LDL及HDL对内皮细胞血栓调节蛋白mRNA表达的影响

研究ＬＤＬ及ＨＤＬ对血管内皮细胞血栓调节蛋白ｍＲＮＡ表达的影响。利用ＲＴ－ＰＣＲ技术的敏感性，研究分析在各种浓度ＬＤＬ及ＨＤＬ刺激下对牛内皮细胞血栓调节蛋白ｍＲＮＡ表达的影响。结果表明，ＬＤＬ能使ＴＭ－ｍＲＮＡ表达上升，ＬＤＬ和ＨＤＬ浓度的变化对ＴＭ－ｍＲＮＡ的表达均没有影响，而ＨＤＬ与正常对照组相比无明显变化，但ＨＤＬ具有抑制ＬＤＬ促使ＴＭ－ｍＲＮＡ表达的作用。     

不同浓度葡萄糖对大鼠腹膜间皮细胞表达p21WAF1的影响

目的：观察不同浓度的葡萄糖对大鼠腹膜间皮细胞ｐ２１ＷＡＦ１表达的影响。方法：大鼠腹膜间皮细胞在含糖１３．８ｇ／Ｌ和３８．６ｇ／Ｌ的无血清培养基培养２４ｈ后,用流式细胞仪分析细胞周期分布,用ＲＴ－ＰＣＲ法测定ｐ２１ＷＡＦ１的表达水平。结果：经含糖１３．８ｇ／Ｌ和３８．６ｇ／Ｌ培养基培养２４ｈ后腹膜间皮细胞大多出现细胞肥大和停滞于细胞周期的Ｇ１期,以３８．６ｇ／Ｌ组明显。高浓度葡萄糖刺激ｐ２１ＷＡＦ１     

神经干细胞的单层培养及分化过程中ＨＥＳ１和ＭＡＳＨ１的表达变化

摘 要： 【目的】 建立神经干细胞（ＮＳＣ）体外培养方案,使ＮＳＣ的体外可以得到增殖和分化,并检测ＮＳＣ分化过程中ＨＥＳ１和ＭＡＳＨ１的表达变化。【方法】无菌条件下取ＳＤ大鼠胚胎端脑,无血清悬浮培养,形成Ｐ２代神经球后,将神经球消化成单细胞,分为神经球悬浮培养和单层贴壁培养,并对两种不同培养方式下的ＮＳＣ进行干性鉴定和分化能力鉴定。用不同浓度（２０、４０、８０ μｇ／Ｌ）的脑源性神经生长因子（ＢＤＮＦ）诱导ＮＳＣ分化,选出最合适的浓度。用ＢＤＮＦ最佳诱导浓度诱导ＮＳＣ分化,Ｑ－ＰＣＲ检测分化过程中ＨＥＳ１和ＭＡＳＨ１的表达变化。【结果】神经球悬浮培养和单层贴壁培养均可得到的ＮＳＣ;分化３ ｄ后,在不加入任何诱导因素的情况下,单层贴壁培养的ＮＳＣ能更高比例［（１９．５５ ± １．０９）％］地分化为β－ｔｕｂｕｌｉｎ Ⅲ阳性细胞;ＢＤＮＦ ４０ μｇ／Ｌ能诱导出更多的β－ｔｕｂｕｌｉｎ Ⅲ阳性细胞［（３４．１７ ± ０．６０）％］;在分化１、３ 和７ ｄ时,Ｑ－ＯＣＲ结果表明ＭＡＳＨ１在ＢＤＮＦ诱导下组较ＮＳＣ和对照组表达水平明显上升。【结论】单层贴壁培养的神经干细胞更利于分化为神经元,ＢＤＮＦ为４０ μｇ／Ｌ时能诱导出更高比例的神经元,ＢＤＮＦ可以诱导ＭＡＳＨ１在ＮＳＣ分化过程中表达明显上升。     

兔肌乳酸脱氢酶米氏常数〔Km_(NADH)〕的测定

本实验以兔肌乳酸脱氢酶（ＬＤＨ）为受试对象,在ｐＨ７．０,２５℃,１０１３ｋＰａ条件下,采用不同配对的底物浓度（ＮＡＤＨ和丙酮酸）方式,测定了不同时间的光吸收值（Ａ３４０ｎｍ）,并计算出ＮＡＤＨ在Ａ３４０ｎｍ每分钟减少的平均差值（△Ａ３４０ｎｍ／ｍｉｎ）。用二次作图法求出了ＬＤＨ的Ｋｍ（ＮＡＤＨ）值为１０６×１０－５ｍｏｌ／Ｌ,此结果与文献报道接近（１１×１０－５ｍｏｌ／Ｌ）。     

血清HDL—Ch,ApoA1,ApoB和LP（a）测定对预测冠心病的临床意义

目的 探讨血清ＨＤＬ－Ｃｈ、ＡｐｏＡ１、ＡｐｏＢ和ＬＰ（ａ）测定对预测冠心病的临床意义。方法 采用氧化酶直接测定法以及免疫比浊不分别测定总胆固醇（Ｃｈ）、甘油三酯血清高密度脂蛋白（ＨＤＬ－Ｃｈ）和ＬＰ（ａ）,并对冠心病组与健康对照组进行比较。结果 冠心病组与对照组ＨＤＬ－Ｃｈ、ＡｐｏＡ１、ＡｐｏＢ和ＬＰ（ａ）浓度有显差异,当ＨＤＬ－Ｃｈ〈１．０ｍｍｏｌ／Ｌ,ＡｐｏＡ１〈１．０ｇ／Ｌ,ＡｐｏＢ〉０     

