赤雹根总皂苷对AA大鼠血清TNF-α与IL-10含量的影响

目的:通过观察赤雹根总皂苷(TSTDR)对佐剂性关节炎大鼠血清炎性细胞因子TNF-α与IL-10含量的影响,探讨TSTDR治疗类风湿性关节炎的作用机制。方法:60只大鼠随机分组:正常对照组,模型组,TSTDR低、中、高剂量组和雷公藤多苷组,每组10只大鼠。建立佐剂性关节炎大鼠模型后,TSTDR低、中、高剂量组大鼠分别灌胃给予不同剂量的TSTDR(40mg/kg/d、80mg/kg/d和160mg/kg/d),连续21d;雷公藤多苷组大鼠灌胃给予雷公藤多苷(12mg/kg/d),时间同TSTDR组大鼠。分别计算各组大鼠的关节炎指数,ELISA法检测各组大鼠血清TNF-α和IL-10的含量。结果:TSTDR各剂量组大鼠的关节炎指数、血清TNF-α含量显著低于模型组(P<0.01,P<0.05),TSTDR各剂量组大鼠血清IL-10含量显著高于模型组(P<0.01)。结论:TSTDR对大鼠佐剂性关节炎具有治疗作用,机制可能与降低促炎性因子TNF-α水平、提高炎性抑制因子IL-10水平有关。     

昆明山海棠对胶原性关节炎大鼠免疫功能的干预作用及其机制

目的: 观察昆明山海棠(THH)对胶原性关节炎(CIA)大鼠免疫功能的干预作用,探讨其可能的作用机制。 方法: SD大鼠50只,随机分为正常组(n=10)和CIA模型制备组(n=40),用鸡Ⅱ型胶原(CⅡ)诱导大鼠制备CIA模型。再将造模成功的36只大鼠随机分为模型组、地塞米松组及200和400 mg·kg^-1THH干预组。在实验过程中观察各组大鼠足爪肿胀度和关节炎指数(AI)等变化。实验第35天,采用ELISA法检测血清中抗CⅡ抗体水平;MTT法检测脾T细胞和B细胞增殖活性;ELSIA法检测脾组织白细胞介素12(IL-12)、IL-23和肿瘤坏死因子α(TNF-α)水平;取各组大鼠足爪组织行HE染色,观察组织病理学变化。 结果: 与正常组比较,模型组大鼠AI明显上升(P<0.01),血清抗CⅡ抗体水平、脾T细胞和B细胞增殖活性、脾组织中IL-12、IL-23和TNF-α水平明显升高(P<0.01);大鼠足爪皮下组织细胞排列紊乱且有大量炎性细胞浸润及血管增生。与模型组比较,400 mg·kg^-1THH干预组大鼠一般状况得到明显改善,足爪肿胀度和AI明显下降(P<0.01),脾T细胞和B细胞增殖活性明显下降(P<0.01),血清中抗CⅡ抗体水平明显降低(P<0.01),脾组织中IL-23、TNF-α和IL-12水平均明显降低(P<0.01),大鼠足爪组织病理学改变明显减轻。200 mg·kg^-1THH组大鼠上述指标无明显变化(P>0.05)。 结论: 高剂量(400 mg·kg^-1)THH对CIA大鼠关节炎有明显抑制作用,其机制可能是THH能明显抑制T和B淋巴细胞的增殖反应,并抑制脾组织中IL-23、TNF-α和IL-12和血清中抗CⅡ抗体水平,进而明显改善大鼠足爪组织病理学变化。     

