双眼先天性无虹膜一例

患儿,男,10岁.自幼双眼视力差,畏光.于2008年3月15日就诊.患儿为足月顺产,父母非近亲结婚,家族中无遗传病史.全身检查:一般情况好,智力及发育无异常.眼部检查:视力右眼0.3,矫正0.8(-3.75DS/ 2.50DC×170°);左眼0.2,矫正0.7(-4.25DS/ 2.75DC×10°);双眼眼位正常,各方向运动自如.     

Infectious keratitis in Vietnam: etiology, organisms, and management at Vietnam National Eye Hospital

AIM:To report the etiologies,risk factors,treatments,and outcomes of infectious keratitis(IK)at a major Vietnamese eye hospital.METHODS:This is a retrospective review of all cases of IK at Vietnam National Eye Hospital(VNEH)in Hanoi,Vietnam.Medical histories,demographics,clinical features,microbiological results,and treatment outcomes were reviewed.RESULTS:IK was diagnosed in 1974 eyes of 1952 patients,with ocular trauma being the greatest risk factor for IK(34.2%),frequently resulting from an agriculturerelated injur y(53.3%).The mean duration between symptom onset and presentation to VNEH was 19.3±14.4 d,and 98.7%of patients had been treated with topical antibiotic and/or antifungal agents prior to evaluation at VNEH.Based on smear results of 1706 samples,the most common organisms identified were bacteria(n=1107,64.9%)and fungi(n=1092,64.0%),with identification of both bacteria and fungi in 614(36.0%)eyes.Fifty-five of 374 bacterial cultures(14.7%)and 426 of 838 fungal cultures(50.8%)were positive,with the most commonly cultured pathogens being Pseudomonas aeruginosa,Streptococcus pneumonia,Fusarium spp.,and Aspergillus spp.Corneal perforation and descemetocele developed in 391(19.8%)and 93(4.7%)eyes,respectively.Medical treatment was successful in resolving IK in 50.4%eyes,while 337(17.1%)eyes underwent penetrating or anterior lamellar keratoplasty.Evisceration was performed in 7.1%of eyes,most commonly in the setting of fungal keratitis.CONCLUSION:Ocular trauma is a major risk factor for IK in Vietnam,which is diagnosed in almost 400 patients each year at VNEH.Given this,and as approximately one quarter of the eyes that develop IK require corneal transplantation or evisceration,greater emphasis should be placed on the development of prevention and treatment programs for IK in Vietnam.     

医源性眼巩膜双穿孔伤一例

1 病例 患者女,汉族,63岁.右眼视物不清3年确诊老年性白内障于2006年8月5日人院,检查:全身检查无异常;右眼视力光感,光定位和色觉正常;前房正常深浅,瞳孔3 mm,对光反射存在,晶状体乳白色浑浊,眼压15.55 mmHg(1 mmHg=0.133 kPa).     

Prevalence and risk factors of dry eye disease in young and middle-aged office employee:a Xi’an Study

AIM:To estimate the prevalence of and risk factors for dry eye disease(DED)in young and middle-aged office employee in Xi’an.METHODS:This cross-sectional study of the prevalence of and risk factors for DED investigated 486 young and middle-aged Chinese office employee in Xi’an.DED symptoms and potential risk factors were assessed using the ocular surface disease index combined with a risk factors questionnaire,and tear function was evaluated using the tear film break-up time and Schirmer’s test.Possible risk factors for DED were estimated by binary Logistic regression analysis.RESULTS:DED was diagnosed in 100 females and 96 males,giving a prevalence of 40.3%[95%confidence interval(CI)=36.0%-44.7%].The multivariate binary Logistic regression model indicated that the possible risk factors for DED were being female(OR=1.592,95%CI=1.034-2.451,P=0.035),being aged≥40 y(OR=1.593,95%CI=1.034-2.454,P=0.035),using a VDT daily for>6 h(OR=1.990,95%CI=1.334-2.971,P=0.001),the presence of central air conditioning(OR=1.548,95%CI=1.053-2.276,P=0.026),and self-reported dryness of the mouth and nose(OR=1.589,95%CI=1.071-2.357,P=0.021).CONCLUSION:There is a high prevalence of clinically diagnosed DED in young and middle-aged video displayterminal(VDT)users.Interventions against the modifiable risk factors should be taken to prevent the occurrence and development of DED in this population.     

Spasm of the near reflex:a common diagnostic dilemma?

