白介素-6在川崎病患儿血清中的表达及变化

目的 探究白介素-6(IL-6)在川崎病患儿血清中的表达及变化。方法 选取2020年1月至2022年1月江西省九江市妇幼保健院住院和门诊收治的60例川崎病患儿作为观察组,并选取同期九江市妇幼保健院的60例健康体检患儿作为对照组,检测两组患儿血清IL-6水平,分析观察组患儿急性期与恢复期IL-6表达变化情况,根据患儿有无冠脉损伤分为冠脉损伤组与非冠脉损伤组,根据患儿有无并发症分为并发症组与无并发症组,比较各组IL-6表达情况,分析IL-6对川崎病的诊断价值。结果 观察组IL-6水平高于对照组,差异有统计学意义(P<0.05)。急性期IL-6水平高于恢复期,差异有统计学意义(P<0.05)。冠脉损伤组IL-6水平高于非冠脉损伤组,差异有统计学意义(P<0.05)。并发症组IL-6水平高于非并发症组,差异有统计学意义(P<0.05)。IL-6诊断小儿川崎病敏感度、特异度分别为93.63%、89.45%。川崎病患儿冠状动脉最大内径与急性期、恢复期IL-6水平呈正相关(r=0.426、0.616,P<0.05)。结论 IL-6在川崎病患儿血清中呈高表达,与患儿疾病状...     

病毒性心肌炎患儿血清单核细胞趋化蛋白-1、白介素-6水平的变化

目的探讨单核细胞趋化蛋白-1(MCP-1)、白介素-6(IL-6)在病毒性心肌炎(VMC)发病中的作用。方法采用酶联免疫吸附法(ELISA)检测40例VMC患儿(其中急性期24例,恢复期16例)和20名健康儿童(对照组)血清MCP-1、IL-6水平,用微粒子化学发光免疫分析法测定心肌肌钙蛋白I(cT-nI)水平,并对MCP-1、IL-6与cTnI的相关性进行分析。结果 VMC急性期血清MCP-1、IL-6、cTnI水平显著高于恢复期及对照组(P<0.01),且MIF、IL-6与cTnI呈正相关(P<0.05)。结论 MCP-1、IL-6可能参与VMC发病过程,检测VMC患儿MCP-1、IL-6含量有助于VMC的诊断、判断病情和预后,并为临床治疗开辟新途径。     

脑梗死患者血清TNF-α、IL-6和IL-8水平变化的临床意义分析

目的 分析脑梗死患者急性期与恢复期血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和白细胞介素-8(IL-8)水平变化的临床意义。方法 65例脑梗死患者和同期30例健康体检者(对照组),采用双抗体夹心酶联免疫吸附法(ELISA)检测脑梗死患者急性期、恢复期和对照组的血清TNF-α、IL-6和IL-8水平,比较三组间差异。结果 脑梗死急性期患者血清TNF-α、IL-6和IL-8水平均明显高于恢复期和对照组,三组间差异有统计学意义(F值分别为4.654、5.382和3.386,均P<0.05)。结论 脑梗死患者急性期血清TNF-α、IL-6和IL-8水平明显升高,随着病情的恢复呈逐渐下降趋势,有助于判断病情的预后。     

脑梗死患者血清TNF-α、IL-6和IL-8水平变化的临床意义分析

目的 分析脑梗死患者急性期与恢复期血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和白细胞介素-8(IL-8)水平变化的临床意义。方法 65例脑梗死患者和同期30例健康体检者(对照组),采用双抗体夹心酶联免疫吸附法(ELISA)检测脑梗死患者急性期、恢复期和对照组的血清TNF-α、IL-6和IL-8水平,比较三组间差异。结果 脑梗死急性期患者血清TNF-α、IL-6和IL-8水平均明显高于恢复期和对照组,三组间差异有统计学意义(F值分别为4.654、5.382和3.386,均P<0.05)。结论 脑梗死患者急性期血清TNF-α、IL-6和IL-8水平明显升高,随着病情的恢复呈逐渐下降趋势,有助于判断病情的预后。     

