抗氧化剂对皂苷溶血作用的影响

目的 研究麦冬总皂苷、人参总皂苷和生脉散的溶血作用及抗氧化剂对溶血的影响。方法 以氰化高铁血红蛋白法测定血红蛋白含量结合红细胞形态学观察。结果 人参总皂苷不溶血,麦冬总皂苷和生脉散溶,血溶血强度与皂苷浓度呈剂量依赖性,加入皂苷后红细胞有形态学改变。葡萄糖及抗氧化剂甘露醇和VitE能减轻皂苷的溶血作用。结论 皂苷溶血作用强弱与其所含皂苷的种类有关,并部分与增强红细胞膜的脂质过氧化有关。     

金银花与山银花的体外溶血作用分析

目的:研究金银花和山银花的体外溶血作用,评价其潜在溶血风险。方法:以皂苷单体化合物、金银花有效部位及金银花、山银花药材等为实验材料,采用紫外分光光度法测定各类样品在不同浓度时对兔血红细胞的溶血率,绘制溶血率-质量浓度曲线。结果:忍冬苦苷A,忍冬苦苷B,灰毡毛忍冬皂苷甲,川续断皂苷乙和川续断皂苷Ⅵ在质量浓度≤1.0 g·L~(-1)时均未发生溶血。灰毡毛忍冬皂苷乙的溶血率随着质量浓度的升高而增大,当质量浓度≤0.6 g·L~(-1)时未发生溶血;当质量浓度为0.8 g·L~(-1)时,溶血率上升至11.7%;当质量浓度为1.0 g·L~(-1)时,溶血率达到50.4%。金银花各有效部位溶血作用的相对强弱为金银花总皂苷部位总酚部位总环烯醚萜部位。其中,金银花总环烯醚萜部位在质量浓度为0.1~1.2 g·L~(-1)时未发生溶血;金银花总酚部位质量浓度1.0 g·L~(-1)时,未发生溶血。金银花总皂苷部位在质量浓度为0.6 g·L~(-1)时开始溶血,之后随着质量浓度的升高,其溶血率急剧增大,当质量浓度为1.2 g·L~(-1)时,溶血率55.3%。金银花野生品、栽培品与山银花的溶血率-质量浓度曲线较为相似,其溶血率急剧增大的质量浓度为2.0~4.0 g·L~(-1)。结论:金银花与山银花的溶血作用无显著性差异;各种皂苷单体化合物没有溶血作用或溶血作用较弱;金银花总皂苷部位的质量浓度为0.6 g·L~(-1)(相当于生药质量浓度20 g·L~(-1))时具有较弱的溶血作用。尽管如此,中药注射液一般不会达到如此高的药物浓度。因此,含有皂苷类成分的金银花与山银花可应用于中药注射剂,其潜在的溶血风险较小。     