金荞麦药酒对佐剂性关节炎大鼠氧自由基和免疫功能的影响

目的 研究金荞麦药酒对佐剂性关节炎模型大鼠的治疗效果,探讨其作用机制.方法 将大鼠随机分为正常组、模型组、阳性对照组(每天灌胃雷公藤多苷片混悬液0.03 g/kg),低、中、高剂量金荞麦组(每天灌胃金荞麦药酒0.54、1.08、2.16 g/kg).建立佐剂性关节炎模型12 d后给药21 d,观察大鼠左后足跖肿胀体积,检测血清肿瘤坏死因子-α(TNF-α)、超氧化物歧化酶(SOD)、丙二醛(MDA)和足跖软组织TNF-α、前列腺素E2(PGE2)、一氧化氮(NO)含量;流式细胞术分析脾脏淋巴细胞凋亡率,免疫组化法检测膝关节滑膜巨噬细胞密度.结果 与正常组比较,模型组足跖肿胀体积、血清TNF-α、MDA和足跖软组织TNF-α、PGE2、NO水平、膝关节滑膜巨噬细胞密度明显升高(P<0.01),血清SOD、脾脏淋巴细胞凋亡率明显降低(P<0.01).与模型组比较,金荞麦可明显降低足跖肿胀体积、TNF-α、PGE2、NO水平和膝关节滑膜巨噬细胞密度,升高脾脏淋巴细胞凋亡率(P<0.05或P<0.01),但SOD、MDA无明显变化(P>0.05).结论 金荞麦药酒治疗类风湿关节炎的机制可能与调节TNF-α、PGE2、NO水平、膝关节滑膜巨噬细胞密度、脾脏淋巴细胞凋亡率有关.     

蒙药复方森登-4抗佐剂型关节炎作用的研究

目的 研究蒙药复方森登-4全方有效部位对佐剂型关节炎(AA)模型治疗效果,为其进一步开发利用奠定基础.方法 SD大鼠随机分成蒙药复方森登-4有效部位组、雷公藤组、塞来昔布组、模型组、正常组.除正常组以外,所有组采用完全弗氏佐剂导致关节炎模型,二次免疫以后给药;用自制足爪容积测量器测定每只大鼠的双足体积,计算大鼠足爪的肿胀度;用ELISA试剂盒检测血清中肿瘤坏死因子-α(TNF-α)、前列腺素-2(PGE2)及白细胞介素-6(IL-6)炎性因子的水平.结果 给药20 d后,蒙药复方森登-4有效部位组、塞来昔布组大鼠足肿胀度与模型组比较有非常显著的差异(P＜0.01),而雷公藤组大鼠的足肿胀度与模型组比较有差异(P＜0.05);这3组治疗组与正常大鼠的足肿胀度没有显著性差异.在检测TNF-α、PGE2、IL-6的水平时,雷公藤组、塞来昔布组与模型组相比有差异(P＜0.05),而蒙药复方森登-4有效部位组与模型组比较差异显著(P＜0.01).结论 蒙药复方森登-4对类风湿性关节炎不同的炎症因子有很强的抑制作用,而类风湿性关节炎的炎症因子降低与类风湿性关节炎的有效治疗正相关,所以蒙药复方森登-4可以有效治疗类风湿性关节炎.     

新风胶囊对佐剂关节炎大鼠外周血血小板活化因子及白细胞介素6和17表达的影响

目的：观察佐剂关节炎大鼠外周血血小板活化因子（platelet-activating factor,PAF）、白细胞介素6（interleukin-6,IL-6）、白细胞介素17（interleukin-17,IL-17）水平的变化及新风胶囊对其的影响。方法：将40只雄性Sprague-Dawley大鼠随机分为正常对照组、模型对照组以及甲氨喋呤（methotrexate,MTX）、雷公藤多苷片（Tripterygium Wilfordii polycoride Tablet,TPT）和新风胶囊治疗组,每组8只。除正常对照组外,大鼠右后足跖皮内注射弗氏完全佐剂0.1mL诱发大鼠关节炎。模型成功后,各组采用相应药物治疗30d。采用酶联免疫吸附法检测每组大鼠外周血PAF、IL-6和IL-17的含量,并观察各组大鼠体质量的变化,计算足跖肿胀度和关节炎指数。结果：与正常对照组相比,模型对照组大鼠外周血PAF、IL-6、IL-17含量,足跖肿胀度,关节炎指数显著升高（P〈0.01）。与模型组相比,各治疗组大鼠PAF、IL-6、IL-17含量,足跖肿胀度,关节炎指数显著降低（P〈0.01）。与MTX组、TPT组相比,新风胶囊组大鼠体质量显著升高,外周血PAF含量显著降低（P〈0.05）。佐剂关节炎大鼠外周血PAF含量与外周血IL-6、IL-17含量,足跖肿胀度,关节炎指数呈正相关（P〈0.01）。结论：佐剂关节炎大鼠外周血PAF含量升高,并与IL-6、IL-17水平,足跖肿胀度,关节炎指数具有相关性。新风胶囊通过下调PAF的水平,抑制血小板活化引起的炎症反应,降低IL-6、IL-17,从而降低佐剂关节炎大鼠的足跖肿胀度、关节炎指数。     