脑梗死患者血清TNF-α、IL-6和IL-8水平变化的临床意义分析

目的 分析脑梗死患者急性期与恢复期血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和白细胞介素-8(IL-8)水平变化的临床意义。方法 65例脑梗死患者和同期30例健康体检者(对照组),采用双抗体夹心酶联免疫吸附法(ELISA)检测脑梗死患者急性期、恢复期和对照组的血清TNF-α、IL-6和IL-8水平,比较三组间差异。结果 脑梗死急性期患者血清TNF-α、IL-6和IL-8水平均明显高于恢复期和对照组,三组间差异有统计学意义(F值分别为4.654、5.382和3.386,均P<0.05)。结论 脑梗死患者急性期血清TNF-α、IL-6和IL-8水平明显升高,随着病情的恢复呈逐渐下降趋势,有助于判断病情的预后。     

新生儿细菌感染与降钙素原、白介素-6水平的相关性研究

目的探讨降钙素原、白介素-6(IL-6)水平在新生儿细菌感染早期诊断及判断预后中的作用。方法随机选择细菌感染急性期新生儿30例作为研究对象(设为研究组),同时选取健康新生儿30例作为健康对照组,分别检测研究组感染急性期、感染恢复期以及健康对照组新生儿血清中降钙素原、白介素-6水平,并进行统计学分析。结果研究组感染急性期血清降钙素原及白介素-6水平显著高于感染恢复期及健康对照组,差异有统计学意义(P<0.05);研究组感染恢复期和健康对照组血清降钙素原及白介素-6水平比较,差异无统计学意义(P>0.05)。结论新生儿细菌感染急性期,PCT和IL-6均显著增高,随着病情好转后两者的水平逐渐下降。PCT和IL-6可作为早期诊断新生儿感染的辅助方法,对早期发现新生儿感染性疾病及判断患儿预后有着重要意义。     

0~3岁反复上呼吸道感染患儿血清细胞因子IL-6、IL-10和TNF-α的水平及意义

目的 测定0~3岁反复上呼吸道感染(rURTIs)患儿血清细胞因子IL-6、IL-10、TNF-α水平变化并探讨其意义.方法 选取2013年5月至2014年4月治疗的0~3岁rURTIs患儿90例,其中0~1岁30例,1~2岁30例,2~3岁30例,将其列为观察组,另选取0~3岁健康儿童90例为对照组,其中0~1岁30例,1~2岁30例,2~3岁30例,检测血清细胞因子IL-6、IL-10、TNF-α水平,比较其差异.结果 0~3岁组rURTIs患儿血清IL-6水平明显高于对照组(P<0寣.01);0~1岁、2~3岁组rURTIs患儿血清IL-10水平与对照组差异无统计学意义(P>0.05),1~2岁、0~3岁组rURTIs患儿血清IL-10水平明显低于对照组(P<0.01,P<0.05);0~1岁、1~2岁、2~3岁、0~3岁反复上感组患儿血清TNF-α水平明显高于对照组(P<0.05或<0.01).结论 0~3岁rURTIs患儿血清细胞因子IL-6、IL-10、TNF-α有明显变化,提示细胞因子参与了rURTIs的发病,幼儿细胞免疫功能下降对rURTIs的发生、发展具有重要意义.     

狼疮性肾炎患者血清白介素-6、白介素-10水平及临床意义

目的探讨狼疮性肾炎患者血清白介素-6、白介素-10水平及临床意义。方法选择2014年1月~2016年6月在我院诊断为狼疮性肾炎患者40例为实验组,其中20例患者处于活动期,缓解期患者20例,另选择同期健康体检志愿者20例为对照组。检测三组血清IL-6及IL-10水平。结果实验组活动期IL-6及IL-10水平显著高于缓解期及对照组,差异有统计学意义(P<0.05)。缓解期IL-6及IL-10水平显著高于对照组,差异有统计学意义(P<0.05)。实验组活动期Trge水平显著低于缓解期以及对照组,差异有统计学意义(P<0.05);实验组活动期CD19+CD69+显著高于缓解期以及对照组,缓解期显著高于对照组,差异有统计学意义(P<0.05)。结论狼疮性肾炎活动期患者血清IL-6、IL-10以及B淋巴细胞水平显著升高,Treg水平下降,其可以作为临床判断狼疮性肾炎活动程度的相关指标。     