七叶一枝花和滇重楼提取物的制备、表征及其体外溶血作用分析

目的:比较七叶一枝花和滇重楼提取物体外溶血作用强弱,探讨不同结构类型重楼皂苷的溶血作用特点。方法:采用75%乙醇对七叶一枝花和滇重楼根茎进行提取,采用大孔吸附树脂对各类物质进行富集,采用快速制备色谱技术对目标组分群进行定向制备,基于对照品对照和在线紫外吸收光谱对总取物、部位和组分进行化学信息表征,采用分光光度法测定各样品的溶血率。结果:根据质量浓度-溶血率曲线计算七叶一枝花和滇重楼总提物的50%溶血浓度分别为34.97,46.52 mg·L~(-1)。在2.5~10 mg·L~(-1)时,七叶一枝花部位中仅有70%乙醇洗脱部位(PPC-D)显示了较强溶血作用,滇重楼部位中70%乙醇和95%乙醇洗脱部位(PPY-D和PPY-E)均显示溶血作用。5.0,10 mg·L~(-1)时PPC-D的溶血率分别为78.00%和95.68%,PPY-D(PPY-E)的溶血率分别为31.86%和63.29%(15.82%和55.25%)。在2.5~5.0 mg·L~(-1)时,七叶一枝花的组分呋甾皂苷和薯蓣皂苷未显示溶血作用,偏诺皂苷组分在质量浓度为2.5,5.0 mg·L~(-1)的溶血率分别为5.89%和90.33%;滇重楼的呋甾皂苷组分同样未显示溶血作用,偏诺皂苷(薯蓣皂苷)组分在质量浓度为2.5,5.0 mg·L~(-1)的溶血率分别为0.51%和57.75%(4.29%和49.39%)。结论:七叶一枝花和滇重楼均具有溶血作用,其中七叶一枝花强于滇重楼;七叶一枝花溶血成分群主要为偏诺皂苷类,滇重楼溶血成分群为偏诺皂苷和薯蓣皂苷;两者呋甾皂苷均无溶血作用;总提物溶血作用强弱主要由其中所含偏诺皂苷和薯蓣皂苷类成分的绝对含量和相对比例来决定。     

重楼皂苷类化合物溶血作用研究

目的 通过研究重楼皂苷类化合物的溶血作用,为重楼的进一步开发和利用提供依据.方法 以分离纯化获得重楼总皂苷、偏诺皂苷PHAC-A和薯蓣皂苷PHAC-B为实验材料,经梯度稀释后用常规肉眼观察法和分光光度法测定样品对兔血红细胞的溶血度.结果 当重楼总皂苷和(PHAC-A)在浓度大于等于50 μg·ml-1时、 (PHAC-B)浓度大于等于75 μg·ml-1时溶血率＞5%,3种样品的溶血作用均表现出剂量依存关系.结论 重楼皂苷中主要产生溶血作用的化合物为PHAC-A.重楼皂苷类化合物在特定浓度下不具有溶血作用,将其运用于静脉注射具有一定的可能性.     

人参皂苷溶血及抗溶血作用研究

目的：通过对几种单体人参皂苷溶血、抗溶血作用进行研究，得到人参皂苷浓度与溶血度间的关系曲线，探讨其作用规律。方法：不同浓度的单体人参皂苷溶液和相同浓度的兔血红细胞悬浊液组成的混合溶液充分作用后，用比色法测定溶血度；不同浓度的单体人参皂苷溶液与相同浓度的对照皂苷溶液混合后加入相同浓度的兔血红细胞悬浊液，三者组成的混合溶液充分作用后，用比色法测定溶血度并计算保护溶血百分数。通过数理统计软件对数据进行分析并拟合人参皂苷溶血或保护溶血作用的曲线。结果：在测定的人参皂苷各单体中分别或兼具有溶血和抗溶血的特性，且溶血和抗溶血能力各不相同。结论：原人参皂苷三醇型人参皂苷Re、Rg1、20（R）-Rg：、20（S）-Rg2和Rh。都具有抗溶血作用，其中20（R）-Rg2、20（S）-Rg2和Rh1，在较高浓度时还表现出溶血作用；原人参皂苷二醇型人参皂苷Rb1、Rb2、Rc和Rd均表现出抗溶血作用，其中Rd还在浓度较高时表现出了溶血作用。齐墩果酸型人参皂苷Ro在较低浓度便表现出抗溶血作用，未见溶血作用。     

人参皂苷Rgl的体外溶血作用研究

目的:考察人参皂苷Rgl的溶血作用.方法:参照2010版<中国药典>中"溶血"检查法.结果:1%、2%、3%的人参皂苷Rgl对红细胞无溶血作用.结论:长期大剂量静脉给人参皂苷Rgl还需谨慎.     

