脑外科手术中机器人的应用

临床应用国产机器人系统辅助完成脑外科立体定向手术。方法：机器人系统主要由影像引导装置，三维定位软件和智能机械臂等部分组成。手术时，机器人系统通过识别贴附于患者头部的标志点，建立相应的三维坐标体系；智能机械臂按照手术规划自动地锁定，为术者准确导航并提供操作平台。     

机器人系统辅助脑立体定向手术

目的：作者应用国产ＣＲＡＳ－ＨＢ型机器人系统（Ｃｏｍｐｕｅｒ ａｎｄ ｒｏｂｏｔ ａｓｓｉｓｔｅｄ ｓｕｒｇｅｒｙ，ｔｙｐｅ ＢＨ１）辅助完成脑立体定向手术。方法 机器人系统主要是由ＰＵＭＡ２６０硬件平台、图像引导定位软件、放射性药物注入控制等模拟组成。结果：利用该系统成功地辅助完成立体定向脑肿瘤内放疗、脑内血肿排空、脑内病变活检等手术，结论：该机器人设计结构合理，操作集成实用性强、定位精确且操作     

HDV/HBV体外感染原代培养人胎肝细胞的实验研究

目的：建立ＨＤＶ／ＨＢＶ感染人胎肝细胞体外培养系统。方法：利用ＨＤＶ／ＨＢＶ阳性血清同时感染体外２的人胎肝细胞;应用ＥＬＩＳＡ、免疫组化法、原位杂交法和斑点法检测上清液和细胞中ＨＢｓＡｇ、ＨＤＡｇ、ＨＢＶＤＮＡ和ＨＤＶｃＤＮＡ。结果：上清液和细胞中ＨＢｓＡｇ、ＨＤＡｇ、ＨＢＶＤＮＡ和ＨＤＶｃＤＮＡ在感染后第２天至第１６天均可测出,其中上清液中ＨＢｓＡｇ、ＨＤＡｇ以感染后第４天至第１２天达高峰,结论     

标本溶血对乙肝表面抗原检测的影响

在ＨＢｓＡｇ的林测中，常常遇到有溶血的标本，笔者用机械溶血的方法北照检测了溶血和非溶血标本的ＨＢｓＡｇ，现将结果报告如下．１ 临床资料 样本：３０例血液标本取自本院就诊病人。抽取静脉血３ｍｌ，凝固后离心分离部分血清标本；再用机械法造成溶血，离心后取其溶血血清．另在ＨＢｓＡｇ常砚检测中留取１０份ＯＤ值附近的血清及机械溶血后离心取其溶血血清． 试剂：ＨＢｓＡｇ试剂盒由深圳月弯湾生物工程公司提供，批弓：９９０８０１１，有效期内使用，操作按说明书． 仪器：ＤＥＮＬＥＹ　ＤＲＡＧＯＮ　ＮＫ２酶标仪．２ 结 果 …     

HBV_2患者血清HBsAg的氨基酸组成分析

将２例ＨＢＶ＿２患者血清ＨＢｓＡｇ纯化，分析了ＨＢＶ＿２的ＨＢｓＡｇ氨基酸组成，并与分子量为６３０１９８道尔顿ＨＢＶ＿１的ＨＢｓＡｇ三种多肽氨基醚组成比较。实验发现，ＨＢＶ＿２与ＨＢＶ＿１的ＨＢｓＡｇ的氨基醚组成有较大区别，且区别指数均在１３以上。提示ＨＢＶ＿１和ＨＢＶ＿２的ＨＢｓＡｇ在一级结构上存在较大差异；ＨＢＶ＿２可能系一新的乙型肝炎病毒，也可能系由于ＨＢＶ＿１Ｓ区核苷酸突变所致的变异株。     