类风湿关节炎患者血清白介素-1β白介素-6 肿瘤死亡因子-α的水平测定及其临床意义

目的通过测定类风湿关节炎（RA）患者和正常对照组血清白介素-1β（IL-1β）、白介素-6（IL-6）、肿瘤死亡因子-α（TNF-α）的水平,探讨这些细胞因子在RA发病机制中的作用。方法收集RA患者20例随机分为两组和健康体检者10例为正常对照组,均采用酶联免疫吸附测定法（ELISA）检测血清IL-1β、IL-6和TNF-α的水平。结果MTX＋TGP＋LEF组与MTX组比较,前者治疗效果明显优于后者。治疗前RA患者血清中IL-1β、IL-6和TNF-α的水平均高于正常对照组（P〈0.01）,具有显著性统计学意义。两组RA患者治疗后血清中IL-1β、IL-6和TNF-α的水平与治疗前比较（P〈0.05）,具有显著性统计学意义。但与正常对照组比较除TNF-α外,IL-1β、IL-6均为P〉0.05,无统计学意义。结论IL-1β、IL-6和TNF-α在RA患者发病、发展中起着重要作用,定期检测其水平并采取对应治疗,对降低RA患者软骨破坏,提高疗效有着重要意义。     

细胞因子谱、C-反应蛋白及降钙素原在小儿重症支原体肺炎诊治中的临床意义

目的 探讨小儿肺炎急性期血清细胞因子白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、白细胞介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)、C-反应蛋白(CRP)、降钙素原(PCT)的浓度水平变化在小儿重症支原体肺炎诊治中临床意义.方法 选取肺炎患儿127例,将其中64例支原体肺炎(MPP)作为研究组,63例非支原体肺炎(NMPP)患儿作为对照组;并将研究组分为重症组和轻症组,同时又可将重症组MPP分为急性期和恢复期.采用酶联免疫吸附实验法(ELISA)检测各组急性期血清CRP、PCT、IL-1β、IL-6、IL-8、IL-10、TNF-α的浓度变化及重症MPP组恢复期各炎症指标浓度水平.结果 研究组和对照组患儿血清IL-1β浓度水平变化无统计学意义(P>0.05).重症组患儿急性期血清IL-6、IL-8、TNF-α、CRP、PCT的浓度水平均高于轻症组、对照组和重症组恢复期比较,均差异有统计学意义(P<0.05),而血清IL-10在重症组、轻症组和对照组的浓度水平比较,差异无统计学意义(P>0.05).ROC曲线分析血清IL-6检测在诊断MPP上特异性较高.结论 血清IL-6、IL-8、TNF-α、CRP、PCT等炎症指标对早期诊治重症MPP有重要意义.     

