非放标AR基因多态性分析探讨子宫平滑肌瘤的克隆性

目的：根据女性体细胞中两条X染色体上雄激素受体（AR）基因的限制性片段长度多态性进行非放标克隆性检测，以探讨子宫平滑肌瘤的克隆组成。方法：自新鲜子宫平滑肌瘤组织中提取基因组DNA，经HhaⅠ消化后，PCR扩增，变性聚丙烯酰胺凝胶电泳后银染显示单股DNA片段长度，结果：在检测的54例子宫平滑肌瘤标本中，32例（59．3％）具有这种AR位点的多态性；其中3例标本X染色体失活发生了严重偏移，不适用于这种克隆性分析，对29例共68个平滑肌瘤结节的分析均显示AR位点长度多态性丢失，提示全部为单克隆细胞组成，其中8例结节个数≥3佣的多发性子宫平滑肌瘤中，6例标本的不同瘤结节均具有相同的X染色体灭活带型，另外，发现1例具有X染色体失偏移的标本其肿瘤组织中有额外条带出现，而且两个瘤结节具有相同改变，结论：此研究为国内开展非放标AR克隆性检测奠定了基础，同时还证实我们以前的研究结果，部分子宫平滑肌瘤不同瘤结节之间可能有共同的克隆起源。     

肺硬化性血管瘤整个病变的克隆性

目的:探讨肺硬化性血管瘤(PSH)整个病变的克隆性并阐明其本质.方法:利用激光显微切割技术获取22例女性肺PSH组织中多角形细胞和表面立方细胞构成的整个病变及病变周围正常肺组织,分别提取基因组DNA,通过基于女性体细胞构成组织内X染色体失活嵌合性和磷酸甘油酸激酶(PGK)基因位点的克隆性分析、观察PSH整个病变的克隆性.结果:22例肺PSH光镜下均表现为典型的组织病理学特征,即呈实性、乳头状、硬化性和出血性组织结构.病变主要由间质圆形或多角形细胞及被覆在乳头状结构表面的立方细胞所构成.克隆性检测结果表明有7例31.8%(7/22)显示PGK基因位点多态性.经HpaⅡ酶切后,所有具有多态性的标本2个条带中有1个条带的密度明显减弱或消失,而另1个条带保留,证明其均为肿瘤性增生.结论:PSH是一种真性肿瘤,构成PSH的2种主要细胞起源于同一类型细胞.     

平滑肌瘤女性患者中肿瘤坏死子-α-308启动子和p53密码子72基因的多态性

肿瘤坏死因子-α(TNF-α)是一种致炎细胞因子,在自身免疫性疾病中发挥重要的作用。p53与细胞生长调控和抑癌作用有关。本文旨在研究TN F-α-308启动子基因多态性及p53基因多态性能否作为平滑肌瘤的易感性指标。试验采用前瞻性基础研究。试验地点为医学中心的妇科和遗传学科。第1组:平滑肌瘤患者(n=159),第2组:非平滑肌瘤患者(n=131)。从外周血白细胞中获取基因组D N A。对TNF-α和p53的基因多态性行聚合酶链反应(PCR)扩增、限制性酶切和电泳检测。鉴定两种基因的多态性:①染色体6p21.3的TN F-α基因A(可酶切)/G(不可酶切)多态性。②…     

子宫富于细胞性平滑肌瘤雌、孕激素受体及Ki-67的表达及临床意义

目的 分析评价子宫富于细胞性平滑肌瘤中雌激素受体（ER）、孕激素受体（PR）、Ki-67的表达及临床意义.方法 收集2010～2014年湖北省妇幼保健院41例子宫富于细胞性平滑肌瘤、12例普通型子宫平滑肌瘤、5例恶性潜能未定子宫平滑肌瘤及10例子宫平滑肌肉瘤患者的子宫组织标本,采用免疫组化法检测各子宫组织中ER、PR以及Ki-67的表达水平.结果 ER在普通型子宫平滑肌瘤（83.3％）、富于细胞性平滑肌瘤（90.2％）以及恶性潜能未定型平滑肌瘤的阳性表达率（80.0％）逐渐下降,差异无统计学意义（P＞0.05）.ER在普通型子宫平滑肌瘤、富于细胞性平滑肌瘤以及恶性潜能未定型平滑肌瘤的阳性表达率均显著高于子宫平滑肌瘤肉瘤（10.0％）的表达,差异有高度统计学意义（P＜0.01）.PR在普通型子宫平滑肌瘤（100.0％）、富于细胞性平滑肌瘤（97.6％）以及恶性潜能未定型平滑肌瘤的阳性表达率（80.0％）逐渐下降,但差异无统计学意义（P＞0.05）.PR在普通型子宫平滑肌瘤、富于细胞性平滑肌瘤以及恶性潜能未定型平滑肌瘤的阳性表达率均显著高于子宫平滑肌瘤肉瘤（20.0％）,差异有高度统计学意义（P＜0.01）.Ki-67在普通型子宫平滑肌瘤[（0.020±0.009）个/10 HPF]、富于细胞性平滑肌瘤中[（0.039±0.011）个/10 HPF]的表达量比较,差异无统计学意义（P＞0.05）.Ki-67在普通型子宫平滑肌瘤、富于细胞性平滑肌瘤中的表达均显著低于恶性潜能未定型平滑肌瘤[（0.231±0.077）个/10 HPF]和子宫平滑肌肉瘤中[（0.320±0.103）个/10 HPF]的表达,差异有高度统计学意义（P＜0.01）.子宫富于细胞性平滑肌瘤组织中ER和PR表达没有明显的相关性（P＞0.05）,两者与Ki-67呈显著正相关（r=0.399、0.452,P＜0.05）.结论 子宫富于细胞性平滑肌瘤组织中ER和PR高表达,ER和PR的表达与Ki-67呈正相关.ER、PR和Ki-67的表     