从石油中间产品合成表面活性剂AS-4Ⅰ.AS-4水溶液与原油体系的界面活性

以石油中间产品为原料,合成了烃基苯磺酸盐表面活性剂ＡＳ－４。考察了盐浓度,碱浓度和ＡＳ－４ 浓度对ＡＳ－４ 水溶液与原油体系的动态界面张力的影响。结果表明,当Ｎａ２ＳＯ４ 浓度为５～１５ｇ／Ｌ,ＮａＯＨ 浓度为５～１０ｇ／Ｌ,ＡＳ－４ 浓度为０．１～０．５ｇ／Ｌ的条件下,ＡＳ－４ 水溶液与原油体系的动态界面张力具有１０－ ３ｍ Ｎ／ｍ 以下的超低最小值。还研究了ＡＳ－４ 表面活性剂的组成（不同的异构体）对其水溶液与原油体系的动态界面张力的影响。     

纤维蛋白原、极低密度脂蛋白对人脐静脉内皮细胞t-PA和PAI-1mRNA含量的影响

目的　拟建立逆转录－聚合酶链式反应（ＲＴ－ＰＣＲ）半定量法,探讨纤维蛋白原（Ｆｇ）、极低密度脂蛋白（ＶＬＤＬ）对人脐静脉内皮细胞（ＨＵＶＥＣｓ）组织型纤溶酶原激活物（ｔ－ＰＡ）、Ｉ型纤溶酶原灭活剂（ＰＡＩ－ｌ）ｍ ＲＮＡ 表达的影响。　方法　（ｌ）用异硫氰酸胍一步法（ＴＲＩｚｏｌ试剂）提取细胞总ＲＮＡ,用单管ＲＴ－ＰＣＲ 法、三对引物分别逆转录、扩增ｔ－ＰＡ,ＰＡＩ－１ 及内参照三磷酸甘油醛脱氢酶（ＧＡＰＤＨ）的特异性片段,扩增产物经２．０％ 琼脂糖凝胶电泳分离后,用凝胶图像分析仪照相、计算各条带分子量并扫描其光密度值（ｉｎｔｅｇｒａｔｅｄ ｄｅｎｓｉｔｙ ｖａｌｕｅ,ＩＤＶ）,ｔ－ＰＡ、ＰＡＩ－１ ｍ ＲＮＡ的ＩＤＶ 用ＧＡＰＤＨ ｍ ＲＮＡ 的ＩＤＶ 进行校正,其相对表达量用各实验组与对照组比较的倍数表示。（２）以ＥＬＩＳＡ 法测定内皮细胞（ＥＣ）培养上清液（ＣＭ）中ｔ－ＰＡ,ＰＡＩ－１总抗原的含量。（３）ＥＣ与不同浓度Ｆｇ（２,２０,２００,２０００ μｇ／ｍ ｌ）或ＶＬＤＬ（５,１０,２０,３０,４０ μｇ／ｍ ｌ）共同孵育１２ ｈ 或２４ ｈ 后,观察ＥＣｔ－ＰＡ 和ＰＡＩ－１ ｍ ＲＮＡ 水平及ＣＭ 中抗原含量的变化。（４     

疾宁康对阿霉素所致心肌细胞损伤的影响

目的：研究疾宁康醇提物对阿霉素（ＡＤＲ）所致心肌细胞损伤的影响。方法：采用体外心肌细胞培养，观察疾宁康醇提物对心肌细胞博动频率及对心肌细胞培养液中ＬＤＨ活力和ＭＤＡ含量的影响。结果：疾宁康４８００和４８０μｇ／ｍｌ均能明显对抗ＡＤＲ所致受损心肌细胞ＭＤＡ含量和ＬＤＨ活力的升高。４８μｇ／ｍｌ仅能使心肌细胞ＭＤＡ含量下降，对ＬＤＨ活力未见明显影响。疾宁康还能使受损心肌细胞博动频率加快。结论：疾宁康对     

海风藤抑制淀粉样蛋白诱导神经细胞胞浆钙离子升高的研究

为了探索淀粉样蛋白（β－ＡＰ）对神经细胞毒性作用的抑制剂，应用水母发光蛋白导入法研究了海风藤对淀粉样蛋白第２５个氨基酸到３５个氨基酸片断（β－ＡＰ２５－３５）诱导神经细胞胞浆钙离子升高的影响，并应用细胞形态学、细胞存活率、乳酸脱氢酶（ＬＤＨ）释放、细胞集落等指标观察了海风藤对神经细胞的直接作用。结果显示，海风藤在实验浓度内（２．５ｇ／Ｌ～２５ｇ／Ｌ）对神经细胞无毒性作用；海风藤能抑制β－ＡＰ２５－３５诱导的神经细胞胞浆钙离子升高并随海风藤浓度增加而增强。提示海风藤对神经细胞有保护作用，为海风藤防治Ａｌｚｈｅｉｍｅｒｓｄｉｓｅａｓｅ，ＡＤ）提供了依据。     

从石油中间产品合成表面活性剂AS—4：Ⅰ.AS—4水溶 …

以石油中间产品为原料，合成了烃基苯磺酸盐表面活性剂ＡＳ－４。考察了盐浓度，碱浓度和ＡＳ－４浓度对ＡＳ－４水溶液与原油体系的动态界面张力的影响。结果表明，当Ｎａ２ＳＯ４浓度为５－１５ｇ／Ｌ，ＮａＯＨ浓度为５－１０ｇ／Ｌ，ＡＳ－４浓度为０．１－０．５Ｇ     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.