Impact of interleukin-1 and interleukin-6 in murine primary schistosomiasis

BACKGROUND: Immunization with schistosome antigens invariably elicits a plethora of cytokines and, hence, it is reasonable to assume that these cytokines influence host responses to challenge lung-stage larvae and, consequently, the adult worm burden, and may be responsible for the erratic data generally observed in protection studies against schistosome infection. METHODS: Schistosoma mansoni-infected mice were administered with recombinant interleukin (IL)-1beta or IL-6 to evaluate the impact of cytokines in host responses to lung-stage schistosomula, and subsequent effects on adult worm parameters. Plasma lipid levels were assayed by colorimetric enzymatic tests and antibody responses by ELISA. Cytokine profile in peripheral blood mononuclear cells was evaluated by RT-PCR. RESULTS: S. mansoni infection elicited, at the time of parasite residency in the lung, significant increase in free fatty acids (FA) and decrease in cholesterol plasma levels in C57BL/6 and CD1 mice, and stimulation of mRNA expression for cytokines of T helper type (Th) 2 in BALB/c, Th1 in C57BL/6, and Th1/Th2 in CD1 mice. However, no specific antibody production was evident in any mouse strain. In BALB/c mice, exogenous IL-1beta-related plasma free FA level significant increase, stimulation of expression of IL-1 and IL-12 mRNA, and considerable increase in percent of specific antibody-producing mice were associated with significant reduction in adult worm burden and egg load. In contrast, exogenous IL-1beta elicited decrease in free FA plasma levels, and down-regulation of cytokines' mRNA expression in C57BL/6 and CD1 mice, changes associated with aggravation of the worm burden. Likewise, exogenous IL-6 failed to stimulate increase in plasma free FA levels or percent of antibody-producing mice except in BALB/c mice, effects that were protective for the host in BALB/c and for the parasite in C57BL/6 and CD1 mice. CONCLUSION: These findings were discussed in relation to the erratic data of protection experiments with schistosome subunit antigens in different mouse strains.     

白细胞介素-1、8在门脉高压症中的作用

目的：探讨白细胞介素－1、8在门脉高压症中的作用。方法：放免分析法测定白细胞介素－1、8含量。结果：白细胞介素－1、8与一氧化氮合成酶（NOS）、谷丙转氨酶（ALT）、谷草转氨酶（AST）有高度相关性。结论：白细胞介素－1、8参与高动力循环综合征（HCS）并与肝功能的损害有一定的关系。     

黄芪对寻常型银屑病患者IL-6、IL-8及IL-10血浆水平的影响

目的研究黄芪对进展期寻常型银屑病患者血浆IL-6、IL-8与IL-10水平的影响。方法采用放射免疫法检测57例进展期寻常型银屑病患者用黄芪治疗前后血浆IL-6、IL-8与IL-10水平的变化。结果黄芪能降低血浆中过高的IL-6、IL-8水平(P<0.01),升高IL-10水平(P<0.05)。结论黄芪能通过调节细胞因子与淋巴细胞功能而治疗银屑病。     

双抗体夹心法测定注射用重组人白细胞介素-2的含量

目的:建立用双抗体夹心法(ELISA 法)测定注射用重组人白细胞介素-2的含量。方法:采用酶标仪在450 nm 处测量白细胞介素-2复合物的 A_(450nm)值,并计算重组人白细胞介素-2冻干粉的百分含量。结果:白细胞介素-2浓度在31.25～500 pg·mL~(-1)范围内线性关系良好(r=0.9999);低、中、高3种浓度的回收率分别为97.3%,98.4%,101.4%,RSD 均小于5.0%。质量控制符合要求。精密度在误差许可范围内。注射用重组人白细胞介素-2的含量为49.55μg·mg~(-1)。结论:方法准确、可靠,注射剂辅料对测定无干扰,可用于注射用重组人白细胞介素-2的含量测定。     

白介素-4、白介素-5在鼻息肉及鼻息肉病组织中的表达

目的探讨白介素-4（IL-4）、白介素-5（IL-5）和嗜酸性粒细胞（EOS）在人类鼻息肉及鼻息肉病中的表达情况。方法采用免疫组织化学SP方法检测30例鼻息肉患者（鼻息肉组）、25例鼻息肉病患者（鼻息肉病组）的息肉组织及10例中鼻甲黏膜（对照组）中IL-4、IL-5的表达情况,同时以HE染色计数各组织中EOS的浸润程度。结果IL-4、IL-5在对照组、鼻息肉组及鼻息肉病组间呈递增性表达（P〈0.01）,且后2组差异均有统计学意义（P〈0.01）。结论炎性细胞因子IL-4和IL-5在鼻息肉的发病中起重要作用。     

Fluoride-induced interleukin-6 and interleukin-8 synthesis in human epithelial lung cells