姜黄素衍生物FM0807对佐剂性类风湿性关节炎模型大鼠疗效观察

目的观察姜黄素衍生物FM0807对佐剂性类风湿性关节炎模型大鼠的疗效。方法取雄性SD大鼠,用弗氏完全佐剂诱导制作类风湿性关节炎大鼠模型,随机分为正常对照组、模型组对照组、雷公藤组、姜黄素衍生物FM0807低、中、高剂量组,观察关节炎症指标及血清中TNF-α、IL-1、IL-6的水平。结果姜黄素衍生物FM0807各组能降低血清中TNF-α、IL-1、IL-6的水平,中、高剂量组能有效降低关节炎症指标,呈剂量依赖关系。结论姜黄素衍生物FM0807对佐剂性类风湿性关节炎大鼠有较好疗效。     

蛇床子素对佐剂性大鼠关节炎模型治疗作用研究

目的:探讨蛇床子素对佐剂性关节炎大鼠的治疗作用,并对其作用机理进行初步研究。方法:采用弗氏完全佐剂诱导大鼠关节炎模型,测量大鼠左后足体积,检测血清TNF-α和IL-10水平,观察大鼠关节滑膜病理改变,对外周血进行T淋巴亚群分析。结果:与模型组比较,蛇床子素高剂量组大鼠左后足体积显著降低(P0.05);蛇床子素治疗组CD4+/CD8+升高,继发侧关节滑膜表面光滑,血清中炎症相关因子TNF-α和IL-10水平降低(P0.05,P0.01)。结论:蛇床子素具有改善佐剂性关节炎的作用,这可能与其具有抗炎作用相关,为研究与开发治疗类风湿性关节炎的药物提供一定的参考。     

鼠麴草对大鼠佐剂性关节炎的免疫调节作用

目的研究鼠麴草对大鼠佐剂性关节炎(AA)的抗炎消肿作用、相关炎症因子以及大鼠免疫功能的影响。方法采用注射弗氏完全佐剂(FCA)进行AA大鼠造模,造模成功的大鼠随机分为正常组、模型组和鼠麹草低、中、高剂量组、雷公藤多苷组。考察AA大鼠关节足胀度及胸腺、脾脏指数,采用酶联免疫吸附法(ELISA)测定大鼠血清中的前列腺素E_2(PGE_2)、肿瘤坏死因子(TNF-α)及白细胞介素1β(IL-1β)的水平流式细胞术(FCM)检测大鼠脾脏T淋巴细胞亚群的比例。结果与模型组比较,鼠麴草低、中、高剂量组均可以显著降低大鼠踝关节肿胀率(P0.05,P0.01),大鼠血清中PGE_2、IL-1β、TNF-α的含量明显降低(P0.01),脾脏系数、胸腺系数明显降低,差异有统计学意义(P0.05,P0.01),可显著抑制AA大鼠脾脏CD4~+,CD8~+淋巴细胞的增殖(P0.05 P0.01)升高CD4~+/CD8~+细胞比例。结论鼠麴草能抑制AA模型大鼠的足肿胀度,降低血清中炎症细胞因子水平,并改善异常的免疫功能,其机制可能与其调节CD4~+/CD8~+的平衡有关。     