北柴胡总皂苷提取工艺研究

目的:优选从北柴胡中提取总皂苷的最佳工艺。方法:以总皂苷含量为指标,采用正交试验,考察了乙醇浓度、乙醇用量、提取次数和回流时间对总皂苷得率的影响。结果:总皂苷最佳工艺为:70%乙醇浓度,溶剂用量为药材的12倍,回流提取90min,提取3次。结论:该工艺简单可行,是提取北柴胡总皂苷的有效途径。     

柴胡总皂苷对小鼠抑郁样行为及学习记忆障碍的改善作用

目的:研究柴胡总皂苷连续给药抗抑郁作用起效时间及作用维持时间,以及柴胡总皂苷对皮质酮损伤引起的小鼠抑郁样行为及认知功能障碍的影响。方法:ICR雄性小鼠随机分为正常组(蒸馏水)、柴胡总皂苷低剂量组(12.5 mg·kg~(-1)),柴胡总皂苷中剂量组(25 mg·kg~(-1)),柴胡总皂苷高剂量组(50 mg·kg~(-1))。每周称体重,给药13 d后,进行空场实验。给药14 d后1 h,3 d,6 d观察柴胡总皂苷对强迫游泳实验不动时间的影响。C57BL/6J雄性小鼠随机分为正常组,皮质酮组(CORT组),西酞普兰组(10 mg·kg~(-1))和柴胡总皂苷(25 mg·kg~(-1))组。CORT组、西酞普兰组和柴胡总皂苷组饮用皮质酮。造模3周后,ig给予柴胡总皂苷,给药时间为2周,至第5周结束,西酞普兰ig,给药时间同柴胡总皂苷。给药结束后1 d,进行强迫游泳实验和水迷宫实验。行为学实验结束后,各组各取3只小鼠,断头,取海马,蛋白质免疫印迹(Western blot)进行脑源性神经营养因子(BDNF)和酪氨酸激酶B(Trk B)蛋白表达检测。结果:柴胡总皂苷连续给药14 d,与正常组比较,对体重和空场实验运动路程及速度无影响。停药后1 h和3 d,柴胡总皂苷各剂量组均可缩短小鼠强迫游泳不动时间(P0.01,P0.05);停药后6 d,柴胡总皂苷25,50 mg·kg~(-1)仍可缩短小鼠强迫游泳实验不动时间(P0.05)。柴胡总皂苷25 mg·kg~(-1)连续给药14 d,可缩短皮质酮损伤小鼠强迫游泳不动时间(P0.01),缩短水迷宫实验寻台潜伏期(P0.05),提高穿台次数(P0.05),并增加皮质酮损伤小鼠海马BDNF和Trk B蛋白表达(P0.01)。结论:柴胡总皂苷连续给药产生较快而持久的抗抑郁作用,逆转皮质酮损伤引起的小鼠抑郁样行为及学习记忆障碍。其作用机制可能与增加小鼠海马BDNF及Trk B表达有关。     

柴胡总皂苷提取工艺研究

目的：优选从柴胡中提取总皂苷的最佳工艺。方法：以总皂苷含量为指标,采用正交试验,考察乙醇浓度、乙醇用量、提取次数和回流时间对总皂苷得率的影响。结果：总皂苷最佳工艺为：75％乙醇,溶剂用量为药材的8倍,回流提取90min,提取2次。结论：该工艺简单可行,是提取北柴胡总皂苷的有效途径。     

