Alteration of lipid peroxidation and antioxidant enzymes in young Malaysian IDDM patients

The present study was designed to explore the relationship between lipid peroxidation and antioxidant enzymes in young Malaysian insulin dependant diabetes mellitus (IDDM) patients. Indicative parameters of lipid peroxidation, activities of antioxidant enzymes and diabetes parameters were evaluated in single blood samples from 30 young type 1 diabetic patients and 30 healthy control subjects. Antioxidant enzymes namely superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were significantly decreased while plasma malondialdehyde (MDA), an indicator for lipid peroxidation was significantly increased in IDDM patients compared to control subjects. Positive correlations between HbA1c and MDA; fasting blood glucose (FBG) and MDA and negative correlations between HbA1c and SOD; MDA and SOD were observed in these patients. No significant correlation existed between HbA1c and fasting blood glucose, GPx or CAT in the diabetic patients. The strong correlations found between lipid peroxidation, antioxidant enzymes and diabetes parameters confirms the existence of oxidative stress in our IDDM patients.     

Influences of aspartate on circadian patterns of lipid peroxidation products and antioxidants in Wistar rats

INTRODUCTION: The aim of the present study was to demonstrate the effects of aspartate on the circadian patterns of lipid peroxidation products and antioxidants in aspartate-treated rats, in order to investigate the influences of aspartate and whether it could modulate these rhythms differently, since aspartate is an important excitatory neurotransmitter (present in retinohypothalamic tract and suprachiasmatic nuclei [SCN]) involved in the generation and regulation of circadian rhythmicity. METHODS: Aspartate (50 mg/kg body weight) was administered orally for 60 days to Wistar rats, and 24-hour rhythms of thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase were studied under semi-natural (light/dark 12:12 hr) conditions. RESULTS: Exogenous aspartate administration caused acrophase advances in TBARS rhythms, and delays in GSH, SOD and catalase rhythms; altered MESOR and decreased amplitude values were also seen in all of these rhythms. CONCLUSION: Our findings indicate that the orally-treated aspartate could reach the hypothalamus, and various brain centres possibly including SCN, and could modulate the circadian patterns of lipid peroxidation products and antioxidants.     

The effect of arthroscopic surgery and intraarticular drug injection to the antioxidation system and lipid peroxidation at osteoarthritis of knee

OBJECTIVE: To elucidate the effects of arthroscopy and intraarticular hyaluronan therapies on antioxidant enzyme activity and lipid peroxidation. METHODS: This study was conducted between January and May 2005, at the Department of Orthopedics and Traumatology, Suleyman Demirel University, Isparta, Turkey. A total of 60 patients with knee pain who were diagnosed as knee osteoarthritis according to the American College of Rheumatology (ACR) criteria were included in this study and randomly and equally divided into 4 groups. Intraarticular Hylan G-F 20 treatment was given to group 1 for 3 weeks. Intraarticular Hylan G-F 20 treatment plus oral vitamin E were administered to group 2 for 3 weeks. Only arthroscopy treatment was applied to the control group (group 3). Intraarticular Na-hyaluronate treatment was given to group 4 for 5 weeks. Blood and synovial fluid samples were taken from all the patients for biochemical analysis, and the following parameters were measured: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and malondialdehyde (MDA). RESULTS: There was no significant difference in blood SOD, CAT, GPx levels, and synovial SOD and GPx levels in groups treated with hyaluronan, when compared with the controls. However, there was a significant change in MDA levels in synovial fluid samples of group 1, group 2, and group 4, when compared with the controls. CONCLUSION: In knee osteoarthritis, intraarticular hyaluronan therapy following arthroscopy may diminish lipid peroxidation in synovial fluid.     

Lack of changes in the concentration of thiobarbituric acid-reactive substances (TBARS) and in the activities of erythrocyte antioxidant enzymes in alcohol-dependent patients after detoxification.

