB)高、中、低给药组灌胃给药可显著降低高血脂高血糖小鼠血糖水平、低密度脂蛋白(LDL-C)、甘油三酯(TG)、血清总胆固醇(TC)的含量,升高高密度脂蛋白(HDL-C)的含量,提示TFB具有显著的降低血糖、血脂的作用。TFB给药组和阳性给药组小鼠的肝细胞脂肪变性程度均较模型组病理片有明显改善。结论:以上实验结果表明,TFB可明显降低高脂高血糖小鼠的血糖、血清的TG、TC、LDL-C,升高HDL-C,调节脂质代谢,对肝组织脂肪变性有显著改善作用。     

藏药复方TKF对自发性2型糖尿病小鼠(KK-Ay小鼠)糖代谢的作用研究

目的:研究藏药复方TKF对KK-Ay小鼠的降糖作用。方法:以C57BL/6 J小鼠作为对照组,KK-Ay小鼠随机分为模型组、TKF组(1.125、2.25、4.5g/kg)和格列本脲组5组,连续灌胃35天,检测小鼠体重、空腹血糖、口服糖耐量、血清胰岛素和血脂等指标。结果:TKF 2.25g/kg组从给药7天起即出现空腹血糖的降低,并在给药期间维持在一较低水平,给药35天时TKF各组血清胰岛素水平显著降低,2.25g/kg组血清甘油三酯亦明显降低。结论:TKF具有降低KK-Ay小鼠血糖、血脂和胰岛素水平,改善胰岛素抵抗的作用。     

蒺藜皂苷的降血糖作用

目的:研究蒺藜皂苷的降血糖作用。方法:以四氧嘧啶(Alloxan)致糖尿病小鼠,以降糖灵为阳性对照药,灌胃10 d后,测定小鼠血清葡萄糖、甘油三酯、胆固醇及超氧化合物歧化酶(SOD)等生化指标。结果:蒺藜皂苷能显著降低正常小鼠和Alloxan致糖尿病小鼠的血糖水平,降糖率分别为26.25％和40.67％;蒺藜皂苷在降低血糖的同时,能够显著降低糖尿病小鼠血清甘油三酯水平,其降低率为23.35％,对血清胆固醇含量也有一定的降低趋势;同时蒺藜皂苷还能够显著提高血清SOD活力。结论:蒺藜皂苷具有明显的降血糖作用。     

蒺藜水煎剂对小鼠糖代谢中糖异生的作用

蒺藜水煎剂能够明显抑制正常小鼠的糖异生作用,影响糖代谢;同时能够明显抑制糖异生小鼠血清甘油三酯水平,降低幅度为27．82％;对血清胆固醇含量也有明显的降低作用。     

芒果苷对糖尿病小鼠糖脂代谢的影响

目的:观察多酚类化合物芒果苷对四氧嘧啶性糖尿病小鼠糖脂代谢的调节作用,并探讨其作用机制。方法:采用雄性ICR小鼠尾静脉一次性注射四氧嘧啶(60.0 mg/kg)诱导糖尿病小鼠动物模型。芒果苷(12.5、25、50mg/kg)腹腔注射给药4周,测定各组糖尿病小鼠血糖、血脂、糖原和血清胰岛素的水平;通过对小鼠胰腺的H.E染色和胰岛B细胞的醛品红染色,观察芒果苷对胰岛和B细胞的影响。结果:芒果苷腹腔注射给药4周后,25、50mg/kg芒果苷组能显著降低糖尿病小鼠血糖,并增加其肌、肝糖原含量;50mg/kg芒果苷组糖尿病小鼠血清胰岛素水平、胰岛和B细胞的数量显著高于糖尿病模型组。12.5、25、50mg/kg芒果苷能显著降低糖尿病小鼠血清甘油三酯和总胆固醇含量。结论:芒果苷通过促进糖尿病小鼠肌、肝糖原的合成和胰岛素的分泌,降低血清甘油三酯和总胆固醇含量来调节糖尿病小鼠的糖脂代谢。     