三七总皂苷心脑血管药理作用及其溶血反应

三七总皂苷(total saponins of Panax notognseng,PNS)对于心脑血管疾病具有明确的治疗效应和广泛的应用前景,同时皂苷所具有溶血特性与其分子结构类型及用药剂量有关。该文一方面总结了近5年来PNS在心脑血管药理方面的研究进展;从整体、器官、细胞、分子水平的多项研究表明,PNS具有改善心肌缺血、抗血栓、抗炎、抗氧化、抗动脉粥样硬化、调节血管内皮细胞功能、改善脑缺血损伤等方面的作用。另一方面,通过对PNS以及单体皂苷溶血反应的结构特点、剂量以及检测方法等方面进行文献综述,发现PNS的溶血作用与其分子结构类型及用药剂量密切相关。三七皂苷的结构不同,溶血活性不同,构效关系提示,原人参二醇C-20上糖侧链的长度以及C-3上二糖的连接方式可能影响皂苷的溶血活性。PNS在一定剂量范围内(2.5~250 mg·L-1)无溶血活性,在高浓度(≥500 mg·L-1)时表现出溶血特性。因此,根据三七皂苷单体溶血与抗溶血作用的特点及量效关系,通过控制PNS中具有溶血活性的皂苷单体比例,并通过改进溶血检测方法,可以为三七皂苷制剂临床合理应用提供保障。     

Investigation of plant extracts in traditional medicine of the Brazilian Cerrado against protozoans and yeasts

Aim of the study

To investigate the activities of the 217 plant extracts in traditional medicine of the Brazilian Cerrado against protozoans and yeasts.

Materials and methods

Plant extracts were prepared by the method of maceration using solvents of different polarities. The growth inhibition of chloroquine-resistant Plasmodium falciparum strain (FcB1) was determined by measuring the radioactivity of the tritiated hypoxanthine incorporated. Activity against Leishmania (Leishmania) chagasi and Trypanosoma cruzi was measured by the MTT colorimetric assay. The antifungal tests were carried out by using the CLSI method. The active extracts were tested also by cytotoxicity assay using NIH-3T3 cells of mammalian fibroblasts.

Results

Two hundred and seventeen extracts of plants were tested against Plasmodium falciparum. The eleven active extracts, belonging to eight plant species were evaluated against L. (L.) chagasi, Trypanosoma cruzi, yeasts and in NIH-3T3 cells. The results found in these biological models are consistent with the ethnopharmacological data of these plants. The ethyl acetate extract of Diospyros hispida root showed IC₅₀ values of 1 μg/mL against Plasmodium falciparum. This extract demonstrated no toxicity against mammalian cells, resulting in a significant selectivity index (SI) of 435.8. The dichloromethane extract of Calophyllum brasiliense root wood was active against Cryptococcus gattii LMGO 01 with MIC of 1.95 μg/mL; and Candida albicans ATCC 10231 and Candida krusei LMGO 174, both with MIC of 7.81 μg/mL. The same extract was also active against Plasmodium falciparum and L. (L.) chagasi with IC₅₀ of 6.7 and 27.6 μg/mL respectively. The ethyl acetate extract of Spiranthera odoratissima leaves was active against Cryptococcus gattii LMGO 01 with MIC of 31.25 μg/mL, and against Plasmodium falciparum with IC₅₀ of 9.2 μg/mL and Trypanosoma cruzi with IC₅₀ of 56.3 μg/mL.

Conclusion

The active extracts for protozoans and human pathogenic yeasts are considered promising to continue the search for the identification and development of leading compounds.     

Effect of feeding Mucuna pruriens on helminth parasite infestation in lambs

Ethnopharmacological relevance

Mucuna pruriens is a tropical legume anecdotally reputed to have anthelmintic properties. This study was conducted to examine the validity of such claims.

Aim of the study

The aim of this study was to determine if ingestion of Mucuna seeds reduces helminth parasite infestation in lambs.

Materials and methods

Thirty-six Dorper × Katahdin ram lambs were assigned to three treatments, a cottonseed meal based control diet, a diet in which Mucuna replaced cottonseed meal and the control diet with levamisole (7.5 mg/kg body weight) administration. All diets were isonitrogenous and isocaloric. The 12 lambs in each treatment were assigned randomly to 4 pens, each containing 3 lambs. Lambs were trickle infected three times per week by gavage with infectious Haemonchus contortus larvae (2000 larvae/lamb) for 3 weeks.