BACKGROUND: Chronic alcohol consumption induces an increase in oxidative stress. There are studies that indicate changes in the activity of antioxidant enzymes and the concentration of lipid peroxidation products in the blood of organisms as a result of alcohol consumption, but published results are somewhat conflicting. The aim was to study the effect of alcohol withdrawal and detoxification on the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) in erythrocytes and on the concentration of thiobarbituric acid-reactive substances (TBARS) in the erythrocytes and blood plasma of alcohol-dependent patients. MATERIAL/METHODS: The study group consisted of 42 alcohol-dependent men who underwent seven-day detoxification at an addiction treatment department. The control group consisted of 20 healthy male volunteers. Blood for analysis was taken from the antecubital vein before detoxification, after the first detoxification, and after several (> or =3) detoxifications. RESULTS: Before detoxification, the concentration of TBARS in the men's blood plasma was 40% higher (p<0.001) than in the control group. After detoxification, the concentration of TBARS decreased, but remained higher than in the control group. The activities of SOD and GPx in erythrocytes of alcohol-dependent persons were statistically significantly lower than in the control group both before and after detoxification. However, after detoxification, a slight increase in the activity of SOD was observed compared to that before detoxification. CONCLUSIONS: This study confirms that alcohol-dependency leads to oxidative stress in the peripheral blood. Seven-day alcohol withdrawal and detoxification has no effect on TBARS concentration and antioxidant enzyme activity.     

癫痫患者血浆及红细胞中抗氧化剂水平的测定

目的：探讨癫痫疾病状态下血浆及红细胞（RBC）中抗氧化剂水平的变化。方法：测定39例正常对照组、32例癫痫疾病组和26例癫痫治疗组的RBC内谷胱甘肽还原酶(GR)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)、红细胞膜过氧化脂质代谢产物丙二醛（MDA）、红细胞溶血百分率以及血浆中铜蓝蛋白（CER）、维生素A,C,E(VitA、VitC、VitE)等。结果：疾病组的红细胞MDA,溶血百分率,GSH-Px,CAT及血浆CER显著高于对照组（P<0.05），红细胞GR、SOD和血浆VitA，VitC，VitE显著低于对照组（P<0.05,其中红细胞GR，血浆维生素C,P<0.01）；治疗组的红细胞GR,SOD，血浆VitC,VitE均显著高于疾病组(P<0.05,其中GR,P<0.01)。结论：提示采取抗氧化保护措施对患者的治疗有重要意义。     

锰尘对工人血旨质过氧化、抗氧化酶及金属元素的影响

目的：探讨长期暴露于锰尘对工人血脂质过氧化、抗氧化酶及金属元素的影响。方法：测定了60名接触锰尘(接锰)工人和25名对照工人血浆总超氧化物歧化酶(SOD)、CuZn—SOD、谷胱甘肽过氧化物酶((GSH—Px)、过氧化氢酶(CAT)活力、丙二醛(MDA)以及全血Zn、Cu、Fe、Ca、Mn和Mg含量。结果：作业环境空气锰尘中MnO2平均浓度为0．38mg／m^3(0．02～1．51mg／m^3)，样品超标率为50％。接锰工人血浆总SOD活力明显高于对照工人，GSH—Px活力和全血Mg含量明显低于对照工人。结论：过量锰能使机体脂质过氧化增加，并干扰机体其它金属元素的正常代谢。     

谷胱甘肽对KLE与HEC 1 b子宫内膜癌细胞生长的影响

目的比较还原型谷胱甘肽（GSH）对KLE和HEC 1 b子宫内膜癌细胞生长的影响,并探讨其机制。方法①用GSH处理KLE和HEC 1 b子宫内膜癌细胞,噻唑蓝（MTT）法检测两型细胞的增殖水平;②用5 mmol/L GSH处理KLE和HEC 1 b子宫内膜癌细胞,流式细胞仪检测细胞内活性氧（ROS）水平,酶标仪检测细胞内GSH和氧化型谷胱甘肽（GSSG）水平,分光光度计检测细胞内超氧化物歧化酶（SOD）、过氧化氢酶（CAT）及谷胱甘肽过氧化物酶（GSH Px）活性。结果在不进行处理的情况下,KLE子宫内膜癌细胞较HEC 1 b子宫内膜癌细胞的ROS水平及CAT活性较低,GSH水平、GSH/GSSG比值及SOD和GSH Px活性较高（P＜0.01）。用GSH处理后,KLE子宫内膜癌细胞生长加快,细胞内ROS和GSH水平及GSH/GSSG比值下降,SOD、CAT和GSH Px活性降低（P＜0.01）;HEC 1 b子宫内膜癌细胞生长被抑制,细胞内ROS、GSH及GSSG水平下降,GSH/GSSG比值明显升高,CAT和GSH Px活性降低（P＜0.01）。结论GSH对两种子宫内膜癌细胞生长有明显不同的影响,与两种细胞内ROS水平和抗氧化系统活性有密切的关系。     

Cigarette smoke induced oxidative insult in local population of Pokhara.