阔叶十大功劳根提取物对糖尿病模型小鼠降糖作用研究

目的:研究阔叶十大功劳根提取物对链脲佐菌素(STZ)诱导2型糖尿病模型小鼠的降血糖作用。方法:将小鼠分为对照组、模型组、二甲双胍组、水提物组、醇提物组,采用腹腔注射链脲佐菌素(STZ)建立2型糖尿病小鼠模型。连续灌胃相应药物4周,每周检测小鼠体重和血糖变化。灌胃结束后,摘眼球取血并立即取出肝脏待测。采用ELISA法测定血清中总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇水平(LDL-C)水平,血清和肝脏中超氧化物歧化酶(SOD)、丙二醛(MDA)、还原型谷胱甘肽(GSH)。结果:与模型组比较,阔叶十大功劳根水提物、醇提物给药1周后能显著降低糖尿病小鼠空腹血糖水平;给药4周后,与模型组比较,醇提物组小鼠血糖水平极显著降低。阔叶十大功劳根水提物、醇提物能有效降低2型糖尿病小鼠血清中总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)水平,显著升高高密度脂蛋白胆固醇(HDL-C)水平,显著降低血清和肝脏中过氧化物MDA水平,显著升高血清和肝脏中SOD和GSH水平。结论:阔叶十大功劳根提取物对链脲佐菌素诱导的实验性糖尿病小鼠具有保护作用,其机制可能与降低小鼠血脂水平和提高机体的抗氧化能力有关。     

金银花提取物对血脂与血糖的影响

目的:研究金银花提取物对动物血脂、血糖的影响。方法:建立高脂血症和糖尿病动物模型,分组灌胃给药并且测定高脂血症模型动物血清胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、肝组织中TC、TG含量以及糖尿病模型动物血糖含量。结果:金银花提取物Ⅰ(4、2g/kg)可使高脂血症小鼠、大鼠血清及肝组织TG水平明显降低,对TC、LDL-C、HDL-C无明显影响;其余各组及提取物Ⅱ对血脂、肝脂无降低作用。金银花提取物Ⅰ和Ⅱ可降低蔗糖性高血糖小鼠和四氧嘧啶糖尿病模型小鼠血糖。结论:金银花提取物有一定降血脂、血糖作用,其有效成分和作用机制有待进一步研究。     

三黄制剂降血脂作用研究

目的：观察三黄制剂对实验小鼠血清中总胆固醇和甘油三酯含量的影响。方法：给小鼠灌服高脂乳剂和治疗药,正常组的给以等量蒸馏水,连续给药10d。末次给药16h后,眼眶采血,分别测定血清中总胆固醇和甘油三酯。结果：三黄制剂高,低剂量组均能不同程度地降低小鼠血清中总胆固醇和甘油三酯。结论：D一黄制剂能降低实验小鼠血清中总胆固醇和甘油三酯的含量。     

COMBINED TREATMENT OF 198 CASES OF INSOMNIA WITH ACUPUNCTURE OF BODY ACUPOINTS PLUS PELLET PRESSING OF OTOPOINTS

Objective: To observe the therapeutic effect of acupuncture of body acupoints plus otopoint pelletpressing for treatment of insomnia. Methods: A total of 198 cases of insomnia patients were observed. Straight inserted the filiform needles into Shenmen (HT 7), Neiguan (PC 6), Zhaohai (KI 6) and Zusanli (ST 36) and manipulated the needles with uniform reinforcing-reducing method, then otopoints as Ershenmen (MA-TF 1 ), Shen (MA-SC), Gan (MA-SC 5), etc. were stimulated by pressing the stuck vaccaria seeds. Ten treatments were considered as a therapeutic course. Results: Of the 198 cases, 65 (32%) were cured, 76 (38%) had remarkable improvement, 53 (26%) had improvement and 4 (2%) had no effect, with the total effective rate being 97% Conclusion:Acupuncture of body acupoints plus otopoint pellet pressing works well in treatment of insomnia.     

TREATMENT OF 247 CASES OF PROLAPSE OF LUMBAR INTERVERTEBRAL DISC WITH COMPREHENSIVE PLUM-BLOSSOM MAGNETIC NEEDLE THERAPY

Objective: To.observe the therapeutic effect of comprehensive plum-blcssom magnetic needle therapy on prolapse of lumbar intervertebral disc. Methods. 247 cases of prolapse of lumbar intervertebral disc were treated first with manual treatment and then with plum-blcssom magnetic needle therapy and effect-increasing pad therapy at specified points and reactive points. Results: After treatment for 2 therapeutic courses, 153 cases were cured accounting for 61.94%, 71 cases were markedly effective accounting for 28.75%, 22 cases were improved accounting for 8.91% and the rest one failed,acceunting for 0.45%. Conclusion. The comprehensive plum-blessom magnetic needle therapy has a better therapeutic effect on prolapse of lumbar intervertebral disc.     