AIM:To report the clinical characteristics and diagnostic procedures used in patients with spasm of the near reflex(SNR),in order to present common investigation strategies and diagnostic pitfalls.METHODS:Retrospective case series of twenty-two patients,mainly children,with SNR or accommodation spasm(AS).AS was diagnosed on the basis of blurred vision and a difference of>2 dioptres between manifest and cycloplegic retinoscopy.If esotropia and miosis were present,the patients were diagnosed with SNR.All patients underwent visual acuity testing,orthoptic evaluation,assessment of refraction before and after cycloplegia,and dilated fundoscopy.Additional diagnostic investigations,such as neuroimaging,lumbar puncture(LP),electrophysiology and blood tests,were also recorded.Screen use among children was assessed in hours per day.RESULTS:There were 19 female and 3 male patients(age range 7-33 y,median=10 y).Seventeen patients had AS and 5 patients had SNR,with episodic blurry vision and headaches being the most common symptoms.Brain neuroimaging was performed in six patients(27%),although only one had a history of brain trauma.Two of those patients underwent visual evoked potentials and three also underwent LP and received intravenous steroid therapy.The majority of patients(90%)reported prolonged daily screen time(>2 h/d),and in 55%of cases there were concurrent social problems or psychological triggers.Treatment consisted of careful explanation of the condition,atropine1%eye drops and full cycloplegic correction by means of bifocal glasses.CONCLUSION:The diagnosis of SNR and AS may be challenging,because symptoms are usually intermittent and nonspecific,and a large number of patients are often subjected to redundant and potentially time-consuming examinations and treatment,that may exaggerate the underlying psychological disorder.Hence,detailed clinical testing and assessment of psychosocial profile is necessary,in order to avoid unnecessary investigations.Neuroimaging should be performed only in selected cases.Finally,due to prolonged screen use SNR and AS may become more frequent in the future.     

Corneal stromal mesenchymal stem cells: reconstructing a bioactive cornea and repairing the corneal limbus and stromal microenvironment

Corneal stroma-derived mesenchymal stem cells(CS-MSCs) are mainly distributed in the anterior part of the corneal stroma near the corneal limbal stem cells(LSCs). CS-MSCs are stem cells with self-renewal and multidirectional differentiation potential. A large amount of data confirmed that CS-MSCs can be induced to differentiate into functional keratocytes in vitro, which is the motive force for maintaining corneal transparency and producing a normal corneal stroma. CS-MSCs are also an important component of the limbal microenvironment. Furthermore, they are of great significance in the reconstruction of ocular surface tissue and tissue engineering for active biocornea construction. In this paper, the localization and biological characteristics of CS-MSCs, the use of CS-MSCs to reconstruct a tissue-engineered active biocornea, and the repair of the limbal and matrix microenvironment by CS-MSCs are reviewed, and their application prospects are discussed.     

Incidence of endophthalmitis after phacoemulsification cataract surgery: a Meta-analysis

AIM:To evaluate the overall endophthalmitis incidence and the effectiveness of potential prophylaxis measures following phacoemulsification cataract surgery(PCS).METHODS:The Pub Med and Web of Science databases were searched from inception to April 30^th,2021.We included studies that reported on the incidence of endophthalmitis following PCS.The quality of the included studies was critically evaluated with the Newcastle-Ottawa quality assessment scale.The random effect or the fixed-effects model was used to evaluated the pooled incidence based on the heterogeneity.The publication bias was assessed by Egger’s linear regression and Begg’s rank correlation tests.RESULTS:A total of 39 studies containing 5 878 114 eyes were included and critically appraised in the Meta-analysis.For overall incidence of endophthalmitis after PCS,the Meta-analysis yielded a pooled estimate of 0.092%(95%CI:0.083%-0.101%).The incidence appeared to decrease with time(before 2000:0.097%,95%CI:0.060%-0.135%;2000 to 2010:0.089%,95%CI:0.076%-0.101%;after 2010:0.063%,95%CI:0.050%-0.077%).Compared with typical povidone-iodine solution(0.178%,95%CI:0.071%-0.285%) and antibiotics subconjunctival injections(0.047%,95%CI:0.001%-0.095%),the use of intracameral antibiotics significantly reduced the incidence of endophthalmitis after PCS(0.045%,95%CI:0.034%-0.055%,RR:7.942,95%CI:4.510-13.985).CONCLUSION:Due to the advancement of phacoemulsification technology and the widespread use of intracameral antibiotics,the incidence of endophthalmitis following PCS shows a decreasing trend over time.The use of intracameral antibiotics administration will significantly reduce the risk of endophthalmitis.     