Objectives: To assess the effect of cigarette smoking on lipid peroxidation induced oxidative stress, antioxidants, uric acid and blood sugar in normal subjects. Methods: The study included 61 normal subjects with regular smoking habit and 57 never-smokers normal subjects matched in respect to socio-economic status, age and BMI. Information regarding smoking habit and other personal details were collected by oral questionnaire. Total antioxidant activity (TAA), reduced glutathione (GSH), alpha-tocopherol (alpha-T), ascorbic acid (AA), uric acid (UA), plasma and urinary thiobarbituric acid reactive substances (TBARS), fasting blood sugar (FBS) and urinary creatinine (Cr) were estimated by standard procedures in both the groups. Ferric Reducing Antioxidant Power (FRAP) procedure is used to estimate TAA which measures total dietary antioxidants. Statistical analysis was done with SPSS version 10. Results: The mean pack years smoked by smokers was 14.4 +/- 15.8. The plasma TBARS level in smokers and never-smokers was 2.6 +/- 0.8 and 2.5 +/- 0.6 micromol/L respectively. The respective figure for urinary TBARS level was 4.6 +/- 2.7 and 3.7 +/- 1.4 micromol/gmCr. Smokers did not show any significant difference from never-smokers with respect to GSH, alpha-T, AA, plasma TBARS and FBS. However, the smokers had significantly lower levels of TAA (p<0.05) and raised level of urinary TBARS (p<0.05) and uric acid (p<0.01) as compared to never-smokers. Conclusion: Our study suggests that smoking induces mild lipid peroxidation but the body is able to compensate for it by removing its adducts. Importantly it also indicates enhanced oxidation of purines which are essential components of both DNA and RNA. Dietary antioxidants are consumed to scavenge free radicals (FR) and other reactive species (RS) in smoke. Female smokers are more prone to oxidative insult than male smokers. In summary RS present in smoke induce mild lipid peroxidation but are not the major contributors of redox imbalance in smoke induced toxicity in the selected subjects. Key words: Tobacco, Smoking, Free radicals, Oxidative stress, Antioxidants.     

Lead Induced Oxidative Stress： Beneficial Effects of Kombucha Tea

Objective To evaluate the effect of oral administration of Kombucha tea (K-tea) on lead induced oxidative stress. Methods Sprague Dawley rats were administered 1mL of 3.8% lead acetate solution daily alone or in combination with K-tea orally for 45d, and the antioxidant status and lipid peroxidation were evaluated. Results Oral administration of lead acetate to rats enhanced lipid peroxidation and release of creatine phosphokinase and decreased levels of reduced glutathione (GSH) and andoxidant enzymes (superoxide dismutase, SOD and glutathione peroxidase, GPx). Lead treatment did not alter humoral immunity, but inhibited DTH response when compared to the control.Lead administration also increased DNA fragmentation in liver. Oral administration of Kombucha tea to rats exposed to lead decreased lipid peroxidation and DNA damage with a concomitant increase in the reduced glutathione level and GPx activity. Kombucha tea supplementation relieved the lead induced immunosuppression to appreciable levels. Conclusion The results suggest that K-tea has potent antioxidant and immunomodulating properties.     

Evaluation of In Vitro Enzymatic and Non-Enzymatic Antioxidant Properites of Leaf Extract from Alpinia Purpurata (Vieill.) K. Schum.