COMPARISON OF THE THERAPEUTIC EFFECT OF AURICULAR ACUPUNCTURE, BODY ACUPUNCTURE AND COMBINATION OF AURICULAR PLUS BODY ACUPUNCTURE FOR TREATMENT OF SIMPLE OBESITY

1 CLINICAL DATA1. l Subjects: All the patients were from theOut-patient Department (SPecialist Clinic foracupuncturesreducing body weight) of NanjingTCM and Pharmacy University and from theout-patient department (Specialist Clinic forAcupuncture-reducing Body Weight) of our hos-pital. They were diagnosed as simple obesitythrough clinical examination.l. 2 General data: In this series of treatment,l95 cases of simple obesity including 8 males andl87 females were observed. The oldest …     

膝关节骨关节炎患者证候特点分析--附624例分析

为探讨膝关节骨关节炎患者证候类型及其诊断要点,收集624例确认为膝关节骨关节炎患者的四诊资料,采用症状计量辨证法辨析基本证候,聚类分析法归纳证候类型,二值多元Logistic回归分析法确定各证候的辨证要点.结果显示624例膝关节炎患者的基本证候分别为:肾阳虚证508例(81.4%),血瘀证464例(74.4%),寒湿证340例(54.4%),肝阴虚证292例(46.8%),脾虚证212例(34.0%),肾阴虚证120例(19.2%),痰证48例(7.7%),湿热证40例(6.4%),风证4例(0.6%).证候类型的聚类分析结果为肾阳虚血瘀寒湿证276例(44.2%),肝阴虚肾阳虚血瘀证156例(25.0%),肝脾肾虚血瘀证104例(16.7%),肝肾阴虚血瘀证88例(14.1%),并归纳出各证型的辨证要点.表明肾阳虚证、血瘀证、寒湿证、肝阴虚证、脾虚证、肾阴虚证、痰证、湿热证、风证等是膝关节骨关节炎的基本证候单位,肾虚寒湿瘀滞证、肝肾亏虚血瘀证、肝脾两虚肾虚血瘀证、肝肾阴虚血瘀证等是膝关节骨关节炎的常见中医证候类型.     

STUDY OF EFFECTIVENESS OF ACUPUNCTURE TREATMENT ON HYPERPLASIA OF MAMMARY GLANDS

Objective: To observe whether there is an improvement in breast pain, swelling or breast ultrasound after acupuncture treatment and to compare the results with the effects of widely used medicines such as dairy Acbel capsule.Methods: A total of 160 research-suitable HMG patients were selected through the Beijing University of Chinese Medicine Oriental Hospital's Galactophore Department and Acupuncture Branches.Totally 153 patients completed the study according to the protocol.The patients were randomly categorized into two groups.One group was treated by acupuncture and the other by Chinese medicine.The acupuncture group selected Danzhong, Wuyi, Rugen, Qimen, and Sanyinjiao acupoints for treatment.There were a total of 15 treatments for each patient and each treatment lasted for 20 min.Treatments were not carried out during the patients' menstrual period.Patients in the Chinese medicine group were prescribed three orally ingested dairy Acbel capsule three times a day.The medicine was not taken during the patients' menstrual period.Both groups started their respective treatments during their luteal phase and the effectiveness of treatment was evaluated during their next luteal phase.Results: In the acupuncture group, 15.1% of the patients had successful treatments and 83.6% of the patients had improvement in their symptoms, whereas for the Chinese medicine group, 19.0% of the patients had successful treatments and 83.5% saw improvement in their conditions.There was no statistically significant difference between the two groups(P0.05).The treatments used for both groups significantly alleviated breast pain and reduced swelling among HMG patients.Conclusion: Acupuncture treatment can improve the conditions of HMG patients significantly.The treatment effect is positive and the effectiveness is equivalent to that of the dairy Acbel capsules.This method is simple and convenient, free of harmful side effects, relatively inexpensive, and suitable for clinical promotion.     

OBSERVATION ON THE THERAPEUTIC EFFECT OF ACUPUNCTURE TREATMENT OF 123 CASES OF SCAPULOHUMERAL PERIARTHRITIS

Ｓｃａｐｕｌｏｈｕｍｅｒａｌｐｅｒｉａｒｔｈｒｉｔｉｓｉｓｏｎｅｏｆｔｈｅｃｏｍｍｏｎｌｙｓｅｅｎｄｉｓｅａｓｅｓｉｎｃｌｉｎｉｃａｎｄｉｓａｎｉｎｄｉｃａ ｔｉｏｎｏｆａｃｕｐｕｎｃｔｕｒｅｔｈｅｒａｐｙ .Ｔｈｉｓｄｉｓｅａｓｅｉｓｒｅ ｆｅｒｒｅｄｔｏｔｈｅｒｅｔｒｏｇｒａｄｅａｆｆｅｃｔｉｏｎａｎｄｓｔｒａｉｎｉｎ ｄｕｃｅｄｗｉｄｅｓｐｒｅａｄｃｈｒｏｎｉｃａｓｅｐｔｉｃｉｎｆｌａｍｍａｔｉｏｎａｒｏｕｎｄｔｈｅｓｈｏｕｌｄｅｒｊｏｉｎｔａｎｄｉｔｓｓｕｒｒｏｕｎｄｉｎｇｓｏｆｔｔｉｓｓｕ…     

