INFLUENCE OF ELECTROACUPUNTURE AT “NEIGUAN” ON MYOCARDIAL MORPHOLOGY IN RATS WITH CARDIAC HYPERTROPHY

Objective: To discuss the influence of electroacupuncture at "Neiguan" on myocardial morphology in rats with cardiac hypertrophy.Methods: Rats in the normal group received 3 mg/(kg·d) physiologic saline injection into the back skin, and rats in the model group received 3 mg/(kg·d) isoprenaline hydrochloride for 14 d; the electroacupuncture group received 3 mg/(kg·d) isoprenaline hydrochloride, and the acupuncture needles were penetrated 5 mm into the bilateral "Neiguan" acupoints and interconnected with a HANS electroacupuncture apparatus, in which the following stimulus parameters were selected: continuous wave at 2 Hz and 1 mA, for 20 min every day; rats in the P38 MAPK inhibitor group were injected 3 mg/(kg·d) isoprenaline hydrochloride and 0.3 mg/(kg·d) SB203580; rats in the electroacupuncture and P38 MAPK inhibitor group were injected 3 mg/(kg·d) isoprenaline hydrochloride and 0.3 mg/(kg·d) SB203580, and treated with electroacupuncture for 14 d.On the 15 th day, the body weights of the rats, as well as the weights of their left ventricle and of the whole heart were recorded; then left ventricular weight index and the heart weight index were calculated; hematoxylin-eosin staining was performed for the heart tissue.Results: Compared with the normal group, HW, LVW, HWI, and LVWI were significantly higher with statistical significance in model group(P0.01); com pared with the model group, HW, LVW, HWI, and LVWI were significantly lower with statistical significance in the electroacupuncture group, the P38 MAPK inhibitor group, the electroacupuncture and P38 MAPK inhibitor group(P0.05), but no significant difference between them(P0.05).Compared with the model group, cardiomyocyte hypertrophy was improved, myocardial fiber arrangement became neat, and edema and hyperplasia were improved significantly.Conclusion: Electroacupuncture at "Neiguan" can decrease HWI, LVWI, and the average measurement of left ventricular wall thickness, and improve myocardial morphology significantly.     

Effect of Chaiqinchengqi decoction on inositol requiring enzyme 1α in alveolar macrophages of dogs with acute necrotising pancreatitis induced by sodium taurocholate

OBJECTIVE: To investigate the effect of Chaiqinchengqi decoction(CQCQD) on inositol requiring enzyme 1α(IRE1α) in alveolar macrophages(AMs)of the dog model of acute necrotising pancreatitis(ANP) induced by sodium taurocholate.METHODS: Fifteen beagle dogs were randomised into a control group,ANP group and CQCQD group(n = 5 per group). ANP was induced by a retrograde duct injection of 50 mg/kg of 5% sodium taurocholate. The dogs in the control group received injections of the same volume of saline as the sodium taurocholate. After the models were induced,the dogs in the CQCQD group were administered 10 m L/kg CQCQD every 2 h for 6 h. Two hours after the last administration of either CQCQD or saline,they were sacrificed by anaesthesia. AMs were collected to determine the IRE1α and Interleukin-1β(IL-1β)m RNA and protein expression,and pancreatic tissues were collected for histopathology analysis.RESULTS: Compared with the ANP group,the m RNA and protein expression of IRE1α and the protein expression of IL-1β of AMs in the CQCQD group were significantly down-regulated,and the pancreatic histopathology score of the CQCQD group also was lower. There was no significant difference in the m RNA expression of IL-1β of AMs between the two groups.CONCLUSION: The CQCQD-induced down-regulation of the IL-1β protein expression may involve the down-regulation of the m RNA and protein expression of IRE1α in AMs.     

