Folin-Ciocalteu比色法测定阜康阿魏叶中总多酚的含量

目的建立阜康阿魏叶中总多酚含量的测定方法。方法以没食子酸为对照品,采用Folin-Ciocal-teu比色法对阜康阿魏叶中总多酚含量进行测定。结果没食子酸对照品在浓度为0.002～0.006g/mL范围内与吸光度呈良好的线性关系(r=0.999 6);阜康阿魏叶中总多酚含量为4.99mg/g,RSD为0.52%;样品溶液在0～10h基本稳定(RSD=1.2%);精密度试验RSD为1.1%;重复性试验RSD为1.1%;平均回收率为101.0%(RSD=1.7%)。结论该法简便快捷,重复性好,为阜康阿魏叶中有效成分的利用提供有利依据。     

RP-HPLC法测定叶下珠中没食子酸、柯里拉京和鞣花酸的含量

目的 建立RP-HPLC法同时测定叶下珠中没食子酸、柯里拉京、鞣花酸含量的方法.方法 采用Diamonsil C18柱(4.6 mm×250 mm,5 μm),以乙腈-0.2%醋酸为流动相,梯度洗脱,检测波长为270 nm,流速1 mL/min,柱温30℃.结果 没食子酸、柯里拉京、鞣花酸的线性范围分别是:0.2～1.0 μg、0.8-4.0 μg、0.4～3.2 μg,平均回收率分别是:100.76%、99.13%、99.77%.结论 测定方法简便、准确、重现性好,可用于叶下珠中没食子酸、柯里拉京、鞣花酸的含量测定.     

小油桐叶中总多酚的含量测定

目的 建立小油桐叶中总多酚的含量测定方法.方法 以没食子酸为对照品,采用Folin-Ciocalteu比色法最适宜条件下对小油桐叶中总多酚含量进行测定.结果 没食子酸对照品在浓度为0.002～0.010 g·L-1范围内与吸光度成良好的线性关系;小油桐叶片中总多酚含量为6.74%,RSD为0.75%;样品溶液在10～90 min内基本稳定(RSD=0.28%);精密度试验RSD为0.23%:重复性实验RSD为1.01%;平均回收率为99.85%.结论 该法灵敏度高,重复性好,简便快捷.     

珠芪复肝颗粒的质量标准初探

目的建立珠芪复肝颗粒的质量控制方法。方法采用薄层色谱(TLC)法对珠芪复肝颗粒方中的黄芪进行定性鉴别,运用高效液相色谱(HPLC)法对该制剂中没食子酸进行含量测定,并对3批珠芪复肝颗粒进行3月的初步稳定性考察。结果珠芪复肝颗粒中黄芪的薄层色谱斑点清晰,且阴性对照无干扰;没食子酸进样量在0.0033~0.0250μg范围内线性关系良好,线性方程为Y=62 059.84X-35 650.77,r=0.9999。平均回收率为100.13%,RSD=1.24%(n=6);各项检验指标均符合规定。结论本试验所建立的检测方法准确、可靠、稳定、重复性好,可用于珠芪复肝颗粒的质量控制,且制剂稳定性良好。     

叶下珠总多酚提取工艺的优选

目的:优选叶下珠总多酚的提取工艺。方法:以总多酚的含量为考察指标,采用L9(34)正交试验法优选叶下珠总多酚的提取工艺。结果:影响因素大小依次是:DBAC,最佳提取工艺条件为A1B2C2D3,即加10倍量的50%乙醇提取3次,每次1h。结论:该提取工艺准确可靠,可行性及重现性良好,有效成分提取率高。     

反相高效液相色谱法测定咽宁含片中没食子酸的含量

目的建立反相高效液相色谱法测定咽宁含片中没食子酸含量的方法。方法采用Agilent ZorbaxSB-C18(4.6×250mm,5μm)色谱柱;以甲醇-水-冰乙酸(18∶81∶1)为流动相;流速为0.8ml.min-1;检测波长272nm,柱温35℃。结果没食子酸的浓度线性范围0.114μg~0.57μg,r=0.99997,平均回收率为99.09%,RSD=2.30%。结论该方法简便灵敏,结果准确,可用于咽宁含片制剂的含量测定。     

HPLC法测定枫蓼肠胃康片中没食子酸的含量

目的建立枫蓼肠胃康片中没食子酸的含量测定方法。方法采用高效液相色谱法,色谱柱:DikmaKromasi1 C18柱;流动相:甲醇-三乙胺-0.5%(体积分数)H3PO4水溶液(体积比5∶0.1∶94.9);流速:1.0mL.min-1;检测波长:273 nm;柱温:室温。结果没食子酸质量浓度在9.82～157.12μg.mL-1范围内线性关系良好(r=0.999 9),平均回收率为97.9%,RSD值为1.49%(n=6)。结论该方法准确、可靠、灵敏度好,可用于枫蓼肠胃康片中没食子酸的含量测定。     

HPLC法测定速效止泻胶囊中没食子酸的含量

目的建立HPLC法测定速效止泻胶囊中没食子酸的含量。方法色谱柱为Phenomnex(luna)C18(4.6mm×250mm,5um),流动相为甲醇-水-N,N-二甲基甲酰胺-冰醋酸(1∶81∶15∶3),流速0.8mL.min-1;柱温为室温;检测波长272 nm。结果没食子酸在4.8~48.0μg.mL-1范围内线性关系良好,平均回收率96.6%,RSD 1.55%(n=6)。结论本法简便、准确、可作速效止泻胶囊的质量控制方法。     

薄层色谱法测定夏枯草中熊果酸的含量

目的 :测定夏枯草中熊果酸的含量。方法 :采用双波长薄层扫描法。以环己烷∶氯仿∶醋酸乙酯∶冰醋酸 ( 2 0∶5∶8∶0 .5)为展开剂 ,选择λS=530nm ,λR=70 0nm作为熊果酸含量测定的扫描波长 ,对夏枯草中熊果酸的含量进行了测定 ,并对方法学进行了考察。结果 :加样回收测定熊果酸的平均回收率为 10 0 .32 % (RDS =2 .14 % )。结论 :此法重现性、回收率良好 ,专属性较强 ,是中药制剂中夏枯草质量控制较为理想的指标。     

HPLC法测定地锦草中游离和总没食子酸含量

目的：建立HPLC法测定地锦草中游离没食子酸和总没食子酸的含量。方法：采用Agela Odyssil C18（4.6 mm×250 mm,5 μm）色谱柱;流动相为甲醇-0.5%磷酸溶液（8∶92）;检测波长270 nm;流速1.0 mL·min-1;柱温30 ℃。结果：没食子酸在0.034 0~1.700 2 μg范围内呈良好线性关系（r=1.000 0）,游离没食子酸和总没食子酸平均回收率分别为99.63%和99.34%。结论：该方法操作简便准确,灵敏度高,重复性好,可用于地锦草中没食子酸的质量控制。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.