Preliminary studies of constructing a tissue-engineered lamellar corneal graft by culturing mesenchymal stem cells onto decellularized corneal matrix

AIM:To construct a competent corneal lamellar substitute in order to alleviate the shortage of human corneal donor.METHODS:Rabbit mesenchymal stem cells(MSCs)were isolated from bone marrow and identified by flow cytometric,osteogenic and adipogenic induction.Xenogenic decellularized corneal matrix(XDCM)was generated from dog corneas.MSCs were seeded and cultured on XDCM to construct the tissueengineered cornea.Post-transplantation biocompatibility of engineered corneal graft were tested by animal experiment.Rabbits were divided into two groups then underwent lamellar keratoplasty(LK)with different corneal grafts:1)XDCM group(n=5):XDCM;2)XDCM-MSCs groups(n=4):tissue-engineered cornea made up with XDCM and MSCs.The ocular surface recovery procedure was observed while corneal transparency,neovascularization and epithelium defection were measured and compared.In vivo on focal exam was performed 3 mo postoperatively.RESULTS:Rabbit MSCs were isolated and identified.Flow cytometry demonstrated isolated cells were CD90 positive and CD34,CD45 negative.Osteogenic and adipogenic induction verified their multipotent abilities.MSC-XDCM grafts were constructed and observed.In vivo transplantation showed the neovascularization in XDCMMSC group was much less than that in XDCM group postoperatively.Post-transplant 3-month confocal test showed less nerve regeneration and bigger cell-absent area in XDCM-MSC group.CONCLUSION:This study present a novel corneal tissue-engineered graft that could reduce post-operatively neovascularization and remain transparency,meanwhile shows that co-transplantation of MSCs may help increase corneal transplantation successful rate and enlarge the source range of corneal substitute to overcome cornea donor shortage.     

Comparison of correcting myopia and astigmatism with SMILE or FS-LASIK and postoperative higher-order aberrations

AIM:To compare the effect of myopia and astigmatism correction and postoperative change in higher-order aberration as results of receiving small-incision lenticule extraction(SMILE)and femtosecond laser-assisted in situ keratomileusis(FS-LASIK).METHODS:A prospective and non-randomized controlled study was conducted.The subjects are divided into two groups according to different operations received:229 eyes of 116 patients in the SMILE group and 168 eyes of 86 patients in the FS-LASIK group.All subjects were followed up for 3 mo by monitoring their uncorrected visual acuity(UCVA),best-corrected visual acuity(BCVA),spherical equivalent,higher-order aberrations,and the preoperative and postoperative complications.RESULTS:At 1 wk,1,and 3 mo post-surgery,224 eyes(97.8%),227 eyes(99.1%)and 229 eyes(100%)had UCVA≥20/20 in the SMILE group,while 165 eyes(98.2%),167 eyes(99.4%)and 167 eyes(99.4%)had UCVA≥20/20 in the FS-LASIK group,respectively(χ²=0.146,2.135,and 1.124;all P>0.05).BCVA reduction was not observed in both groups at 1 and 3 mo of post-surgery(χ²=0.734 and 1.898,P>0.05).There was no statistically significant difference in the spherical equivalent between the two groups at 1 and 3 mo post-surgery,though the percentage of the spherical equivalent within±0.50 D at 3 mo postsurgery was 98%in the SMILE group,which was higherthan that of the FS-LASIK group(92%,χ²=1.872,P>0.05).The root mean square(RMS)values of total high-order aberration,coma,and spherical aberration of the two groups increased significantly in the early postoperative period and decreased after 3 mo,but the values were still higher than the preoperative levels(P<0.05);there was no significant difference between the two groups in the RMS values of total higher-order aberrations and specific higherorder aberrations(P>0.05).The incidence of complications in the SMILE group was lower than that in the FS-LASIK group(χ²=14.52,P<0.05).CONCLUSION:SMILE and FS-LASIK can effectively treat myopia,significantly improve visual acuity,and increase the total high-order aberration,spherical aberration,and coma.The incidence of complications after SMILE is relatively low.     