Role of Combining Spreading Moxibustion and Point Injection in Reducing ECP and LPO Levels and Improving Lung Function简

Objective： To observe the effect of combining spreading moxibustion and point injection on serum eosinophil cationic protein（ECP）, lipid peroxidase（LPO） and cellular immunity in patients with cough-variant asthma. Methods： A total of 150 cases who met the inclusion criteria were randomized into three groups, 50 in each group. Spreading moxibustion and point injection were employed in the observation group, spreading moxibustion alone in the spreading moxibustion group and point injection alone in the point injection group. Then the therapeutic efficacies were evaluated after one course of treatment. Results： The recovery rate and total effective rate were 50.0% and 98.0% respectively in the observation group, versus 18.0% and 86.0% in the spreading moxibustion group and 14.0% and 82.0% in the point injection group, showing a statistically significant difference（P 〈 0.01）. Before treatment, there were no inter-group statistically significant differences in levels of ECP, LPO, CD3＋,CD4＋, CD4＋/CD8＋ and CD8＋, forced vital capacity（FCV）, forced expiratory volume 1（FEV1）, and peak expiratory flow（PEF）（P〉0.05）. After treatment, the ECP and LPO levels in the observation group were more significantly reduced than those in the other two groups, showing statistically significant difference（P〈0.01）; the levels of CD3＋, CD4＋ and CD4＋/CD8＋ in the observation group were significantly elevated but CD8＋ was significantly reduced, showing statistically significant differences compared with those in the other two groups（P〈0.01）; and the lung function indexes（FVC, FEV1 and PEF） in the observation group were more significantly elevated than those in the other two groups（P〈0.05）. Conclusion： Combining spreading moxibustion and point injection could remarkably reduce the contents of ECP and LPO in patients with cough-variant asthma, improve cellular immunity, increase the contents of CD3＋, CD4＋ and CD4＋/CD8＋, reduce the content of CD8＋ and improve the lung function.     

Toxicity study of ethanol extract from Oroxylumindicum(L.) Vent in rats

OBJECTIVE: To evaluate the safety of Oroxylumindicum(L.) Vent extract administered for 26 weeks in Wistar rats.METHODS: Oroxylumindicum(L.) Vent extract was administrated to male and female rats by gavage once daily at doses of 54, 225, and450 mg·kg-1·d-1.The rats were sacrificed after administration for13 weeks and 26 weeks. Part of the rats in each group were allowed to recover for 4 weeks after 26-week administration. Systematic examinations including haematology, urology, blood biochemistry and histomorphology were performed at the end of13, 26 weeks of administration and 4 weeks of recovery.RESULTS: No treatment related adverse effect shappened on rats' general status, body weight, food consumption, urinary index and histomorphologyexamination. Although during the administration,in some rats of extract's groups, the value of Red blood cell count(RBC), white blood cell counts(WBC), hemoglobin(HGB), haematocrit(HCT) and K iron were decreased, and biochemistry index,such as glucose(GLU), triglyceride(TG), alanine transaminase(ALT) and blood urea nitrogen(BUN)were increased, the above parameters were within the normal ranges and all returned to baseline after the drug stopping for 4 weeks.CONCLUSION: The administration of Oroxylumindicum(L.) Vent extractat levers up to 450 mg/kg(equals to 75 times of clinical dose) is well toleratedfor both genders without significant toxicity within the administration duration in this study.     

西洋蓍草提取物对糖尿病大鼠的β细胞保护活性及降糖、降脂作用(英文)

AIM:To evaluate the therapeutic uses of Achillea millifolium in diabetic rats.METHODS:Diabetes was induced bysingle intraperitoneal injection of freshly prepared solution of alloxan monohydrate(150 mg·kg.1 body weight) in Wistar rats of150.200 g body weight.In this study,the aqueous and methanolic extract of Achillea millifolium was studied for its hypoglycemic andhypolipidemic properties.The rats were divided into several groups,serving as Normal group,Diabetic Control group,Diabetictreated with glibenclamide,and extract treated groups.The blood serum collected from the various groups of rats was analysed for itsvarious biochemical parameters like glucose,cholesterol,triglycerides,VLDL,SGOT,SGPT and ALP.On the 14th day of the experimentthe rats were scarified and pancreas was collected for histopathological studies.RESULTS:The extracts at dose levels of 250and 500 mg·kg.1 body weight showed significant(P ≤ 0.05) decrease in blood glucose level,TGL,VLDL,cholesterol,SGOT,SGPT,and ALP in diabetic rats.The extracts prevented the β-cells of pancreas from the cytotoxic effects of Alloxan monohydrate.CONCLUSION:The results indicate that the extracts as mentioned above are effective in hyperglycemia and can effectively protectagainst other metabolic aberrations caused by alloxan monohydrate.     

