草酸铈与姜辣素止呕作用研究

目的利用新型水貂呕吐模型研究草酸铈与姜辣素对顺铂所致水貂呕吐模型的治疗作用。方法雄性水貂24只随机分成4组:模型组、昂丹司琼组、草酸铈与姜辣素低剂量组,草酸铈与姜辣素高剂量组,各组分别给予生理盐水、昂丹司琼、草酸铈与姜辣素组合物低剂量与高剂量预处理,30分钟后给予腹腔注射顺铂,观察顺铂注射后6小时内水貂呕吐潜伏期和干呕、呕吐次数。结果组合物低剂量组和高剂量组水貂的呕吐潜伏期均显著长于模型组(P值均<0.01),干呕和呕吐次数均显著小于模型组(P<0.01)。结论草酸铈与姜辣素组合物对顺铂所致的水貂呕吐具有明显的止吐作用。     

生姜陈皮汤对顺铂所致水貂呕吐模型的治疗作用

目的 研究生姜陈皮汤对顺铂所致水貂呕吐模型的治疗作用.方法 雄性水貂随机分为5组:正常对照组、模型对照组、昂丹司琼组、生姜陈皮汤低剂量组和高剂量组,各组分别给予生理盐水、生理盐水、昂丹司琼、生姜陈皮汤低剂量和高剂量处理两天,第2天处理后30 min除正常对照组外各组水貂给予腹腔注射顺铂,观察注射顺铂后6 h内水貂呕吐潜伏期,干呕及呕吐次数.结果 与模型组比较,生姜陈皮汤高、低剂量组和昂丹司琼组均能显著延长呕吐潜伏期(P<0.05,P<0.01),并明显减少干呕及呕吐次数(P<0.01).结论 生姜陈皮汤对顺铂所致的水貂呕吐具有治疗作用.     

白豆蔻汤和丁香汤对顺铂所致水貂呕吐模型的治疗作用

目的 利用新型水貂呕吐模型研究白豆蔻汤和丁香汤对顺铂所致水貂呕吐模型的治疗作用.方法 雄性水貂30只随机分成5组:正常对照组、模型组、昂丹司琼组、白豆蔻组和丁香组,各组分别给予生理盐水、生理盐水、昂丹司琼、白豆蔻汤和丁香汤处理2天,第2天给予腹腔注射顺铂,观察顺铂注射后6小时内水貂呕吐潜伏期和干呕、呕吐次数.结果 白豆...     

几种致吐药物比较优选顺铂剂量造家鸽化疗呕吐模型

目的:优选顺铂造家鸽化疗呕吐模型的最佳剂量,确保造模后家鸽能正常存活,为化疗呕吐实验研究提供一种稳定可靠的造模方法。方法:仿文献采用家鸽腹腔注射顺铂的方法,与传统的致吐剂硫酸铜和另一种化疗常用药环磷酰胺比较,优选顺铂剂量进行重复试验,连续观察30天。结果:一次性给予4.5mg·kg-1是顺铂致家鸽化疗呕吐模型的最佳剂量。结论:优选的顺铂剂量4.5mg·kg-1造家鸽化疗呕吐模型能够确保化疗后适宜呕吐,也能使化疗后家鸽正常存活,符合临床上化疗引起呕吐的致病特点。优选剂量致家鸽化疗呕吐模型重复性好、稳定、可靠,这对于化疗呕吐的实验研究有意义。     

小半夏加茯苓颗粒抗顺铂所致家鸽呕吐的药效学观察

目的:比较小半夏加茯苓颗粒不同剂量抗化疗呕吐家鸽的药效以及与原方汤剂的药效差异。方法:用顺铂造家鸽急性化疗呕吐模型和延迟性化疗呕吐模型;随机分为空白组Ⅰ、模型组Ⅱ、盐酸昂丹司琼阳性对照组Ⅲ、小半夏加茯苓汤剂组Ⅳ、小半夏加茯苓颗粒抗延迟性化疗呕吐模型组Ⅷ以及小半夏加茯苓颗粒小剂量Ⅴ、中剂量Ⅵ、大剂量Ⅶ组;以呕吐潜伏期、呕吐次数和呕吐频率为指标观察各组的呕吐情况。结果:与Ⅱ组比较,Ⅲ组、Ⅳ组和Ⅴ组发生呕吐次数最少(P<0.05),Ⅷ组和Ⅵ组较多(P<0.05),Ⅶ组发生呕吐的次数最多(P>0.05)。结论:小半夏加茯苓颗粒和小半夏加茯苓汤小剂量均可显著对抗顺铂所致家鸽急性呕吐,二者药效无显著性差异,其小剂量对延迟性呕吐也有对抗作用。     

咪达唑仑对顺铂诱发猫呕吐的预防作用

目的 探讨咪达唑仑预防顺铂诱发猫恶心、呕吐的效果.方法 健康杂种家猫18只,随机分为3组：单纯模型组（顺铂组,C组）、格拉斯琼组（G组）和咪达唑仑组（M组）,每组6只,分别静注生理盐水3ml、格拉斯琼0.15mg/kg和咪达唑仑0.50mg/kg,20min后顺铂3mg/kg腹腔注射.观察动物镇静程度和10h内恶心、呕吐情况.结果 顺铂能诱发猫产生严重的恶心、呕吐反应;G组和M组猫干呕潜伏期延长,恶心、呕吐次数减少（P＜0.01）.结论 猫静脉注射咪达唑仑0.5 mg/kg能预防顺铂诱发的恶心、呕吐.     