不同位点显微切割对宫颈鳞癌及其上皮内瘤变克隆性分析的影响

目的探讨同一病例不同位点组织显微切割方法对宫颈鳞状细胞癌(squamous cell carcinoma,SCC)及其宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)克隆性检测结果的影响和意义。方法随机选取病理存档宫颈SCC 22例,CIN 48例,采用单位点或多位点显微切割方法,分别切取病变上皮组织及其相应的间质,抽提DNA,用限制性内切酶HpaⅡ酶切,聚合酶链反应(PCR)扩增人类X染色体上的雄激素受体基因第一外显子,比较酶切前后的结果,判断病变的克隆性特征。结果纯合子8例,杂合子62例,杂合率88.6%。CIN组单克隆性病变明显高于正常对照组,单位点显微切割病变的单克隆率为CINⅠ5/20(25%)、CINⅡ/Ⅲ16/22(72.7%)和宫颈SCC17/20(85%);多位点显微切割病变的单克隆率为CINⅠ3/20(15%)、CINⅡ/Ⅲ13/22(59%)和宫颈SCC12/20(60%),其检测结果差别有统计学意义(P<0.05)。多点微切割的病变中,10例呈现不同的X染色体失活类型。结论单位点显微切割的方法检测宫颈病变单克隆性比多位点显微切割更为准确,对判断CIN的肿瘤属性有意义。多位点显微切割对研究SCC发生的单中心或多中心起源有意义。本组结果提示大部分SCC起源于单个位点的单克隆改变,而部分病例为多位点的单克隆发生。     

X染色体失活和微卫星分析确定多发性子宫平滑肌瘤的独立克隆来源

目的:本研究分析了来自14例多发性子宫平滑肌瘤患者的43份样本的15个微卫星标志物的等位基因状态,并进行X染色体灭活分析,探讨子宫平滑肌瘤的肿瘤发生中的克隆性和遗传的关系。研究设计:本研究对定位于3q、7p、11及15q的15个微卫星多态性标志物进行自动性分析。通过X-连锁雄激素受体基因的甲基化状态来评估X染色体的失活。结果:15个微卫星标志物中至少有1个发生等位基因缺失,8例中有7例的杂合性缺失类型不同,在同一患者的3份样本同时监测到7p22-15存在杂合性缺失。X染色体失活分析显示12例患者中有9例患者的所有肿瘤样本中均出现了同样的…     

伴发乳头状肾细胞癌的家族性多发性皮肤和子宫平滑肌瘤

多发性皮肤和子宫平滑肌瘤是一种常染色体显性遗传综合征,可导致皮肤发生良性平滑肌瘤,在女性可发生子宫平滑肌瘤。该综合征可重叠遗传性平滑肌瘤病和肾细胞癌综合征,患者不仅发生皮肤平滑肌瘤,还可发生罕见的II型乳头状肾细胞癌。近来,上述两个综合征均发现编码延胡索酸水合酶基因杂合性突变。延胡索酸水合酶在Kreb循环中催化延胡索酸转变为苹果酸,并可能有肿瘤抑制功能。作者报道1个发生多发性平滑肌瘤、子宫平滑肌瘤和乳头状肾细胞癌的家系。先证者为女性,波兰人,77岁,30岁时发生右上臂多发性皮肤平滑肌瘤,40岁时因子宫平滑肌瘤行子宫…     

子宫平滑肌肿瘤临床病理分析

目的总结子宫平滑肌肿瘤临床病理组织学分型。方法回顾性分析300例子宫平滑肌肿瘤的临床病理资料。结果子宫肌壁间258例（86%）,浆膜下18例（6%）,黏膜下24例（8%）,平滑肌瘤276例（92%）,平滑肌肉瘤2例（0.67%）,交界性平滑肌瘤22例（7.33%）。结论良性平滑肌瘤最多见,恶性平滑肌瘤很少见,交界性平滑肌瘤将会随着诊断水平的不断提高越来越少。     

子宫富于细胞性平滑肌瘤雌激素受体孕激素受体Ki-67表达与临床病理特点相关性研究

本文利用免疫组织化学技术对32例子宫富于细胞性平滑肌瘤（cellular leiomyoma,CL）雌激素受体（ER）、孕激素受体（PR）和Ki-67进行检测,目的在于探讨ER、PR、Ki-67在不同临床病理特点子宫富于细胞性平滑肌瘤中的表达情况及在其发生发展中的意义。     

雷公藤多甙对子宫肌瘤患者子宫和肌瘤血供的影响

子宫肌瘤是女性生殖系统最常见的良性肿瘤 ,是起源于单个平滑肌细胞的单克隆性肿瘤 ,至少有 30 %的 30岁以上妇女患有肌瘤。虽然肌瘤相当常见 ,但其发病机制尚不清楚。目前认为肌瘤的发生与肌细胞遗传物质的改变有关 ,现已发现肌瘤细胞中存在多种类型的染色体异常 ,以7、12、14     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.