Jian-Gan-Xiao-Zhi decoction ameliorates high-fat high-carbohydrate diet-induced non-alcoholic fatty liver disease and insulin resistance by regulating the AMPK/JNK pathway

Background:Non-alcoholic fatty liver disease(NAFLD)can cause insulin resistance(IR)and diabetes.Our previous studies have demonstrated that Jian-Gan-Xiao-Zhi decoction(JGXZ)could be effective for the treatment of NAFLD and IR.However,the possible mechanism underlying the effects of JGXZ on NAFLD and IR remains unknown.Methods:Fifty rats received a high-fat high-carbohydrate(HFHC)diet for 12 weeks to induce NAFLD.After 4 weeks of HFHC treatment,rats were orally treated with JGXZ(8,16,and 32 g/kg weight)for 8 weeks.Ten rats in the control group received standard chow.In the positive control group,rats were orally treated with metformin(90 mg/kg weight)for 8 weeks.After JGXZ and metformin treatment,H&E staining was conducted on rat livers and serum biochemical markers,including alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglyceride(TG),and total cholesterol(TC),were measured using test kits.Moreover,a fasting blood glucose test and an oral glucose tolerance test(OGTT)were conducted.Serum levels of insulin were determined using ELISA kit,and the homeostatic model assessment of insulin resistance(HOMA-IR)was calculated.The levels of total insulin receptor substrate-1(IRS1),AMP-activated protein kinase-α(AMPKα)and c-Jun N-terminal kinase(JNK)as well as the levels of phosphorylation of IRS1(p-IRS1),phosphorylation of AMPK(p-AMPK)and phosphorylation of JNK(p-JNK)were measured using western blotting.Results:The body weights in JGXZ low-,middle-,and high-dose groups were lower than those in the model group(P<0.05,P<0.01,P<0.01,respectively).The serum levels of AST(P<0.05 in JGXZ middle-and high-dose groups),ALT(P<0.01 in JGXZ middle-dose group and P<0.05 in JGXZ high-dose group),TG(P<0.01 in JGXZ middle-and high-dose groups),and TC(P<0.01)upon JGXZ treatment were lower those than in NAFLD model rats.H&E staining showed that JGXZ treatment reduced steatosis of the hepatocytes in NAFLD model rats.JGXZ decreased the levels of fasting blood glucose(P<0.01),HOMA-IR(P<0.01),AUC(area under the curve)of the OGTT(P<0.05)and p-IRS1(P<0.01 in JGXZ middle-and high-dose groups,P<0.05 in JGXZ low-dose groups).Moreover,JGXZ regulated the hepatic AMPKα/JNK pathway in NAFLD model rats,which reflected the induction of p-AMPKαand inhibition of p-JNK.Conclusion:This study showed that JGXZ improved liver function and reduced steatosis of the hepatocytes in NAFLD model rats.Moreover,JGXZ improved IR in NAFLD model rats.The possible mechanism underlying the effects of JGXZ on NAFLD and IR involves the modulation of the AMPK/JNK pathway.     

Effect of chrysanthemum extract on myocardial fibrosis in rats with renovascular hypertension