Objective: To evaluate the enzymatic and non-enzymatic antioxidants of leaf extract from Alpinia purpurata. Methods: One gram of fresh leaf of Alpinia purpurata was grinded in 2 mL of 50% ethanol and centrifuged at 10,000×g at 4 ℃ for 10 min. The supernatant obtained was used within 4 h for various enzymatic antioxidants assays like superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx), glutathione S-transferase(GST), ascorbate oxidase, peroxidase, polyphenol oxidase(PPO) and non-enzymatic antioxidants such as vitamin C, total reduced glutathione(TRG) and lipid peroxidation(LPO). Results: The leaf extract of Alpinia purpurata possess antioxidants like vitamin C 472.92±6.80 μg/mg protein, GST 372.11±5.70 μmol of 1-chloro 2,4 dinitrobenzene(CDNB)-reduced glutathione(GSH) conjugate formed/min/mg protein, GPx 281.69±6.43 μg of glutathione oxidized/min/mg protein, peroxidases 173.12±9.40 μmol/g tissue, TRG 75.27±3.55 μg/mg protein, SOD 58.03±2.11 U/mg protein, CAT 46.70±2.35 μmol of H_2O_2 consumed/min/mg protein in high amount whereas ascorbate oxidase 17.41±2.46 U/g tissue, LPO 2.71±0.14 nmol/L of malondialdehyde formed/min/mg protein and PPO 1.14±0.11 μmol/g tissue in moderate amount. Conclusion: Alpinia purpurata has the potential to scavenge the free radicals and protect against oxidative stress causing diseases. In future, Alpinia purpurata may serve as a good pharmacotherapeutic agent.     

铅致幼鼠脑脂质过氧化的实验研究

目的 :探讨铅致发育期中枢神经系统脂质过氧化作用。方法 :通过大鼠孕期及哺乳期不同剂量染铅及用维生素E(VitaminE ,VE)干预所致幼鼠模型 ,测定出生后第 7,2 1d幼鼠脑的脂质过氧化产物 (lipidperoxidativeproduct ,LPO)水平 ,谷胱甘肽 (glutathione ,GSH)含量及超氧化物歧化酶 (superoxidedismutase ,SOD)、谷胱甘肽过氧化物酶 (glutathioneperoxidase ,GSH PX)、过氧化氢酶 (catalase ,CAT)活力。 结果 :无论出生后 7d还是 2 1d ,中、高剂量染铅组幼鼠脑组织LPO水平升高、SOD活力下降 (P <0 .0 5 ,P <0 .0 1) ;出生后 7d幼鼠高剂量染铅组GSH含量下降 (P <0 .0 5 ) ;出生后 2 1d中剂量染铅组CAT活力下降 (P <0 .0 5 ) ;且VE拮抗了这种脂质过氧化作用 (P <0 .0 5 )。结论 :铅可能通过增强脑组织脂质过氧化过程而致发育期神经系统的损伤。     

Redox status in the sentinel lymph node of women with breast cancer

Background: Lymphatic metastasis is regulated in multiple steps including the transit of tumor cells via the lymphatic vessels and the successful seeding in draining lymph nodes. Thus, several molecular signals and cellular changes must be involved in this complex process to facilitate tumor cell entry, colonization, and survival in the lymph node. To our knowledge, the present work explores, for the first time in the literature, the redox status (oxidative stress parameters and enzymatic and non-enzymatic antioxidant defense systems) in the sentinel lymph node (SLN) of women with breast cancer.

Patients and methods: SLNs from 75 women with breast cancer were identified using the one-step nucleic acid amplification (OSNA) method as negative (n?=?43), with micrometastases (n?=?13), or with macrometastases (n?=?19). It will allow us to gain knowledge about the pro-oxidant/antioxidant mechanisms involved in the processes of distant metastases in breast cancer and also to assess whether these parameters may be alternative techniques for staging.

Results: We found different levels of lipid peroxidation in SLNs with micrometastases (increased) and macrometastases (decreased), a decrease in carbonyl group content in SLNs with macrometastases only, and an increase in total antioxidant capacity (TAC) in SNLs with micrometastases and macrometastases. A decrease in the levels of reduced glutathione (GSH) also appears in the SLNs with macrometastases only. Finally, we show increased levels of superoxide dismutase (SOD) and catalase (CAT) activity in SLNs with micrometastases and macrometastases, and decreased levels of glutathione peroxidase (GPx) activity in SNLs with macrometastases but not with micrometastases.

Conclusions: Redox status of lymph node microenvironment participates in the progression of metastatic breast cancer.     

黄连解毒汤有效部位对多发性脑梗塞大鼠脂质过氧化损伤的影响

目的探讨黄连解毒汤有效部位对多发性脑梗塞(MCI)大鼠脂质过氧化损伤的保护作用.方法采用同种大鼠无菌自然干燥血凝块颈总动脉注射建立MCI模型,观察黄连解毒汤有效部位对MCI大鼠脑组织丙二醛(MDA)、超氧化物歧化酶(SOD)、铜锌超氧化物歧化酶(Cu-Zn-SOD)、还原型谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-PX)、谷胱甘肽S转移酶(GSH-ST)、过氧化氢酶(CAT)的影响;结果黄连解毒汤有效部位可提高模型动物脑匀浆SOD、Cu-ZnSOD、CAT、GSH-PX和GSH-ST活性;提高GSH含量,降低MDA、LD含量与LDH活性(P＜0.05,P＜0.01).结论黄连解毒汤有效部位对多发脑梗塞大鼠脑脂质过氧化损伤有显著保护作用.     