乌灵菌粉抗大鼠戊四唑诱导癫痫的作用

目的:研究乌灵菌粉对大鼠戊四唑的癫痫点燃过程和点燃后学习能力的影响及其可能的机制.方法:将SD大鼠随机分为4组:戊四唑点燃模型组(模型对照组),低剂量乌灵茵粉组(0.3 g.kg-1),高剂量乌灵菌粉组(0.6 g.kg-1)和空白对照组.各组大鼠隔日腹腔注射亚惊厥剂量戊四唑(35 mg·kg-1),空白对照组注射生理盐水,共12次.乌灵菌粉于每次戊四唑注射前30 min灌胃.采用放射状八臂迷宫(四臂放食物)测试大鼠学习能力.化学荧光法测定大鼠脑内组胺含量.结果:(1)与模型对照组相比,低剂量乌灵菌粉组第7次注射戊四唑后发作级别显著降低,第6次注射戊四唑后肌阵挛潜伏期明显延长,第7次注射戊四唑后癫痫泛化潜伏期明显延长(P＜0.05);高剂量乌灵菌粉组第6、7和8次注射戊四唑后癫痫发作级别明显降低,第8和9次注射戊四唑后肌阵挛潜伏期显著延长,第6次注射戊四唑后癫痫泛化潜伏期明显延长(P＜0.05);(2)与空白对照组相比,模型对照组在放射状八臂迷宫训练3天后工作记忆和参考记忆错误次数均明显增加(P＜0.05),乌灵菌粉部分逆转了这种戊四唑点燃癫痫诱发的学习能力受损;(3)模型对照组海马、皮层、丘脑和下丘脑组胺含量较空白对照组明显下降(P＜0.05),低剂量乌灵茵粉组海马、高剂量乌灵菌粉组海马、丘脑和下丘脑组胺含量较模型对照组上升(P＜0.05).结论:乌灵茵粉延缓大鼠戊四唑癫痫点燃的形成过程,改善癫痫大鼠的学习能力,增强脑内组胺能神经可能是其作用机制之一.     

定眩颗粒镇静催眠及镇吐作用的实验研究

目的研究定眩颗粒的镇静、催眠、镇吐及对毛细血管通透性的影响.方法采用阈下剂量戊巴比妥钠致小鼠睡眠法、小鼠走动时间和举双肢法,研究受试药的镇静作用;采用硫酸铜和顺铂致家鸽呕吐的模型,观察受试药的镇吐作用;采用小鼠腹腔毛细血管通透性实验,观察受试药对小鼠腹腔毛细血管通透性的影响. 结果定眩颗粒可明显缩短小鼠入睡潜伏期,减少小鼠自发活动次数;延长硫酸铜和顺铂致家鸽呕吐潜伏期,并能减少硫酸铜所致家鸽呕吐次数;降低腹腔毛细血管通透性.结论定眩颗粒具有一定的镇静、催眠及镇吐作用,并能降低毛细血管通透性.     

化疗药物对大鼠卵巢储备功能的影响

目的:探讨化疗药物顺铂对大鼠卵巢储备功能和抗中肾旁管激素(AMH)表达的影响。方法:清洁级成年雌性SD大鼠随机分为对照组(生理盐水)、顺铂低剂量组和顺铂高剂量组,分别进行药物腹腔注射。观察动物的一般情况,称量动物体重,ELISA方法检测血清中AMH水平。结果:(1)与对照组相比,顺铂低剂量和高剂量组动物的一般状态差,体重明显降低(P0.001)。(2)对照组注射后体重比注射前明显升高(P0.001),而另外两组注射后体重均比注射前显著降低(P0.05)。(3)与对照组相比,顺铂低剂量和高剂量组血清AMH水平均显著下降(P0.001);而与顺铂低剂量组相比,高剂量组AMH水平进一步下降(P0.05)。结论:化疗药物顺铂能使大鼠体重和血清AMH水平显著降低,卵巢储备功能下降。AMH是衡量顺铂作用后大鼠卵巢储备功能的重要生物学指标。     

半夏茯苓胶囊防治化疗所致呕吐的临床与实验观察

目的观察半夏茯苓胶囊(Banxia-Fuling Capsule,BFC)对化疗所致恶心、呕吐(chemotherapy induced nauseaand vomiting,CINV)的止吐疗效和副作用。方法38例接受第2周期化疗的恶性肿瘤患者和由顺铂诱导呕吐的家鸽用BFC进行止吐治疗,观察相应的呕吐潜伏期和呕吐发作次数。结果①BFC可以降低患者的恶心、呕吐的程度,可减少患者的呕吐次数并延长呕吐潜伏期,有效率达63.1%,低于阳性对照恩丹西酮组的有效率83.3%,对I0、II0的呕吐效果较好,对III0、IV0呕吐的止吐效果较恩丹西酮差,BFC止吐中除2例出现轻度腹泻外,余无不良反应。②BFC止吐效应与水煎剂相似,均明显延长家鸽的呕吐潜伏期,减少呕吐发作频率和呕吐发生率,呈剂量依赖性。结论BFC防治化学性恶心呕吐(CINV)与传统的汤剂一样安全有效和价廉,无明显的毒副作用,适用于轻中度的呕吐治疗,且较传统的汤剂具有服用和携带方便的优点,患者易于接受;同时对重度的呕吐与恩丹西酮合用可能有协同作用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.