OBJECTIVE: To investigate the inhibitory effect of chrysanthemum extract on myocardial fibrosis in rats with renovascular hypertension, and explore the possible mechanism underlying this effect.METHODS: Sixty Wistar rats were randomly divided into six groups: sham operation, model, positive control, and low-, medium-, and high-dose Huai chrysanthemum extract groups(ten rats per group). With the exception of the sham operation group, a renal hypertensive model was established in rats using the "two-kidney, one clip" method. After 6 weeks, low-, medium-, and high-dose groups were intragastrically administered chrysanthemum extract at 1, 2, or 4 g/kg, respectively, once daily for 4 weeks. The positive control group was administered Kato Pury at 50 mg/kg once daily for 4 weeks,while sham operation and model groups received an equal volume of distilled water once daily for 4 weeks. Blood pressure changes were examined before modeling, 6 weeks after modeling, and after 4 weeks of treatment administration. Ventricular remodeling indexes were measured by high frequency echocardiography after 4 weeks of treatment administration. Pathological changes were observed by hematoxylin and eosin, and Masson's trichrome staining methods. Collagen type sion were eⅠxa(Col minedⅠ) and type Ⅲ(Col Ⅲ) expres by enzyme-linked immunosorbent assays. Transforming growth factor-β1(TGF-β1), sma mad 3(Smad3),Smad7, Ras homolog gene family, member A(RhoA), and Rho-associated protein kinase 1(ROCK1) protein expression were detected by Western blot.RESULTS: Compared with the model group, chrysanthemum-administered groups and the positive control group showed significant improvement of arterial blood pressure, echocardiography indicators, and degree of myocardial fibrosis(P 0.05). In addition, these groups exhibited decreased expression of Col Ⅰ, Col Smad3, and increaseⅢ, RhoA, ROCK1, TGF-β1, and d Smad7 expression. Such improvements were most obvious in the high-dose chrysanthemum extract group(P 0.05).CONCLUSION: Chrysanthemum extract could effectively reduce myocardial fibrosis in rats with renovascular hypertension by a mechanism that potentially involves inhibition of RhoA/ROCK1 and TGF-β1/Smad signaling pathways.     

“Dose-effect-response” Relationships of Paeoniae Radix Rubra on α-Naphthylisothiocyanate-induced Acute Cholestatic Hepatitis in Rats

Objective To investigate the hepatoprotective effects of Paeoniae Radix Rubra(PRR)at different doses against α-naphthylisothiocyanate(α-NIT)-induced acute cholestatic hepatitis in rats.Methods Rats were ig admini-strated with vehicle or PRR[(1,9,18,36,54,72,and 81 g/(kg·d)]3 d before and 2 d afterα-NIT(60 mg/kg)ig administration.The general status of rats,histopathology of liver,serum alanine aminotransaminase,aspartate aminotransaminase,total bilirubin,direct bilirubin,and alkaline phosphatase levels,were observed at respective time points(24 and 48 h)afterα-NIT administration.Using cluster analysis and correspondence analysis,the "dose-effect-response"relationships of PRR were evaluated.Results The results showed that compared with model group,the serum biochemistry index significantly decreased with the increasing of PRR dosage(P<0.01), and the change and necrosis of hepatic cellula,and inflammatory cell infiltration were gradually alleviated. However,the improvement was not obviously found in the low-dose group[1 g/(kg·d)].The cluster analysis and correspondence analysis results showed that different doses of PRR could significantly ameliorateα-NIT-induced acute cholestatic hepatitis of rats in a dose-dependent manner.Conclusion The experiments show that administration doses of PRR in clinical use should be added properly in order to gain the expectant therapeutic effect,especially in the treatment of heavy acute cholestasis hepatitis.     

Effect of Liuweidihuang pill and Jinkuishenqi pill on inhibition of spontaneous breast carcinoma growth in mice

OBJECTIVE: To investigate the preventing and treating action of Liuweidihuang pill(LP) and Jinkuishenqi pill(JP) on spontaneous breast carcinoma in mice.METHODS: A model of spontaneous breast carcinoma was derived from 11.5-month-old female Kunming breeding mice following the delivery of several litters. The mice were randomly divided into five groups: model control group(C),Liuweidihuang pill high-dose group(LH; 4.6 g·kg1·d1),Liuweidihuang pill low-dose group(LL;2.3 g·kg1·d﹣1),Jinkuishenqi pill high-dose group(JH; 4.6 g·kg﹣1·d1) and Jinkuishenqi pill low-dose group(JL;2.3 g·kg﹣1·d﹣1). Cancer tissue volume was measured by water immersion. Histopathology was analyzed by hematoxylin and eosin staining. Vascular endothelial growth factor(VEGF),extracellular signal-regulated kinase(ERK) and cyclin D1 protein expression in cancer tissue was assayed by western blotting.RESULTS: Compared with the control group,cancer tissue volume and weight were lower in the LP and JP groups,and survival time was longer. The expression of VEGF,ERK and Cyclin D1 were inhibited in the LP and JP groups(P 0.05),and cell differentiation was increased. Tumor weights and volumes and VEGF,ERK and Cyclin D1 expression in LL or LH were significantly lower than in JL and JH(P 0.01).CONCLUSION: Both LP and JP could restrain cancer growth and promote cancer cell differentiation;moreover,LP was more effective than JP The likely mechanism of action was via inhibition of VEGF,ERK and cyclin D1.     