CLINICAL OBSERVATION OF TREATMENT OF HEMIPLEGIA AFTER ISCHEMIC STROKE BY ACUPUNCTURE OF REGULATING YIN-YANG BALANCE PENETRATION

Objective: To observe the clinical efficiency of the treatment on apoplexy by acupuncture of regulating yin-yang balance penetration, and to seek the effective methods and mechanism of the treatment on hemiplegia after ischemic stroke.Methods: Ninety cases of the ischemic stroke were randomly divided into three groups by using central random system: acupuncture and rehabilitation group(n=30), acupuncture group(n=30) and rehabilitation group(n=30).The patients of each group were treated based on corresponding acupuncture or rehabilitation treatment, followed by antihypertensive, regulating lipid and other symptomatic supportive treatment on the condition that the professional evaluator did not know which group the patient belonged to.The evaluators assessed the measuring score of NDS FMA 28 d after treatment.Results: The total effective rate of the acupuncture and rehabilitation group was 86.7% while the acupuncture group was 80%, and both were better than 70% of the rehabilitation group(P0.01); in reducing neurological deficit scores, the observation group was better than the control group(P0.01).Conclusion: The method of acupuncture of regulating yin-yang balance penetration is an effective method.It has a great prospective of use.     

IMMEDIATE RELIEF OF KNEE PAIN THROUGH THE COMPARISON OF HAND AND EAR ACUPUNCTURE

Objective: The present study aimed to assess the immediate effect of knee pain relief by comparing two techniques, the Koryo Sooji Chim(hand acupuncture) and ear acupuncture.Methods: Forty subjects were included in the present study and randomly divided into 4 groups of 10 subjects in each: real hand acupuncture, sham hand acupuncture, real ear acupuncture and sham ear acupuncture.Each patient was evaluated by Visual Analog Scale(VAS) before and after the stimuli.Results: In group A, subjects who underwent real hand acupuncture showed 50%(5 individuals) improvement in pain and 50%(5 individuals) unchanged; in group B, subjects who underwent sham hand acupuncture showed 100%(10 individuals) unchanged; in group C, subjects who underwent real ear acupuncture showed 90%(9 individuals) improvement in pain and 10%(1 individual) did not change; and in group D, subjects who underwent sham ear acupuncture showed no changes in 100%(10 individuals).Conclusion: From the results, it is clear that the correct application of the stimulus shows better improvement in pain, with the prevalence rates of ear acupuncture that showed better efficacy compared to Korean hand acupuncture.     

电针对糖尿病胃轻瘫大鼠胃Cajal间质细胞超微结构的影响

目的：观察电针对糖尿病胃轻瘫（D（碧）大鼠胃Cajal间质细胞（ICC）超微结构的影响。方法：采用腹腔一次性注射2％链脲佐菌素溶液制备糖尿病模型。灌服200％熟地水煎液复制DGP模型，分组给予相应处理。电子显微镜下观察ICC的超微结构变化。结果：电针及吗丁啉均能增加ICC数量，并且修复其受损的超微结构。结论：ICC变性是DGP发生的一个重要原因，电针可以增加ICC的数量及修复ICC的受损结构，进而恢复其功能，从而起到治疗DCP的作用。     

CLINICAL OBSERVATION ON TREATMENT OF 61 CASES OF INSOMNIA WITH AURICULAR PLASTER THERAPY

Objective: To observe the therapeutic effect of auricular plaster (otopoint-pressure) therapy in the treatment of insomnia. Methods: In this paper, 61 cases of insomnia patients including 16 males and 45 females were treated with Ershenmen (MA-TF 1), Zhen (MA-AT), Yuanzhong (MA-AT), Nao Dian and E (MA-AT), confined with other otopoints according to symptoms. The otopoint was stuck with a piece of plaster adhered with vaccaria seeds and pressured by the patient him- or her-self. The treatment was given twice a week, with 7 times being a therapeutic course. Results. After 1-2 courses of treatment, 19 (31.15%) cases were cured, 34 (55.74%) had improvement and 8 (13.11%) had no ~, with the total effective rate being 86.9%. Conclusion: Auricular plaster therapy works well in the treatment of insomnia patient.