抗人卵巢癌单克隆抗体COC183-B2重链可变区基因的体外扩增、克隆及序列分析

目的：分离抗人卵巢癌单克隆抗体ＣＯＣ１８３Ｂ２重链可变区（ＶＨ）基因并测定其序列。方法：用反转录ＰＣＲ扩增抗人卵巢癌单抗ＣＯＣ１８３Ｂ２ＶＨ基因，将其克隆入ＰＵＣ１９载体，重组子用Ｓａｎｇｅｒ’ｓ双脱氧链终止法测定序列，将序列与ＧｅｎｅＢａｎｋ及已发表的抗体序列比较。结果：ＶＨ基因全长３５４ｂｐ，属鼠免疫球蛋白重链混杂亚类（ｓｕｂｇｒｏｕｐｍｉｓｃｅｌａｎｅｏｕｓ），由种系基因的ＶＨ，Ｄｓｐ２．５及ＭＵＳＪＨ４重排而来。该ＶＨ基因序列已被ＧｅｎｅＢａｎｋ收录（ａｃｃｅｓｓｉｏｎＮｏＡＦ０４５０２３）。结论：该ＶＨ基因为抗人卵巢癌单抗ＣＯＣ１８３Ｂ２功能性重链可变区基因。     

HBV感染者血中大颗粒淋巴细胞的测定

分５组测定了乙型肝炎病毒（ＨＢＶ）感染者血中大颗粒淋巴细胞（ＬＧＬ）的含量，每组各３０例。其中（１）组：乙型肝炎表面抗原（ＨＢｓＡｇ）、ｅ抗原（ＨＢｅＡｇ）和核心抗体（ＨＢｃＡｂ）均为阳性；（２）组：为ＨＢｓＡｇ和ＨＢｅＡｇ阳性者；（３）组：为ＨＢｓＡｇ阳性、抗－ＨＢｅ和抗－ＨＢｃ阳性者；（４）组：为抗－ＨＢｓ阳性者；（５）组：为抗－ＨＢｓ和抗－ＨＢｅ阳性者。并且与对照组进行比较。结果表明，（１＿     

乙型肝炎疫苗8741—1对新生儿的免疫效果

目的：观察乙肝疫苗８７４１－１对新生儿的免疫效果。方法：对３４例ＨＢｓＡｇ阳性孕妇所生子女进行该疫苗的预防注射，注射后的不同时期抽血检查血清抗－ＨＢｓ、ＨＢｓＡｇ、抗－ＨＢｃ的滴度。结果：抗－ＨＢｓ阳性率为８２４％～９６９％，有效保护率为７３５％～９３８％，ＨＢｓＡｇ阳性率为２９４％～８８２％。结论：乙肝疫苗８７４１－１对新生儿具有良好的免疫结果。     

白马骨根水提取物的体外抗乙肝病毒作用

采用ＨＢＶ ＤＮＡ转染细胞（２．２．１５细胞）为靶细胞，用Ａｒａ－Ａｍｐ为阳性对照药，观察在不同浓度下白马骨根水提取物对２．２．１５细胞表达ＨＢｓＡｇ、ＨＢｅＡｇ的影响。结果显示：白马骨根水提取物在１２．５ｍｇ￣１００ｍｇ／ｍｌ的浓度范围内，对２．２．１５细胞分泌ＨＢｓＡｇ、ＨＢｅＡｇ有抑制作用。     

精神科住院患者乙肝病毒感染状况调查

目的：探讨精神科住院患者乙肝病毒（ＨＢＶ）感染状况,为防治ＨＢＶ 的院内感染提供参考依据。方法：采用酶联免疫吸附试验（ＥＬＩＳＡ）对１２９５ 例住院精神病患者进行血清ＨＢＶ标志物检测。结果：血清ＨＢｓＡｇ、抗－ＨＢｓ、ＨＢｅＡｇ、抗－ＨＢｅ、抗－ＨＢｃ 五项ＨＢＶ 标志物阳性率分别为８．７３％ 、３１．０４％ 、２．７％ 、２１．７８％ 、１５．２９％ ,ＨＢＶ 总感染率为４３．６３％ ;５６５ 例阳性标本检出ＨＢＶ感染模式１５ 种,ＨＢｓＡｇ（＋ ）、抗－ＨＢｓ（－ ）、ＨＢｅＡｇ（－ ）、抗－ＨＢｅ（＋ ）、抗－ＨＢｃ（＋ ）（小三阳）占１２．２％ ,ＨＢｓＡｇ（＋ ）、抗－ＨＢｓ（－ ）、ＨＢｅＡｇ（＋ ）、抗－ＨＢｅ（－ ）、抗－ＨＢｃ（＋ ）（大三阳）占５．８４％ 。结论：精神科住院患者中存在ＨＢＶ 传染源,病区应加强监测、隔离、消毒等综合性措施,预防住院患者ＨＢＶ 的院内感染     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.