泌尿生殖道支原体检测及耐药性分析

目的 了解在相关门诊就诊者解脲脲原体（Uu）和人型支原体（Mh）感染及耐药情况，以指导临床诊断和合理用药。方法应用珠海丽珠公司试剂盒进行支原体培养及药敏试验。结果 320例就诊者中支原体培养阳性145例，阳性率45．3％，其中检出Uu109例，阳性率为34．1％（109／320），高于Mh（6例，1．87％）和Uu与Mh混合感染（30例，9．38％）；Uu药敏结果显示，敏感率较高的药物为强力霉素、美满霉素、交沙霉素和克拉霉素，其次为阿齐霉素、左氟沙星。结论 泌尿生殖道支原体感染主要是Uu，明显高于Mh、Uu和Mh混合感染，Uu对强力霉素、交沙霉素和美满霉素的耐药率最低，对氧氟沙星的耐药率最高。     

海口地区泌尿生殖道支原体感染的耐药趋势

目的了解海口地区泌尿生殖道解脲脲原体（uu）和人型支原体（Mh）的感染情况及其在体外对抗菌药物的耐药趋势。方法采用“支原体分离鉴定计数药敏”试剂盒对临床标本作支原体培养，并对检出的628株支原体作药敏试验进行分析。结果检出628株支原体中Uu471株占75．0％，Mh50株占8．0％，Uu合并Mh阳性107例占17．0％。对美满霉素、强力霉素耐药性最低，耐药率分别为15．1％和17．2％；对罗红霉素和阿奇霉素的耐药率最高分别为51．8％和50．3％。结论支原体感染已成为非淋菌性尿道炎的主要病原菌并有多重耐药株的出现，美满霉素和强力霉素可作为治疗的首选药物。     

1535例女性生殖道解脲脲原体和人型支原体的药敏分析

目的探讨女性生殖道解脲脲原体（Uu）和人型支原体（Mh）感染及耐药情况。方法采用解脲脲原体和人型支原体培养、分离鉴定、计数、药敏试剂盒,对我院妇科门诊疑似非淋菌性尿道炎（NGU）女性患者,进行支原体培养、鉴定及药敏试验。结果3926例疑似非淋球菌性尿道炎患者中,总的支原体检出率为43．8％,其中解脲脲原体检出率为38．1％,人型支原体检出率为1％,混合感染率为4．2％。药敏结果表明解脲脲原体对强力霉素、美满霉素最为敏感,敏感率分别为89％、85.3％;而耐药率最高的分别为壮观霉素（89．5％）、罗红霉素（67．9％）。对于人型支原体,敏感性高的药物分别是美满霉素（79、5％）、强力霉素（71．8％）;而耐药性最高的药物分别是罗红霉素（87．2％）、阿齐霉素（84．6％）。结论解脲脲原体在女性NGU疑似病人中感染率较高,强力霉素、美满霉素可作为首选药物对患者进行治疗。     

男性泌尿道支原体感染与药物耐药性分析

目的：观察男性尿道炎患者支原体（解脲支原体Uu和人型支原体Mh）的阳性率及耐药性，指导临床用药。方法：从男性科门诊患有尿道炎的病人中选择64例仅有支原体感染的病例取材并做体外药物耐药性分析。结果：64例感染菌株Uu阳性52例（81．25％），Mh阳性7例（10．94％）、两者混合感染5例（7．81％）。耐药明显的是氧氟沙星（OFL）、司帕沙星（SPA）、左氧氟沙星（LEV）；阿奇霉素（AZI）、交沙霉素（JOS）、罗红霉素ROX）耐药和中介各占50％；克拉霉素（CLA）高敏和中介几乎各占50％；多西环素（DOX）、美满霉素（MIN）耐药最少。结论：在没有条件进行药敏的情况下多西环素、美满霉素、克拉霉素应作为本地区治疗男性非淋菌性尿道炎、前列腺炎、副睾炎的首选药物。     

妇女泌尿生殖道支原体检测和耐药性分析

目的了解妇女泌尿生殖道支原体的感染及耐药情况．为临床提供用药参考。方法采用一体化试剂盒,对疑似非淋菌性尿道炎（NGU）女性患者宫颈分泌物进行支原体培养、鉴定及药敏试验。结果441例疑似非淋菌性尿道炎患者中,支原体培养阳性率为35．8％（158例）。其中主要为解脲支原体（Uu）感染,占感染者的81．0％;人型支原体（Mh）阳性率为5．1％;Uu和Mh混合感染阳性率为13．9％。药敏试验显示支原体主要对美满霉素（82．2％）、强力霉素（80．4％）等抗生素敏感,对环丙沙星（50．3％）、螺旋霉素（48．6％）、氧氟沙星（42．7％）等抗生素耐药。结论NGU女性患者支原体感染主要为解脲支原体,对环丙沙星、螺旋霉素、氧氟沙星等抗生素耐药性较高,而对美满霉素、强力霉素等抗生素较敏感,并提示该类抗生素可作为治疗NGU支原体感染的首选药物。     