督脉加药发泡疗法治疗大鼠佐剂性关节炎的研究

目的观察督脉加药发泡疗法对大鼠佐剂性关节炎(AA)的疗效,并初步探讨其作用机理。方法采用大鼠足跖皮内注射福氏完全佐剂建立AA模型,采用雷公藤膏、斑蝥膏、斑蝥雷公藤膏督脉穴位贴敷(发泡)疗法,观察足跖肿胀度、足爪组织病理学变化、脾脏指数、血清和足跖炎症组织中的PGE2含量。结果斑蝥膏组、斑蝥雷公藤膏组可有效抑制AA大鼠足跖肿胀度和足爪组织病理学变化,尤其以斑蝥雷公藤膏组为更显著。发泡疗法可提高大鼠脾脏指数值以及血清中的PGE2含量,而足跖炎症组织中PGE2含量各组间无明显差异。结论督脉发泡疗法对AA具有良好的抗炎消肿作用,在发泡剂中添加雷公藤后疗效更佳,其机理可能与免疫调节有关,与血清和足跖炎症组织中的PGE2含量变化无关。     

赤雹根总皂苷对佐剂性关节炎大鼠血清OPG/RANKL的影响

目的:观察赤雹根总皂苷(TSTDR)对佐剂性关节炎模型大鼠血清骨保护素(OPG)/核因子-κB受体活化因子配体(RANKL)的影响。方法:SD大鼠随机分为正常对照组、模型组、TSTDR(160、80、40mg/kg/d)组和雷公藤多苷(12mg/kg/d)组,每组6只大鼠。模型组、TSTDR组和雷公藤多苷组大鼠建立佐剂性关节炎大鼠模型后,各用药组大鼠灌胃给予相应药物21d。采用ELISA法检测大鼠血清OPG和RANKL水平,并计算二者的比值。结果:TSTDR各剂量组大鼠血清OPG/RANKL比值显著高于模型组(P<0.01)。结论:TSTDR可能通过影响OPG/RANKL系统抑制骨破坏,从而有助于控制类风湿性关节炎的发生发展。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.     

Possible Spread of adenovirus type 3 from poultry to humans ：indirect evidence from an outbreak in China

Objective:To explore the epidemiology and etiology for an outbreak of acute respiratory tract infection that occurred in one county of Jiangsu Province, China 2004. Methods: Only cases meeting the case definition were included in the study. We reviewed the medical records of the cases who were admitted to the local hospitals, interviewed cases by a standard questionnaire, and then described the epidemiotogic features and analyzed risk factors by means of a case-control study. We collected pharyngeal swab specimens and sent them to different laboratories for isolation and culture. The laboratory used different detection methods such as DIP, PCR, electron microscope examination and microneutralization assay, to identify and then type the positive specimens. Results:A total of 871 cases were reported during the period from April 18 to July 4,2004. The distribution of onset times presented two peaks, one in late May and another in middle June. The epidemic occurred mainly in the elementary and junior high schools in ten townships of one county, and the mean age of the cases was 12 years (range 7 months to 18 years). The course of the disease was acute, and was characterized by fever accompanied with sore throat and tonsillitis. The WBC count of cases was normal or elevated. The mean duration of illness was 5 days (range 2 to 12 days). No fatalities from illness were reported. A case-control study indicated that the possible risk factors were close contact with a case and/or poultry before onset and sharing of towels among members of the family. The typical CPE was observed through inoculating pharyngeal swab specimens into the HEP-2 cell cultures in different laboratories. An infection of adenovirus type 3 was verified by detecting positive specimens in different methods. Conclusion:This investigation demonstrated that the acute respiratory infection in cases was caused by adenovirus type 3. Cases occurred in over 70 schools in ten townships in 2004, and the route of transmission was possibly close contact with cases or droplet transmission.