眶内巨大鼻窦囊肿手术治疗三例报告

1 病例 例1:患者男,38岁.因左眼眶内发现肿物3年多并进行性增大,经多家医院诊治未愈于1998年4月6日来本院.患者一般情况好,全身检查未见特殊.左眼眶内侧缘高高隆起约35 mm.触及一表面光滑、无压痛、不活动、与皮肤不粘连、有波动感的肿块,大小约40 mm×40 mm.眼球被严重挤压向外侧眶下缘突出约30 mm.双眼视力1.5,无复视.右眼检查正常.     

医院、幼儿园联合治疗弱视58例分析

目的 研究医院与幼儿园联合治疗弱视的可行性.方法 对58例(84眼)弱视患儿采用在配戴合适度数眼镜基础上进行弱视综合训练,治疗方案由眼科医师制定,具体训练在幼儿园中进行.结果 经过两年治疗,弱视患儿基本痊愈率84.5%.结论 由医院与幼儿园联合治疗弱视的模式值得推广.     

实验动物的眼压测量

青光眼是危害视功能,引起失明的重要原因之一.对青光眼病因、病理、预防、诊断、治疗的研究需要合适的青光眼动物模型.眼压是影响青光眼发生、发展、预后的重要因素,能准确测量实验动物的眼压对研究青光眼的发生机制、病理变化、治疗方法以及预后都有重大意义.本文就实验动物眼压测量的方法进行综述.     

陕西省农村50岁以上人群白内障和盲的患病率调查

目的:调查陕西省农村50岁以上老年人群中白内障的患病率,并评价由白内障造成视力损伤的情况.方法:2003-07/12,采用整体随机分层抽样法在陕西省洋县、靖边县和富平县调查8 500人,其中≥50岁人群2 213人.所有受检人员均进行标准问卷调查及详细的眼科检查.视力检查使用LogMAR视力表分别对每只眼进行测量,晶状体混浊使用国内标准在裂隙灯下进行评价.结果:1 775人接受了检查,应答率为80.2%,50岁以上人群白障患病率为37.2%,男性白内障患病率为28.6%,女性白障患病率为44.3%,男性患病率低于女性(P＜0.01),50岁人群白内障患病率为17.9%,70岁以上人群则上升到82.4%,白内障的患病率随着年龄增长而明显上升(P＜0.01).其中皮质性白内障患病率为34.5%,核性白内障为9.2%,后囊下白内障为6.7%.在1 775名受检者中,20人在检查前实施了白内障手术.该人群中双眼盲和双眼低视力的患病率分别为2.1%和3.2%,由白内障造成的双眼盲、单眼盲、双眼低视力、单眼低视力分别占56.8%,68.8%,38.6%,73.8%.结论:调查结果表明白内障在陕西省农村还是一个比较严重的公众健康问题.白内障手术在农村地区实施的情况还很低.白内障是造成视力损伤的最主要原因.     

Multimodal therapy in the management of primary orbital mesenchymal chondrosarcoma

AIM:To evaluate the ophthalmic manifestations,radiographic features,and prognosis of Chinese patients with primary orbital mesenchymal chondrosarcoma(MCS).METHODS:The study included 6 cases with primary orbital MCS treated at Tianjin Eye Hospital from January 2009 to December 2019.Patitent ophthalmic manifestations,radiographic features,diagnosis,pathology,therapeutic regimens,and prognosis were retrospectively reviewed.RESULTS:Six patitents with primary orbital MCS were identified.The mean age at the first visit was 33y(range,25-42y).All six patients displayed manifestations of exophthalmos,diplopia,limitation of eye displacement,upper eyelid oedema,decreased visual acuity and ptosis.The mean disease history and range were 5 and 2-8mo,respectively.The tumors were located in the superonasal extraconal compartment(2/6,33.3%),intraconal compartment(2/6,33.3%),and bitemporal extraconal compartment(2/6,33.3%),respectively.Radiographic features were a well-defined,orbital mass with calcification and ossification on computed tomography(CT),and marked heterogenous enhancement on dynamic magnetic resonance imaging(MRI).Five patients were treated with tumor resection and one patient received orbital exenteration.Five patients in the cohort received postoperative radiation therapy,two patients received chemotherapy,and one patient did not receive postoperative adjuvant therapy because he refused.The histopathologic classification revealed a tumour composed of a mixture of mature chondroid tissue surrounded by small,round,and undifferentiated mesenchymal cells.Immunohistochemistry revealed Bcl-2,vimetin,CD99,and S-100 were expressed were expressed.After surgeries,two patients have developed a local recurrence.The median recurrence time of 58 mo(52-64 mo).One patient had distant recurrence included the lungs occurred 52mo after the initial surgery.CONCLUSION:The possibilty of orbital MCS need to be considered when a painless,slowly growing orbital mass with calcification and ossification.From our experience,trimodality treatment of radiation therapy,chemotherapy and surgery maybe the best option.Orbital MCS has a high tendency for late recurrence,regular long-term follow-up after complete excision is mandatory.     