Results

Levamisole treatment decreased fecal egg counts by 87% and abomasal worm counts by 83%. Mucuna intake did not statistically affect fecal egg counts or abomasal worm counts, though numerical (P > 0.10) reductions of 7.4% and 18.1%, respectively were evident. Anemia indicators, feed intake, and lamb growth were unaffected by treatment.

Conclusions

Levamisole reduced the Haemonchus parasite burden in lambs significantly but feeding Mucuna reduced the burden by levels unlikely to eliminate the clinical effects of parasitism.     

中国石斛属植物文献计量研究

石斛是珍稀濒危中药材,目前正处于快速发展阶段。为全面了解我国石斛属植物研究的历史和发展现状,作者以1954～2010年"中国知网中国学术期刊网络出版总库"收录的石斛研究文献为依据,采用文献计量学的原理和方法,对我国石斛属植物研究文献从文献年代分布、期刊分布与被引频率、主题分布、研究对象分布、作者与研究机构分布等方面进行了统计与分析。结果表明,我国石斛研究明显分为起步(2个)、停滞、平稳发展、快速上升5个阶段;期刊分布存在离散性与集中性并存的现象,已形成核心期刊研究群,并以《中国中药杂志》、《中草药》和《陕西中医》为代表;研究主题广泛涉及临床与药理、组织培养与种苗繁育、成分分析等多个领域,已经形成比较稳定的研究机构和团队,但研究对象差异显著,以铁皮石斛、金钗石斛和霍山石斛最为集中。我国石斛属植物的研究已取得显著成果,但种植产业发展缓慢,供需矛盾突出,预计种苗繁育与人工种植、产品开发、化学与药理等方面是未来的研究热点,其文献报道仍将进一步上升。     

Screening of the topical anti-inflammatory activity of the bark of Acacia cornigera Willdenow, Byrsonima crassifolia Kunth, Sweetia panamensis Yakovlev and the leaves of Sphagneticola trilobata Hitchcock

Ethnopharmacological relevance

An investigation of topical anti-inflammatory activity was undertaken on plants used in Central America traditional medicine.

Aim of study

Four herbal drugs used in the folk medicine of Central America to treat inflammatory skin affections (Acacia cornigera bark, Byrsonima crassifolia bark, Sphagneticola trilobata leaves and Sweetia panamensis bark) were evaluated for their topical anti-inflammatory activity.

Materials and methods

Petroleum ether, chloroform and methanol extracts were obtained for herbal medicines and then extracts were tested on Croton oil-induced ear dermatitis model in mice.

Results

Almost all the extracts reduced the Croton oil-induced ear dermatitis in mice and the chloroform ones showed the highest activity, with ID₅₀ (dose giving 50% oedema inhibition) values ranging from 112 μg/cm² (Byrsonima crassifolia) to 183 μg/cm² (Sphagneticola trilobata). As reference, ID₅₀ of the non-steroidal anti-inflammatory drug indomethacin was 93 μg/cm².

Conclusions

Lipophilic extracts from these species can be regarded as potential sources of anti-inflammatory principles.     

厚朴与凹叶厚朴群体遗传学研究

目的:对厚朴与凹叶厚朴的群体遗传学进行研究,为中药厚朴的质量控制提供分子生药学方面的依据。方法:对厚朴与凹叶厚朴15个居群应用2个叶绿体基因间序列psbA-trnH和trnL-trnF进行PCR扩增并测序,计算厚朴与凹叶厚朴单倍型频率,用程序HaploNst分析遗传多样性和遗传结构,应用TCS version 1.13软件构建单倍型网状进化树。结果:厚朴与凹叶厚朴均无特有单倍型存在,但单倍型频率存在显著差异,已开始出现遗传分化的趋势,NST略大于GST。结论:厚朴与凹叶厚朴在遗传上已出现遗传分化的趋势,但尚未完全分化成彼此独立的单系。     

Antifungal activity of Brazilian medicinal plants involved in popular treatment of mycoses