Exposure to fluorides has been associated with asthmatic symptoms among workers in the aluminium industry. In a recent experimental study hydrogen fluoride (HF) was found to induce a weak inflammatory response in humans. In the present study the potential of sodium fluoride (NaF) and HF to induce cytokine response was examined and how these responses are modulated by Al3+ in a human epithelial lung cell line (A549). Dose-response experiments showed a maximal release of IL-6 and IL-8 at a concentration of 5 mM NaF 24 h after addition. The responses to HF were of a similar magnitude as for NaF. Time-course experiments showed a NaF-induced IL-6 response at 5 h, whereas an IL-8 response was observed after 10 h. Cycloheximide treatment completely abolished the NaF-induced cytokine responses. A marked increase in the mRNA level for IL-6 was observed already 2 h after exposure to 5 mM NaF, and presumably is a prerequisite for the subsequent increase of IL-6. The fluoride-induced effects on IL-6 and IL-8 release were strongly reduced by pretreatment with deferoxamine (an Al3+-chelator), and enhanced by addition of Al3+. This indicates that an AlF4-- complex, a known activator of GTP-binding proteins, is involved in fluoride-induced IL-6 and IL-8 responses in A549 cells.     

依达拉奉对单肺通气患者白细胞介素6和10的影响

目的观察依达拉奉对单肺通气患者围术期白细胞介素6(IL-6)和白细胞介素10(IL-10)表达的影响。方法择期拟行肺叶切除术肺癌患者24例,ASAⅠ级或Ⅱ级,年龄48～67岁,随机分为2组,对照组和依达拉奉组各12例。2组均静脉注射咪达唑仑0.03mg·kg~(-1)、芬太尼3μg·kg~(-1),吸入8%七氟醚麻醉诱导。麻醉诱导后,依达拉奉组给予依达拉奉0.5mg·kg~(1-)加入氯化钠注射液100mL,静脉滴注;对照组给予等量氯化钠注射液。2组麻醉维持用药相同,均在麻醉后切皮前(T_1)、膨肺后60min(T_2)、术后1h(T_3)测定血浆IL-6、IL-10浓度及其mRNA表达。结果与T_1比较,2组血浆IL-6、IL-10浓度和IL-6/IL-10以及IL-6mRNA、IL-10mRNA和IL-6mRNA/IL-10 mRNA于T_2、T_3均明显上升(P<0.05)。与对照组比较,依达拉奉组血浆IL-6浓度、IL-6mRNA、IL-6/IL-10和IL-6mRNA/IL-10mRNA于T_2、T_3均显著降低(P<0.05)。结论依达拉奉可有效抑制单肺通气患者IL-6的生成和释放,有助于维持IL-6/IL-10相对稳定。     

Effects of phosphodiesterase inhibitors on interleukin-4 and interleukin-13 generation from human basophils

The aim of the present study was to determine whether inhibition of cyclic nucleotide phosphodiesterase (PDE) modulates the stimulated generation of the cytokines, interleukin-4 (IL-4) and IL-13, from human basophils. This was addressed by evaluating the effects of both nonselective and selective inhibitors of PDEs on the generation of cytokines from basophils. The nonselective PDE inhibitors, isobutyl-methylxanthine (IBMX) and theophylline, attenuated the IgE-mediated generation of IL-4 and IL-13 and, also, the release of histamine from basophils. The effects of the isoform-selective inhibitors, 8-methoxymethyl-IBMX (PDE 1 inhibitor), siguazodan (PDE3 inhibitor), rolipram (PDE4 inhibitor), denbufylline (PDE4 inhibitor), Org 30029 (mixed PDE3 and 4 inhibitor) and zaprinast (PDE5 inhibitor), were studied. Of these selective compounds, only rolipram, denbufylline and Org 30029 inhibited the IgE-dependent generation of IL-4, IL-13 and histamine from basophils to a statistically significant (P<0.05) degree. The effects of isoform-selective inhibitors on basophils activated by IL-3 were evaluated. The IL-3-induced generation of IL-4, IL-13 and histamine was inhibited to a statistically significant (P<0.05) extent, only by compounds that act as inhibitors of PDE4. These data suggest that inhibition of PDE4 can regulate the generation of cytokines from human basophils.