Nephroprotective effect of electrolyzed reduced water against cisplatin-induced kidney toxicity and oxidative damage in mice

Background

Cisplatin is a potent chemotherapeutic drug for cancer therapy, but it has serious side effects in clinical treatment, particularly nephrotoxicity. The purpose of this study was to evaluate the protective effect of electrolyzed reduced water (ERW) on renal injury caused by cisplatin.

海带多糖抗脂质过氧化作用的研究

目的 :研究海带多糖 (LJP)对小鼠脑组织脂质过氧化反应的影响。方法 :将小鼠分为灌胃生理盐水的对照组及灌胃 4个不同剂量海带多糖的给药组 ,连续灌胃 10d后 ,以密闭缺氧实验观察在常压缺氧条件下 ,小鼠脑组织中脂质过氧化产物 (LPO)的含量变化情况 ,比较几种主要抗氧化酶的活性。结果 :不同剂量的海带多糖均能有效降低LPO含量 ,提高抗氧化酶的活性 ,以 5 0mg·kg- 1 剂量组效果最好 (P <0 .0 1)。结论 :海带多糖具有抗脂质过氧化作用。     

红外线对大鼠睾丸脂质过氧化作用的影响

目的通过研究红外线对大鼠睾丸脂质过氧化作用,探讨红外线对睾丸生殖功能的影响。方法采用不同波段的红外线对大鼠睾丸局部照射,并与对照组进行比较,研究不同组别睾丸组织超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、谷胱甘肽-S-转移酶（GST）的活性及丙二醛（MDA）、谷胱甘肽（GSH）的含量等指标。结果红外线照射大鼠睾丸组织中MDA含量升高、GSH含量下降,各照射组与对照组比较差异均有显著性（P〈0.05,P〈0.01）;SOD、GSH-Px及GST活性均降低,与对照组比较差异均有统计学差异（P〈0.05,P〈0.01）。结论红外线照射可造成睾丸组织抗氧化能力降低,脂质过氧化作用增强,这可能是红外线对睾丸的生殖功能造成损害的重要原因之一。     

西印度醋栗对四氯化碳引起的大鼠和小鼠急性过氧化肝损伤的保护作用

目的：证实西印度醋栗（Phyllanthus acidus（L.）Skeels）的果实在传统医学中作为保肝药使用的药用用途。方法：连续5d分别给予各组大鼠西印度醋栗果实的70%乙醇提取物（100,200和400mg/kg口服）及对照药水飞蓟素（100mg/kg口服）并于第2天及第3天皮下注射四氯化碳（2mL/kg）。测定血清天冬氨酸氨基转移酶（aspartate transaminase,AST）、丙氨酸氨基转移酶（alanine transaminase,ALT）、碱性磷酸酶（alkaline phosphatase,ALP）、总胆红素以及总蛋白并进行肝脏组织病理学检测。检测大鼠肝组织匀浆中的氧化应激标记物如脂质过氧化反应（lipid peroxidation,LPO）、还原型谷胱甘肽（reduced glutathione,GSH）、超氧化物歧化酶（superoxide dismutase,SOD）、过氧化氢酶（catalase,CAT）以及谷胱甘肽过氧化物酶（glutathione peroxidase,GPx）以衡量西印度醋栗的体外抗氧化作用。此外,本实验还记录了伊维巴诱导的给药后小鼠睡眠时间,使用2,2-二苯基-1-苦基肼（2,2-diphenyl-1-picrylhydrazil,DPPH）法测定小鼠服药后的自由基清除率。结果：与对照组相比,西印度醋栗果实的乙醇提取物及水飞蓟素均显著降低了模型大鼠血清AST、ALT和ALP水平及LPO并升高了TP、GSH、SOD、CAT和GPx的水平（P〈0.01或P〈0.05）。大鼠肝脏组织的组织病理学切片结果也证实了以上实验结果。在小鼠实验中,与对照组相比,西印度醋栗果实的乙醇提取物显著缩短了伊维巴诱导的小鼠睡眠时间（P〈0.01）,并具有较高的DPPH清除率。结论：本研究的实验结果证实了西印度醋栗果实对四氯化碳引起的大鼠急性过氧化肝损伤的保护作用,机制可能与其抗氧化作用及自由基清除功能有关     