A survey of medicinal plants used to treat common mycoses was done in the Curituba district, Sergipe State, Brazil. One hundred inhabitants were interviewed by health agents and traditional healers. Four different plants were the most cited (more than 50% of the citations): Ziziphus joazeiro, Caesalpinia pyramidalis, Bumelia sartorum and Hymenea courbaril. The aqueous extracts obtained following traditional methods and using different parts of these plants, were submitted to drop agar diffusion tests for primary antimicrobial screening. Only the water infusion extract of Ziziphus joazeiro and Caesalpinea pyramidalis presented a significant antifungal activity against Trichophyton rubrum, Candida guilliermondii, Candida albicans, Cryptococcus neoformans and Fonsecaea pedrosoi, when compared to the antifungal agent amphotericin B. The minimal inhibitory concentration (MIC) of the bioactive extracts was evaluated by the microdilution method. Best activity with a MIC of 6.5 microg/ml for both extracts was observed against Trichophyton rubrum and Candida guilliermondii. Ziziphus joazeiro and Caesalpinea pyramidalis extracts presented also low acute toxicity in murine models. The present study validates the folk use of these plant extracts and indicates that they can be effective potential candidates for the development of new strategies to treat fungal infections.     

常用藏药“蒂达”(藏茵陈)基原物种药用合理性及资源利用价值评价

目的:对“蒂达”各品种不同基原植物药用合理性及其资源利用价值作出评价.方法:采用HPLC指纹图谱共有特征峰、结合SPSS聚类分析方法,评价“蒂达”不同品种基原植物的成分组成相似性.结果:“蒂达”不同品种涉及的来源有龙胆科獐牙菜属、花锚属、扁蕾属及肋柱花属共15种1变种33个样品,指纹图谱全谱相似度较差;以10个共有峰成分的相对面积聚类,其基原植物可分别聚为4个组;上述4个属的属间成分组成差异较大;其中獐牙菜属不同种间成分组成变化较大,而其他各属种间成分组成相似度较高.结论:上述龙胆科4属植物均含有具有保肝利胆生物活性的环烯醚萜类、(山)酮类及三萜类成分,藏医学将其归为“蒂达”一类药材使用具有一定的物质基础;鉴于“蒂达”类药材不同基原各品种间在成分组成上差异较大,分别制定各品种的HPLC指纹图谱,是鉴别和控制各品种质量的有效手段,可有效控制各基原品种质量.     

Experimental study on anti-inflammatory activity of a TCM recipe consisting of the supercritical fluid CO2 extract of Chrysanthemum indicum, Patchouli Oil and Zedoary Turmeric Oil in vivo

Ethnopharmacological relevance

Chrysanthemum indicum (Compositae) Linné, Pogostemon cablin (Blanco) Benth and Curcuma wenyujin (Zingiberaceae) Y. H. Chen et C. Ling are three of the extensively used herbal remedies among traditional Chinese medicines for the purpose of anti-inflammation. A traditional Chinese medicine (TCM) recipe named CPZ consisting extracts of the above three herbs, has shown noteworthy anti-influenza activity, which is closely related to its anti-inflammatory feature.

Aim of this study

To investigated the anti-inflammtory activity of CPZ in vivo for a further exploration of the recipe's anti-inflammatory properties.

Materials and methods

The anti-inflammatory property of CPZ on acute inflammation was evaluated by inflammatory models of dimethylbenzene (DMB)-induced ear vasodilatation and acetic acid-induced capillary permeability enhancement in mice, as well as the carrageenan-induced paw edema rat model, in which inflammation-related cytokine including prostaglandin E₂ (PGE₂), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and nitric oxide (NO) in the edematous paw tissue were determined by enzyme linked immunosorbent assay (ELISA). Moreover, effect of CPZ on chronic inflammation was observed through granuloma formation in rats subjected to cotton pellet implantation.