金顶侧耳提取物拮抗环磷酰胺致小鼠肝损伤作用的研究

目的探讨金顶侧耳提取物拮抗环磷酰胺致小鼠肝损伤的作用。方法小鼠以环磷酰胺(85 mg/kg×2)腹腔注射制备肝损伤模型,金顶侧耳提取醇沉上清部分按1.6,0.8,0.4 g/(kg.d)分为3个剂量组,沉淀部分按0.8,0.4,0.2 g/(kg.d)分为3个剂量组,腹腔注射给药(正常对照组注射生理盐水)13 d后测定各组小鼠血清中丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)的活性和丙二醛(MDA)的含量及肝组织MDA含量。结果与正常对照组比较,模型组小鼠ALT、AST和MDA含量明显提高(P均<0.05);与模型组比较,金顶侧耳提取物沉淀部分0.8 g/(kg.d)组和0.4 g/(kg.d)组小鼠ALT和AST的活性明显降低(P<0.05或0.01);上清部分0.4 g/(kg.d)组小鼠血清AST的活性明显降低(P<0.05);金顶侧耳提取物沉淀部分0.4 g/(kg.d)组和上清部分0.4 g/(kg.d)组小鼠血清MDA含量明显降低(P均<0.05)。结论金顶侧耳提取物具有一定的拮抗环磷酰胺致小鼠肝损伤的作用,其中沉淀部分的生物活性较强。     

Protection of Water Extract from Paeoniae Radix Rubra against Myocardial Ischemia in Mice Induced by Isoproterenol

Objective To study the protective effect of the water extract from Paeoniae Radix Rubra(WEPRR)against myocardial ischemia in mice induced by isoproterenol(ISO).Methods The mice were randomly divided into six groups:positive control,normal control,model,low-,mid-,and high-dose[0.3,0.6,and 1.2 g/(kg·d)]WEPRR groups.The mice in WEPRR groups were ig administered with WEPRR,the mice in the positive control group were ig administered with 0.98%Di’ao Xinxue Kang[(0.195 g/(kg·d)],and the mice in normal and model groups were ig administered with the same volume of physiological saline once daily for consecutive 11 d.On the day 7 from the beginning of the ig administration,the mice in the model and WEPRR groups were ip perfused with 0.02 g/(kg·d)ISO.After 15 min of the last medication,the mice were anesthetized with isoflurane gas,the blood was collected through venous sinus of eye orbit,then the mice were killed.The heart tissues were rapidly removed from the mice,washed in physiological saline,soaked in filter paper,and stored in 80℃until use.The activities of lactate dehydrogenase(LDH),creatine kinase-MB(CK-MB),creatine kinase(CK)in serum,superoxide dismutase(SOD),and the contents of malondialdehyde(MDA)in the heart of mice were determined,respectively.Results Compared with the model group,the activities of LDH,CK-MB,and CK in serum,and the SOD of mice in the positive control and WEPRR groups were increased and the content of MDA in heart was decreased.Conclusion The WEPRR has the cardioprotective activities on ISO-induced myocardial ischemia.     

三妙丸抗下肢丹毒复发的实验研究

目的 :研究三妙丸对小鼠免疫系统及炎症反应的影响。方法 :用碳粒廓清实验研究药物对小鼠网状内皮系统的影响 ;观察药物对 2 ,4二硝基氯苯 (DNCB)诱导的迟发性超敏反应 (DTH)及小鼠血清溶血素含量的影响 ;用二甲苯耳廓肿胀法和棉球肉芽肿法观察药物对炎症反应的影响。结果 :三妙丸及苍术醇提胶囊能增加小鼠网状内皮系统的吞噬功能 ,增强小鼠迟发性超敏反应 ,提高血清溶血素含量及免疫器官重量 ;能对抗二甲苯所致耳廓肿胀、抑制肉芽的增生。结论 :三妙丸有抗炎及免疫调节作用 ,可能是其抗下肢丹毒复发的药理作用基础。     