Exposure to static magnetic field of pregnant rats induces hepatic GSH elevation but not oxidative DNA damage in liver and kidney

BACKGROUND: The present investigation was designed to evaluate the effects of subacute exposure to static magnetic field (SMF) on some parameters indicative of oxidative stress and on oxidative DNA damage in pregnant rat. METHODS: Females rats (n = 6) were exposed to a SMF (128 mT; 1 h/day) from day 6 to day 19 of pregnancy and were allowed to deliver normally. The control group (n = 6) was not exposed to SMF. Dams were sacrificed 3 days after delivery. The effects of subacute exposure to SMF on oxidative states were assessed on the measurements of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione (GSH) and catalase (CAT). The level of 8-oxo-dG was measured using high-performance liquid chromatography coupled to electrochemical detection. RESULTS: Subacute exposure to SMF failed to alter plasma GPx, MDA, CAT and SOD respectively in liver and kidney. By contrast, SMF increased total GSH (+56%, p <0.05) and reduced GSH (+108%, p <0.05) in liver. Our results showed that the exposure to SMF did not induce oxidative DNA lesions in liver and kidney. CONCLUSIONS: The data do not provide evidence that subacute SMF exposure causes DNA damage in liver and kidney in pregnant rats. The present results suggest that hepatic GSH plays an important role in protection against SMF during pregnancy. These changes in antioxidant status (GSH) lead to some adaptive responses due to activation of systems controlling the body oxidative mechanism balance.     

N-乙酰半胱氨酸预防大鼠模拟空气反复潜水后的免疫抑制

目的 :观察 N-乙酰半胱氨酸 (NAC)对大鼠模拟空气反复潜水后免疫抑制的预防作用。方法 :以添加 NAC的饲料喂养大鼠 6 d,第 4天开始以 70 0 k Pa压缩空气反复暴露 3d,每天 2次每次 6 0 m in(8:0 0～ 9:0 0 ,14 :0 0～ 15 :0 0 )。于暴露后次日晨断头取血及脾脏。用流式细胞术测定外周血及脾淋巴细胞亚型 ,用 3H- Td R掺入法测定脾淋巴细胞增殖反应 ,以 EL ISA法测定循环血浆及脾淋巴细胞经刀豆球蛋白 (Con A)刺激后分泌的 IL - 2水平以及比色法测定血浆中超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、谷胱甘肽过氧化物酶 (GPx)、谷胱甘肽 S转移酶 (GST)的活力和还原型谷胱甘肽 (GSH )、丙二醛 (MDA )的含量。结果 :添加 NAC的食物对外周血及脾淋巴细胞亚型变化无显著影响 ;食物中添加 0 .2 %的 NAC后 ,血浆及脾淋巴细胞经 Con A刺激后分泌的 IL - 2水平及脾淋巴细胞增殖率比正常食物组有明显提高 (P<0 .0 5 ,P<0 .0 1) ,血浆抗氧化酶活力及GSH浓度明显高于对照组 (P<0 .0 5 ) ,而 MDA则显著减少 (P<0 .0 5 )。 结论 :食物中 NAC对高压空气反复暴露引起大鼠的免疫抑制有一定预防作用     

砷致小鼠脂质过氧化及锌、硒对其的干预研究

目的：探讨砷对小鼠血清及肝脏脂质过氧化的影响及锌、硒对其的干预作用。方法：采用亚慢性毒性实验方法,实验结束后分别测定小鼠血清、肝脏组织中脂质过氧化产物丙二醛（MDA）和谷胱甘肽S-转移酶（GST）含量及超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）的活力,同时观察锌、硒干预后对染砷小鼠体内抗氧化水平的影响。结果：随着砷染毒剂量的增加,小鼠血清及肝脏组织中MDA和GST含量显著增加,SOD、GSH-Px活性显著降低,且呈剂量-效应关系。锌、硒能抑制MDA和GST生成量,提高SOD、GSH-Px活性。结论：砷可致机体脂质过氧化,锌、硒可拮抗其脂质过氧化作用。