Results

CPZ (340, 170, and 85 mg/kg for mice, p.o.) not only decreased the DMB-induced ear vasodilatation but also attenuated capillary permeability under acetic acid challenge in mice. And the significant inhibition on carrageenan-induced paw edema was observed. Further more, the ELISA results showed that CPZ (170, 85, and 42.5 mg/kg for rats, p.o.) could up-regulate the level of IL-1β in the edema paw tissue of rats significantly while down-regulate that of PGE₂, but no apparent effect on TNF-α or NO was observed in the test. Besides, CPZ had a certain degree of restraining effect on the cotton pellet-induced granuloma formation in rats and the highest dose of 170 mg/kg even showed a significant suppression on it.

Conclusion

The above results indicated that CPZ possessed a potent anti-inflammatory activity, which is indicated to be closely associated with its regulation on IL-1β and PGE₂ thereby mediating the inflammatory response acting at an appropriate level.     

虫草属EST-SSR标记系统的建立研究

目的:通过球孢虫草、蛹虫草EST设计EST-SSR引物,建立虫草属EST-SSR标记系统.方法:从NCBI公共数据库下载获得虫草EST,利用Sequece Seiners 1.2软件去除冗余序列并设计引物,进行PAGE电泳.结果:通过去除EST总序列中低质量的和冗余的序列后,得到全长为2 953 173 bp的4 556条无冗余球孢虫草EST.从中发掘出718个EST-SSR,分布于616条EST中,出现频率是15.8％.平均分布频率是每4 096 bp出现1个,三核苷酸重复序列有419个,是出现最多的重复类型.蛹虫草EST去冗余后得到1 363条无冗余EST,共含有1 117个EST-SSR,出现频率为81.95％,出现最多的重复类型是A核苷酸重复.根据球孢虫草EST-SSR序列,设计合成50对引物,有扩增产物的引物为34对,占总设计引物数的68％.根据蛹虫草EST-SSR,设计合成40对引物,有扩增产物的引物为39对,占总设计引物数的97.5％.基于SSR标记进行聚类分析,7种虫草无性型均能分开,且分为4支.结论:虫草属EST-SSR出现频率较高、类型较丰富、多态性潜能较高,具有较高的利用价值.球孢虫草和蛹虫草EST开发的SSR标记在虫草属有较好的转移性与通用性,可以很好的应用于虫草种间遗传关系的研究.应用虫草物种EST建立分子标记是一条简便而又有效的途径.     

Regulation effects on abnormal glucose and lipid metabolism of TZQ-F,a new kind of Traditional Chinese Medicine

Aim of the study

Based on the recipe of the traditional anti-diabetic formula TZQ, we developed TZQ-F, a new formula including 8 fractions isolated from Red Paeony root, Mulberry leaf, Lotus leaf, Danshen root and Hawthorn leaf with a good quality assurance. The study was aimed at fraction preparation and effects of the fractions on abnormal glucose and lipid metabolism.

Materials and methods

The active fractions were obtained by macroporous resin, ion-exchange resin and polyamide resin column chromatographies. HPLC analyses were used for quality control. In vitro mechanism study included DPPH radical scavenging, AGEs formation inhibition, α-glucosidase inhibition and lipase inhibition, and rats on high-fat diet were used for in vivo study.

Results

In vitro mechanism study showed that among the 8 fractions, three of them had inhibition effects on intestinal disaccharase, three with inhibition effects on lipase, and five with effects of free radical scavenging. In vivo study showed that after 4 weeks of treatment, TZQ-F significantly decreased the levels of serum total cholesterol, TG, glucose, LDL-C and HDL-C in rats on high-fat diet. Consistent with the in vitro and in vivo results, histology study demonstrated that TZQ-F alleviated hepatic steatosis induced by high-fat diet.

Conclusions

TZQ-F possesses the potential regulation effects on abnormal glucose and lipid metabolism.