高效液相色谱法测定妇炎灵泡腾片中蛇床子素的含量

目的 :建立妇炎灵泡腾片中蛇床子素含量测定方法。方法 :采用高效液相色谱法测定。色谱柱为NOVA PAKC1 8柱 (3 .9mm× 1 50mm ,4μm) ,流动相为甲醇 水 (75∶2 5) ,流速 1mL/min ,检测波长 32 2nm ,柱温 30℃。结果 :回收率为 97.5 % (n =5) ,重现性RSD =1 .41 % (n =5)。结论 :该方法简便、准确 ,可用于妇炎灵泡腾片的质量控制     

Determination of Matrine in Baicaofuyanqing Suppository with High Performance Liquid Chromatography

目的:建立用高效液相色谱法(HPLC)测定百草妇炎清栓中苦参碱含量的方法.方法:采用HPLC法,色谱柱为Agilent ZORBAX-NH2(4.6m×250mm,5μm),流动相为乙睛-无水乙醇-1.5％磷酸(80:10:10),流速1.0ml/min,检测波长220nm,柱温35℃.结果:苦参碱在0.0656～0.8528μg与峰面积成线性关系(r=0.9999),该制剂中苦参碱的平均回收率为101.01％,RSD为1.41％(n=6).结论:该高效液相色谱法简便易行、准确、耐用,可用于百草妇炎清栓中苦参碱的含量测定.     

高效液相色谱法测定百草妇炎清栓中苦参碱含量

目的:建立用高效液相色谱法(HPLC)测定百草妇炎清栓中苦参碱含量的方法。方法:采用HPLC法,色谱柱为Agilent ZORBAX-NH2(4.6 mm×250 mm,5μm),流动相为乙睛-无水乙醇-1.5%磷酸(80∶10∶10),流速1.0 ml/min,检测波长220 nm,柱温35℃。结果:苦参碱在0.065 6～0.852 8μg与峰面积成线性关系(r=0.999 9),该制剂中苦参碱的平均回收率为101.01%,RSD为1.41%(n=6)。结论:该高效液相色谱法简便易行、准确、耐用,可用于百草妇炎清栓中苦参碱的含量测定。     

坤乐泡腾片的质量控制研究

目的:对坤乐泡腾片进行质量控制研究.方法:采用簿层色谱对蛇床子和苦参进行定性,采用酸碱滴定法测定苦参总碱的含量,采用高效液相色谱法测蛇床子素的含量,以十八烷基硅烷键合硅胶为填充剂:以甲醇-水(75:25)为流动性:流速1 ml/min,检测波长为322nm,温度25℃.理论塔板数按蛇床子素峰机算应不低于3000.结果:苦参总碱的含量为5%.蛇床子素含量为1.58%,在0.52 μg～3.12 μg范围内呈线性关系,平均加样回收率为99.02%,RSD=2.64.结论:本法操作简单,结果准确,重复性好,可用于蛇床子和苦参泡腾片的质量检测.     

高效液相色谱法测定复方苦参洗剂中苦参碱含量

目的：建立复方苦参洗剂中苦参碱的含量测定方法。方法：反相高效液相色谱法,采用Diamonsil不锈钢C18色谱柱（4.6 mm×150 mm,5μm）,以甲醇-水-三乙胺（45∶55∶0.05）为流动相,紫外-可见检测器测定波长为220 nm,流速为0.6 mL/min。结果：苦参碱在12.60~126.00 mg.L-1与峰面积之间线性关系良好,r=0.9995,回收率为99.65%,RSD为2.3%。结论：本方法用于复方苦参洗剂中苦参碱含量的测定,简单、快速、准确。     

高效液相色谱法测定祛瘀散结胶囊中人参皂苷Rg1的含量

[目的]建立测定祛瘀散结胶囊中人参皂苷Rg 1含量的方法.[方法]采用高效液相色谱法,所用色谱柱为Thermo色谱柱(4.6 mm×250.0 mm,5μm),流动相为乙腈-水(19∶81),流速为1 mL/min,检测波长为203 nm.[结果]人参皂苷Rg 1的进样量在0.325~5.200μg范围内时与峰面积积分值呈良好的线性关系,Y=369.089X+15.813,r=0.999 8(n=5),加样平均回收率为99.77%,RSD为1.57%.[结论]采用高效液相色谱法测定祛瘀散结胶囊中人参皂苷Rg 1含量的方法具有简便、快速、灵敏及重现性好等优点,可作为祛瘀散结胶囊质量控制方法.     

高效液相色谱法测定复方苦参子洗液中蛇床子素含量

目的建立测定复方苦参子洗液中蛇床子含量的高效液相色谱法(HPLC).方法 色谱柱:Turner C18柱;流动相:甲醇-水-磷酸(65:35:0.1)流速:1.0 mL?min-1;柱温:30℃;检测波长:322 nm;进样量:20μL.结果 蛇床子素进样量在0.42~4.2ug范围内与峰面积线性关系良好(r=0.9996),平均回收率为98.20%,RSD=1.68%(n=6).结论 用HPLC测定蛇床子素含量,方法简单、准确,重复性好.     

高效液相色谱法测定八正泡腾片中大黄素的含量

目的 建立八正泡腾片质量标准的含量测定项目.方法 采用高效液相色谱法测定八正泡腾片中大黄素的含量,色谱柱为 Dikma C18(250mm×4.6mm,5μm),流动相为甲醇-0.1%磷酸溶液(85:15),检测波长为290nm,流速为1mL·min -1,柱温为40℃.结果 大黄素的线性范围为0.04992～0.3328μg(r=0.9999),平均回收率为100.88%(n=6,RSD=2.02%).结论 高效液相色谱法简便、准确,可以有效控制八正泡腾片的质量.     

牛黄降压片中冰片含量及樟脑残留量的测定

目的：建立牛黄降压片中冰片含量及樟脑残留量的测定方法。方法：气相色谱法,采用色谱柱19091N-213和INNOWAX石英毛细管色谱柱（30 m×0.32 mm×0.5μm）,柱温130℃,FID为检测器,无水乙醇为提取溶剂。结果：冰片质量浓度在0.0975～3.8984 mg/mL 范围内与峰面积呈良好的线性关系（r=0.9999）,平均加样回收率（n=6）为101.0%,相对标准偏差（relative standard deviation,RSD）为0.71%;樟脑浓度在1.041～20.82μg/μL范围内与峰面积呈现良好的线性关系（r=0.9995）,平均加样回收率（n=6）为100.4%, RSD为1.4%。结论：气相色谱法简便、灵敏、专属性强,适用于牛黄降压片的质量控制。     

反相高效液相色谱法测定朝鲜大黄中大黄酸的含量

[目的]测定朝鲜大黄中大黄酸的含量.[方法]采用反相高效液相色谱法,色谱柱为VP-ODS色谱柱(4.6 mm×150 mm,5μm),以1 mL/L甲醇-磷酸(85∶15)为流动相,流动速度为1.0 mL/min,检测波长为254 nm.[结果]大黄酸进样量在0.08~0.80μg范围内,其峰面积积分值与质量浓度之间呈良好的线性关系(r=0.999 8),平均回收率为99.58%(n=6),RSD为2.85%.药用大黄及朝鲜大黄中大黄酸含量分别为2.562 8,12.088 8 mg/g.[结论]反相高效液相色谱法测定朝鲜大黄中大黄酸含量的方法简便、准确,可用于朝鲜大黄中大黄酸含量的测定;朝鲜大黄中大黄酸含量高于药用大黄.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.