息肿胶囊对心力衰竭大鼠RAS的影响

目的观察息肿胶囊对心力衰竭大鼠心功能的影响。方法80只大鼠随机分为正常对照组10只,余70只随机分为模型组、双氢克尿噻组10 mg/(kg.d)组、息肿胶囊3.75 g/(kg.d)组、息肿胶囊7.5 g/(kg.d)组、息肿胶囊15 g/(kg.d)组。正常对照组和模型组给予生理盐水10 mL/k g,其余各组腹腔注射盐酸阿霉素15 mg/kg,每周一、四和周日各给药1次,2周内注射完毕。各组在造模的同时给予治疗药物,每天给药1次,连续用药14 d。给药结束后次日,用放射免疫法测定血浆中AngⅠ、AngⅡ和ALD的含量。结果模型组血浆中AngⅠ、AngⅡ和ALD浓度明显升高,与对照组比较有极显著性差异(P均<0.01)。双氢克尿噻组与息肿胶囊组均能降低由阿霉素所致的血浆中AngⅠ、AngⅡ和ALD的浓度,与模型组比较有显著性差异(P<0.05),特别是息肿胶囊高剂量组血浆AngⅠ、AngⅡ含量与模型组比较有极显著性差异(P均<0.01)。结论息肿胶囊能降低由阿霉素所致心力衰竭大鼠血浆中AngI、AngII和ALD的浓度,为其抗充血性心力衰竭的机制之一。     

Screening of Phytoestrogenic Effective Extracts and Dose of Cistanche deserticola

Objective To screen the phytoestrogenic effective extracts and dose of Cistanche deserticola including estrogenic and anti-estrogenic activities.Methods The effect of phytoestrogen was determined through uterus growth test in low and high estrogen female model mice.Then MTT assay of the estrogen-dependent breast cancer cells MCF-7 was conducted with the medicated serum of mice.Results After ig administration with 95%ethanol extract of C.deserticola[EECD,30 g/(kg·d)],the uterus coefficient of low estrogen model mice increased.The medicated serum of 30 g/(kg·d)EECD significantly promoted the proliferation of MCF-7 cells.40 g/(kg·d)EECD+diethylstilbestrol significantly inhibited the growth of the uterus in high estrogen model mice and the proliferation of MCF-7 cells as well.Conclusion With the dose of 30 g/(kg·d),EECD could exert quasi estrogen effect,and with the dose of 40g/(kg·d),EECD could exert the estrogen antagonistic action.The method established is accurate and reliable,which could be used for the follow-up studies on the phytoestrogen material basis of C.deserticola.     

大鼠静脉注射红花提取物毒性观察

目的观察长期给予红花提取物的毒理作用。方法分别给4组大鼠静脉注射红花提取物(400 mg/(kg.d)2、00 mg/(kg.d)、80 mg/(kg.d))及生理盐水,连续6周,对其体质量、摄食量、饮水量、血液学指标、血液生化指标、主要脏器组织学等进行检测。结果400 mg/(kg.d)出现中毒反应,主要表现为对胆碱能系统有兴奋作用,并对锥体外系有一定影响。80mg/(kg.d)为无毒剂量,200 mg/(kg.d)为安全剂量。结论长期给予红花提取物的毒理作用呈剂量相关特性。     

大剂量岷当归对高同型半胱氨酸血症致兔动脉粥样硬化的影响

目的:探讨大剂量岷当归对高同型半胱氨酸血症(HHcy)致兔动脉粥样硬化(AS)的影响。方法:将日本大耳白兔48只随机分为正常组、模型组、叶酸组及岷当归低、中、高剂量组,每组8只。采用L-蛋氨酸皮下注射法建立HHcy家兔模型。造模8周后,岷当归低、中、高剂量组分别给予1.4、2.8、5.6 g/(kg·d)的岷当归水煎液灌胃治疗,叶酸组给予叶酸1.5 mg/(kg·d)、维生素B620 mg/(kg·d)、维生素B120.25 mg/(kg·d)灌胃治疗。12周后检测各组血清同型半胱氨酸(Hcy)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)水平,同时观察兔腹主动脉血管超声与病理变化。结果:模型组兔血清Hcy水平高于正常组,差异有统计学意义(P0.05),HHcy模型形成;岷当归低、中、高剂量组及叶酸组血清Hcy水平与模型组比较下降,差异有统计学意义(P0.05);岷当归低、中、高剂量组血清TC、TG、LDL-C水平低于模型组,差异有统计学意义(P0.05);腹主动脉超声与组织学检查发现,模型组有明显的AS征象,各治疗组病变程度较模型组减轻。结论:大剂量岷当归通过降低血清中Hcy水平,调节脂肪代谢紊乱,减轻HHcy对血管内皮的损伤,对HHcy所致的AS病变起到治疗作用。     