泌尿生殖道支原体感染及耐药性监测分析

目的 了解江门地区泌尿生殖道支原体感染情况及其耐药性。方法 对4935例泌尿生殖道感染患者进行解脲脲原体（Uu）和人型支原体（Mh）培养鉴定，并对阳性标本作12种抗生素药敏试验。结果 共检出支原体阳性1672例，总阳性率为33．88％。其中Uu1514例、Mh47例、Uu合并Mh 111例，阳性检出率分别为30．68％、0．95％、2．25％；男性组Uu、Mh、Uu合并Mh的阳性检出率分别为12．47％、0．97％、1．01％，女性组Uu、Mh、Uu合并Mh的阳性检出率分别为46．28％、0．95％、3．3l％。支原体对司巴沙星、阿岐霉素的耐药率较高，分别为33．2％和52．3％、而对美满霉素、强力霉素耐药率较低，分别为6．1％和7，6％。结论 江门地区支原体感染以Uu为主，女性组Uu及Uu合并Mh的检出率高于男性组，支原体对美满霉素和强力霉素较为敏感。     

解脲脲原体与人型支原体感染检测的临床意义

①目的探讨泌尿生殖道支原体感染现状及对抗茵药物的敏感性,以期对临床合理用药提供依据。②方法应用支原体试剂盒对标本进行培养和药敏试验。③结果2119例标本中检出311例阳性,总检出率14．7％,其中男性检出率11．6％,女性检出率17．8％。解脲脲原体（uu）、人型支原体（Mh）混合型（uu＋Mh）检出率分别为83．O％、1．3％、15．8％。Uu对抗茵药物敏感率≥80％的有强力霉索、美满霉素、交沙霉素、克拉霉素;Mh对强力霉素、美满霉素、交沙霉素100％敏感;Uu＋Mh对抗茵药物敏感率〉80％的有强力霉素、美满霉素、交沙霉素。④结论支原体感染中,以Uu感染为主,女性高于男性;感染可选用克拉霉素、交沙霉素、强力霉素、美满霉素,不宜选用氧氟沙星、左旋氧氟沙星。     

男性非淋菌性尿道炎者支原体感染及耐药性分析

目的了解男性泌尿生殖道非淋菌性尿道炎者（NGU）支原体感染及其对抗菌药物的耐药性状况，指导临床合理使用抗菌药物。方法采用珠海丽珠试剂厂生产的支原体试剂盒进行支原体培养鉴定、计数和药敏试验，进行统计分析。结果从638份男性泌尿生殖道NGU标本中共检出253株支原体，阳性率39．7％，其中解脲脲原体（uu）205株，占81．0％，人型支原体（Mh）8株，占3．2％，UU合并Mh阳性40株（份），占15．8％。检出支原体对美满霉素、强力霉素耐药率最低，均为10．8％，对氧氟沙星、罗红霉素和阿奇霉素的耐药率最高，分别为53．0％、52．6％和50．6％。结论支原体已成为男性NGU的主要病原体并以Uu为主，已有多重耐药株的出现，美满霉素和强力霉素可作为治疗的首选药物。     

非淋菌性尿道炎支原体检测与耐药性分析

目的调查湖南长沙地区来长沙第四医院就诊的男女性非淋菌性尿道炎（NGU）患者支原体感染情况、抗生素的耐药率、流行趋势及年龄分布情况。方法采用珠海丽珠试剂有限公司解脲脲原体（Uu）、人型支原体（Mh）分离鉴定、计数、药敏试剂盒对1160例本地男女性尿道炎患者进行Uu和Mh检测及药敏试验，然后进行统计学分析。结果1160例泌尿生殖道感染患者，支原体培养阳性370例，总阳性率31．9％；其中Uu单独阳性282例（76．2％）；Mh单独阳性12例（3．2％）；Uu、Mh混合感染76例（20．6％）：女性阳性率34．9％；男性阳性率23．3％；女性感染阳性率显著高于男性p〈0．01（x^2=13．700）；年龄分布显示90％以上的患者在20—40岁范围内；且Uu和（或）Mh每年感染增长幅度有逐年下降的趋势；药敏试验结果显示单独Uu感染的临床治疗宜首选强力霉素；单独Mh感染首选交沙霉素；而Uu和Mh混合感染则首选克拉霉素。结论泌尿生殖道感染主要以Uu为主，单独Uu和Mh感染以及二者混合感染的耐药性存在差异，治疗两种支原体单独感染分别以强力霉素和交沙霉素为首选。     

泌尿生殖道支原体检测及耐药性分析

目的：探讨性病门诊病人解脲脲原体（Uu）和人型支原体（Mh）感染及耐药情况,为临床诊断及治疗提供实验数据。方法：应用珠海黑马生物工程有限公司生产的支原体培养、鉴定计数药敏试剂盒进行试验。结果：969例标本中,支原体阳性为574例。总感染率为59.24%,其中Uu感染388例（40.04%）,Mh感染103例（10.63%）,Uu＋Mh感染83例（8.57%）。12种抗菌药物药敏结果显示,交沙霉素、强力霉素、美满霉素、四环素敏感率高。罗红霉素、阿齐霉素、环丙沙星、司巴沙星、氧氟沙星、克拉霉素、左氧沙星、红霉素敏感率低。结论：解脲脲原体Uu是非淋球菌性尿道炎感染人群的主要病原体,有多重耐药株出现,临床治疗应根据药敏结果选择敏感药物。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.