Cost-effectiveness analysis of tele-retinopathy of prematurity screening in Iran

AIM:To conduct a cost-utility analysis of the teleretinopathy of prematurity(ROP)screening program against no screening.METHODS:A decision tree model was developed to identify and treat the infants with threshold ROP through the tele-screening system compared with no screening program from the societal perspective.We used the quality adjusted life years(QALY)index to measure the scenarios’effectiveness,which was discounted for the future years by 0.058.One hundred twenty-six randomly selected newborns with ROP required treatment were investigated to extract the treatment information.We considered the direct medical and non-medical costs in cost calculations analysed by the bottom-up approach.The figures of the model’s inputs were calculated using the Monte Carlo simulation that generated 1000 random iteration of the data,and a one-way sensitivity analysis was performed on the model to cope with the potential uncertainties.RESULTS:The total and per capita needed the budget to establish a tele-ROP screening system were estimated at over 1.5 million and 35.13 USD,respectively.The total cost of identifying and treating an ROP case in tele-screening and no screening strategies were obtained as 108.72 and 63.52 USD,respectively,and their lifetime discounted QALY gained were calculated as 15.39 and 15.11,respectively.Therefore,incremental cost-effectiveness ratio(ICER)of tele-screening strategy against the competitive strategy was achieved as 161.43 USD.CONCLUSION:Tele-ROP screening program is one of the most cost-effective interventions in the Iranian health system and has a high priority to receive a budget for implementation.     

环孢素A壳聚糖纳米微粒预防兔小梁切除术后滤过道瘢痕化的研究

目的 研究自制的环孢素A壳聚糖纳米微粒对实验性兔小梁切除术后滤过道瘢痕化的预防作用.方法 48只健康大耳白兔随机分为4组,均行单眼小梁切除术.术毕球结膜下分别注射CS(CsA)-NP、环孢素A(CsA)、壳聚糖纳米微粒(CS-NP)各0.5mL,对照组结膜下注射平衡盐溶液(BSS)0.5mL.于术后1d,1、2、4、8、12周查眼压(IOP)、滤过泡、角膜、虹膜、前房、晶状体、视网膜.结果 术后8周内各组平均IOP与术前比较差异均有统计学意义(P＜0.05).术后第4周CS(CsA)-NP组与CsA组平均IOP差异有统计学意义(F=2.91,P＜0.05).4组功能性滤过泡的生存时间差异有统计学意义(χ2=15.51,P＜0.01).结论 CS(CsA)-NP能抑制滤过区成纤维细胞的增生,有助于功能性滤过泡的维持,有效控制术后IOP,与CsA相比疗效高、不良反应小.     

增龄大鼠晶状体老化过程中脂质过氧化及抗氧化能力变化

目的 了解大鼠晶状体脂质过氧化及抗氧化能力随年龄增长的变化趋势.方法 雄性SD大鼠60只,按月龄不同分为1月龄组、3月龄组、6月龄组、12月龄组、18月龄组、24月龄组共6组,每组10只;处死动物取出眼球,采用硫代巴比妥酸比色法与化学发光法检测各组大鼠晶状体组织中谷胱甘肽(glutathione hormone,GSH)、超氧化物岐化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)含量.结果 大鼠晶状体GSH含量1月龄组、3月龄组、6月龄组、12月龄组、18月龄组、24月龄组分别为(30.49±7.39)mg、(33.32±6.13)mg、(29.04±3.06)mg、(28.65±4.84)mg、(22.03±4.24)mg、(20.53±3.22) mg;各增龄组GSH含量经F检验P＜0.01,GSH含量与月龄负相关(P＜0.01);大鼠晶状体SOD含量1月龄组、3月龄组、6月龄组、12月龄组、18月龄组、24月龄组分别为(24.63±1.25)U、(25.39±1.64)U、(23.17±1.81)U、(23.24±3.11)U、(20.47±1.08)U、(19.11±2.97) U;各增龄组SOD含量经F检验P＜0.01,SOD含量与月龄负相关(P＜0.01).大鼠晶状体MDA含量1月龄组、3月龄组、6月龄组、12月龄组、18月龄组、24月龄组分别为(49.63±4.38)nmol、(52.19±3.82)nmol、(53.09±6.45)nmol、(53.59±4.66)nmol、(59.80±4.23)nmol、(62.21±3.93)nmol;各增龄组MDA含量F检验P＜0.01,MDA含量与月龄正相关(P＜0.01).结论 大鼠晶状体GSH、SOD及MDA随年龄增长分别呈降低、降低、升高趋势,提示晶状体老化过程伴随晶状体脂质过氧化损伤及抗氧化能力下降.     