梅山降糖神茶对四氧嘧啶糖尿病大鼠的降糖作用

 目的：研究梅山降糖神茶对四或嘴吮糖尿病大鼠的降糖作用。方法：给四氧咭咤糖尿病大鼠连续4周ig梅山降糖神茶3 g/(kg·d),6 g/(kg·d)后，浏定其空腹血糖、糖耐贡试验W及切片观察眺岛形态。结果：给药2周后，空腹血粉显著降低;4周后糖耐量能力明显增强;病理切片结果提示：胰岛形态、结构明显修复。结论：梅山降糖神茶对四乳啥吮糖尿病大鼠有标本兼治之功效。     

黄芪生脉饮对在体猪急性心肌缺血的保护效应及其可能机制

目的　观察黄芪生脉饮对在体小型猪急性心肌缺血的保护效应并探讨其可能机制。方法　 2 4只小型猪随机分 4组 ,即空白对照组 (生理盐水 ,0 .7g/ (kg·d) )、黄芪生脉饮小剂量组 (0 .7g/ (kg·d) )、黄芪生脉饮大剂量组 (2 .1g/ (kg·d) )、生脉饮组 (0 .7g/ (kg·d) )。连续灌服 7d并记录整体猪出现的反应 ,观察其心脏血流动力学 (BP ,LVSP ,LVEDP)变化及梗死心肌占心室质量的百分比。结果　黄芪生脉饮组小型猪均未出现异常反应 ,冠脉结扎后黄芪生脉饮组与生脉饮组BP ,LVSP的降低和LVEDP的升高程度均明显优于空白对照组。空白对照组梗死区占心室总质量 (6 .2 3± 0 .39) % ,黄芪生脉饮大剂量组、生脉饮组分别为 (5 .5 5± 0 .4 7) %和(5 .6 7± 0 .35 ) % ,与空白对照组相比有显著性差异 (P均 <0 .0 5 )。结论　黄芪生脉饮能显著缩小在体猪急性心肌缺血模型的心肌梗死范围 ,改善心脏血流动力学紊乱 ,为黄芪生脉饮应用于急性心肌缺血的治疗提供一定的理论依据     

补肾益智方对老年性痴呆模型动物空间探索学习记忆功能的改善作用

目的观察补肾益智方对老年性痴呆(AD)模型大鼠空间探索学习记忆功能的改善作用.方法用15月龄老年Wistar大鼠80只,以D-半乳糖ip4周加上鹅膏蕈酸(ibotenic acid,IBO)脑内Meynert核注射制造AD模型,随机分成AD模型组,双益平治疗对照组,补肾益智方高、低剂量(12,6 g/kg@d)治疗组.另加上正常老年组和正常青年组总共设立6个组别.动物经治疗处理4周后,应用Morris水迷宫检测大鼠学习记忆的改善情况.结果空间探索试验发现,模型组及双益平组在空间探索试验中的指标与正常青年组和正常老年组有显著差异性(P＜0.01或P＜0.05);补肾益智方高、低剂量组也与模型组和双益平组有显著性差异(P＜0.01或P＜0.05).与模型组比较,正常青年组、正常老年组和补肾益智方高剂量组第7天撤离平台后大鼠在原平台象限内游泳的时间占整个游泳时间的百分比明显要高(P＜0.05),而补肾益智方低剂量和双益平组虽然要高些,但差异无显著性(P＞0.05).结论补肾益智方能明显改善AD大鼠空间探索学习记忆功能.