以猪角膜脱细胞基质为载体培养猫骨髓内皮祖细胞构建角膜后板层的实验研究

目的 探讨以猪角膜脱细胞基质(cornea acellular matrix,CACM)为载体培养猫骨髓内皮祖细胞(endothelial progenitor cells,EPC)体外构建角膜后板层的可行性.方法 分离培养猫骨髓EPC,并用PKH26标记,观察标记效率.用10 g·L-1 Triton X-100脱细胞,联合逐步切薄角膜、超净台中自然晾干法制备角膜脱细胞基质,行HE染色,Hoechst染核,电镜观察猪CACM的胶原排列和细胞脱除情况.将标记的EPC接种到猪CACM上,观察细胞的生长情况.结果 培养的猫骨髓EPC形态上呈多边形,几乎全部EPC可以标记上PKH26,细胞膜呈现红色荧光,至少可维持1个月.制备的猪CACM可保持整齐的胶原排列,细胞完全脱除.EPC在CACM上呈单层生长,贴附较好.构建的组织工程角膜后板层与正常角膜相似.结论 以猪CACM为载体培养猫骨髓EPC可体外构建组织工程角膜后板层,为进一步体内移植打下基础.     

眼前段分析系统（Pentacam）临床应用的意义

目的 探讨Pentacam综合眼前段三维分析系统在闭角型青光眼的早期筛查和青光眼白内障联合手术疗效量化评价方面的意义.方法 60岁以上志愿者25人（25眼）,为正常对照组,原发性急性闭角型青光眼25例（25眼）和原发性慢性闭角型青光眼25例（25眼）,应用Pentacam综合眼前段三维分析系统测量前房形态参数（前房深度、前房容积、前房角）,并分别与青光眼白内障联合手术前与术后1d、7d、30 d测量的前房形态参数：前房深度、前房容积、前房角进行比较.结果 正常对照组前房形态参数的测量值均大于原发性急、慢性闭角型青光眼两个组,差异均有统计学意义;青光眼两组之间比较,差异无统计学意义.两个青光眼组在青光眼白内障联合手术后各时间点前房深度较术前均加深,前房容积较术前均变大,前房角较术前均增宽,差异均有统计学意义.结论 Pentacam综合眼前段三维分析系统可以用来量化评价青光眼白内障联合手术后的眼前段形态的变化,为预后的评估提供参考.     

Therapeutic potential of Rho-associated kinase inhibitor Y27632 in corneal endothelial dysfunction: an in vitro and in vivo study

AIM:To investigate the effects of a selective inhibitor of Rho-associated kinase(ROCK),Y-27632,on inbred Wuzhishan porcine corneal endothelial cells(PCECs)in vitro and in vivo studies.METHODS:Primary PCECs were trypsinized from Wuzhishan miniature porcine corneal tissues.The optimal concentration of Y-27632 on PCECs was determined through MTT and 5-ethynyl-2'-deoxyuridine(EdU)-labeling assays.Seven New Zealand rabbits were used as a corneal endothelial dysfunction model,and a PCECs suspension supplemented with Y-27632 was injected into the anterior chamber of the rabbits.The progression of rabbit corneal opacity and edema were observed by slit lamp examination.The rabbits were sacrificed,and rabbit globes were enucleated for trypan blue-alizarin red staining,hematoxylineosin staining,and immunofluorescence analysis.RESULTS:Administration of 100μmol/L Y-27632 facilitated PCECs'proliferation obviously.The rabbit corneas injected with PCECs suspension and 100μmol/L Y-27632 were restored to transparency significantly after 14d.CONCLUSION:The 100μmol/L Y-27632 treatment improves PCECs'proliferation significantly.And our results suggest that Y-27632 and PCECs can be used to treat corneal endothelial dysfunction.